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| 1 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 2 | β-catenin activation in hair follicle dermal stem cells in duces ectopic hair outgrowth and skin fibrosis显示文摘Hair follicle dermal sheath(DS)harbors hair follicle dermal stem cells(hfDSCs),which can be recruited to replenish DS and dermal papilla(DP).Cultured DS cells can differentiate into various cell lineages in vitro.However,it is unclear how its plasticity is modulated in vivo.Wnt/β-catenin signaling plays an important role in maintaining stem cells of various lineages and is required for HF development and regeneration.Here we report that activation ofβ-catenin in DS generates ectopic HF outgrowth(EF)by reprogramming HF epidermal cells and DS cells themselves,and endows DS cells with hair inducing ability.Epidermal homeostasis of pre-existing HFs is disrupted.Additionally,cell-autonomous progressive skin fibrosis is prominent in dermis,where the excessive fibroblasts largely originate from DS.Gene expression analysis of purified DS cells with activatedβ-catenin revealed significantly increased expression of Bmp,Fgf,and Notch ligands and administration of Bmp,Fgf,or Notch signaling inhibitor attenuates EF formation.In summary,our findings advance the current knowledge of high plasticity of DS cells and provide an insight into understanding how Wnt/β-catenin signaling controls DS cell behaviors. | Yixin Tao Qingchun Yang Lei Wang Jie Zhang Xuming Zhu Qianqian Sun Yunbin Han Qian Luo Yushu Wang Xizhi Guo Ji Wu Baojie Li Xiao Yang Lin He Gang Ma | 2019 | Journal of Molecular Cell Biology2019，11，1： | 4 |
| 3 | Volumetric modulation arc radiotherapy with flattening filter-free beams compared with conventional beams for nasopharyngeal carcinoma:a feasibility study显示文摘There is increasing interest in the clinical use of flattening filter-free(FFF) beams.In this study,we aimed to investigate the dosimetric characteristics of volumetric modulated arc radiotherapy(VMAT) with FFF beams for nasopharyngeal carcinoma(NPC).Ten NPC patients were randomly selected to undergo a RapidArc plan with either FFF beams(RA-FFF) or conventional beams(RA-C).The doses to the planning target volumes(PTVs),organs at risk(OARs),and normal tissues were compared.The technical delivery parameters for RapidArc plans were also assessed to compare the characteristics of FFF and conventional beams.Both techniques delivered adequate doses to PTVs.For PTVs,RA-C delivered lower maximum and mean doses and improved conformity and homogeneity compared with RA-FFF.Both techniques provided similar maximum doses to the optic nerves and lenses.For the brain stem,spinal cord,larynx,parotid glands,oral cavity,and skin,RA-FFF showed significant dose increases compared to RA-C.The dose to normal tissue was lower in RA-FFF.The monitor units(MUs) were(536 ± 46) MU for RA-FFF and(501± 25) MU for RA-C.The treatment duration did not significantly differbetween plans.Although both treatment plans could meet clinical needs,RA-C is dosimetrically superior to RA-FFF for NPC radiotherapy. | Mingzan Zhuang Tuodan Zhang Zhijian Chen Zhixiong Lin Derui Li Xun Peng Qingchun Qiu Renhua Wu | 2013 | Chinese Journal of Cancer2013，32，7： | 3 |
| 4 | Evolution of Neolithic site distribution(9.0–4.0 ka BP) in Anhui,East China显示文摘Based on archaeological surveys of Neolithic cultural development and GIS spatial analysis,this study reproduced the main characteristics of temporal distribution and settlement selection of the sites from the Neolithic Age in Anhui and identified a relationship between environmental evolution and human activity.The results show that altitude,slope direction,and slope gradient were consistent among the settlements at different stages of the Neolithic Age in Anhui,and the sites were mostly distributed in hilly and plain areas on southeast-or south-facing slopes of low gradients close to rivers.We determined that early Neolithic Age(9.0–7.0 ka BP) sites were scattered in small numbers and likely had little cultural exchange with communities of other provinces.The environmental characteristics of various regions in Anhui indicated that the climate was warm and humid with extensive water distribution.The sites of the mid Neolithic Age(7.0–5.0 ka BP) increased rapidly with wide distribution.They were mainly distributed in the plain area north of the Huaihe River and the southwestern areas of Anhui.In the mid Neolithic Age,the warm and humid climate gradually dried,and our ancestors slowly developed cultural exchanges.The largest number of sites existed during the late Neolithic Age(5.0–4.0 ka BP),and were distributed throughout the province.During this period,the overall climate was relatively dry,but humans could still obtain water and other resources through migration.The relatively benign climate facilitated