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| 1 | Determination of rice panicle numbers during heading by multi-angle imaging显示文摘Plant phenomics has the potential to accelerate progress in understanding gene functions and environmental responses. Progress has been made in automating high-throughput plant phenotyping. However, few studies have investigated automated rice panicle counting. This paper describes a novel method for automatically and nonintrusively determining rice panicle numbers during the full heading stage by analyzing color images of rice plants taken from multiple angles. Pot-grown rice plants were transferred via an industrial conveyer to an imaging chamber. Color images from different angles were automatically acquired as a turntable rotated the plant. The images were then analyzed and the panicle number of each plant was determined. The image analysis pipeline consisted of extracting the i2 plane from the original color image, segmenting the image, discriminating the panicles from the rest of the plant using an artificial neural network, and calculating the panicle number in the current image. The panicle number of the plant was taken as the maximum of the panicle numbers extracted from all 12 multi-angle images. A total of 105 rice plants during the full heading stage were examined to test the performance of the method. The mean absolute error of the manual and automatic count was 0.5, with 95.3% of the plants yielding absolute errors within ± 1. The method will be useful for evaluating rice panicles and will serve as an important supplementary method for high-throughput rice phenotyping. | Lingfeng Duan Chenglong Huang Guoxing Chen Lizhong Xiong Qian Liu Wanneng Yang | 2015 | The Crop Journal2015，3，3： | 18 |
| 2 | Crystal structure of cytotoxin protein suilysin from Streptococcus suis显示文摘Cholesterol-dependent cytolysins(CDC)are pore forming toxins.A prototype of the CDC family members is perfringolysin O(PFO),which directly binds to the cell membrane enriched in cholesterol,causing cell lysis.However,an exception of this general observation is intermedilysin(ILY)of Streptococcus intermedius,which requires human CD59 as a receptor in addition to cholesterol for its hemolytic activity.A possible explanation of this functional difference is the conformational variation between the C-terminal domains of the two toxins,particularly in the highly conserved undecapeptide termed tryptophan rich motif.Here,we present the crystal structure of suilysin,a CDC toxin from the infectious swine pathogen Streptococcus suis.Like PFO,suilysin does not require a host receptor for hemolytic activity;yet the crystal structure of suilysin exhibits a similar conformation in the tryptophan rich motif to ILY.This observation suggests that the current view of the structure-function relationship between CDC proteins and membrane association is far from complete. | Lingfeng Xu Bo Huang Huamao Du Xuejun CZhang Jianguo Xu Xuemei Li Zihe Rao | 2010 | Protein & Cell2010，1，1： | 9 |
| 3 | Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum显示文摘Exploiting the negative biochemical interference between plants and algal species has been suggested as a method to control harmful algal blooms.In this work,we investigated the inhibitory effect of the salt marsh halophyte Salicornia europaea against the marine alga Skeletonema costatum.S.europaea suppressed the growth of S.costatum in a nutrient-sufficient co-culture system,indicating that the inhibition of algal growth was because of the phytotoxic effect of S.europaea,rather than nutrient competition.We tested aqueous and organic extracts from S.europaea roots against S.costatum.The organic extracts inhibited growth and affected the cell size and chlorophyll a content of S.costatum in a dose-dependent manner.Among the three tested organic extracts,the methanol extract had the greatest effects on S.costatum,followed by butanol extract,and then the chloroform extract.Two flavonoids,rutin and quercetin-3-D-glucoside,were identified in the methanol extract by high performance liquid chromatography.The concentration of rutin was much higher than that of quercetin-3-D-glucoside.In an algal bioassay,rutin inhibited the growth of S.costatum and the inhibitory effect increased with increasing rutin concentration and with decreasing initial algal density.Therefore,we concluded that S.europaea negatively affects the growth of S.costatum,and that rutin,a metabolite of S.europaea,may play a role in this inhibitory effect. | JIANG Dan HUANG LingFeng LIN YongQing NIE LingLing LV SuLian KUANG TingYun LI YinXin | 2012 | Science China(Life Sciences)2012，55，6： | 8 |
