|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A measurement-driven adaptive probability hypothesis density filter for multitarget tracking显示文摘This paper studies the dynamic estimation problem for multitarget tracking. A novel gating strategy that is based on the measurement likelihood of the target state space is proposed to improve the overall effectiveness of the probability hypothesis density(PHD) filter. Firstly, a measurement-driven mechanism based on this gating technique is designed to classify the measurements. In this mechanism, only the measurements for the existing targets are considered in the update step of the existing targets while the measurements of newborn targets are used for exploring newborn targets. Secondly, the gating strategy enables the development of a heuristic state estimation algorithm when sequential Monte Carlo(SMC) implementation of the PHD filter is investigated, where the measurements are used to drive the particle clustering within the space gate.The resulting PHD filter can achieve a more robust and accurate estimation of the existing targets by reducing the interference from clutter. Moreover, the target birth intensity can be adaptive to detect newborn targets, which is in accordance with the birth measurements. Simulation results demonstrate the computational efficiency and tracking performance of the proposed algorithm. | Si Weijian Wang Liwei Qu Zhiyu | 2015 | Chinese Journal of Aeronautics2015，28，6： | 9 |
| 2 | Aggregation-induced emission luminogen-assisted stimulated emission depletion nanoscopy for super-resolution mitochondrial visualization in live cells显示文摘Aggregation-induced emission luminogens (AIEgens) are fluorescent agents thatare ideal for bioimaging and have been widely used for organeUe targeting, cellularmapping, and tracing. Owing to their promising characteristics, AIEgen-basednanoparticles have recently been used for the stimulated emission depletion(STED) super-resolution imaging of fixed cells. In the present study, and for thefirst time, we used an AIEgen for dynamic STED nanoscopic imaging of a specificorganelle in live cancer cells. TPA-T-CyP is a synthetic red&NIR-emittingluminogen with AIE features that can spontaneously and specifically aggregateon mitochondria without the need for encapsulation or surface modification.The STED efficiency of aggregated TPA-T-CyP can reach more than 80%, andsuper-resolution imaging of TPA-T-CyP-stained mitochondria in live HeLa cellsis possible, with a lateral spatial resolution of 74 nm. We found that TPA-T-CyPenabled the dynamic visualization of mitochondria, and the motion, fusion, andfission of mitochondria were dearly observable on a super-resolution scale.AIEgen-based super-resolution organelle visualization has great potential formany basic biomedical studies. | Dongyu Li Xiang Ni Xiaoyan Zhang Liwei Liu Junle Qu Dan Ding Jun Qian | 2018 | Nano Research2018，11，11： | 4 |
| 3 | Genomic variations in the counterpart normal controls of lung squamous cell carcinomas显示文摘 | Dalin Zhang Liwei Qu Bo Zhou Guizhen Wang Guangbiao Zhou | 2018 | Frontiers of Medicine2018，12，3： | 2 |
| 4 | In vivo mice brain microcirculation monitoring based on contrast-enhanced SD-OCT显示文摘In this paper,we proposed a contrast-enhanced homemade spectral domain optical coherence tomography(SD-OCT)method for monitoring of brain microcirculation.We used the polyethylene glycol(PEG)-ylated gold nanorods(GNRs)as a contrast-enhanced agent,obtained clearly 2D and 3D OCT images of blood vessels and dynamic changes of probes in mouse blood vessels.Owing to high scattering of the PEG-GNRs,more tiny blood vessels can be imaged and the OCT signal can be enhanced by 5.87 dB after injection of PEG-GNRs for 20 min,the enhancement then declined gradually for 60 min.Our results demonstrate an effective technique for the enhanced imaging of blood vessels in vivo,especially for studies of the brain microcirculation,which could be serviced for disease mechanism research and therapeutic drug monitoring. | Shaozhuang Yang Liwei Liu Yixin Chang Ningning Zhang Kun Liu Liang Hong Bingling Chen Yue Zhao Rui Hu Junle Qu | 2019 | Journal of Innovative Optical Health Sciences2019，，1： | 2 |
