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| 1 | MicroRNA signature in patients with hepatocellular carcinoma associated with type 2 diabetes显示文摘BACKGROUND Nonalcoholic steatohepatitis-related cirrhosis is one of the liver complications in type 2 diabetes mellitus(T2DM)and reported to be a risk factor for developing hepatocellular carcinoma(HCC).A reliable screening biomarker of liver cirrhosis(LC)and HCC among T2DM patients is important to reduce the morbidity and mortality of this disease.MicroRNA(miRNA)is considered a key player in HCC and T2DM,and it might be a hidden culprit in diabetes-associated HCC,making it a promising reliable prognostic tool.AIM To investigate the signature of serum miRNAs as early biomarkers for the screening of HCC among diabetic patients.METHODS Expression profiles of miRNAs in serum samples of diabetic LC and diabetic HCC patients were assessed using Illumina sequencing;then,RT-qPCR was used to validate significantly altered miRNAs between the two groups.Candidate miRNAs were tested in serum samples of 200 T2DM patients,270 LC patients,200 HCC patients,and 225 healthy control subjects.Additionally,receiver operating characteristic(ROC)analysis,with area under the curve(AUC),was performed to assess the diagnostic performance of the screened miRNAs for discriminating HCC from LC and nonmalignant patients(LC+T2DM).RESULTS Expression of the sequenced miRNAs in serum was different in HCC vs LCpositive T2DM patients.Two miRNAs(miR-34a,miR-221)were significantly upregulated and five miRNAs(miR-16,miR-23-3p,miR-122-5p,miR-198,miR-199a-3p)were significantly down-regulated in HCC compared to LC patients.Analysis of ROC curve demonstrated that the combination of these seven miRNAs can be used as a reliable biomarker for detection of HCC in diabetic patients,as it could identify HCC with high diagnostic accuracy in diabetic LC patients(AUC=0.993)and in diabetic nonmalignant patients(AUC=0.961).CONCLUSION This study validates a panel of serum miRNAs that can be used as a reliable noninvasive screening biomarker of HCC among T2DM cirrhotic and noncirrhotic patients.The study recommends further research to shed light on a possible role of c-Met in T2DM-associated HCC via the miRNA regulatory pathway. | Moustafa Nouh Elemeery Marwa Anwar Mohamed Marwa Ahmed Madkour Mohammed Mohammed Shamseya Noha Mahmoud Issa Ahmed Noah Badr Doaa Ahmed Ghareeb Cheol-Ho Pan | 2019 | World Journal of Gastroenterology2019，25，42： | 3 |
| 2 | Giant mesenteric fibromatosis:Report of a case and review of the literature显示文摘Mesenteric fibromatosis poses a diagnostic and therapeutic challenge.This paper presents a 35-year-old female complaining of vague abdominal pain of 2 mo duration.Her computed tomography scan and magnetic resonance imaging revealed a pelvi-abdominal heterogenous mass with significant displacement of the small bowel and urinary bladder.She underwent surgical excision of the mass with resection and anastomosis of the involved loop of the small intestine.Histological examination confirmed mesenteric fibromatosis without infiltration of the bowel.The patient remained well during the 6 mo follow-up. | Mohammed Khalid Mirza Gari Salman Yousuf Guraya Amir Mounir Hussein Moustafa Mahmoud Nafady Hego | 2012 | World Journal of Gastrointestinal Surgery2012，4，3： | 3 |
| 3 | Ultrafine Fraction and Aerosol Attached Activity Size Distribution of Radon Progeny in Living Room显示文摘 | Amer Mohamed Mohamed Moustafa lotfy Abd EI-Hady Mona Moustafa Mahmoud Mostafa Yuness Abd El Fatah | 2012 | Journal of Physical Science and Application2012，2，7： | 2 |
| 4 | Triazoles and fused triazoles, III: Facile and efficient synthesis of 2,5-disubstituted-s-triazolo[3,4-b]-1,3,4-thiadiazoles显示文摘 | Ali A. El-Emam Mohamed A. Moustafa Hussein I. El-Subbagh Mahmoud B. El-Ashmawy | 1990 | Monatshefte für Chemie Chemical Monthly (-)1990，，2： | 1 |
| 5 | Immunohistochemical investigations of the autonomous nerve distribution in the testis of the camel ( Camelus dromedarius )显示文摘 | Abdel-Mohaimen Moustafa Saleh Mohamed Ahmed Alameldin Mohamed Elmohrezy Abdelmoniem Eman Mahmoud Hassouna Karl-Heinz Wrobel | 2002 | Annals of Anatomy2002，，3： | 1 |
