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| 1 | RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings显示文摘在有前列腺癌症的不同赛跑的病人之中有显著不同;然而,位于这差别下面的机制仍然保持不清楚。这里,我们在场 transcriptome 的一处全面风景用 RNA-seq,越过关于基因熔化的前列腺癌症 transcriptomes 的揭示巨大的差异,长 noncoding RNA (长 ncRNA ) ,其他的拼接和体的变化从中国人口 14 主要前列腺癌症和他们的配对的正常对应物介绍。14 个肿瘤(21.4%) 中的三个在中国病人怀有 TMPRSS2 尔格熔化,和这熔化的低流行进一步在一个另外的肿瘤集合(10/54=18.5%) 被证实。尤其是,二新奇基因熔化, CTAGE5-KHDRBS3 (20/54=37%) 和 USP9Y-TTTY15 (19/54=35.2%) ,在我们的耐心的队经常发生了。介绍的进一步系统的 transcriptional 识别了是在肿瘤表示的差别的众多的长 ncRNAs。在长 ncRNA 和基因的表示之间的关联的分析建议长 ncRNAs 可以在 transcriptional 规定以外有功能。这研究在中国人口产出新卓见进前列腺癌症的致病。 | Shancheng Ren Zhiyu Peng Jian-Hua Mao Yongwei Yu Changjun Yin Xin Gao Zilian Cui Jibin Zhang Kang Yi Weidong Xu Chao Chen Fubo Wang Xinwu Guo Ji Lu Jun Yang Min Wei Zhijian Tian Yinghui Guan Liang Tang Chuanliang Xu Linhui Wang Xu Gao Wei Tian Jian Wang Huanming Yang Jun Wang Yinghao Sun | 2012 | Cell Research2012，22，5： | 66 |
| 2 | Discovery of Metastasis-Associated Biomarkers in Ovarian Cancer Using SELDI-TOF: An in Vitro and Clinical Study显示文摘OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis. | Zhiguo Zheng Yun Gao Linhui Gu Hanzhou Mou Chihong Zhu Jianqing Zhu Shenhua Xu | 2009 | Clinical oncology and cancer resexreh2009，6，5： | 6 |
| 3 | Natural orifice transluminal endoscopic surgery in urology: The Chinese experience显示文摘Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice. | Xiaofeng Zou Guoxi Zhang Tianpeng Xie Yuanhu Yuan Rihai Xiao Gengqing Wu Xiaoning Wang Hui Xu Folin Liu Yuting Wu Yunfeng Liao Quanliang Liu Yinghao Sun Bo Yang Linhui Wang Chuanliang Xu Xiaofeng Gao | 2020 | Asian Journal of Urology2020，7，1： | 5 |
| 4 | Surface-grafted block copolymer brushes with continuous composition gradients of poly(poly(ethylene glycol)-monomethacrylate) and poly(N-isopropylacrylamide)显示文摘Surface-grafted block copolymer brushes with continuous composition gradients containing poly(poly(ethylene glycol) monomethacrylate) (P(PEGMA)) and poly(N-isopropylacrylamide) (PNIPAAm) chains were fabricated by integration of the surface-initiated atom transfer radical polymerization (SI-ATRP) and continuous injection method.Three types of copolymer gradients were prepared: (1) a uniform P(PEGMA) layer was block copolymerized with a gradient PNIPAAm layer (PP1);(2) a gradient P(PEGMA) layer was block copolymerized with a uniform PNIPAAm layer (PP2);and (3) a gradient P(PEGMA) layer was inversely block copolymerized with a gradient PNIPAAm layer (PP3).The as-prepared gradients were characterized by ellipsometry,water contact angle and atomic force microscopy (AFM) to determine their alterations in thickness,surface wettability and morphology,confirming the gradient structures.In vitro culture of HepG2 cells was implemented on the gradient surfaces,revealing that the cells could adhere at 37 °C and be detached at 20 °C.Introduction of the PEG chains as an underlying layer on the PNIPAAm grafting surfaces resulted in faster cell detachment compared with the PNIPAAm grafting surface. | LI LinHui WU JinDan GAO ChangYou | 2011 | Science China Chemistry2011，54，2： | 3 |
| 5 | Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction after failed endoscopic intervention:A technical report显示文摘Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine Valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction. | Zhixiang Wang Bing Liu Xiaofeng Gao Yi Bao Yang Wang Huamao Ye Yinghao Sun Linhui Wang | 2015 | Asian Journal of Urology2015，2，4： | 2 |
| 6 | Dye-sensitized solar cells based on anatase TiO 2 /multi-walled carbon nanotubes composite nanofibers photoanode显示文摘 | Pingfan Du Lixin Song Jie Xiong Ni Li Lijun Wang Zhenqiang Xi Naiyan Wang Linhui Gao Hongliang Zhu | 2013 | Electrochimica Acta2013，，： | 1 |