| 5 | Recent advances in nonlinear optics for bio‐imaging applications显示文摘Nonlinear optics,which is a subject for studying the interaction between intense light and materials,has great impact on various research fields.Since many structures in biological tissues exhibit strong nonlinear optical effects,nonlinear optics has been widely applied in biomedical studies.Especially in the aspect of bio-imaging,nonlinear optical techniques can provide rapid,label-free and chemically specific imaging of biological samples,which enable the investigation of biological processes and analysis of samples beyond other microscopy techniques.In this review,we focus on the introduction of nonlinear optical processes and their applications in bio-imaging as well as the recent advances in this filed.Our perspective of this field is also presented. | Silu Zhang Liwei Liu Sheng Ren Zilin Li Yihua Zhao Zhigang Yang Rui Hu Junle Qu | 2020 | Opto-Electronic Advances2020，3，10： | 2 |
| 6 | Genotoxicity evaluation of drinking water sources in human peripheral blood lymphocytes using the comet assay显示文摘在到人的健康的饮用水的有机污染物的潜在的伤害广泛地集中于在世界上;有 genotoxic 物质的越来越多的污染物质被释放进水生环境。水来源样品从南京城市的 7 个不同地区被收集。从饮用水来源的器官的摘录的潜在的 genotoxicity 在人的外部淋巴细胞借助于彗星试金被调查。结果证明从所有水来源样品的所有器官的摘录能在不同层次导致人的外部血淋巴细胞的脱氧核糖核酸损坏。重要差别(P <
0.01 ) 当时,被观察与溶剂控制相比。脱氧核糖核酸损坏随原来的水来源的剂量的增加增加了。脱氧核糖核酸损坏的重要差别在不同饮用水来源被观察,在 100 ×的剂量由多重比较分析出现;脱氧核糖核酸损坏的度由 Hushu 供水系统对待(在镇水平) 是最严肃的,任意的单位(AU ) 然而,是 141.62 ± 6 .96 Shangyuanmen 供水系统的(在城市水平) 仅仅是 109.64 ± 2 .97。分析也表明镇的水来源的 genotoxicity 比城市的高。结果证明彗星试金能成功地被用于监视饮用水来源的节目的 genotoxicity。 | WU Yulin CHEN Haigang LI Zhaoli SUN Liwei QU Mengmeng LI Mei KONG Zhiming | 2008 | Journal of Environmental Sciences2008，20，4： | 2 |
| 7 | Bis[4‐(4‐maleimidephen‐oxy)phenyl]propane/ N , N ‐4,4′‐bismaleimidodiphenylmethyene blend modified with diallyl bisphenol A显示文摘 | Chunyan Qu Liwei Zhao Dezhi Wang Hongfeng Li Wanbao Xiao Cheng’e Yue | 2014 | J Appl Polym Sci2014，，12： | 1 |
| 8 | Monitoring microenvironment of Hep G2 cell apoptosis using two-photon fluorescence lifetime imaging microscopy显示文摘Apoptosis is very important for the maintenance of cellular homeostasis and is closely related to the occurrence and treatment of many diseases.Mitochondria in cells play a crucial role in programmed cell death and redox processes.Nicotinamide adenine dinucleotide(NAD(P)H)is the primary producer of energy in mitochondria,changing NAD(P)H can directly reflect the physiological state of mitochondria.Therefore,NAD(P)H can be used to evaluate metabolic response.In this paper,we propose a noninvasive detection method that uses two-photon fluorescence lifetime imaging microscopy(TP-FLIM)to characterize apoptosis by observing the binding kinetics of cellular endogenous NAD(P)H.The result shows that the average fluorescence lifetime of NAD(P)H and the fluorescence lifetime of protein-bound NAD(P)H will be affected by the changing pH,serum content,and oxygen concentration in the cell culture environment,and by the treatment with reagents such as H2O2 and paclitaxel.Taxol(PTX).This noninvasive detection method realized the dynamic detection of cellular endogenous substances and the assessment of apoptosis. | Kexin Wang Shiyao Tang Shiqi Wang Fangrui Lin Gengjin Zou Junle Qu Liwei Liu | 2022 | Journal of Innovative Optical Health Sciences2022，15，3： | 1 |