| 6 | Gene expression of 49 kDa apyrase,cytoskeletal proteins,ATPase,ADPase and amino acid contents of Pisum sativum(L.)cells germinated in Euryops arabicus(Steud.ex Jaub.&Spach)water extract显示文摘The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases,cytoskeletal proteins,ATPases,ADPase and amount of amino acid of pea(Pisum sativum L.var.Alaska).Pellets of cytoskeletals proteins(27000 xg)were probed with anti-apyrase antibody,biotinylated anti-rat,actin and alpha and beta-tubulin for Western blotting.ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate.By 72 hours,the abundance of apyrases,cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E.arabicus extracts were sharply increased than those sown in distilled water.All the samples exhibited that the stems had more amount from apyrases,cytoskeletal proteins,amino acids and ATPase and ADPase activities than primary leaves and primary roots that were germinated either on E.arabicus water extract or in distilled water.Based on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins,E.arabicus water extract had an important effect on plant germinations. | MAHMOUD MOUSTAFA SAAD ALAMRI HOIDA ZAKI NAGLAA LOUTFY TAREK TAHA ALI SHATI MOHAMED ALKAHTANI SAJDA SIDDIQI | 2019 | BIOCELL2019，43，2： | 1 |
| 7 | Using OPC technology to support the study of advanced process control显示文摘 | Magdi S. Mahmoud Muhammad Sabih Moustafa Elshafei | 2014 | ISA Transactions2014，，： | 1 |
| 8 | Analysis of semiconductor laser dynamics under gigabit rate modulation显示文摘 | Moustafa Ahmed Minoru Yamada Safwat W Z Mahmoud | 2007 | J Appl Phys2007，101，03： | 1 |
| 9 | Immunohistochemical investigations of the autonomous nerve distribution in the testis of the camel ( Camelus dromedarius )显示文摘 | Abdel-Mohaimen Moustafa Saleh Mohamed Ahmed Alameldin Mohamed Elmohrezy Abdelmoniem Eman Mahmoud Hassouna Karl-Heinz Wrobel | 2002 | Annals of Anatomy2002，，3： | 1 |
| 10 | Prevalence of Fasciola infection among school children in Sharkia Govemorate, Egypt 显示文摘 | Hassan MM Moustafa NE Mahmoud LA | 1995 | J Egypt Soc Parasilol1995，25，2： | 1 |
| 11 | Use of a covariance variogram to investigate influence of subsurface drainage on spatial variability of soil-water properties显示文摘 | Mahmoud M Moustafa Atsushi Yomota | 1998 | Agricultural water management1998，37，： | 1 |
| 12 | Plant Nitrogen Metabolism: Balancing Resilience to Nutritional Stress andAbiotic Challenges显示文摘Plant growth and resilience to abiotic stresses,such as soil salinity and drought,depend intricately on nitrogen metabolism.This review explores nitrogen’s regulatory role in plant responses to these challenges,unveiling a dynamic interplay between nitrogen availability and abiotic stress.In the context of soil salinity,a nuanced rela-tionship emerges,featuring both antagonistic and synergistic interactions between salinity and nitrogen levels.Salinity-induced chlorophyll depletion in plants can be alleviated by optimal nitrogen supplementation;however,excessive nitrogen can exacerbate salinity stress.We delve into the complexities of this interaction and its agri-cultural implications.Nitrogen,a vital element within essential plant structures like chloroplasts,elicits diverse responses based on its availability.This review comprehensively examines manifestations of nitrogen deficiency and toxicity across various crop types,including cereals,vegetables,legumes,and fruits.Furthermore,we explore the broader consequences of nitrogen products,such as N_(2)O,NO_(2),and ammonia,on human health.Understand-ing the intricate relationship between nitrogen and salinity,especially chloride accumulation in nitrate-fed plants and sodium buildup in ammonium-fed plants,is pivotal for optimizing crop nitrogen management.However,prudent nitrogen use is essential,as overapplication can exacerbate nitrogen-related issues.Nitrogen Use Effi-ciency(NUE)is of paramount importance in addressing salinity challenges and enhancing sustainable crop productivity.Achieving this goal requires advancements in crop varieties with efficient nitrogen utilization,pre-cise timing and placement of nitrogen fertilizer application,and thoughtful nitrogen source selection to mitigate losses,particularly urea-based fertilizer volatilization.This review article delves into the multifaceted world of plant nitrogen metabolism and its pivotal role in enabling plant resilience to nutritional stress and abiotic challenges.It offers insights into future directions for sustainable agriculture. | Muhammad Farhan Manda Sathish Rafia Kiran Aroosa Mushtaq Alaa Baazeem Ammarah Hasnain Fahad Hakim Syed Atif Hasan Naqvi Mustansar Mubeen Yasir Iftikhar Aqleem Abbas Muhammad Zeeshan Hassan Mahmoud Moustafa | 2024 | Phyton-International Journal of Experimental Botany2024，93，3： | 0 |
