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| 1 | Implications from protein uptake kinetics onto dextran-grafted Sepharose FF coupled with ion exchange and affinity ligands显示文摘Our previous studies have reported the presence of 'chain delivery' effects of protein adsorption onto ion exchangers with polymer-grafted ion-exchange groups, such as dextran-grafted and poly(ethylenimine)-modified Sepharose gels. However, it is unclear if the 'chain delivery' occurs on affinity adsorption with specific interactions. This work is designed to address this issue. A dextran-grafted Sepharose gel was prepared, and then the matrix was modified using diethylaminoethyl, a typical ion-exchange group, or octapeptide(FYCHWQDE), an affinity ligand for human immunoglobulin G(h Ig G) to prepare ion-exchange or affinity adsorbents, respectively.Results of h Ig G adsorption showed that the uptake rate represented by the effective diffusivity of h Ig G onto the dextran-grafted ion exchangers was obviously enhanced by the dextran grafting, indicating the presence of'chain delivery' of the bound proteins on the charged groups on the dextran chains. By contrast, the effective diffusivity of h Ig G changed little as ligand density increased on the dextran-grafted FYCHWQDE adsorbents.Their adsorption capacities decreased and effective diffusivities were not accelerated by the dextran grafting.Thus, this work clarified that grafted dextran could not accelerate h Ig G uptake rate on the affinity resins, or in other words, chain delivery did not occur on the specific interaction-based affinity adsorption. | Aiying Xue Linling Yu Yan Sun | 2017 | Chinese Journal of Chemical Engineering2017，25，7： | 10 |
| 2 | Protein adsorption onto diethylaminoethyl dextran modified anion exchanger:Effect of ionic strength and column behavior显示文摘Our previous studies on bovine serum albumin(BSA) adsorption to diethylaminoethyl dextran(DEAE dextran,DexD, grafting-ligand) and DEAE(D, surface-ligand) modified Sepharose FF resins found that all the grafted resins(FF-DexD and FF-D-DexD) exhibited extremely fast uptake rate(effective diffusivity, D_e, D_e/D_O> 1.4),which was six times greater than the ungrafted resins(D_e/D_O < 0.3). In this work, the influence of ionic strength(IS) on 6 typical DEAE dextran-grafted resins was investigated. Bath adsorption equilibria and kinetics, breakthrough, and linear gradient elution experiments were conducted. Commercial DEAE Sepharose FF was used for comparison. It is found that protein adsorption capacities on DEAE dextran-FF resins and the commercial resin decreased with increasing IS, but DEAE dextran-FF resins exhibited much higher capacity sensitivity to salt concentration. Besides, steeper decrease of adsorption capacities could be obtained at higher graftingligand or surface-ligand density. It is worth noting that the facilitating role of surface-ligand to the 'chain delivery' effect was weakened after adding salt, leading to the less improvement in uptake rate by increasing surface-ligand density at higher IS. Although the uptake rates of the DEAE dextran-FF resins increased first and then decreased with increasing IS, they kept the extremely high level of De values(D_e/D_O > 1.1) at the their working/binding IS range. Moreover, the DEAE dextran-FF resin displayed much higher adsorption capacities and De values than commercial ungrafted resin in their working condition. Furthermore, the column results of DEAE dextran-FF resins presented higher dynamic binding capacities than and similar elution ISs with DEAE Sepharose FF to achieve similar(or even higher) recoveries suggest the excellent chromatographic column performance of the DEAE dextran-FF resins. Finally, both high recovery and purity of BSA and γ-globulin could be easily achieved using the typical DEAE dextran-FF column, FF-D60-DexD160, to separate their binary mixtures,by step gradient elution. The research has provided new insights into the practical application of the series of DEAE-dextran grafted resins in protein chromatography and proved their superiority. | Shu Bai Lingli Gong Detao Han Yutong Li Linling Yu Yan Sun | 2018 | Chinese Journal of Chemical Engineering2018，26，2： | 5 |
| 3 | Twisted intra-molecular electron transfer phenomenon of dansyl immobilized on chitosan film and its sensing property to the composition of ethanol-water mixtures显示文摘 | Ding Liping Fang Yu Jiang Linling | 2005 | Thin Solid Films2005，478，12： | 1 |
| 4 | Head-to-tail cyclization of a heptapeptide eliminates its cytotoxicity and significantly increases its inhibition effect on amyloid β-protein fibrillation and cytotoxicity显示文摘淀粉 --(A) 蛋白质聚集是 Alzheimers 疾病(广告) 的主要特点。纤维性颤动的抑制因此是广告的预防和处理的一条有希望的治疗学的途径。最近,我们设计了一个 heptapeptide 禁止者, LVFFARK (LK7 ) 。LK7 在纤维性颤动上显示出一个有希望的禁止的能力,但是对敏感自我装配并且显示高 cytotoxicity,它将妨碍它的实际申请。此处,我们由 head-to-tail 环合修改了 LK7 并且获得了周期的 LK7 (cLK7 ) 。cLK7 展览一不同自己组装从 LK7 的行为,并且比 LK7 和小 cytotoxicity 对解朊作用有更高的稳定性到 SHSY5Y 房间。热力学的分析表明 LK7 和 cLK7 能由静电的相互作用,氢结合和恐水病的相互作用绑在40,但是为40的 cLK7 的有约束力的亲密关系( K D = 4.96 mol/L )比 LK7 的高六倍( K D = 32.2 mol/L )。强壮的绑定使 cLK7 能稳定 40 的第二等的结构并且 potently 在广泛的集中范围禁止它的成核,纤维性颤动和 cytotoxicity,而 LK7 能仅仅中等在低集中禁止 A40 纤维性颤动和 cytotoxicity。调查结果显示肽环合是一条有希望的途径提高基于肽的淀粉的禁止者的表演。 | Shuai Ma Huan Zhang Xiaoyan Dong Linling Yu Jie Zheng Yan Sun | 2018 | Frontiers of Chemical Science and Engineering2018，12，2： | 1 |