| 7 | Maternal genetic history of ancient Tibetans over the past 4000 years显示文摘The settlement of the Tibetan Plateau epitomizes human adaptation to a high-altitude environment that poses great challenges to human activity.Here,we reconstruct a 4000-year maternal genetic history of Tibetans using 128 ancient mitochondrial genome data from 37 sites in Tibet.The phylogeny of haplotypes M9a1a,M9a1b,D4g2,G2a’c,and D4i show that ancient Tibetans share the most recent common ancestor with ancient Middle and Upper Yellow River populations around the Early and Middle Holocene.In addition,the connections between Tibetans and Northeastern Asians vary over the past 4000 years,with a stronger matrilineal connection between the two during 4000 BPe3000 BP,and a weakened connection after 3000 BP,that are coincident with climate change,followed by a reinforced connection after the Tubo period(1400 BPe1100 BP).Besides,an over 4000-year matrilineal continuity is observed in some of the maternal lineages.We also find the maternal genetic structure of ancient Tibetans is correlated to the geography and interactions between ancient Tibetans and ancient Nepal and Pakistan populations.Overall,the maternal genetic history of Tibetans can be characterized as a long-term matrilineal continuity with frequent internal and external population interactions that are dynamically shaped by geography,climate changes,as well as historical events. | Ganyu Zhang Can Cui Shargan Wangdue Hongliang Lu Honghai Chen Lin Xi Wei He Haibing Yuan Tinley Tsring Zujun Chen Feng Yang Tashi Tsering Shuai Li Norbu Tashi Tsho Yang Yan Tong Xiaohong Wu Linhui Li Yuanhong He Peng Cao Qingyan Dai Feng Liu Xiaotian Feng Tianyi Wang Ruowei Yang Wanjing Ping Ming Zhang Xing Gao Yichen Liu Wenjun Wang Qiaomei Fu | 2023 | Journal of Genetics and Genomics2023，50，10： | 1 |
| 8 | Hydrothermal synthesis of blue-emitting YPO4:Yb3+ nanophosphor显示文摘 | Guangfa WANG Linhui GAO Hongliang ZHU Weijie ZHOU | 2016 | Frontiers of Materials Science2016，10，2： | 1 |
| 9 | Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol显示文摘The methylotrophic yeast Pichia pastoris(syn.Komagataella phaffii)has been extensively engineered for protein production,and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.However,the relatively unclear methanol metabolism hampers the metabolic rewiring to improve the biosynthetic efficiency.We here performed a label-free quantitative proteomic analysis of Pichia pastoris when cultivated in minimal media containing methanol and glucose,respectively.There were 243,158 up-regulated proteins and 244,304 down-regulated proteins in log and stationary phase,respectively,when cultivated in methanol medium compared with that of glucose medium.Peroxisome enrichment further improved the characterization of more differentially expressed proteins(481 proteins in log phase and 524 proteins in stationary phase).We demonstrated the transaldolase isoenzyme(Tal2,Protein ID:C4R244)was highly up-regulated in methanol medium cultivation,which plays an important role in methanol utilization.Our work provides important information for understanding methanol metabolism in methyltrophic yeast and will help to engineer methanol biotransformation in P.pastoris. | Rui Hou Linhui Gao Jianhui Liu Zhen Liang Yongjin J.Zhou Lihua Zhang Yukui Zhang | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 1 |
| 10 | Variation of peroxidase isoenzyme and biofilm of Phanerochaete chrysosporium in continuous membrane bioreactor for Reactive Brilliant Red X3-B treatment显示文摘The inuence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme of Phanerochaete chrysosporium was determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor (MBR) was also investigated by scanning electron microscope (SEM). The variation of peroxidase isoenzyme and the decolorization rate in the continuous MBR were evaluated. The results showed that the 100 mg/L RBR X-3B could stimulate the production of the peroxidase isoenzyme in the shaking-ask culture. In addition, two new peroxidase isoenzyme bands with relative mobility (Rf) value of 0.27 and 0.28 appeared, but the activity was lower than the blank control of 11 d. In the continuous MBR, the system worked stably during the first 60 d, the main peroxidase isoenzyme bands existed and three new bands with Rf value of 0.10, 0.27, and 0.28 appeared. Meanwhile, the biofilm grew well and the average decolorization rate could reach 90.6%. But the bands of peroxidase isoenzyme decreased rapidly at day 65, only two bands with Rf value 0.24 and 0.26 existed, and the decolorization rate decreased to 78.3%. Therefore, 5 bottles of P. chrysosporium mycelial pellet were added into the MBR, and then the activity of the peroxidase isoenzyme and the decolorization rate had a slight recovery. Finally, the decolorization rate finally decreased to 75.2%. These results contribute to a comprehensive understanding of the variation of peroxidase isoenzyme and biofilm in continuous MBR by white rot fungi. | GAO Shang, CHEN Cheng, TAO Fang, HUANG Minsheng, MA Lihua, WANG Zhonghua, WU Linhui School of Resource and Environment Science, East China Normal University, Shanghai 200062, China. | 2009 | Journal of Environmental Sciences2009，21，7： | 1 |
