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| 1 | Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis显示文摘An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a 'reporter' gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species. | Junfang Lin Mingyao Zheng Jie Wang Wei Shu Liqiong Guo | 2008 | Progress in Natural Science:Materials International2008，18，7： | 5 |
| 2 | Sputtering Angular Distributions of Individual Elements for Low-Energy Ar Ion Irradiation of PtCu Alloy显示文摘The dynamic Monte Carlo program including a bombardment-induced Gibb-sian segregation process was used to^calculate angular distributions of individual elements from 3putlcrcd Cuq^PIq^alloy in the Ar ion energy range between 0.2 and 1.5ke V.Calculated results show that for each investigated incident energy,the angular distribution of the Pt element is more forward-pointed than that of the Cu one;the reason may be that different percentage of sputtered atoms(37-45% of Pt and 12-19% of Cu)come from beneath the topmost layer. | ZHENG Liping LI Risheng LI Mingyao SHEN Weizu LIN Junying ZOU Zhiyi | 1991 | Chinese Physics Letters1991，8，1： | 2 |
| 3 | Dietary isoleucine improved flesh quality,muscle antioxidant capacity,and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂)显示文摘Background:Muscle is the complex and heterogeneous tissue,which comprises the primary edible part of the trunk of fish and mammals.Previous studies have shown that dietary isoleucine(Ile)exerts beneficial effects on growth in aquatic animals.However,there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle growth.Thus,this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂).Methods:A total of 630 hybrid fish,with an initial average body weight of 33.11±0.09 g,were randomly allotted into seven experimental groups with three replicates each,and respectively fed seven diets with 5.0,7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diets for 8 weeks.Results:In the present study,we demonstrated that Ile significantly:(1)increased muscle protein and lipid contents and the frequency distribution of myofibers with≤20μm and≥50μm of diameter;(2)improved pH value,shear force,cathepsin B and L activities,hydroxyproline content,resilience,cohesiveness,and decreased cooking loss,lactate content,hardness,springiness,gumminess,and chewiness;(3)decreased reactive oxygen species(ROS),malondialdehyde(MDA),and protein carbonyl(PC)contents,GCLC and Keap1 mRNA levels,and up-regulated CuZnSOD,CAT,GPX1a,GST,and Nrf2 mRNA levels;(4)up-regulated the insulin-like growth factor 1,2(IGF-1,IGF-2),insulin-like growth factor 1 receptor(IGF-1R),proliferating cell nuclear antigen(PCNA),Myf5,Myod,Myog,Mrf4,and MyHC mRNA levels,and decreased MSTN mRNAlevel;(5)increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling pathways.Conclusion:These results revealed that dietary Ile improved flesh quality,which might be due to increasing nutritional content,physicochemical,texture parameters,and antioxidant ability;promoting muscle growth by affecting myocytes hyperplasia and hypertrophy,and muscle protein deposition associated with protein synthesis and degradation signaling pathways.Finally,the quadratic regression analysis of chewiness,ROS,and protein contents against dietary Ile levels suggested that the optimal dietary Ile levels for hybrid bagrid catfish was estimated to be 14.19,12.36,and 12.78 g/kg diet,corresponding to 36.59,31.87,and 32.96 g/kg dietary protein,respectively. | Qin Jiang Mingyao Yan Ye Zhao Xiaoqiu Zhou Long Yin Lin Feng Yang Liu Weidan Jiang Pei Wu Yan Wang Defang Chen Shiyong Yang Xiaoli Huang Jun Jiang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 4 | Research on fil- ters for back EMF zero - crossing detecting 显示文摘 | Li Qiang Lin Mingyao Hu Minqiang | 2005 | ICEMS2005，，3： | 1 |
| 5 | Orally administered BZL-s RNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice显示文摘The global COVID-19 pandemic emerged at the end of December 2019.Acute respiratory distress syndrome(ARDS)and acute lung injury(ALI)are common lethal outcomes of bacterial lipopolysaccharide(LPS),avian influenza virus,and SARS-Co V-2.Toll-like receptor 4(TLR4)is a key target in the pathological pathway of ARDS and ALI.Previous studies have reported that herbal small RNAs(s RNAs)are a functional medical component.BZL-s RNA-20(Accession number:B59471456;Family ID:F2201.Q001979.B11)is a potent inhibitor of Toll-like receptor 4(TLR4)and pro-inflammatory cytokines.Furthermore,BZLs RNA-20 reduces intracellular levels of cytokines induced by lipoteichoic acid(LTA)and polyinosinic-polycytidylic acid(poly(I:C)).We found that BZL-s RNA-20 rescued the viability of cells infected with avian influenza H5N1,SARS-Co V-2,and several of its variants of concern(VOCs).Acute lung injury induced by LPS and SARS-Co V-2 in mice was significantly ameliorated by the oral medical decoctosome mimic(bencaosome;sphinganine(d22:0)+BZL-s RNA-20).Our findings suggest that BZLs RNA-20 could be a pan-anti-ARDS/ALI drug. | Dandan Zhao Yuhao Qin Jiaqi Liu Kegong Tang Shuaiyao Lu Zirui Liu Yexuan Lin Cong Zhang Fengming Huang Jiahui Chang Chang Li Mingyao Tian Yiming Ma Xiaoyun Li Congzhao Zhou Xiao Li Xiaozhong Peng Ningyi Jin Chengyu Jiang | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