| 9 | Impact of living donor liver with steatosis and idiopathic portal inflammation on clinical outcomes in pediatric liver transplantation:Beijing experience显示文摘Background:To evaluate the impact of steatosis and/or idiopathic portal inflammation(IPI)in living donor livers on recipients’clinical outcomes.Methods:We assessed 305 qualified donor liver samples from June 2013 to December 2018.Donors and recipients’clinical characteristics,including follow-up data were retrieved.The graft and overall survival with/without steatosis or portal inflammation were compared by Kaplan-Meier analysis.Results:For living donors,the medium age of was 31.2(28,35.8)years old;liver histopathology showed macrovesicular steatosis:0-5%264/305(86.6%)and 5-30%41/305(13.4%),IPI:no 220/305(72.1%)and mild 85/305(27.9%).For recipients,the medium age was 1.0(0.6,1.5)years old;the median pediatric-end-stage-liver-disease score was 16(5.0,26.0)and medium follow-up time was 32.8(24.8,52.0)months.Biliary atresia(69.5%)was the main indication for liver transplantation(LT).Conclusions:The presence of steatosis and portal inflammation of the donor liver did not impact the clinical outcomes including transaminase or bilirubin normalization,short-/long-term complications and recipients’survival.However,recipients with high pediatric-end-stage-liver-disease score(>16)receiving donor liver with portal inflammation,but not steatosis,had trend negative effect on recipients’survival.In conclusion,donor livers with mild steatosis and portal inflammation were qualified for pediatric living donor LT.However,donor liver with mild portal inflammation would better not be allocated to recipients with high pediatric-end-stage-liver-disease score.This study provided new evidence in pediatric living donor liver allocation. | Xinyan Zhao Yafei He Jimin Liu Qian Zhang Liwei Liu Wei Qu Ying Liu Zhigui Zeng Haiming Zhang Jidong Jia Liying Sun Lin Wei Zhijun Zhu | 2022 | Hepatobiliary Surgery and Nutrition2022，11，3： | 1 |
| 10 | Deep learning model improves radiologists'performance in detection and classification of breast lesions显示文摘Objective:Computer-aided diagnosis using deep learning algorithms has been initially applied in the field of mammography,but there is no large-scale clinical application.Methods:This study proposed to develop and verify an artificial intelligence model based on mammography.Firstly,mammograms retrospectively collected from six centers were randomized to a training dataset and a validation dataset for establishing the model.Secondly,the model was tested by comparing 12 radiologists’performance with and without it.Finally,prospectively enrolled women with mammograms from six centers were diagnosed by radiologists with the model.The detection and diagnostic capabilities were evaluated using the freeresponse receiver operating characteristic(FROC)curve and ROC curve.Results:The sensitivity of model for detecting lesions after matching was 0.908 for false positive rate of 0.25 in unilateral images.The area under ROC curve(AUC)to distinguish the benign lesions from malignant lesions was0.855[95%confidence interval(95%CI):0.830,0.880].The performance of 12 radiologists with the model was higher than that of radiologists alone(AUC:0.852 vs.0.805,P=0.005).The mean reading time of with the model was shorter than that of reading alone(80.18 s vs.62.28 s,P=0.032).In prospective application,the sensitivity of detection reached 0.887 at false positive rate of 0.25;the AUC of radiologists with the model was 0.983(95%CI:0.978,0.988),with sensitivity,specificity,positive predictive value(PPV),and negative predictive value(NPV)of94.36%,98.07%,87.76%,and 99.09%,respectively.Conclusions:The artificial intelligence model exhibits high accuracy for detecting and diagnosing breast lesions,improves diagnostic accuracy and saves time. | Yingshi Sun Yuhong Qu Dong Wang Yi Li Lin Ye Jingbo Du Bing Xu Baoqing Li Xiaoting Li Kexin Zhang Yanjie Shi Ruijia Sun Yichuan Wang Rong Long Dengbo Chen Haijiao Li Liwei Wang Min Cao | 2021 | Chinese Journal of Cancer Research2021，33，6： | 1 |