| 4 | Factors regulating population dynamics of the amphipod Ampithoe valida in a eutrophic subtropical coastal lagoon显示文摘Year-round investigations were carried out to address the population dynamics of amphipod Ampithoe valida and its potential regulating factors in the Yundang Lagoon (a eutrophic subtropical coastal lagoon in Xiamen City, China) from October 2007 to October 2008. The results show that A. valida population was largely composed of juveniles and reached a peak abundance (12.02 × 10 3 ind./m2 ) in March, but sharply shrunk in July. The monthly variation pattern of A. valida seemed to follow that of Ulva lactuca which is the main food resource for A. valida, indicating a strong influence of food availability on A. valida population dynamics. Life cycle studies in laboratory indicate that A. valida adopted an r-selected life strategy, e.g., rapid growth rate (0.216-0.302 mm/d), consecutive breeding (4-10 times), short reproductive rhythm (9-17 d) and huge fecundity (24-192 eggs or 6-94 juveniles per brood), which contributed to the fast population growth of A. valida from January to March. Although temperature may also be a significant cause, A. valida could grow quickly and show normal reproductive traits (i.e., the timing of sexual differentiation or maturity, the reproductive rhythm, the number of broods all through its lifespan and offspring production) in temperature between 15-30 C. Therefore, the temperature variation in the lagoon water should not account for the sharp variation of A. valida population by affecting the survival, growth and reproduction of the amphipod. However, negative correlation between water temperature and U. lactuca biomass in the lagoon suggested that temperature could have indirectly affected the population dynamics of A. valida by affecting its food availability. The authors conclude that, in the Yundang Lagoon, the population dynamics of A. valida was mainly controlled by the food availability rather than water temperature. | ZHENG Xinqing HUANG Lingfeng HUANG Bangqin LIN Yongqing | 2013 | Acta Oceanologica Sinica2013，32，6： | 5 |
| 5 | Flexible, transparent nanocellulose paper-based perovskite solar cells显示文摘Recently perovskite solar cells(PSCs),as photoelectric conversion devices,exhibit excellent power conversion efficiency(PCE)and low-processing cost,and have become one of the most promising devices to replace conventional silicon-based solar cells and address current pressing energy issues.Among them,the flexible PSCs are especially more widely applicable and may propel the rapid advancements of wearable electronics,causing a significant paradigm shift in consumer electronics.Current flexible PSCs use non-biodegradable petroleum-based polymer substrates,discarding of which will aggravate“white pollution”.Therefore,development of green,biodegradable and low-cost flexible substrates will provide a great alternative to flexible PSCs.Here we have developed transparent nanocellulose paper(NCP)with coating of acrylic resin as substrates to fabricate flexible PSCs,which are biodegradable and easily disposable.The PCE of these NCP-based PSCs reached 4.25%,while the power per weight(the ratio of power to device weight)was as high as 0.56 W g^(–1).The flexible PSCs also showed good stability,retaining>80%of original efficiency after 50 times of bending.The NCP-based substrates can also be applied to other electronic systems,which may prosper next-generation green flexible electronics. | Lei Gao Lingfeng Chao Meihui Hou Jin Liang Yonghua Chen Hai-Dong Yu Wei Huang | 2019 | npj Flexible Electronics2019，3，1： | 5 |
| 6 | Quantitative relationship between flagellate abundance and suspended particle density in Huanghai Sea and East China Sea in summer显示文摘调查在 Huanghai 海和华东 Seato 学习被执行在许多之间的量的关系鞭打并且在 2001 的夏天的推迟的粒子的密度。许多鞭打的结果表演从 44 ~ 1 改变 2600 cell/cm~3,并且有时鞭打组成推迟的粒子的重要部分。推迟的粒子的尺寸光谱能被划分成四个范畴:扁平的光谱,弓着的光谱,浮游生物光谱和混合光谱。一般来说,许多鞭打与推迟的粒子的密度成比例变化。然而,他们的量的关系在不同类型 ofparticle 尺寸光谱的海水样品揭示不同特征。这仅仅是在之间的量的关系的初步的研究鞭打并且推迟了粒子,它可能导致一条潜在的方便途径到评价鞭打在海的丰富。 | HUANG Lingfeng PAN Ke GUO Feng | 2006 | Acta Oceanologica Sinica2006，25，2： | 3 |