| 13 | Cloning and characterization of 66 kDa streptavidin-binding peptides(SBP)of Pisum sativum L.embryo specific to var.Alaska显示文摘The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide(SBP)found in the germinated embryos of Pisum sativum L.var.Alaska.The pea(P.sativum var.Alaska)embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos,but not in the cotyledons.The total RNA was isolated from embryos of P.sativum but could not be isolated from the cotyledons.The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk(P.sativum var.Alaska)were cloned and identified using pMOSBlue vector.66 kDa(SBP)gene from the embryos of P.sativum var.Alaska possesses 327 bp having an open reading frame(ORF)region in a part of the gene that encoded for 108 amino acids.Alignment showed similarity among 66 kDa SBPs P.sativum var.Alaska,with P.sativum seed biotinylated protein(SBP65)and P.sativum sbp65a mRNA with DNA distance matrix between 0.0094 to 1.2676.MALDI-TOF mass spectrometry analysis of 66 kDa(SBP)proteins showed it had similar short peptides to 19 proteins found in different organisms,especially Convicilin precursor,and the seed biotinylated protein in P.sativum.The alignment results of both nucleotide sequences and amino acid residues either from cloning or MALDI-TOF-MS showed differences with related species,especially P.sativum.No mRNA was found in the cotyledons during seeds germination,which means no metabolic activities and this part may act only as food reservoirs for growing newly embryos. | MAHMOUD MOUSTAFA SAAD ALAMRI TAREK TAHA ALI SHATI SULAIMAN ALRUMMAN MOHAMED ALKAHTANI | 2019 | BIOCELL2019，43，3： | 0 |
| 14 | Biosynthesis of raw starch degradingβ-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater显示文摘The study was sought to enhance the synthesis of thermal stableβ-cyclodextrin glycosyltransferase(β-CGTase)using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production ofβ-cyclodextrin(β-CD)from raw potato starch.Thermophilic bacteria producingβ-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA gene.Alginate-encapsulated cultures exhibited twice-fold ofβ-CGTase production more than free cells.Scanning electron microscopy(SEM)of polymeric capsules indicated the potential for a longer shelf-life,which promotes the restoration of activity in bacterial cells across semi-continuous fermentation ofβ-CGTase production for 252 h.The optimal conditions forβ-CGTase synthesis using potato wastewater medium were at 36 h,pH of 8.0,and 50°C with 0.4%potato starch and 0.6%yeast extract as carbon and nitrogen sources,respectively.The purified enzyme showed a specific activity of 63.90 U/mg with a molecular weight of∼84.6 kDa as determined by SDS-PAGE analysis.The high enzyme activity was observed up to 60°C,and complete stability was achieved at 75°C.High levels of activity and stability were shown at pH 8.0,and the pH range from 7.0–10.0,respectively.The enzyme has an appreciable affinity for raw potato starch with a Km of 5.7×10−6 M and a Vmax of 87.71μmoL/mL/min.β-CD production was effective against 25 U/g of raw potato starch.The outcomes demonstrated its feasibility to develop a fermentation process by integrating the cost-effective production ofβ-CGTase having distinctive properties forβ-CD production with ecofriendly utilization of potato wastewater. | YASSER S.MOSTAFA SAAD A.ALAMRI SULIMAN A.ALRUMMAN TAREK H.TAHA MOHAMED HASHEM MAHMOUD MOUSTAFA LAMIAA I.FAHMY | 2021 | BIOCELL2021，45，6： | 0 |
| 15 | Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens显示文摘Microbial infections cause complicated health influences along with bad economic impacts.In the present investigation,three dominant seaweeds namely,Amphiroa anceps,Corallina officinalis and Sargassum filipendula were collected from different Egyptian sites at the Red Sea and Mediterranean Sea during autumn 2019.Organic extracts of the three algae were screened for their antibacterial activity against three pathogenic bacteria Salmonella typhiimurium,Staphylococcus aureus and Escherichia coli,in addition to in vitro antiviral activity against Rotavirus(RV),and Coxsackie virus B3(CVB3)that cause severe diseases in human.Organic extract of A.anceps,C.officinalis and S.filipendula inhibit E.coli cells by 57.1%,85.7%,and 91.4%,respectively.The highest