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| 1 | Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems显示文摘为内长的基因的柔韧的 transcriptional 激活的用户友好的工具高度在植物被要求。我们以前显示出那由撤销联系 CRISPR 的蛋白质组成的一个 dCas9-VP64 系统(dCas9 ) 9 与四辆双人脚踏车熔化了使活跃之物 VP16 (VP64 ) 能被用于的 transcriptional 的重复在植物的内长的基因的 transcriptional 激活。在这研究,我们在植物为提高的 transcriptional 激活开发了向量系统的第二代。我们为基于 dCas9 的 transcriptional 激活测试了多重策略,并且由 dCas9 和产出的修改指南 RNA 支架 gRNA2.0 (指明的 CRISPR-Act2.0 ) 发现那 VP64 的同时的招募更强壮的 transcriptional 激活比 dCas9-VP64 系统。而且,我们开发了复合抄写为在植物的多达四基因的同时的激活的像使活跃之物的受动器激活(mTALE 行为) 系统。我们的结果建议 mTALE 行为是比在测试的大多数情况中的 CRISPR-Act2.0 甚至更有效的。另外,我们用积极反馈环探索了织物特定的基因激活。有趣地,我们的学习比当由激活探针使不安时,其它可以引起目标基因 silencing 到 transcriptional 激活,和紧调整的基因的表明某些内长的基因是更顺从的。因此,这些新工具能被用来调查基因规章的网络和他们的控制机制。复合 CRISPR-Act2.0 和 mTALE 行为系统的汇编是两个基于优化并且克隆策略的 PCR 独立的金门和网关,它将在 dicots 和 monocots 便于 transcriptional 激活应用程序。 | Levi G. Lowder Jianping Zhou Yingxiao Zhang Aimee Malzahn Zhaohui Zhong Tzung-Fu Hsieh Daniel F. Voytas Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，2： | 19 |
| 2 | A review focused on the psy- chological effectiveness of Tai Chi on different populations显示文摘 | Zhang L Layne C Lowder T | 2012 | Evid Based Complement Alternat Med2012，，： | 1 |
| 3 | Recent Humanto-Poultry Host Jump,Adaptation,and Pandemic Spread of Staphylococcus Aureus显示文摘 | LOWDER B V GUINANE C M BEN ZAKOUR NL | 2009 | Proc Natl Acad Sci U S A2009，106，19： | 1 |
| 4 | Possterior scleritis with annular ciliochoroidal detachment 显示文摘 | Dodda E M Lowder C Y Barnhorst D A | 1995 | Am J Ophthalmol1995，120，5： | 1 |
| 5 | A randomized, controlled trial to compare ketotolac tromethamine versus pla- cebo after cesarean section to ruduce pain and narcotic usage 显示文摘 | Lowder JL Shackelford DP Holbert D | 2003 | Alia J Obstet Gynecol2003，189，6： | 1 |
| 6 | Episiotomy in the United States: has anything changed?显示文摘 | Elizabeth A. Frankman Li Wang Clareann H. Bunker Jerry L. Lowder | 2009 | American Journal of Obstetrics and Gynecology2009，，5： | 1 |
| 7 | The role of apical vaginal support in the appearance of anterior and posterior vaginal pro lapse显示文摘 | Lowder JL Park A J Ellison R | 2008 | Obstet Gynecol2008，111，： | 1 |
| 8 | Lower reproductive tract fistula repairs in inpatient US women, 1979–2006显示文摘 | Heidi Brown Li Wang Clareann Bunker Jerry Lowder | 2012 | International Urogynecology Journal2012，，4： | 1 |
| 9 | A phase II multi- center study of visilizumab, humanized anti-CD3 antibody, to treat steroid-refractory acute graft-versus-host disease 显示文摘 | Carpenter P A Lowder J Johnston L | 2005 | Biol Blood Marrow Transplant2005，11，6： | 1 |
| 10 | Sarcoidosis显示文摘 | Margolis R Lowder CY | 2007 | Curr Opin Ophthalmol2007，18，： | 1 |
| 11 | Lymphocyte subpopulations in healthy 1 - 3 - day - old infants 显示文摘 | O' Gorman MR Millard DD Lowder JN | 1998 | Cytometry1998，15，34： | 1 |
| 12 | SSJD14101300027181显示文摘 | Breno Rocha Lima Francesco Pichi Careen Y. Lowder | 2014 | International Ophthalmology2014，，5： | 1 |
| 13 | Retinal and choroidal vascular occlusion after posterior sub=tenon friamcinolone injection显示文摘 | LOWDER C Y ROTH D B | 2002 | Am J Ophthalmol2002，134，1： | 1 |
| 14 | Posterior scleritis with annular ciliochoroidal detachment显示文摘 | Dodds EM Lowder CY Barnhorst DA Lavertu P Caravella LP White DE | 1995 | Am JOphthalmol1995，120，： | 1 |
| 15 | Early treatment response of fluocinolone (retisert) implantation in patients with uveitic macular edema: an optical coherence tomography study显示文摘 | Shen BY Punjabi OS Lowder CY | 2013 | Retina2013，33，4： | 1 |
| 16 | Vitrectomy for the Diagnosis and Management of Uveitis of Unknown Cause显示文摘 | Ron Margolis Oswaldo F.M. Brasil Careen Y. Lowder Rishi P. Singh Peter K. Kaiser Scott D. Smith Victor L. Perez Christine Sonnie Jonathan E. Sears | 2007 | Ophthalmology2007，，10： | 1 |
| 17 | Prophylactic antibiotics after urodynamics in women:a decision analysis显示文摘 | Lowder JL Burrows LJ Howden NL | 2007 | Int Urogynecol J Pelvic Floor Dysfunct2007，18，2： | 1 |
| 18 | Compelling evidence that a single nucleotide substitution in TYRP1 is responsible for coat-colour polymorphism in a free-living population of Soay sheep显示文摘 | Gratten J Beraldi D Lowder B V | 2007 | Proceed- ings A of the Royal Society2007，274，： | 1 |
| 19 | Cardiovascular exer- cise training extends influenza vaccine seroprotection in sed- entary older adults: the immune function intervention trial显示文摘 | Woods JA Keylock KT Lowder T | 2009 | J Am Geriatr Soc2009，57，： | 1 |
| 20 | Glaucoma in patients withocular inflammatory disease显示文摘 | Kuchtey RW Lowder CY Smith SD | 2005 | Ophthalmol Clin North Am2005，18，3： | 1 |
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