cultural interaction and exchange,which increased during this time,and the Wanjiang culture matured.We also determined that as early civilization evolved,cultures in different regions responded differently to environmental changes.In humid subtropical regions,especially in low-lying plains and areas beside lakes,rivers,and coastal areas,the relatively dry climate in the late period of the middle Holocene,prefaced by a period of high humidity,was conducive to the development of human culture.The evidence from the Neolithic settlements in Anhui therefore reflects this subtropical man-land relationship between cultural development and environmental conditions. | WU Li SUN Xiaoling SUN Wei ZHU Cheng ZHU Tongxin LU Shuguang ZHOU Hui GUO Qingchun GUAN Houchun XIE Wei KE Rui LIN Guiping | 2020 | Journal of Geographical Sciences2020，30，9： | 2 |
| 5 | New lncRNA annotation reveals extensive functional divergence of the transcriptome in maize显示文摘Long non-coding RNAs(lncRNAs), whose sequences are approximately 200 bp or longer and unlikely to encode proteins, may play an important role in eukaryotic gene regulation. Although the latest maize(Zea mays L.) reference genome provides an essential genomic resource, genome-wide annotations of maize lncRNAs have not been updated. Here, we report on a large transcriptomic dataset collected from 749 RNA sequencing experiments across different tissues and stages of the maize reference inbred B73 line and 60 from its wild relative teosinte. We identified 18,165 high-confidence lncRNAs in maize, of which 6,873 are conserved between maize and teosinte. We uncovered distinct genomic characteristics of conserved lncRNAs,non-conserved lncRNAs, and protein-coding transcripts.Intriguingly, Shannon entropy analysis showed that conserved lncRNAs are likely to be expressed similarly to protein-coding transcripts. Co-expression network analysis revealed significant variation in the degree of co-expression. Furthermore, selection analysis indicated that conserved lncRNAs are more likely than non-conserved lncRNAs to be located in regions subject to recent selection, indicating evolutionary differentiation. Our results provide the latest genomewide annotation and analysis of maize lncRNAs and uncover potential functional divergence between proteincoding, conserved lncRNA, and non-conserved lncRNA genes, demonstrating the high complexity of the maize transcriptome. | Linqian Han Zhenna Mu Zi Luo Qingchun Pan Lin Li | 2019 | Journal of Integrative Plant Biology2019，61，4： | 2 |
| 6 | Research and experiments on cutting windows by water-jetting techniques显示文摘 | LIN Fengbo SHANG Qingchun XUE Ling | 2002 | Petroleum Drilling Techniques2002，30，5： | 1 |
| 7 | A New Photonic Bandgap Cover for a Patch Antenna with a Photonic Bandgap Substrate显示文摘 | LIN Qingchun ZHU Fangming HE Sailing | 2004 | Journal of Zhejiang University Science A2004，5，3： | 1 |
| 8 | Generation of gene-target dogs using CRISPR/Cas9 system显示文摘Dear Editor,Dogs(Canis familiaris)serve as human companions and are raised to herd livestock,aid hunters,guard homes,perform police and rescue work,and guide the blind.Dogs exhibit close similarities to humans in terms of metabolic,physiological,and anatomical characteristics,and thus are ideal genetic and clinical models to study human diseases(Tsai et al.,2007).Gene target technology is a powerful tool to create new strains of animals with favorable traits.However,thus far,gene-target dogs have not been developed due to their unique species-specific reproductive characteristics,which limits the applications of dogs especially in the field of biomedical research.Recently,clustered regularly interspaced short palindromic repeats(CRISPRs)/CRISPR-associated(Cas)9 system was applied to edit specific genes with a high efficiency(Cong et al.,2013;Mali et al.,2013).Here we attempt to explore the feasibility of producing gene knockout(KO)dogs by using this technology.Beagle dog,the most widely used breed in biomedical research,was used as our animal model.Myostatin(MSTN)was chosen as the first gene of interest. | Qingjian Zou Xiaomin Wang Yunzhong Liu Zhen Ouyang Haibin Long Shu Wei Jige Xin Bentian Zhao Sisi Lai Jun Shen Qingchun Ni Huaqiang Yang Huilin Zhong Li Li Minhua Hu Quanjun Zhang Zhidong Zhou Jiaxin He Quanmei Yan Nana Fan Yu Zhao Zhaoming Liu Lin Guo Jiao Huang Guanguan Zhang Jun Ying Liangxue Lai Xiang Gao | 2015 | Journal of Molecular Cell Biology2015，7，6： | 1 |
| 9 | A new patch antenna with matamaterial cover显示文摘 | Hu Jun Yan Chunsheng Lin Qingchun | 2006 | Journal of Zhejiang University Science A2006，7，1： | 1 |
| 10 | Improved phase selector for unbalanced faults during power swings using morphological technique显示文摘 | Zou Li Zhao Qingchun Lin Xiangning | 2006 | IEEE Trans on Power Delivery2006，21，4： | 1 |
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