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| 1 | Effects of electroacupuncture at Zusanli (ST 36) on neurons in the colonic myenteric plexus in rats with irritable bowel syndrome with constipation显示文摘We studied the effects of electroacupuncture at Zusanli (ST 36) on neurons in the colonic myenteric plexus and on defecation in rats with irritable bowel syndrome with constipation (IBS-C). We also used intragastric administration of pinaverium bromide as a positive control treatment to reveal the pathway mediating the onset of IBS-C. Both electroacupuncture and pinaverium bromide greatly improved defecation in rats with IBS-C. Immunohistochemical staining of the enteric nervous system neuronal marker protein gene product 9.5 in the colonic myenteric plexus showed that electroacupuncture by itself, or in combination with pinaverium bromide, increased the number of neurons and the staining intensity of protein gene product 9.5 in the colonic myenteric plexus. We conclude that visceral hypersensitivity is likely to be a primary cause of constipation in IBS-C rats. | Liting Zhu Zhonghan Li Bin Xu Chen Xia Jie Cheng Xiaoren Xiang Yi Zhu | 2011 | Neural Regeneration Research2011，6，33： | 3 |
| 2 | Single-cell transcriptomes of peripheral blood cells indicate and elucidate severity of COVID-19显示文摘The blood and immune system of coronavirus disease 2019(COVID-19)infected patients are dysfunctional,and numerous studies have been conducted to resolve their characteristics and pathogenic mechanisms.Nevertheless,the variations of immune responses along with disease severity have not been comprehensively documented.Here,we profiled the single-cell transcriptomes of 96,313 peripheral blood mononuclear cells(PBMCs)derived from 12 COVID-19 patients(including four moderate,four severe and four critical cases)and three healthy donors.We showed that proliferative CD8 effector T cells with declined immune functions and cytotoxicity accumulated in the critical stage.By contrast,the quantity of natural killer(NK)cells was significantly reduced,while they exhibited enhanced immune activities.Notably,a gradually attenuated responseto COVID-19 along with disease severity was observed in monocytes,in terms of cellular composition,transcriptional discrepancy and transcription factor regulatory network.Furthermore,we identified immune cell-type dependent cytokine signatures distinguishing the severity of COVID-19 patients.In addition,cell interactions between CD8 effector T/NK cells and monocytes mediated by inflammatory cytokines were enhanced in moderate and severe stages,but weakened in critical cases.Collectively,our work uncovers the cellular and molecular players underlying the disordered and heterogeneous immune responses associated with COVID-19 severity,which could provide valuable insights for the treatment of critical COVID-19 patients. | Xiaowei Xie Xuelian Cheng Gaoxiang Wang Biao Zhang Mengyao Liu Liting Chen Hui Cheng Sha Hao Jianfeng Zhou Ping Zhu Tao Cheng | 2021 | Science China(Life Sciences)2021，64，10： | 3 |
| 3 | Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections显示文摘As a typical human pathogenic fungus,Cryptococcus neoformans is a life-threatening invasive fungal pathogen with a worldwide distribution causing w700,000 deaths annually.Cryptococcosis is not just an infection with multi-organ involvement,intracellular survival and extracellular multiplication of the fungus also play important roles in the pathogenesis of C.neoformans infections.Because adequate accumulation of drugs at target organs and cells is still difficult to achieve,an effective delivery strategy is desperately required to treat these infections.Here,we report a bioresponsive micro-to-nano(MTN)system that effectively clears the C.neoformans in vivo.This strategy is based on our in-depth study of the overexpression of matrix metalloproteinase 3(MMP-3)in infectious microenvironments(IMEs)and secreted protein acidic and rich in cysteine(SPARC)in several associated target cells.In this MTN system,bovine serum albumin(BSA,a natural ligand of SPARC)was used for the preparation of nanoparticles(NPs),and then microspheres were constructed by conjugation with a special linker,which