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	题名	作者	年代	出处	被引量
1	Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC.	Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard	2012	World Journal of Gastroenterology2012，18，26：	12
2	A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzopyrene and (+/-)-anti-benzopyrene-7,8-diol 9,10-oxide in cultured human cells显示文摘	Hanelt S Helbig R Hartmann A	1997	Mutat Res1997，390，12：	2
3	Laboratory methods for the diagnosis of Legionella infections显示文摘	Maiwald M Helbig JH Luck PC	1998	J Mierobiol Methods1998，33，1：	1
4	NF- kappaB promotes breast cancer cell migration and metastasis by inducing the expression of the chemokine receptor CXCR4 显示文摘	Helbig G Christopherson KW 2nd Bhat-Nakshatri P	2003	J Biol Chem2003，278，21：	1
5	Occurrence and distribution of sequence types among Legiortella pneumophila strains isolated from patiens in Germany: common features and differences to other regions of the world显示文摘	BORCHARDT J HELBIG J H LUCK P C	2008	Eur J Clin microbial Infect Dis2008，27，1：	1
6	NF-kappaB promotes breast cancer cell migration and metastasis by inducing the expression of the chemokine receptor CXCR4 显示文摘	Helbig G Christopherson K N Bhat-Nakshatri P	2003	J Biol Chem2003，278，21：	1
7	The role of action representation in visual object显示文摘	Helbig H B Graf M Kiefer M		0，，：	1
8	Induction of cell proliferation by fibroblast and insulin-like growth factors in pure rat inner ear epithelial cell cultures显示文摘	Zheng JL Helbig C Gao WQ	1997	J Neurosci1997，17，：	1
9	Rubeosis iridis after vitrectomy for diabetic retinopathy 显示文摘	Helbig H Kellner U Bornfeld N	1998	Graefe''s Arch Clin Exp Ophthalmol1998，236，10：	1
10	NF- kappaB promotes breast cancer cell migration and metastasis by inducing the expression of the chemokine receptor CXCR4显示文摘	Helbig G Christopherson KW 2nd Bhat-Nakshatri P	2003	J Biol Chem2003，278，21：	1
11	Surgery for diabetic retinopathy显示文摘	Helbig H	2007	Ophthalmologica2007，221，2：	1
12	75-plus years of anisotropy in exploration and reservoir seismic : A historical review of concepts and methods 显示文摘	Helbig K Thomsen L	2005	Geophysics2005，70，6：	1
13	Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens显示文摘	Helbig M Frosch P Kern P Frosch M 1993	1993	J Clin Microbiol1993，31，12：	1
14	Bone tissue and plasma con- centrations of linezolid and vancomycin in rabbits with prosthesis-related infection due to MRSA显示文摘	Swoboda S Helbig L Kommerell M	2009	Pharmazie2009，64，6：	1
15	Characterization of Legionella pneumophila isolates from patients in Japanaccording to serogroups, monoclonaiantibody subgroups and sequencetypes显示文摘	Amemura-Maekawa J Kura F Helbig JH	2010	J MedMicrobiol2010，59，：	1
16	Core de- compression combined with impl-antation of a deminer- alised bone matrix for non-traumatic osteonecrosis of the fern-oral head显示文摘	HELBIG L SIMANK H KROEBER M	2012	Arch Orthop Trauma Surg2012，132，8：	1
17	Choroidal thick- ness measurements during central serous chorioretinopathy treatment显示文摘	BRANDL C HELBIG H GAMULESCU MA	2014	Int Ophthalmol2014，34，1：	1
18	Differences in glycemic variability between normoglycemic and prediabetic sub- jects显示文摘	HA NEFELD M SULK S HELBIG M	2014	J Diabetes Sci Technol2014，8，2：	1
19	Debittering of skim milk hydrolysates by absorption for incorporation into acidic beverage显示文摘	HELBIG N B HO L CHRISTY G E	1980	Journal of Food Science1980，45，：	1
20	Sonography of nasal tip anatomy and surgical tip refinement显示文摘	TASMAN A J HELBIG M	2000	Plast Reconstr Surg2000，105，7：	1

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