level of concentration of the C.officinalis extract(100μg/mL)inhibits 100%of Staphylococcus aureus cells followed by S.filipendula and A.anceps extract which inhibit 82.5%and 75%of S.aureus.Similarly,the highest concentration of C.officinalis extract inhibits S.typhiimurium by 80%.The extract of A.anceps exhibited a high antiviral effect against RV infection with TI=22 and virus titers lessened by 2.75 log TCID_(50) followed by extractions of C.officinalis with TI=18.3 and virus titers reduced by 2.5 log TCID_(50).Against CVB3 infection,the extract of A.anceps causes the highest antiviral activity with TI=15 and reduce the viral titers by 2.5 log TCID_(50),followed by extractions of C.officinalis with TI=8.8 and inhibition of virus titers by 1.75 log TCID_(50).Extract of S.filipendula displayed the lowest antiviral effects against RV and CVB3 infection with TI=2.4 and 1.4,respectively.The obtained results clarified that the extract of three marine seaweeds maintains a potent antimicrobial activity,making them a future promising source of new antimicrobial drugs. | JELAN MOFEED MOHAMED DEYAB ABDELNASER MOHAMED MAHMOUD MOUSTAFA SALLY NEGM EMAD EL-BILAWY | 2022 | BIOCELL2022，46，1： | 0 |
| 16 | Secondary antiviral metabolites from fungi with special reference to coronaviruses显示文摘Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various systems.Because of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals,viral contagions are amongst the most causative diseases affecting people worldwide.Fungi exemplify a massive source of bioactive molecules as,many fungal secondary metabolities like Oxoglyantrypine,Carneic acid F,Scedapin C,Asteltoxin E,Phomanolide,Norquinadoline A and Quinadoline B have antiviral activity.This review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially Coronaviruses moreover several pieces of literature pointed out that many clusters of fungi in different biotopes are waiting to be exploited. | MOHAMED SALEM MOHAMMAD EL-METWALLY WESAMELDIN SABER SALLY NEGM ATTALLA EL-KOTT YASSER MAZROUA ABEER MAKHLOUF MAHMOUD MOUSTAFA | 2022 | BIOCELL2022，46，8： | 0 |
| 17 | Geotectonic significance of the Neoproterozoic ophiolitic metagabbros of Muiswirab area,South Eastern Desert,Egypt:constraints from their mineralogical and geochemical characteristics显示文摘Petrological and geochemical studies of Neo-proterozoic metagabbros were carried out in the Muiswirab area,South Eastern Desert of Egypt.The Muiswirab area comprises of ophiolitic metagabbroic rocks(MOM),which are tectonically thrusted over a thick pile of metavolcanic rocks and intruded by syn-and post-tectonic granitoid rocks.The whole-rock geochemical variations coupled with chemical compositions of mineral constituents are used to attain the genesis and tectonic evolution of the studied metagabbros.The geothermobarometric investiga-tion of the analyzed amphiboles from(MOM)revealed that these metagabbros underwent regional metamorphism under lower to upper greenschist facies(biotite zone)conditions(at a temperature of 450 to 500℃and pressure of 1-3 kbar).Geochemically,the metagabbros(MOM)show tholeiitic affinity and cxhibiting both arc-and MORB-like characters as evidenced by their clinopyrox-ene compositions and the Ti/V ratios(11.84-31.65),which considered as prominent features of forearc tectonic regime.The geochemical features suggest a probable fractionation of olivine±clinopyroxene±plagioclase as well as insignificant crustal contamination.The parental magma of the investigated MOM rocks seems to be developed in a sub-arc mantle wedge setting due to the enrichments of LILE(e.g..Rb,Ba,Sr,Pb)over HFSE(e.g,Ti,Nb,Y,Zr,Hf,Ta).The studied MOM rocks have lower values of Nb/U relative to MORB and OIB indicating that their geochemical variation produced due to the enrichment of a lithosphere mantle by OIB like components.The ratios of Zn/Fet,La/Sm,Sm/Yb,Th/Yb and Nb/Yb indicate that the MOM rocks represent a fragment of oceanic crust originated at a supra-subduction zone environment and their parental magma developed by 5-30%partial melting of a spinel lherzolite mantle rather than pyroxenite in an island arc setting and conformable with most of the Egyptian ophiolitic metagabbros. | Moustafa M.Mogahed Wlaa Mahmoud Saad | 2020 | Acta Geochimica2020，39，6： | 0 |