mainly consisted of a BSA-binding peptide and an MMP-3-responsive peptide.This MTN system was mechanically captured by the smallest capillaries of the lungs after intravenous injection,and then hydrolyzed into BSA NPs by MMP-3 in the IMEs.The NPs further targeted the lung tissue,brain and infected macrophages based on the overexpression of SPARC,reaching multiple targets and achieving efficient treatment.We have developed a size-tunable strategy where microspheres'shrink'to NPs in IMEs,which effectively combines active and passive targeting and may be especially powerful in the fight against complex fungal infections. | Liting Cheng Miao-Miao Niu Tong Yana Zhongyi Ma Kexin Huang Ling Yang Xin Zhong Chong Li | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 2 |
| 4 | Purification and characterization of a thermostable cellulase-free xylanase from the newly isolated Paecilomyces themophila显示文摘 | LI Lite TIAN Hongmei CHENG Yongqiang | 2006 | Enzyme and Microbial Technology2006，38，： | 1 |
| 5 | Effect of the Maillard Reaction on Angiotensin I-Converting Enzyme (ACE)-Inhibitory Activity of Douchi During Fermentation显示文摘 | Hui Wang Yongyu Li Yongqiang Cheng Lijun Yin Lite Li | 2013 | Food and Bioprocess Technology2013，，1： | 1 |
| 6 | Vasorelaxant effect of osthole on isolated thoracic aortic rings in rats显示文摘OBJECTIVE: To investigate the effect of osthole on isolated thoracic aortic rings, and to determine the potential mechanism of action.METHODS: Thoracic aortas were isolated from Wistar rats, and were suspended in tissue organ chambers for vascular tension measurement. The effect of cumulative osthole(10-9, 10-8, 10-7, 10-6, and 10-5 mol/L) on endothelium-intact and endothelium-denuded thoracic aortic rings pre-contracted with phenylephrine(PE, 10-6 mol/L) or KCl(6 × 10-2 mol/L) was recorded. Histomorphological changes of thoracic aorta were analyzed by hematoxylin-eosin. The effects of different osthole concentrations on endothelium-intact aortic rings, which were pre-inhibited with the non-selective nitric oxide synthase inhibitor L-Arg(NO2)-OMe·HCl(3 × 10-4 mol/L), endothelium-derived nitric oxide synthase inhibitor Nω-nitro-L-arginine(3 × 10-4 mol/L), guanylate cyclase inhibitor 1 H-[1,2,4] oxadiazolo [4,3-α]quinoxaline-1-one(10-5 mol/L), cyclooxygenase inhibitor indometacin(10-5 mol/L), and the Ca2+-activated potassium channel inhibitor tetraethylammonium nitrate(10-5 mol/L), and then contracted with PE, were examined. Aortic rings incubated with osthole(10-5 mol/L), phentolamine(10-5 mol/L), or verapamil(10-5 mol/L) in Ca2+-free Krebs-Henseleit solution(KHS) were stimulated with PE or KCl.RESULTS: There was a dose-dependent increase in vasorelaxation of isolated thoracic aortic rings(both with and without endothelium) with increasing osthole concentration. Hematoxylin-eosin staining showed that osthole significantly improved thoracic aorta ring morphology. Compared with the control group, there were also significant differences after incubation with L-Arg(NO2)-OMe ω-nitro-L-arginine, and 1 H-[1,2,4] oxadiazo·lo HCl,N [4,3-α] quinoxaline-1-one(P < 0.05 for all). The relaxation rate of the rings in the osthole group incubated with indometacin and tetraethylammonium nitrate were similar to controls. In Ca2+-free KHS, the PE-induced contraction was similar between the osthole(4.37% ± 0.41%) and control(4.21% ± 1.33%)groups. However, after cumulative CaCl2(0.5, 1, 1.5,2, 2.5, and 3 mmol/L), the Ca2+-induced contraction was significantly inhibited in the osthole and phentolamine groups compared with controls(P < 0.05).After cumulative CaCl2 was added to Ca2+-free KHS(high K+ concentration), the contraction rate was significantly higher than both of the control and the osthole groups(P < 0.05). The contraction rate in the osthole group was higher than the verapamil group(P < 0.05).CONCLUSION: Osthole has a vasorelaxant effect on isolated rat thoracic aortic rings, via inhibition of both receptor-operated and voltage-dependent Ca2+channels in arterial smooth muscle, leading to decreased Ca2+ influx, and via inhibition of nitric oxide release on arterial endothelial cells. | Huang Ying Kong Liting Wang Yanping Sun Mingjie Cui Haifeng Zhai Huaqiang LüCheng Fan Danping | 2019 | Journal of Traditional Chinese Medicine2019，39，4： | 1 |