| 6 | A novel axial field flux-switching permanent magnet wind power generator 显示文摘 | Hao Li Lin Mingyao Li Xin | 2011 | IEEE Transactions on Magnetics2011，47，10： | 1 |
| 7 | Static characteristics analysis and experimental study of a novel axial field flux-switching permanent magnet generator 显示文摘 | Hao Li Lin Mingyao Zhao Xuming | 2012 | IEEE Transactions on Magnetics2012，48，11： | 1 |
| 8 | Novel dual-rotor axial field flux-switching permanent magnet machine 显示文摘 | Hao Li Lin Mingyao Li Wan | 2012 | IEEE Transactions on Magnetics2012，48，11： | 1 |
| 9 | A novel axial field flux-switching permanent magnet wind power generator 显示文摘 | Lin Mingyao Li Hao Li Xin | 2011 | IEEE Transactions on Magnetics2011，47，10： | 1 |
| 10 | Novel dual-rotor axial field flux-switching permanent magnet machine 显示文摘 | Li Hao Lin Mingyao Li Wan | 2012 | IEEE Transactions on Magnetics2012，48，11： | 1 |
| 11 | Development of a ferritin-based nanoparticle vaccine against the SARS-CoV-2 Omicron variant显示文摘Dear Editor,The COVID-19 pandemic has had a devastating effect on global health,resulting in over 6.2 million deaths worldwide.Continuous emergence of adaptive mutations of SARS-CoV-2 alters its pathogenicity and transmissibility,and renders its resistance to current vaccines and antiviral drugs.1 A new variant named Omicron discovered initially in South Africa has recently been proposed as a variant of concern(VOC)by the World Health Organization,because of its high transmissibility and resistance to current vaccines and therapeutic antibodies.2 Therefore,development of vaccines against prevalent variants including Omicron is urgently needed for COVID-19 prevention. | Wanbo Tai Benjie Chai Shengyong Feng Xinyu Zhuang Jun Ma Mujia Pang Lin Pan Zi Yang Mingyao Tian Gong Cheng | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 1 |
| 12 | Effect of Rotor Position Error on Commutation in Sensorless BLDC Motor Drives 显示文摘 | Lin Mingyao Li Qiang Gu Weigang | 2005 | Proceedings of the Eighth International Conference on Electrical Machines and Systems(VolumeI)2005，，1： | 1 |
| 13 | Effect of rotor position error on commutation in sensorless BLDC motor drives显示文摘 | Lin Mingyao Li Qiang Gu Weigang | 2005 | Electrical Machines and Systems2005，，1： | 1 |
| 14 | A novel axial field flux-switching permanent magnet wind power generator 显示文摘 | Li Hao Mingyao Lin Xin Li | 2011 | IEEE Transactions on Magnetics2011，10，47： | 1 |
| 15 | Static characteristics analysis and experimental study of a novel axial field flux-switching permanent magnet generator 显示文摘 | Li Hao Mingyao Lin Xuming Zhao | 2012 | IEEE Transactions on Magnetics2012，11，48： | 1 |
| 16 | Novel Dual-Rotor Axial Field Flux-Switching Permanent Magnet Machine 显示文摘 | Li Hao Mingyao Lin Wan Li | 2012 | IEEE Transactions on Magnetics2012，48，11： | 1 |
| 17 | A Novel Axial Field Flux- Switching Permanent Magnet Wind Power Generator 显示文摘 | Li Hao Mingyao Lin Xin Li etal | 2011 | IEEE Transactions on Magnetics2011，47，10： | 1 |
| 18 | Static Characteristics Analysis and Experimental Study of a Novel Axial Field Flux-Switc- hingPermanent Magnet Generator显示文摘 | Li Hao Mingyao Lin Xuming Zhao | 2012 | IEEE Transactions on Mag- netics2012，48，11： | 1 |
| 19 | Foreign Ownership and International Trade Performance in China显示文摘We investigate the causal relationship between foreign ownership and international trade performance by comparing foreign-acquired firms with similar domestic-acquired firms in China with regard to changes in their post-acquisition international trade performance.Our findings indicate that foreign ownership significantly enhanced the probability of both exporting and importing,and strongly increased trade value.Foreign ownership took effect from the year of the acquisition and persisted for at least 2 years.It stimulated both processing trade and ordinary trade,and expanded products and trading partners.Post-acquisition trade performance also exhibited heterogeneity based on the different pre-acquisition and post-acquisition ownership.As for the underlying mechanisms,we show that firms experienced significant output expansion,increased export dependence,and eased financial constraints after foreign acquisition. | Zhiyuan Li Yichun Lin Mingyao Xu | 2024 | China & World Economy2024，32，2： | 0 |
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