| 7 | Recent progress on low dimensional perovskite solar cells显示文摘Low dimensional perovskites have recently attracted much attention due to their vertical growth of crystalline orientation, excellent film morphology, and long-term humidity, light, and heat stability. However, low dimensional perovskites suffer from low power conversion efficiency(PCE) with respect to their three dimensional analogues. Therefore, it is imperative to find excellent low-dimensional perovskite materials for improving the PCE. Previous work has demonstrated that bulkier organic molecules,e.g., C_6 H_5(CH_2)_2 NH_3^+(PEA^+), CH_3(CH_2)_3 NH_3^+(n-BA+, iso-BA+), C_2H_4NH_3^+, and polyethylenimine cations(PEI+), play an important role in the formation of low-dimensional perovskites. In this review, we review the recent development of low dimensional perovskites for solar cells application in terms of film preparation, photophysics, and stability of perovskites, as well as the related device structure and physics. We have also discussed the future development of low-dimensional perovskites from materials design, fabrication processes, and device structure. | Lingfeng Chao Ze Wang Yingdong Xia Yonghua Chen Wei Huang | 2018 | Journal of Energy Chemistry2018，27，4： | 3 |
| 8 | High-Performance Photo-Modulated Thin-Film Transistor Based on Quantum dots/Reduced Graphene Oxide Fragment-Decorated ZnO Nanowires显示文摘In this paper, a photo-modulated transistor based on the thin-film transistor structure was fabricated on the flexible substrate by spin-coating and magnetron sputtering. A novel hybrid material that composed of Cd Se quantum dots and reduced graphene oxide(RGO) fragment-decorated ZnO nanowires was synthesized to overcome the narrow optical sensitive waveband and enhance the photo-responsivity. Due to the enrichment of the interface and heterostructure by RGO fragments being utilized, the photo-responsivity of the transistor was improved to 2000 AW^(-1) and the photo-sensitive wavelength was extended from ultraviolet to visible. In addition, a positive back-gate voltage was employed to reduce the Schottky barrier width of RGO fragments and ZnO nanowires. As a result, the amount of carriers was increased by 10 folds via the modulation of back-gate voltage. With these inherent properties, such as integrated circuit capability and wide optical sensitive waveband, the transistor will manifest great potential in the future applications in photodetectors. | Zhi Tao Yi-an Huang Xiang Liu Jing Chen Wei Lei Xiaofeng Wang Lingfeng Pan Jiangyong Pan Qianqian Huang Zichen Zhang | 2016 | Nano-Micro Letters2016，8，3： | 2 |
| 9 | Rationally exfoliating chitin into 2D hierarchical porous carbon nanosheets for high-rate energy storage显示文摘Two-dimensional(2D)carbon nanomaterials with hierarchical porous structure and heteroatoms doping are highly desirable in the fields of energy storage because of their rich active surface and open ion diffusion channels.However,the scalable preparation of carbon materials simultaneously possessing ultrathin 2D feature and hierarchical pores remains a considerable challenge.Herein,a facile one-step method to massively fabricate 2D porous chitin nanosheets(coded as PCNs)via a phytic acid assisted top-down exfoliation of bulk chitin under hydrothermal treatment was presented.Subsequently,2D carbon nanosheets with extra-thin thickness(3.6 nm),well-defined hierarchical porosity,high specific surface area(855 m2·g^-1),as well as abundant self-doped heteroatoms(N,O,P)were fabricated by carbonizing the PCNs,and was named as HPCNs.The as-obtained HPCNs demonstrated remarkable electrochemical performance as electrode material for supercapacitors.The symmetric supercapacitors(SSCs)based on HPCNs exhibited a high specific capacitance of 79 F·g^-1(316 F·g^-1 for single electrode)in 6 M KOH aqueous electrolyte solution,as well as a remarkable energy density of 23.8 W·h·kg^-1 by using 1 M Li2SO4 as electrolyte.It is also demonstrated that HPCNs/PCNs hybrid dispersions can be used as inks to fabricate conductive films and energy devices with high strength and superior flexibility.This work paves a new avenue for the economical and large-scale synthesis of 2D hierarchically porous carbon materials for energy storage related applications. | Lingfeng Gao Guoqun Zhang Jie Cai Liang Huang Jun Zhou Lina Zhang | 2020 | Nano Research2020，13，6： | 2 |