| 18 | Potential detoxification of aflatoxin B2 using Kluyveromyces lactis and Saccharomyces cerevisiae integrated nanofibers显示文摘Current investigation has shown that human exposure to aflatoxins is not limited to the administration of contaminated cereals,but water is another possible source.This study was aimed to design easily applicable method to eliminate aflatoxin B2(AFB2)from contaminated drinking water.Electrospinning has been used for preparation of probiotic-coated polyvinyl alcohol(PVA)and cellulose acetate(CA)nanofibers.Both of these hybrid nanofibers were studied by scanning electron microscopy(SEM)and Fourier-transformed infrared spectroscopy(FT-IR).SEM showed the proper coating of probiotic strains(Kluyveromyces lactis CBS 2359 and Saccharomyces cerevisiae ATCC 9763)on both nanofiber types.Different areas(1-5 cm^(2))of the probiotic-nanofiber hybrid were used to enhance the removal of 20 ng/ml of aflatoxin B2(AFB2)from prepared AFB2-contaminated water over time.Results revealed that a 5 cm^(2) area of probiotic-coated PVA nanofibers can eliminate 97.5% of AFB2 as compared to 87.5%,90.5%,93.5%,and 95.5%,for 1 cm2,2 cm^(2),3 cm^(2),and 4 cm^(2),respectively,while probiotic-coated CA nanofibers were slightly less effective.Nevertheless,the cytotoxicity of probiotics-CA treated water on cultured human fibroblasts was almost 10 times lower than the cytotoxicity recorded in probiotics-PVA treated water.Therefore,results of the current research suggest that probiotics-polymer nanofiber membranes can be used as an extra stage in the water purification system for the treatment of AFB2-contaminated water. | MAHMOUD MOUSTAFA TAREK TAHA MOHAMED ELNOUBY NEHAL EL-DEEB GAMAL HAMAD M.A.ABUSAIED SULAIMAN ALRUMMAN | 2017 | BIOCELL2017，41，2： | 0 |
| 19 | Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source,Talaromyces purpureogenus KJ584844显示文摘The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this respect.The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process.The interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state fermentation.The optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g tubers.Using Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase stimulants.Co nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the nanoparticles.The findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase catalyst.This approach could be extended to modulate the productivity and activity of similar biomolecules. | SALLY NEGM MOHAMMAD EL-METWALLY WESAM ELDIN SABER SAHAR ABO-NEIMA MAHMOUD MOUSTAFA ATTALLA EL-KOTT | 2021 | BIOCELL2021，45，1： | 0 |
| 20 | Hybrid Single Image Super-Resolution Algorithm for Medical Images显示文摘High-quality medical microscopic images used for diseases detection are expensive and difficult to store.Therefore,low-resolution images are favorable due to their low storage space and ease of sharing,where the images can be enlarged when needed using Super-Resolution(SR)techniques.However,it is important to maintain the shape and size of the medical images while enlarging them.One of the problems facing SR is that the performance of medical image diagnosis is very poor due to the deterioration of the reconstructed image resolution.Consequently,this paper suggests a multi-SR and classification framework based on Generative Adversarial Network(GAN)to generate high-resolution images with higher quality and finer details to reduce blurring.The proposed framework comprises five GAN models:Enhanced SR Generative Adversarial Networks(ESRGAN),Enhanced deep SR GAN(EDSRGAN),Sub-Pixel-GAN,SRGAN,and Efficient Wider Activation-B GAN(WDSR-b-GAN).To train the proposed models,we have employed images from the famous BreakHis dataset and enlarged them by 4×and 16×upscale factors with the ground truth of the size of 256×256×3.Moreover,several evaluation metrics like Peak Signal-to-Noise Ratio(PSNR),Mean Squared Error(MSE),Structural Similarity Index(SSIM),Multiscale Structural Similarity Index(MS-SSIM),and histogram are applied to make comprehensive and objective comparisons to determine the best methods in terms of efficiency,training time,and storage space.The obtained results reveal the superiority of the proposed models over traditional and benchmark models in terms of color and texture restoration and detection by achieving an accuracy of 99.7433%. | Walid El-Shafai Ehab Mahmoud Mohamed Medien Zeghid Anas MAli Moustafa H.Aly | 2022 | Computers, Materials & Continua2022，，9： | 0 |
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