|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Telomere-to-telomere and gap-free reference genome assembly of the kiwifruit Actinidia chinensis显示文摘Kiwifruit is an economically and nutritionally important fruit crop with extremely high contents of vitamin C.However,the previously released versions of kiwifruit genomes all have a mass of unanchored or missing regions.Here,we report a highly continuous and completely gap-free reference genome of Actinidia chinensis cv.‘Hongyang’,named Hongyang v4.0,which is the first to achieve two de novo haploid-resolved haplotypes,HY4P and HY4A.HY4P and HY4A have a total length of 606.1 and 599.6 Mb,respectively,with almost the entire telomeres and centromeres assembled in each haplotype.In comparison with Hongyang v3.0,the integrity and contiguity of Hongyang v4.0 is markedly improved by filling all unclosed gaps and correcting some misoriented regions,resulting in∼38.6–39.5 Mb extra sequences,which might affect 4263 and 4244 protein-coding genes in HY4P and HY4A,respectively.Furthermore,our gap-free genome assembly provides the first clue for inspecting the structure and function of centromeres.Globally,centromeric regions are characterized by higher-order repeats that mainly consist of a 153-bp conserved centromere-specific monomer(Ach-CEN153)with different copy numbers among chromosomes.Functional enrichment analysis of the genes located within centromeric regions demonstrates that chromosome centromeres may not only play physical roles for linking a pair of sister chromatids,but also have genetic features for participation in the regulation of cell division.The availability of the telomere-to-telomere and gap-free Hongyang v4.0 reference genome lays a solid foundation not only for illustrating genome structure and functional genomics studies but also for facilitating kiwifruit breeding and improvement. | Junyang Yue Qinyao Chen Yingzhen Wang Lei Zhang Chen Ye Xu Wang Shuo Cao Yunzhi Lin Wei Huang He Xian Hongyan Qin Yanli Wang Sijia Zhang Ying Wu Songhu Wang Yi Yue Yongsheng Liu | 2023 | Horticulture Research2023，10，2： | 4 |
| 2 | A new method to solve the damped nonlinear Klein-Gordon equation显示文摘This paper discusses a damped nonlinear Klein-Gordon equation in the reproducing kernel space and provides a new method for solving the damped nonlinear Klein-Gordon equation based on the reproducing kernel space.Two numerical examples are given for illustrating the feasibility and accuracy of the method. | LIN YingZhen CUI MingGen | 2008 | Science China Mathematics2008，51，2： | 3 |
| 3 | Repre- sentation of the exact solution for a kind of nonlinear partial differential equations显示文摘 | LIN Yingzhen CUI Minggen YANG Lihong | 2006 | Applied Mathematics Letters2006，19，： | 1 |
| 4 | L Representation of the exact so- lution for a kind of nonlinear differential equations显示文摘 | Lin Yingzhen Cui Minggen | 2006 | Appl Math Lett2006，19，： | 1 |
| 5 | Solving singular boundary-value problems of higher even-order显示文摘 | YAO Huanmin LIN Yingzhen | | 0，，： | 1 |
| 6 | Numerical algorithm for parabolic problems with non-classical conditions显示文摘 | ZHOU Yongfang CUI Minggen LIN Yingzhen | | 0，，： | 1 |
| 7 | Effect of aging on islet beta-cell function and its mechanisms in Wistar rats显示文摘 | Zhaoyan Gu Yingzhen Du Yu Liu Lichao Ma Lin Li Yanping Gong Hui Tian Chunlin Li | 2012 | AGE2012，，6： | 1 |
| 8 | quarTeT: a telomere-to-telomere toolkit for gap-free genome assembly and centromeric repeat identification显示文摘A high-quality genome is the basis for studies on functional,evolutionary,and comparative genomics.The majority of attention has been paid to the solution of complex chromosome structures and highly repetitive sequences,along with the emergence of a new‘telomere-to-telomere(T2T)assembly’era.However,the bioinformatic tools for the automatic construction and/or characterization of T2T genome are limited.Here,we developed a user-friendly web toolkit,quarTeT,which currently includes four modules:AssemblyMapper,GapFiller,TeloExplorer,and CentroMiner.First,AssemblyMapper is designed to assemble phased contigs into the chromosome-level genome by referring to a closely related genome.Then,GapFiller would endeavor to fill all unclosed gaps in a given genome with the aid of additional ultra-long sequences.Finally,TeloExplorer and CentroMiner are applied to identify candidate telomere and centromere as well as their localizations on each chromosome.These four modules can be used alone or in combination with each other for T2T genome assembly and characterization.As a case study,by adopting the entire modular functions of quarTeT,we have achieved the Actinidia chinensis genome assembly that is of a quality comparable to the reported genome Hongyang v4.0,which was assembled with the addition of manual handling.Further evaluation of CentroMiner by searching centromeres in Arabidopsis thaliana and Oryza sativa genomes showed that quarTeT is capable of identifying all the centromeric regions that have been previously detected by experimental methods.Collectively,quarTeT is an efficient toolkit for studies of large-scale T2T genomes and can be accessed at http://gffzz5e5d042d71f64808hb5oxocpx0kbc6vv9.ffgz.tsg.suse.edu.cn/quarTeT/home.html without registration. | Yunzhi Lin Chen Ye Xingzhu Li Qinyao Chen Ying Wu Feng Zhang Rui Pan Sijia Zhang Shuxia Chen Xu Wang Shuo Cao Yingzhen Wang Yi Yue Yongsheng Liu Junyang Yue | 2023 | Horticulture Research2023，10，8： | 0 |
| 9 | Coordination-driven structure reconstruction in polymer of intrinsic microporosity membranes for efficient propylene/propane separation显示文摘Polymers of intrinsic microporosity(PIMs),integrating unique microporous structure and solution-processability,are one class of the most promising membrane materials for energy-efficient gas separations.However,the micropores generated from inefficient chain packing often exhibit wide pore size distribution,making it very challenging to achieve efficient olefin/paraffin separations.Here,we propose a coordination-driven reconstruction(CDR)strategy,where metal ions are incorporated into amidoxime-functionalized PIM-1(AO-PIM)to in situ generate coordination crosslinking networks.By varying the type and content of metal ions,the resulting crosslinking structures can be optimized,and the molecular sieving capability of PIM membranes can be dramatically enhanced.Particularly,the introduction of alkali or alkaline earth metals renders more precise micropores contributing to superior C3H6/C3H8 separation performance.K+incorporated AO-PIM membranes exhibit a high ideal C3H6/C3H8 selectivity of 50,surpassing almost all the reported polymer membranes.Moreover,the coordination crosslinking structure significantly improves the membrane stability under higher pressure as well as the plasticization resistant performance.We envision that this straightforward and generic CDR strategy could potentially unlock the potentials of PIMs for olefin/paraffin separations and many other challenging gas separations. | Yanxiong Ren Boyang Chong Wei Xu Zhengqing Zhang Lin Liu Yingzhen Wu Yutao Liu Haifei Jiang Xu Liang Hong Wu Hongjun Zhang Bangjiao Ye Chongli Zhong Guangwei He Zhongyi Jiang | 2022 | The Innovation2022，3，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员