| 11 | Silencing of CXCR4 by RNA interference inhibits cell growth and metastasisin human renal cancer cells显示文摘 | Linhui Wang Ting Huang Wei Chen Xiaofeng Gao Tie Zhou Zhenjie Wu Yinghao Sun | 2012 | Oncology Reports2012，，6： | 1 |
| 12 | Electrochemical properties of amorphous Mg-Fe alloys mixed with Ni prepared by ball-milling 显示文摘 | Xiao Xuezhang Wang Xinhua Gao Linhui | 2006 | Journal of Alloys and Compounds2006，413，： | 1 |
| 13 | Analysis of HK-2 cells exposed to oxalate and calcium oxalate crystals: proteomic insights into the molecular mechanisms of renal injury and stone formation显示文摘 | Shushang Chen Xiaofeng Gao Yinghao Sun Chuanliang Xu Linhui Wang Tie Zhou | 2010 | Urological Research2010，，1： | 1 |
| 14 | Covalently immobilized gelatin gradients within three-dimensional porous scaffolds显示文摘A stable gelatin gradient providing continuous increment of signaling for cell adhesion and proliferation was fabricated within 3D poly(L-lactic acid) (PLLA) scaffolds. The porous PLLA scaffold fabricated by NaCl particle leaching was vertically fixed on a glass vial. 1,6-Hexanediamine/propanol solution was continuously injected into the vial by a micropump to aminolyze the PLLA scaffold. As a result of reaction time difference, the introduced -NH2 groups increased continuously along with the longitude of the PLLA scaffold in the z-direction. After covalent immobilization of gelatin by glutaraldehyde coupling, the gelatin gradient scaffold was thus obtained. In vitro chondrocyte culture showed that the cells had higher viability and more extending morphology in the gelatin gradient scaffold than that in the uniform gelatin control. | WU JinDan TAN HuaPing LI LinHui GAO ChangYou | 2009 | Chinese Science Bulletin2009，54，18： | 1 |
| 15 | Silencing of CXCR4 by RNA interference inhibits cell growth and metastasisin human renal cancer cells显示文摘 | Linhui Wang Ting Huang Wei Chen Xiaofeng Gao Tie Zhou Zhenjie Wu Yinghao Sun | 2012 | Oncology Reports2012，，6： | 1 |
| 16 | Electrochemical properties of the ball-milled LaMg10 NiMn alloy with Ni powders 显示文摘 | Wang Yi Wang Xinhua Gao Linhui | 2008 | Materials Chemistry and Physics2008，110，： | 1 |
| 17 | New dating indicates intermittent human occupation of the Nwya Devu Paleolithic site on the high-altitude central Tibetan Plateau during the past 45,000 years显示文摘The timing and mechanisms of the human occupation of the demanding high-altitude Tibetan Plateau environment are of great interest.Here,we report on our reinvestigations and dating of the Nwya Devu site,located nearly 4600 meters above sea level on the central Tibetan Plateau.A new microblade techno-complex was identified on a lower lake shore at this site,distinct from the previously reported blade tool assemblage.These two lithic assemblages were dated to 45.6±2.6 and10.3±0.5 ka using optically stimulated luminescence and accelerator mass spectrometry^(14)C methods.They represent,respectively,the earliest known Paleolithic and microlithic sites on the interior Tibetan Plateau,indicating multiple occupation episodes of hunter-gatherers during the past 45 ka.Our studies reveal that relatively stable depositional conditions and a paleoenvironment characterized by a comparatively warm climate facilitated these multiple occupations at Nwya Devu.The contemporaneous occurrence of the Upper Paleolithic blade technology on the Tibetan Plateau and most of Eurasia between 50 and 40 ka indicates rapid,large-scale dispersals of humans that profoundly affected human demography on a large scale.Combining new archaeological evidence and previously reported genetic data,we conclude that the Tibetan Plateau provided a relatively stable habitat for Upper Paleolithic hunter-gatherers,which may have contributed to the complex and multiple-origin gene pool of present-day Tibetans. | Junyi GE Xiaoling ZHANG Shejiang WANG Linhui LI Wei HE Yingshuai JIN Peiqi ZhANG Bing XU Chenglong DENG John WOLSEN Zhengtang GUO Xing GAO | 2024 | Science China Earth Sciences2024，67，2： | 0 |
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