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| 1 | Desulfurization metabolite of Rhodococcus erythropolis LSSE8-1 and its related desulfurizational gene fragments显示文摘Rhodococcus erythropolis LSSE8-1 is a newly isolated biodesulfurizaion strain from the soil of Chishui gas field, Guizhou Province, China. The analysis of its metabo-lism product shows that the strain is a kind of biocatalyst able to oxidize dibenzothiophene (DBT) to 2-hydroxydi- phenyl (HBP), and therefore the sulfur in DBT is selectively removed. By using DBTO2 (dibenzothiophene 5,5-dioxide) as substrate, both DBT and HBP are found in the culture, which shows that the reaction from DBT to DBTO2 is re-versible in the cell. While using 0.5 mmol/L DBT as control, 0.01—0.4 mmol/L DBTO2 shows poisonous effect to the cell, which will explain why there is no DBTO2 accumulation in the process of biodesulfurization. After treatment by lysozme, the plasmid DNA of the strain is isolated by alkaline method to be used as the template of PCR reaction. Three dsz gene fragments of 1.3, 1.0 and 1.2 kb respectively were amplified. Each fragment is ligate with PGEM-T vector, and cloned into E. coli. DH5. The clone DNA is sequenced and the result shows that dsz related genes are highly conservative. The identities of dszA and dszB with respect to IGTS8 are 100%, and the identity of dszC with that of IGTS8 is 99%. | GOU Zhongxuan, LUO Mingfang, LI Xin, XING Jianmin & LIU Huizhou Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Liu Huizhou (e-mail: hzliu@ home.ipe.ac.cn) | 2003 | Chinese Science Bulletin2003，48，24： | 5 |
| 2 | Performance of microbiological control by a point-of-use filter system for drinking water purification显示文摘Purification capacity of a faucet mounted type water filter for home use was evaluated,particularly with regard to microbiological performance under different running conditions.Biofilms were formed inside the filter,affecting the bacterial quality of the effuent water.Low flow rate,long stagnation period and high filter temperature were found favorable for bacterial growth inside.By commercial analytical profile index(API) kits,ten different bacterial species were identified in drinking water,four of which were probably contributed to the biofilm formation since they were also present in the biofilm.Fluorescence in situ hybridization(FISH) was used to confirm the API identification results,and direct viable count(DVC) method was employed to improve the sensitivity of FISH for the isolated Acinetobacter spp.and Pseudomonas putida as models.Relationship between the filter operating condition and the bacterial community alteration was partly revealed,which could provide the basic knowledge for the filter design and its practical use. | SU Fengyi LUO Mingfang ZHANG Fei LI Peng LOU Kai XING Xinhui | 2009 | Journal of Environmental Sciences2009，21，9： | 4 |
| 3 | Strain screening, fermentation, separation, and encapsulation for production of nattokinase functional food显示文摘 | WEI Xuetuan LUO Mingfang XIE Yuchun | 2012 | Applied Biochemistry Biotechnology2012，168，： | 1 |
| 4 | Microbial desulfurization of dibenzothiophene and 4,6-dimethyldibenzothiophene in dodecane and straight-run diesel oil显示文摘 | Luo Mingfang Xing Jianmin Gou Zhongxuan Liu Huizhou Chen Jiayong | 2003 | Korean Journal of Chemical Engineering2003，，4： | 1 |
| 5 | Desulfurization of Dibenzothiophene by Lyophilized Cells of Pseudomonas DelafieldiiR - 8 in the Presence of Dodecane显示文摘 | Luo Mingfang Xing Jianmin Gou Zhongxuan | 2003 | Biochem Eng J2003，13，1： | 1 |
| 6 | Optimal ordering policy for platelets:Data-driven method vs model-driven method显示文摘Platelets,one of the most significant materials in treating leukemia,have a limited shelf life of approximately five days.Because platelets cannot be manufactured and can only be centrifuged from whole or donated blood directly,an accurate ordering policy is necessary for the efficient use of this limited blood resource.Given this motivation,the present study examines an ordering policy for platelets to minimize the expected shortage and overage.Rather than using the two-step model-driven method that first fits a demand distribution and then optimizes the order quantity,we solve the issue using an integrated datadriven method.Specifically,the data-driven method works directly with demand data and does not rely on the assumption of demand distribution.Consequently,we derive theoretical insights into the optimal solutions.Through a comparative analysis,we find that the data-driven method has a mean anchoring effect,and the amounts of shortage and overage reduced by this method are greater than those reduced by the model-driven method.Finally,we present an extended model with the service level requirement and conclude that the order decided by the data-driven method can precisely satisfy the service level requirement;however,the order decided by the model-driven method may be either higher or lower than the service level requirement and can lead to a higher cost. | Mingfang Yang Xu Chen Zheng Luo | 2021 | Fundamental Research2021，1，5： | 0 |
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