| 10 | Origin of High Efficiency and Long-Term Stability in Ionic Liquid Perovskite Photovoltaic显示文摘Environment-friendly protic amine carboxylic acid ionic liquids(ILs)as solvents is a significant breakthrough with respect to traditional highly coordinating and toxic solvents in achieving efficient and stable perovskite solar cells(PSCs)with a simple one-step air processing and without an antisolvent treatment approach.However,it remains mysterious for the improved efficiency and stability of PSCs without any passivation strategy.Here,we unambiguously demonstrate that the three functions of solvents,additive,and passivation are present for protic amine carboxylic acid ILs.We found that the ILs have the capability to dissolve a series of perovskite precursors,induce oriented crystallization,and chemically passivate the grain boundaries.This is attributed to the unique molecular structure of ILs with carbonyl and amine groups,allowing for strong interaction with perovskite precursors by forming C=O…Pb chelate bonds and N-H…I hydrogen bonds in both solution and film.This finding is generic in nature with extension to a wide range of IL-based perovskite optoelectronics. | Lingfeng Chao Tingting Niu Hao Gu Yingguo Yang Qi Wei Yingdong Xia Wei Hui Shouwei Zuo Zhaohua Zhu Chengjie Pei Xiaodong Li Jing Zhang Junfeng Fang Guichuan Xing Hai Li Xiao Huang Xingyu Gao Chenxin Ran Lin Song Li Fu Yonghua Chen Wei Huang | 2020 | Research2020，，1： | 2 |
| 11 | Cy5.5-MSA-G250 nanoparticles(CMGNPs)induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner显示文摘Cy5.5-MSA-G250 nanoparticles(CMGNPs)had been proved to have unique advantages for cancer treatment,including excellent photothermal performance,tumor cell-selective cytotoxicity,direct visualization,and good biocompatibility.However,to cellular systems,the CMGNPs are considered as fo reign invaders,and the effect of CMGNPs on immunity system is still unknown.Therefore,more efforts are needed to understand the role of CMGNPs on the immunity system.In this study,we attempted to screen the pro-inflammatory responses on RAW264.7 macrophages after treated with the CMGNPs.In vitro experiments clearly showed that CMGNPs not only enhances phagocytosis capacity of RAW264.7 cells,but also promotes Ml polarization,associated with changes in cell morphology and increased expression of inflammatory cytokines.This ability to induce Ml polarization may be beneficial to CMGNPs to achieve better anticancer effects in clinical trials.Moreover,the observed Ml macrophages’ polarization triggered by CMGNPs can be abolished after adding TLR4 inhibitor,CLI095,suggesting that TLR4 is involved in CMGNP-induced inflammation. | Zhuoxuan Lu Lingfeng Xu Nongyue He Fengying Huang Tiefeng Xu Li Li Yanwei Zhang Liming Zhang | 2019 | Chinese Chemical Letters2019，30，6： | 2 |
| 12 | Grazing by microzooplankton and copepods on the microbial food web in spring in the southern Yellow Sea,China显示文摘Assessment of microzooplankton and copepods grazing pressure on picoplankton is a key requirement for resolving the microbial food web efficiency.Although microzooplankton grazing on picoplankton has been extensively studied,the impact of microzooplankton on different groups of picoplankton,i.e.,heterotrophic bacteria,Synechococcus and picoeukaryotes have rarely been compared.Furthermore,in the very few existing studies there is no consistent evidence of an enhancing or restraining effect of copepods on picoplankton.More studies are needed to improve our understanding of the influence of microzooplankton and copepod on picoplankton.Dilution incubations and copepod addition incubations were performed during a cruise to the southern Yellow Sea on May 16-29,2007.The bulk grazing of microzooplankton and the calanoid copepod Cal anus sinicus on phytoplankton,flagellates and picoplankton was estimated.Stations were divided into either eutrophic or oligotrophic according to the nutrient and biological parameters.Picoplankton comprised a large part of the diet of microzooplankton in the central oligotrophic area,while phytoplankton was the main food of microzooplankton in the coastal eutrophic area.In the central oligotrophic area,microzooplankton preferred grazing on Synechococcus.After copepod addition,ciliate abundance decreased while Synechococcus abundance increased(382%,64%and 64%at three experimental stations,respectively),indicating strong grazing pressure of microzooplankton on Synechococcus.Our results suggest that Synechococcus might be an essential carbon source the planktonic food web in the oligotrophic waters of southern Yellow Sea. | Yuan Zhao Yi Dong Haibo Li Shiquan Lin Lingfeng Huang Tian Xiao Gerald Gregori Li Zhao Wuchang Zhang | 2020 | Marine Life Science & Technology2020，2，4： | 1 |