| 11 | Insight on metal ions inducing chiral self-assembly of DNA in silica mineralization显示文摘The self-assembly of DNA provides an attractive approach to understanding structural formation mechanism in living organisms and to assisting applications in materials chemistry.Herein,we investigated the effect of metal ions on chiral self-assembly of DNA through the synthesis of chiral mesostructured silica via self-assembly of metal ions,DNA,and silica source.31 types of multivalent cationic metal ions were found to induce formation of chiral impeller-like DNA-silica complexes due to the chiral stacking of DNA.The strength of the interaction between the metal ion and phosphate group of DNA was speculated for the chiral stacking of DNA due to close distance of adjacent DNA to assure mutual recognition.Theoretical calculations indicated that chiral packing of DNA depends on the stability of the bridging phosphate-metal ion-phosphate bonds of DNA based on electron delocalization in d-orbital conjugation of metal ions. | Arepati Azhati Lu Han Zhibei Qu Zhouhong Ren Xi Liu Liwei Chen Shunai Che | 2023 | Nano Research2023，16，3： | 0 |
| 12 | High frequency of alternative splicing variants of the oncogene Focal Adhesion Kinase in neuroendocrine tumors of the pancreas and breast显示文摘The characteristic genetic abnormality of neuroendocrine neoplasms(NENs),a heterogeneous group of tumors found in various organs,remains to be identified.Here,based on the analysis of the splicing variants of an oncogene Focal Adhesion Kinase(FAK)in The Cancer Genome Atlas datasets that contain 9193 patients of 33 cancer subtypes,we found that Box 6/Box 7-containing FAK variants(FAK^(6/7))were observed in 7(87.5%)of 8 pancreatic neuroendocrine carcinomas and 20(11.76%)of 170 pancreatic ductal adenocarcinomas(PDACs).We tested FAK variants in 157 tumor samples collected from Chinese patients with pancreatic tumors,and found that FAK^(6/7)was positive in 34(75.6%)of 45 pancreatic NENs,19(47.5%)of 40 pancreatic solid pseudopapillary neoplasms,and 2(2.9%)of 69 PDACs.We further tested FAK splicing variants in breast neuroendocrine carcinoma(BrNECs),and found that FAK^(6/7)was positive in 14(93.3%)of 15 BrNECs but 0 in 23 non-NEC breast cancers.We explored the underlying mechanisms and found that a splicing factor serine/arginine repetitive matrix protein 4(SRRM4)was overexpressed in FAK^(6/7)-positive pancreatic tumors and breast tumors,which promoted the formation of FAK^(6/7)in cells.These results suggested that FAK^(6/7)could be a biomarker of NENs and represent a potential therapeutic target for these orphan diseases. | Dawei Xie Zheng Wang Beibei Sun Liwei Qu Musheng Zeng Lin Feng Mingzhou Guo Guizhen Wang Jihui Hao Guangbiao Zhou | 2023 | Frontiers of Medicine2023，17，5： | 0 |
| 13 | Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy(FLIM)显示文摘Fluorescence Lifetime Imaging Microscopy(FLIM)is an advanced tool that enables the description of exponential decay rate distribution of fuorescent molecules in the samples.This technique has been broadly used in biomedicine,material science,chemistry,and other related research fields,due to its ability to illustrate both localization of specific fuorophores and fuorophores'local microenvironment,and it is superior to fluorescence intensity based imaging. | Junle Qu Liwei Liu | 2019 | Journal of Innovative Optical Health Sciences2019，，5： | 0 |
| 14 | Fast fluorescence lifetime imaging techniques:A review on challenge and development显示文摘Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications. | Xiongbo Liu Danying Lin Wolfgang Becker Jingjing Niu Bin Yu Liwei Liu Junle Qu | 2019 | Journal of Innovative Optical Health Sciences2019，，5： | 0 |