| 7 | High-resolution crustal velocity structure in the Shanxi rift zone and its tectonic implications显示文摘The Shanxi rift zone,located in the Trans-North China Orogen(TNCO)of the North China Craton(NCC),is wellknown for hosting large intraplate earthquakes in continental China.The TNCO is a suture zone formed by the amalgamation of the eastern and the western blocks of the NCC.After its formation,it was reactived and deformed by later tectonic activities,which result in complex lithospheric heterogeneities.Thus,the detailed crustal structure of the Shanxi rift zone is critical for understanding the tectonics and seismogenic mechanism in this area,which will shed new lights on the formation and dynamic evolution of the NCC.In this study,we applied ambient noise tomography based on 18 months continuous records from 108 seismic stations located in Shanxi and its surroundings,in order to constrain its detailed crustal structure.We measured 4437 Rayleigh wave phase velocity dispersion curves in the period of 5–45 s from the cross-correlation functions.Next,a surface wave direct inversion algorithm based on surface-wave ray tracing was used to resolve a 3-D S-wave velocity model in the upper 60 km with lateral resolution of~50–80 km.The tomographic images show that the sedimentary thickness of the Taiyuan Basin is less than 5 km.At depth of 0–10 km,we observe a good correlation between the imaged structural variations with geological and topographic features at the surface.For example,the center of rift shows low-velocity anomalies and the uplifting areas on both sides are characterized by high velocity anomalies.The western and eastern boundaries of the slow materials coincide with the faults that control the basin.The slow material extends from the shallow surface to depth of about 15 km but it getting smaller in shape at deeper depth.For the Taiyuan Basin,Linfen Basin,and Yuncheng Basin in the central and southern parts,the structure is dominant by slow materials in the upper crust but changes to strong high-velocity anomalies in the lower crust and the uppermost mantle at depth deeper than 25 km.We interprete these high-velocity anomalies to be associated with the cold remnant of the underplated basalt in the lower crust that were formed in early Tertiary before the basin was stretched.We also observe the low-velocity anomaly beneath the Datong volcanic area,which extends from the uppermost mantle to a depth of 20 km vertically and migrates from west to east laterally.It may reflect the upwelling channel of the magmatic material in Datong.Moreover,the strong low-velocity anomalies presented north of 38°N could be related to the heated crustal materials with paritial melting as a result of the intensive magmatic activities of the Datong Volcano since the Cenozoic.In our study region,seismicity mainly concentrates in the depth range of 5–20 km and we find that most earthquakes appear to occur in places where velocity changes from high to low rapidly,with slight higher concentration in the faster material areas.In summary,our high-resolution 3-D crustal velocity model provides important seismological constraints to understand the tectonic evolution and seismicity across the Shanxi rift zone. | Liting DOU Huajian YAO Lihua FANG Song LUO Meiqin SONG Xiaomei YAN Cheng CHENG | 2021 | Science China Earth Sciences2021，64，5： | 1 |
| 8 | Photodynamic Therapy Activities of 10-(4-Formylphenyl)-5,15-bis(pentafluorophenyl)corrole and Its Gallium Complex显示文摘10-(4-Formylphenyl )-5,15-bis(pentafluorophenyl)corrole 1 并且它的镓建筑群 1-Ga 被用来在肝癌症(BEL-7402 ) 调查他们的 photocytotoxicity,肺癌症(A549 ) 和颈的癌症(Siha, Hela ) 房间线。在所有测试肿瘤房间之中, Siha 肿瘤房间对光力学的治疗(太平洋夏季时间) 最敏感在 1 的治疗和 1-Ga。1 的太平洋夏季时间 IC 50 和向 Siha 肿瘤房间的 1-Ga 分别地到达 1.3 和 0.8 µmol/L,它比 cisplatin 的高是 10 褶层。1 并且没有搬运人蛋白质的帮助, 1-Ga 可能顺利越过肿瘤房间膜过去,并且在肿瘤的细胞质主要局部性房间。在太平洋夏季时间治疗以后,增加的细胞内部的反应的氧种类(ROS ) 水平和 mitochondrial 膜潜力显著地减少了,它将最后导致肿瘤房间的 apoptosis。 | Fan Cheng Liting Huang Huahua Wang Yunjun Liu Jaipal Kandhadi Hui Wang Liangnian Ji Haiyang Liu | 2017 | Chinese Journal of Chemistry2017，35，1： | 1 |
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