| 13 | Amphipods fail to suppress the accumulation of Ulva lactuca biomass in eutrophic Yundang Lagoon显示文摘The Gross growth efficiency(GGE) is defined as the amount of consumer carbon produced relative to the amount of prey ingested in a given time interval. In population ecology, it can be considered as an index for the ability of a consumer to convert the biomass of food into its population production. In this study, it was firstly applied to estimate the role of amphipods grazing in the biomass dynamics of macroalgae(Ulva lactuca) in Yundang Lagoon(a subtropical eutrophic coastal lagoon, Xiamen City, China). It was found that amphipods were the dominant group(82.2%–98.7%) in the benthic assemblage, in which five amphipod species(Corophium uenoi, Ampithoe valida, Grandidierella japonica, Grammaropsis laevipalmata and Caprella panantis) were observed in the lagoon. However, only A. valida significantly consumed U. lactuca among these amphipod species. A series of experiments were conducted to estimate the consumption of U. lactuca by A. valida. It was suggested that the monthly secondary production(P) of A. valida using size frequency method ranged from 2.39 to 13.68 g/m2. GGE of A. valida exponentially reduced with body size and was closely associated with water temperature. Therefore, GGE equation for different months using the temperature recorded during the field investigation was fitted. The monthly consumption of U. lactuca was figured out to be 86.7–1 549.0 g/m2 by the sum of consumption(P/GGE) by A. valida at different size, accounting for 2.0%–37.5% of U. lactuca production. It indicates that the grazing effect of A. valida did play a role in reducing the accumulation of U. lactuca biomass when A. valida was abundant, but it finally fails to limit the U. lactuca growth in Yundang Lagoon. Low abundance of A. valida and high nutrient loading may be two major reasons for the outbreak of U. lactuca bloom in the lagoon. | ZHENG Xinqing HUANG Lingfeng WANG Qian LIN Rongcheng | 2014 | Acta Oceanologica Sinica2014，33，12： | 1 |
| 14 | Preparation of nitrogen-doped titanium dioxide with visiblelight photocatalytic activity using a facile hydrothermal method显示文摘 | PENG Feng CAI Lingfeng HUANG Lei | 2008 | Journal of Physics and Chemistry of Solids2008，69，7： | 1 |
| 15 | Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ^(15)N) analysis of otoliths显示文摘Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT and stomach content data are insufficient for trophic level(TL)data of past fi shes which is important for the changes of marine fi shery resources over long time scales.To determine the correlation betweenδ^(15) N_(oto) and fi sh WMTδ^(15) N(δ^(15) N_(wmt))and the feasibility of usingδ^(15) N_(oto) in characterizing the TLs of marine fi shes,we conducted nitrogen stable isotope analysis(SIA)in the otolith and WMT of 36 marine fi sh species sampled from the Yellow Sea and northern East China Sea in 2011-2014.Bothδ^(15) N_(oto) andδ^(15) N_(wmt) were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer(EA-IRMS).Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step.δ^(15) N_(oto) andδ^(15) N_(wmt) comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for whichδ^(15) N_(oto) andδ^(15) N_(wmt) have been compared.Additionally,to determine the relative accuracy of trophic level calculated usingδ^(15) N_(oto),we compared TL calculated fromδ^(15) N_(oto) to traditional trophic level metrics calculated usingδ^(15) N_(wmt).The results showed a positive and highly signifi cant correlation(R=0.780,P<0.001)betweenδ^(15) N_(oto) andδ^(15) N_(wmt).Trophic level estimation using WMT(TL wmt)and otolith(TL oto)showed congruence in our study,which is not entirely surprising given thatδ^(15) N_(oto) was regressed againstδ^(15) N_(wmt) and the resulting regression coefficient was used to convertδ^(15) N_(oto) toδ^(15) N_(wmt) prior to calculating TL oto.This conversion was required in order to be consistent with previousδ^(15) N_(wmt)-based calculations of TL for comparison.TL oto calculations resulted in TL values that were largely within 5%-10%of TL values calculated withδ^(15) N_(wmt).Our fi ndings show thatδ^(15) N_(oto) is a feasible technique for characterizing the TLs of marine fi sh and can also assist in food web and marine ecosystem studies. | Huaiyu BAI Yukun WANG Tingting ZHANG Fangqun DAI Lingfeng HUANG Yao SUN | 2022 | Journal of Oceanology and Limnology2022，40，2： | 1 |