| 5 | 查看详情显示文摘 | Yu Q Huang R F Li L F LinL Hang W He J Huang B L | | 0，，11： | 1 |
| 6 | Characterization of novel mixed-mode protein adsorbents fabricated from benzoyl-modified polyethyleneimine-grafted Sepharose显示文摘 | Na Liu Ziyuan Wang Xiaoguang (Margaret) Liu Linling Yu Yan Sun | 2014 | Journal of Chromatography A2014，，： | 1 |
| 7 | Implications of neuroimaging findings in addiction显示文摘Addiction is a chronic and often relapsing brain disorder characterized by drug abuse and withdrawal symptoms and compulsive drug seeking(Koob and Volkow,2010)when access to the drug is restricted.Addiction leads to structural and functional brain changes implicated in reward,memory,motivation,and control(Volkow et al.,2019;Lüscher et al.,2020). | Xinwen Wen Lirong Yue Zhe Du Linling Li Yuanqiang Zhu Dahua Yu Kai Yuan | 2023 | Psychoradiology2023，3，1： | 0 |
| 8 | Mining and utilization of salinity tolerant legumes in tropical coastal agroecosystems: An overview显示文摘Coastal saline soils are increasing year by year caused by climate change and human activities.Most of the coastal saline soils are idle due to their high salinity level and few crops can grow normally.Salinity tolerant legumes are naturally tolerant to salt,which can ecologically cover the coastal saline soil,enhance soil fertility by symbiotic nitrogen fixation and increase the smallholder farmers’benefits in terms of forage,green manure,food or medicine.However,few reports are available for the systematic evaluation of salinity tolerant legumes.This review summarizes and evaluates currently available salinity tolerant legume species that could potentially be used and discusses their potential for integration into smallholder mixed coastal systems of the Asia-Pacific region.Fourty four salinity tolerant legumes were summarized,six of them showed a high level of salinity tolerance,17 of them showed a moderate level of salinity tolerance and 21 of them showed potential salinity tolerance but need to be further studied.Many gaps such as combined tolerance evaluation,nitrogen fixation efficiency,animal feeding experiments and salinity tolerant rhizobia screening/inoculants exist.Case studies demonstrate legumes could be used to reclaim coastal saline soils,but commitment and support from government and public services are necessary to address both seed system and extension needs,through the provision of adequate incentives,policies and development efforts. | Yiming Liu Mary Atieno Juan Andres Cardoso Hubiao Yang Bin Xu Rongshu Dong Linling Yan Chunqiong Huang Hengfu Huan Daogeng Yu Sabine Douxchamps Guodao Liu | 2022 | Grass Research2022，2，1： | 0 |
| 9 | Study on Saccharification of Saccharum spontaneum L. by Sulphuric Acid Hydrolysis显示文摘In order to improve the saccharification rate by acid hydrolysis in the technique for production of ethanol from biomass,in this study,Saccharum spontaneum L. was used as the experimental material to investigate the rules and technological conditions for two-step acid-hydrolysis saccharification by single factor experiment and orthogonal experiment. According to the results,concentrated sulfuric acid hydrolysis was the first step,with the liquid-solid ratio of 42∶ 1,sulfuric acid concentration of 70%,hydrolysis time of 20 min,and hydrolysis temperature of 55 ℃; dilute sulphuric acid hydrolysis was the second step,with the liquidsolid ratio of 115∶ 1,sulfuric acid concentration of 5. 5%,hydrolysis time of 155 min,and hydrolysis temperature of 100 ℃. In two-step acid hydrolysis process,the yield of reducing sugar was 48. 78%. The results indicated that two-step acid-hydrolysis saccharification required mild conditions,simple operation and low cost,and led to high reducing sugar yield,exhibiting a broad application prospect. | Jianle LIU Changjun BAI Linling YAN Qinglin JIA Yu ZHANG Hubiao YANG | 2014 | Agricultural Biotechnology2014，3，6： | 0 |
| 10 | Nutritional Value Evaluation of Saccharum spontaneum L. Germplasm Resources显示文摘The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them, the crude protein, crude fat, neutral detergent fiber, acid detergent fiber, P and Ca contents were treated as the judging indicators. The nutritional value evaluation was carried out with the analytic hierarchy process(AHP). The results showed A49(Guangxi)had the highest nutritional value. Its dry matter, crude protein, crude fat, crude ash,neutral detergent fiber, acid detergent fiber, Ca and P contents were 32.35%,7.20%, 4.06%, 8.07%, 75.81%, 50.72%, 0.23% and 0.16% respectively. While A3(Guangdong) had the lowest nutritional value. Its dry matter, crude protein, crude fat, crude ash, neutral detergent fiber, acid detergent fiber, Ca and P contents were32.24%, 4.62%, 0.51%, 6.38%, 46.40%, 40.02%, 0.15% and 0.09% respectively.The crude protein content differed significantly among different germplasms. A48 had the highest crude protein content(9.11%), and A14 had the lowest crude protein content(3.72%). Based on the evaluation results, the 43 Saccharum spontaneum L.germplasms were divided into 3 groups: high-nutritional value type, moderate-nutritional value type and low-nutritional value type. We hoped to provide a theoretical reference for the application of Saccharum spontaneum L. as a forage grass. | Jianle LIU Changjun BAI Linling YAN Shimeng CHEN Yu ZHANG Hubiao YANG | 2014 | Agricultural Science & Technology2014，15，11： | 0 |
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