| 15 | Atomic-resolution characterization on the structure of strontium doped barium titanate nanoparticles显示文摘Ferroelectric barium titanate nanoparticles(BTO NPs)may play critical roles in miniaturized passive electronic devices such as multi-layered ceramic capacitors.While increasing experimental and theoretical understandings on the structure of BTO and doped BTO have been developed over the past decade,the majority of the investigation was carried out in thin-film materials;therefore,the doping effect on nanoparticles remains unclear.Especially,doping-induced local composition and structure fluctuation across single nanoparticles have yet to be unveiled.In this work,we use electron microscopy-based techniques including high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM),integrated differential phase contrast(iDPC)-STEM,and energy dispersive X-ray spectroscopy(EDX)mapping to reveal atomically resolved chemical and crystal structure of BTO and strontium doped BTO nanoparticles.Powder X-ray diffraction(PXRD)results indicate that the increasing strontium doping causes a structural transition from tetragonal to cubic phase,but the microscopic data validate substantial compositional and microstructural inhomogeneities in strontium doped BTO nanoparticles.Our work provides new insights into the structure of doped BTO NPs and will facilitate the materials design for next-generation high-density nano-dielectric devices. | Haoyu Jiang Jizhen Qi Dongchang Wu Wei Lu Jiahui Qian Haifeng Qu Yixiao Zhang Pei Liu Xi Liu Liwei Chen | 2021 | Nano Research2021，14，12： | 0 |
| 16 | Implementation and application of FRET-FLIM technology显示文摘With the development of the new detection methods and the function of fluorescent molecule,researchers hope to further explore the internal mechanisms of organisms,monitor changes in the intracellular microenvironment,and dynamic processes of molecular interactions in cells.Fluo-rescence resonance energy transfer(FRET)describes the energy transfer process between donor fluorescent molecules and acceptor fluorescent molecules.It is an important means to detect protein-protein interactions and protein conformation changes in cells.Fluorescence lifetime imaging microscopy(FLIM)enables noninvasive measurement of the fAuorescence lifetime of fluorescent particles in vivo.The FRET-FLIM technology,which is use FLIM to quantify and analyze FRET,enables real-time monitoring of dynamic changes of proteins in biological cells and analysis of protein interaction mechanisms.The distance between donor and acceptor and their respective fAuorescent lifetime,which are of great importance for studying the mechanism of intracellular activity can be obtained by data analysis and algorithm ftting. | Shiqi Wang Binglin Shen Sheng Ren Yihua Zhao Silu Zhang Junle Qu Liwei Liu | 2019 | Journal of Innovative Optical Health Sciences2019，，5： | 0 |
| 17 | A three-dimensional hollow graphene fiber microelectrode with shrink-effect-enabled enzyme immobilization for sensor applications显示文摘Microelectrodes are a type of electrode with microscopic dimension, and due to their rapid diffusing rates, high electrical current densities and signal-to-noise ratios, they can help improve the detection sensitivity for various analytes [1]. Investigations on the fabrication and the application of microelectrodes are highly relevant. Fiber-type microelectrodes with one-dimensional (ID) microstructures that possess ID diffusion, particularly carbon fiber microelectrodes, have been widely fabricated and used in many different types of sensors due to their low cost, small volume, portability, and good biocompatibility [1,2]. However, poor electrocatalytic activity and low response currents preclude the applications of carbon fiber microelectrodes in sensors. | Liwei Chen Xiaoteng Ding Jinfeng Zeng Lejiao Chongbei Wu Yuze Wang Qing Han Liangti Qu | 2019 | Science Bulletin2019，64，11： | 0 |
