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| 1 | DNA methylation,microRNAs,and their crosstalk as potential biomarkers in hepatocellular carcinoma显示文摘Epigenetic alterations have been identified as a major characteristic in human cancers.Advances in the field of epigenetics have contributed significantly in refining our knowledge of molecular mechanisms underlying malignant transformation.DNA methylation and microRNA expression are epigenetic mechanisms that are widely altered in human cancers including hepatocellular carcinoma(HCC),the third leading cause of cancer related mortality worldwide.Both DNA methylation and microRNA expression patterns are regulated in developmental stage specific-,cell type specific-and tissue-specific manner.The aberrations are inferred in the maintenance of cancer stem cells and in clonal cell evolution during carcinogenesis.The availability of genome-wide technologies for DNA methylation and microRNA profiling has revolutionized the field of epigenetics and led to the discovery of a number of epigenetically silenced microRNAs in cancerous cells and primary tissues.Dysregulation of these microRNAs affects several key signalling pathways in hepatocarcinogenesis suggesting that modulation of DNA methylation and/or microRNA expression can serve as new therapeutic targets for HCC.Accumulative evidence shows that aberrant DNA methylation of certain microRNA genes is an event specifically found in HCC which correlates with unfavorable outcomes.Therefore,it can potentially serve as a biomarker for detection as well as for prognosis,monitoring and predicting therapeutic responses in HCC. | Sumadi Lukman Anwar Ulrich Lehmann | 2014 | World Journal of Gastroenterology2014，20，24： | 11 |
| 2 | Profile of the breast cancer susceptibility marker rs4245739 identifies a role for miRNAs显示文摘Objective:To determine the influence of the single nucleotide polymorphism(SNP)rs4245739 on the binding and expression of micro RNAs and subsequent MDM4 expression and the correlation of these factors with clinical determinants of ER-negative breast cancers.Methods:Find Tar and miRanda were used to detect the manner in which potential micro RNAs are affected by the SNP rs4245739-flanking sequence.RNA sequencing data for ER-negative breast cancer from The Cancer Genome Atlas(TCGA)were used to compare the expression of miR-184,miR-191,miR-193a,miR-378,and MDM4 in different rs4245739 genotypes.Results:Comparison of ER-negative cancer patients with and without the expression of miR-191 as well as profile micro RNAs(miR-184,miR-191,miR-193a and miR-378 altogether)can differentiate the expression of MDM4 among different rs4245739 genotypes.Although simple genotyping alone did not reveal significant clinical relationships,the combination of genotyping and micro RNA profiles was able to significantly differentiate individuals with larger tumor size and lower number of involved lymph nodes(P<0.05)in the risk group(A allele).Conclusions:We present two novel methods to analyze SNPs within 3′UTRs that use:(i)a single miRNA marker expression and(ii)an expression profile of miRNAs predicted to bind to the SNP region.We demonstrate that the application of these two methods,in particular the miRNA profile approach,permits detection of new molecular and clinical features related to the rs4245739 variant in ER-negative breast cancer. | Sumadi Lukman Anwar Wahyu Wulaningsih Johnathan Watkins | 2017 | Cancer Biology & Medicine2017，14，4： | 3 |
| 3 | hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma显示文摘AIM To screen clinically relevant micro RNAs (mi RNAs) silenced by DNA methylation in human hepatocellular carcinoma(HCC).METHODS Knockdown of DNA methyltransferases (DNMTs) using si RNAs and mi RNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated mi RNA downregulation. Confirmation using individual quantitative real-time PCR (qR T-PCR) assays was thenperformed followed by DNA methylation quantification at the promoter of the mi RNA genes. Quantification of DNA methylation and mi RNA expression was then performed in primary HCC tumor samples and related with clinicopathological variables.RESULTS mi RNA profiling after DNMT knockdown in HCC cell lines revealed upregulation of mi R-23, mi R-25 and mi R-183. After q RT-PCR confirmation and Cp G island methylation quantification of these miR NAs in cell lines, further analysis in primary HCC specimens showed that hsa-mi R-183 is hypermethylated in 30% of HCC (n = 40). Expression of mature miR-183 showed an inverse correlation with DNA methylation levels. In HCC cells, DNMT knockdown and 5-aza-2'-deoxycytidine treatment reduced methylation and stimulated expression of mi R-183. In HCC patients, hypermethylation at hsami R-183 promoter significantly correlates with poor survival (log-rank test P = 0.03). DNA methylation analysis in healthy liver, benign liver tumors (hepatocellular adenoma and focal nodular hyperplasia) and their corresponding adjacent tissues showed absence of hypermethylation supporting the notion that aberrant methylation at hsa-miR-183 is specific for the malignant transformation of hepatocytes.CONCLUSION Our data indicate that hypermethylation of hsa-miR-183 is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker. | Sumadi Lukman Anwar Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Reena Buurman Britta Skawran Ulrich Lehmann | 2017 | World Journal of Gastroenterology2017，23，9： | 3 |