| 16 | Rice panicle length measuring system based on dual-camera imaging显示文摘 | Huang Chenglong Yang Wanneng Duan Lingfeng | 2013 | Computers and Electronics in Agriculture2013，98，： | 1 |
| 17 | Interferon-β-armed oncolytic adenovirus induces both apoptosis and necroptosis in cancer cells显示文摘干扰素 --(IFN-) 广泛地在癌症治疗被使用了,但是临床的试用结果通常是令人失望的。我们的以前的研究证明了带 IFN-(ZD55-IFN-) 的 oncolytic 侵入人体气管粘膜的病菌展出重要反肿瘤活动。然而,内在的机制不是清楚的。这里,我们由激活混乱毛细管扩张以一种 p53 依赖的方式显示出那 ZD55-IFN- 导致感染的 S 阶段房间周期拘捕变异依赖者的 DNA 损坏小径。另外, ZD55-IFN- 感染能在癌症房间开始 caspase 依赖的 apoptosis 和 necroptosis。更重要地, ZD55-IFN- 在癌症房间上看了 synergistic 效果什么时候与 doxorubicin 结合了。这些结果建议有 doxorubicin 的 ZD55-IFN- 的联合可以在癌症治疗代表有希望的临床的策略。 | Hongling Huang Tian Xiao Lingfeng He Hongbin Ji Xin-Yuan Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，9： | 1 |
| 18 | Two‑Dimensional Black Phosphorus Nanomaterials:Emerging Advances in Electrochemical Energy Storage Science显示文摘Two-dimensional black phosphorus(2D BP),well known as phosphorene,has triggered tremendous attention since the first discovery in 2014.The unique puckered monolayer structure endows 2D BP intriguing properties,which facilitate its potential applications in various fields,such as catalyst,energy storage,sensor,etc.Owing to the large surface area,good electric conductivity,and high theoretical specific capacity,2D BP has been widely studied as electrode materials and significantly enhanced the performance of energy storage devices.With the rapid development of energy storage devices based on 2D BP,a timely review on this topic is in demand to further extend the application of 2D BP in energy storage.In this review,recent advances in experimental and theoretical development of 2D BP are presented along with its structures,properties,and synthetic methods.Particularly,their emerging applications in electrochemical energy storage,including Li−/K−/Mg−/Na-ion,Li–S batteries,and supercapacitors,are systematically summarized with milestones as well as the challenges.Benefited from the fast-growing dynamic investigation of 2D BP,some possible improvements and constructive perspectives are provided to guide the design of 2D BP-based energy storage devices with high performance. | Junye Cheng Lingfeng Gao Tian Li Shan Mei Cong Wang Bo Wen Weichun Huang Chao Li Guangping Zheng Hao Wang Han Zhang | 2020 | Nano-Micro Letters2020，12，12： | 1 |
| 19 | A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy显示文摘With an intensive understanding of the mechanism of immune system,developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer immunotherapy.In this study,we report a facile approach to prepare graphene oxide(GO)-based therapeutic cancer-nanovaccine.The model antigen(ovalbumin,OVA)and adjuvant(CpG ODN),are conjugated with GO-PEI nanosheet through electrostatic interaction.The addition of PEG can improve biocompatibility and prevent nanoparticle aggregation.The prepared GO-based nanovaccine,GO-PEI-OVA-PEG-CpG,exhibits good biocompatibility and low toxicity both in vivo and in vitro.More importantly,it can efficiently induce the maturation of dendritic cells(DCs),the enhancement of antigen cross-presentation ability,and the amplification of cytokine production of immune cells.Impressively,this nanovaccine shows a remarkable therapeutic effect against preestablished B16-OVA-melanoma tumors,which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing mice.Moreover,combining GO-PEI-OVA-PEG-CpG with NLG919,an IDO-1(indoleamine-2,3-dioxygenase)inhibitor which can regulate the tumor microenvironment,displays a synergistic therapeutic effect.These findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes. | Liming Zhang Lingfeng Xu Yi Wang Jieyu Liu Guanghong Tan Fengying Huang Nongyue He Zhuoxuan Lu | 2022 | Chinese Chemical Letters2022，33，8： | 1 |
| 20 | AIPI suppresses atherosclerosis by limiting hyperlipidemia-induced inflammation and vascular endothelial dysfunction 显示文摘 | HUANG Qunhua QIN Lingfeng DAI Shengchuan | 2013 | Arterioscl Throm Vasculr Bio ( ATVB )2013，33，4： | 1 |
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