| 18 | Deep learning autofluorescence-harmonic microscopy显示文摘Laser scanning microscopy has inherent tradeoffs between imaging speed,field of view(FOV),and spatial resolution due to the limitations of sophisticated mechanical and optical setups,and deep learning networks have emerged to overcome these limitations without changing the system.Here,we demonstrate deep learning autofluorescence-harmonic microscopy(DLAM)based on self-alignment attention-guided residual-in-residual dense generative adversarial networks to close the gap between speed,FOV;and quality.Using the framework,we demonstrate label-free large-field multimodal imaging of clinicopathological tissues with enhanced spatial resolution and running time advantages.Statistical quality assessments show that the attention-guided residual dense conneaions minimize the persistent noise,distortions,and scanning fringes that degrade the autofluorescence-harmonic images and avoid reconstruction artifaas in the output images.With the advantages of high contrast,high fidelity,and high speed in image reconstruction,DLAM can act as a powerful tool for the noninvasive evaluation of diseases,neural activity,and embryogenesis. | Binglin Shen Shaowen Liu Yanping Li Ying Pan Yuan Lu Rui Hu Junle Qu Liwei Liu | 2022 | Light(Science & Applications)2022，11，4： | 0 |
| 19 | Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei显示文摘The filamentous fungus Trichoderma reesei is widely used for the production of lignocellulolytic enzymes in industry.XYR1 is the major transcriptional activator of cellulases and hemicellulases in T.reesei.However,rational engineering of XYR1 for improved lignocellulolytic enzymes production has been limited by the lack of structure information.Here,alanine 873 was identified as a new potential target for the engineering of XYR1 based on its structure predicted by AlphaFold2.The mutation of this residue to tyrosine enabled significantly enhanced production of xylanolytic enzymes in the medium with cellulose as the carbon source.Moreover,xylanase and cellulase production increased by 56.7-and 3.3-fold,respectively,when glucose was used as the sole carbon source.Under both conditions,the improvements of lignocellulolytic enzyme production were higher than those in the previously reported V821F mutant.With the enriched hemicellulases and cellulases,the crude enzymes secreted by the A873Y mutant strain produced 51%more glucose and 52%more xylose from pretreated corn stover than those of the parent strain.The results provide a novel strategy for engineering the lignocellulolytic enzyme-producing capacity of T.reesei,and would be helpful for understanding the molecular mechanisms of XYR1 regulation. | Qinqin Zhao Zezheng Yang Ziyang Xiao Zheng Zhang Jing Xing Huiqi Liang Liwei Gao Jian Zhao Yinbo Qu Guodong Liu | 2023 | Synthetic and Systems Biotechnology2023，8，4： | 0 |
| 20 | Halogen-doped phosphorescent carbon dots for grayscale patterning显示文摘Flexible organic materials that exhibit dynamic ultralong room temperature phosphorescence(DURTP)via photoactivation have attracted increasing research interest for their fascinating functions of reversibly writing-reading-erasing graphic information in the form of a long afterglow.However,due to the existence of a nonnegligible activation threshold for the initial exposure dose,the display mode of these materials has thus far been limited to binary patterns.By resorting to halogen element doping of carbon dots(CDs)to enhance intersystem crossing and reduce the activation threshold,we were able to produce,for the first time,a transparent,flexible,and fully programmable DURTP composite film with a reliable grayscale display capacity.Examples of promising applications in UV photography and highly confidential steganography were constructed,partially demonstrating the broad future applications of this material as a programmable platform with a high optical information density. | Yanfeng Liu Mahmoud Al-salihi Yong Guo Roman Ziniuk Songtao Cai Luwei Wang Yuan Li Zhigang Yang Dengfeng Peng Kai Xi Zhongfu An Xudong Jia Liwei Liu Wei Yan Junle Qu | 2022 | Light(Science & Applications)2022，11，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员