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| 1 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 2 | Outcome of cytomegalovirus infections in patients with inflammatory bowel disease显示文摘 | Konstantinos A Papadakis Jim K Tung Scott W Binder Lori Y Kam Maria T Abreu Stephan R Targan Eric A Vasiliauskas | 2001 | The American Journal of Gastroenterology2001，，7： | 2 |
| 3 | Improved endpoint detector for isolated word recognition显示文摘 | Lori F Lamel Lawrence R Rabiner Aaron E Rosenberg | | 0，，04： | 1 |
| 4 | Manufacturing and service supply chain perfor- mance: a comparative analysis显示文摘 | Kaushik Sengupta Daniel R Heiser Lori S Cook | 2006 | The Journal of Supply Chain Management2006，42，4： | 1 |
| 5 | Modelling in data base management systems显示文摘 | GRAY J N LORIE R A PUTZOLU G R | 1976 | Chapter Granularity of locks and degrees of consistency in a shared data base1976，，： | 1 |
| 6 | Electronic Medical Record Metadata:Uses and Liability显示文摘 | Thomas R McLean Lori Burton Chris C Haller | 2008 | J Am Coll Surg2008，206，3： | 1 |
| 7 | Exploiting Database Parallelism in A Message- passing Multiprocessor 显示文摘 | Lorie R A Daudenarde J Stamos J W | 1991 | IBM Journal of Research and Development1991，35，56： | 1 |
| 8 | Current and emerging quantitative magnetic resonance imaging methods forassessing and predicting the response of breast cancer to neo- adjuvant therapy显示文摘 | Richard G Abramson Lori R Arlinghaus | 2012 | Breast Cancer (London)2012，2012，4： | 1 |
| 9 | The bone-cartilage unit in os- teoarthritis显示文摘 | LORIES R J LUYTEN F P | 2011 | Nat Rev Rheumatol2011，7，1： | 1 |
| 10 | General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold显示文摘 | Vincke C Loris R Saerens D etal | 2009 | J Biol Chem2009，284，： | 1 |
| 11 | GDF5 deficiency in mice is associated with instability-driven joint damage, gait and subehondral bone exhanges显示文摘 | Daans M Luyten F P Lories R J | 2011 | Ann Rheum Dis2011，70，1： | 1 |
| 12 | Freeman,Cystoventricular Shunting of Intracranial Arachnoid Cysts显示文摘 | Lori A Bride M Ken R | 2003 | Pediatr neurosurg2003，39，6： | 1 |
| 13 | Glucoraphasatin and glucoraphenin, a redox pair of glucosinolates of brassicaceae, differently affect metabolizing enzymes in rats显示文摘 | BARILLARI J LORI R BROCCOLI M | 2007 | Journal of Agricultural and Food Chemistry2007，55，14： | 1 |
| 14 | General strategy to humanize a camelid single-domain antibody and iden- tification of a universal humanized nanobody scaffold 显示文摘 | Vincke C Loris R Saerens D | 2009 | The Journal of Biological Chemistry2009，284，5： | 1 |
| 15 | Identification of a universal VHH framework to graft non-canonical antigen- binding loops of camel single-domain antibodies 显示文摘 | Saerens D Pellis M Loris R | 2005 | Journal of Molecular Biology2005，352，3： | 1 |
| 16 | Stability-indicating chromatographic determination of hydroquinone in combination with tretinoin and fluocinolone acetonide in pharmaceutical formulations with a photodegradation kinetic study显示文摘 | Abbas S S Elghobashy M R Lories I | 2015 | RSC Advances2015，5，43： | 1 |
| 17 | Glucoraphasatin and glucoraphenin, a redox pair of glucosinolates of Brassicaceae, differently affect metabolizing enzymes in rats显示文摘 | BARILLARI J LORI R BROCCOLI M | 2007 | Journal of Agricultural and Food Chemistry2007，55，44： | 1 |
| 18 | Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation显示文摘 | Tielker D Hacker S Loris R | 2005 | Microbiology2005，151，5： | 1 |
| 19 | Effects of o,p’-DDE,heptachlor,and 17β-estradiol on vitellogenin gene expression and the growth hormone/insulin-like growth factor-I axis in the tilapia,Oreochromis mossambicus显示文摘 | Lori K D Nancy V Larry G R | 2009 | Comparative Biochemistry and Physiology Part C2009，149，4： | 1 |
| 20 | Principles of structures of animal and plant lectins显示文摘 | Loris R | 2002 | Biochim Biophys Acta2002，1572，： | 1 |
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