| 4 | What are the key elements of a sustainable university显示文摘 | Rebeka Lukman Peter Glavic | | 0，，09： | 1 |
| 5 | Do we discriminate? 显示文摘 | Crigger NJ Lukman R Galusha C | 2007 | Men Nurs2007，2，5： | 1 |
| 6 | Interregional Inequalities in Indonesia:A Seetoral Decomposition Analysis for 1975-1992 显示文摘 | A R Lukman | 1999 | Bulletin of Indonesian Economic Studies1999，31，2： | 1 |
| 7 | Facile synthesis,stabilization,and anti-bacterial perfor-mance of discrete Ag nanoparticles using Medicago sativa seed exudates显示文摘 | Lukman A I Gong B Marjo C E | 2011 | Colloid Interface Sci2011，353，2： | 1 |
| 8 | Cou- pled eleetrokinetics-adsorption technique for simultane- ous removal of heavy metals and organics from saline- sodic soil 显示文摘 | Lukman S Essa M H Mu'Azu N D Bukhari A | 2013 | The Scientific World Journal2013，2013，34： | 1 |
| 9 | Recurrent laryngeal nerve in thyroid surgery:a critical appraisal 显示文摘 | Hisham AN Lukman MR | 2002 | ANZJ Surg2002，72，12： | 1 |
| 10 | Recurrent laryngeal nerve in thyroid surgery: a critical appraisal 显示文摘 | Hisham AN Lukman MR | 2002 | ANZ J Surg2002，72，12： | 1 |
| 11 | Recurrent laryngeal nerve in thy-roid surgery:acritical appraisal显示文摘 | Hisham AN Lukman MR | | 0，，12： | 1 |
| 12 | Corporate Social Responsibility and En- trepreneurship (CSRE) : Antidotes to Poverty, Insecurity and Underdevelopment in Nigeria 显示文摘 | Lukman Raimi Innocent Akhuemonkhan Olakunle Dare Ogunjirin | 2015 | Social Responsibility Journal2015，，11： | 1 |
| 13 | Freestanding single-walled carbon nanotube/SnO2 anode paper for flexible lithium-ion batteries显示文摘 | Lukman Noerochim Wang Jiazhao Chou Shulei | | 0，，03： | 1 |
| 14 | Utero-vaginal prolapse:a Vural disability of the young显示文摘 | Lukman Y | 1995 | East Afr Med J1995，72，1： | 1 |
| 15 | Early testing of graft incompatibilities in apricot and lemon trees using in vitro techniques显示文摘 | Jonard R Lukman D Schall F | | 0，，1： | 1 |
| 16 | Childhood amblyopia treatment:psychosocial implications for patients and primary cares显示文摘 | Choong YF Lukman H Martin S | 2004 | Eye2004，18，： | 1 |
| 17 | CTX-M producing Escherichia coli isolated from cattle feces in Bogor slaughterhouse,Indonesia显示文摘Objective: To determine the occurrence of CTX-M producing Escherichia coli(E. coli)from cattle feces in Bogor slaughterhouse, Indonesia.Methods: A total of 220 cattle feces samples were collected from Bogor slaughterhouse from March to April 2015. Presence of extended-spectrum beta-lactamase(ESBL) producing E. coli was detected by disc diffusion test based on the recommendation from Clinical and Laboratory Standards Institute(2014). Bacterial strains which were confirmed as producing ESBLs were further analyzed for the presence of bla genes of the ESBL by PCR.Results: The results showed that CTX-M producing E. coli isolates were detected in 19 samples from 220 samples(8.6%). The b-lactamase genes detected were CTX-M-1(n = 10) and CTX-M-9(n = 9). All of the CTX-M producing E. coli isolates showed multidrug resistance phenotypes to at least four antibiotics. The highest incidence of antibiotics resistance was showed to ampicillin(100.0%), cefotaxime(100.0%), and cefpodoxime(100.0%), followed by streptomycin(84.3%), trimethoprim-sulfamethoxazole(73.7%), erythromycin(52.6%), kanamycin(26.3%), doxycycline(10.5%), and ceftazidime(0.0%).Conclusions: Detection of CTX-M-producing E. coli in cattle feces raises important questions as they can represent a potential risk factor to public health. | Mirnawati Bachrum Sudarwanto Denny Widaya Lukman Hadri Latif Herwin Pisestyani Eddy Sukmawinata mer Akineden Ewald Usleber | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，7： | 1 |
| 18 | Recurrent laryngeal nerve in thyroid surgery:a critical appraisal显示文摘 | Hisham AN Lukman MR | 2002 | ANZ J Surg2002，72，12： | 1 |
| 19 | Bone-Targeted Therapies for Elderly Patients with Renal Cell Carcinoma: Current and Future Directions显示文摘 | Thomas Roza Lukman Hakim Hendrik Poppel Steven Joniau | 2013 | Drugs & Aging2013，，11： | 1 |
| 20 | Recurrent laryngeal nerve in thyroid surgery:a critical appraisal显示文摘 | Hisham AN Lukman MR | 2002 | ANZ J Surg2002，72，12： | 1 |
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