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| 1 | Mortality and Morbidity of Extremely Low Birth Weight Infants in the Mainland of China: A Multi-center Study显示文摘 | Hui-Jia Lin Li-Zhong Du Xiao-Lu Ma Li-Ping Shi Jia-Hua Pan Xiao-Mei Tong Qiu-Ping Li Jian-Guo Zhou Bing Yi Ling Liu Yun-Bing Chen Qiu-Fen Wei Hui-Qing Wu Mei Li Cui-Qing Li Xi-Rong Gao Shi-Wen Xia Wen-Bin Li Chao-Ying Ya Ling He Kun Liang Xiao-Yu Zhou Shu-Ping Han Qin Lyu Yin-Ping Qiu Wen Li Dong-Mei Chen Hong-Ru Lu Xiao-Hong Liu Hong Liu Zhen-Lang Lin Li Liu Jia-Jun Zhu Hong Xiong Shao-Jie Yue Si-Qi Zhuang | 2015 | Chinese Medical Journal2015，，20： | 49 |
| 2 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 3 | Rhubarb Monomers Protect Intestinal Mucosal Barrier in Sepsis via Junction Proteins显示文摘 | Lyu Wang Yun-Liang Cui Zhe Zhang Zhao-Fen Lin De-Chang Chen | 2017 | Chinese Medical Journal2017，，10： | 23 |
| 4 | Delirium in Intensive Care Unit Patients: Ten Important Points of Understanding显示文摘 | Xiao-Ting Wang Lin Lyu Bo Tang Cui Wang Da-Wei Liu | 2017 | Chinese Medical Journal2017，，20： | 17 |
| 5 | Health Effect of Forest Bathing Trip on Elderly Patients with Chronic Obstructive Pulmonary Disease显示文摘Forest bathing trip is a short,leisurely visit to forest.In this study we determined the health effects of forest bathing trip on elderly patients with chronic obstructive pulmonary disease(COPD).The patients were randomly divided into two groups.One group was sent to forest,and the other was sent to an urban area as control. | JIA Bing Bing YANG Zhou Xin MAO Gen Xiang LYU Yuan Dong WEN Xiao Lin XU Wei Hong LYU XIAO Ling CAO Yong Bao WANG Guo Fu | 2016 | Biomedical and Environmental Sciences2016，29，3： | 15 |
| 6 | Effects of Crude Oil Contamination on Soil Physical and Chemical Properties in Momoge Wetland of China显示文摘Large oilfields are often coincidentally located in major river deltas and wetlands,and potentially damage the structure,function and ecosystem service values of wetlands during oil exploration.In the present study,the effects of crude oil contamination during oil exploration on soil physical and chemical properties were investigated in marshes of the Momoge National Nature Reserve in Jilin Province,China.The concentrations of total petroleum hydrocarbons in the marsh soil near the oil wells are significantly higher than those in the adjacent control marsh.Soil water contents in oil-contaminated marshes are negatively correlated with soil temperature and are significantly lower than those in the control area,especially in fall.Crude oil contamination significantly increases the soil pH up to8.0,and reduces available phosphorus concentrations in the soil.The concentrations of total organic carbon are significantly different among sampling sites.Therefore,crude oil contamination could potentially alkalinize marsh soils,adversely affect soil fertility and physical properties,and cause deterioration of the marshes in the Momoge National Nature Reserve.Phyto-remediation by planting Calamagrostis angustifolia has the potential to simultaneously restore and remediate the petroleum hydrocarbon-contaminated wetlands.Crude oil contamination affects the soil physical and chemical properties,so developing an effective restoration program in the Momoge wetland is neccesary. | WANG Ying FENG Jiang LIN Qianxin LYU Xianguo WANG Xiaoyu WANG Guoping | 2013 | Chinese Geographical Science2013，23，6： | 12 |
| 7 | Long noncoding RNAs in neurodevelopment and Parkinson’s disease显示文摘Long noncoding RNAs(lnc RNAs) are RNA molecules comprising more than 200 nucleotides, which are not translated into proteins. Many studies have shown that lnc RNAs are involved in regulating a variety of biological processes, including immune, cancer, stress, development and differentiation at the transcriptional, epigenetic or post-transcriptional levels. Here, we review the role of lnc RNAs in the process of neurodevelopment, neural differentiation, synaptic function, and pathogenesis of Parkinson’s disease(PD). These pathomechanisms include protein misfolding and aggregation, disordered protein degradation, mitochondrial dysfunction, oxidative stress, autophagy, apoptosis, and neuroinflammation. This information will provide the basis of lnc RNA-based disease diagnosis and drug treatment for PD. | Ying Lyu Lin Bai Chuan Qin | 2019 | Animal Models and Experimental Medicine2019，2，4： | 10 |
| 8 | First proof-of-principle experiment with the post-accelerated isotope separator on-line beam at BRIF:measurement of the angular distribution of ^(23)Na+^(40)Ca elastic scattering显示文摘The reaction dynamics of exotic nuclei near the drip line is one of the main research topics of current interest.Elastic scattering is a useful probe for investigating the size and surface diffuseness of exotic nuclei.The development of rare isotope accelerators offers opportunities for such studies.To date,many relevant measurements have been performed at accelerators using the projectile fragmentation technique,while the measurements at accelerators using isotope separator on-line(ISOL)systems are still quite scarce.In this work,we present the first proof-of-principle experiment with a post-accelerated ISOL beam at the Beijing Radioactive Ion Beam Facility(BRIF)by measuring the angular distribution of elastic scattering for the stable nucleus^(23)Na from the doubly magic nucleus^(40)Ca at energies above the Coulomb barrier.The angular distribution measured by a silicon strip detector array in a scattering chamber using the ISOL beam at BRIF is in good agreement with that measured by the high-precision Q3 D magnetic spectrograph using the nonISOL beam at nearly the same energy.This work provides useful background for making BRIF a powerful tool for the investigation of the reaction dynamics of exotic nuclei. | Wei Nan Bing Guo Cheng-Jian Lin Lei Yang Dong-Xi Wang Yang-Ping Shen Bing Tang Bao-Qun Cui Tao Ge Yin-Long Lyu Hui-Ming Jia Yun-Ju Li Chen Chen Li-Hua Chen Qi-Wen Fan Xin-Yue Li Gang Lian Jian-Cheng Liu Tian-Peng Luo Nan-Ru Ma Rui-Gang Ma Xie Ma Ying-Jun Ma Wei-Ke Nan Dan-Yang Pang You-Bao Wang Pei-Wei Wen Feng Yang Yong-Jin Yao Sheng Zeng Hao Zhang Fu-Peng Zhong Shan-Hao Zhong Zhi-Hong Li Tian-Jue Zhang Wei-Ping Liu BRIF Collaboration | 2021 | Nuclear Science and Techniques2021，32，5： | 9 |
| 9 | Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration显示文摘The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro.However,conventional organoid cultures primarily represent the homeostasis of self-organizing stem cells and their derivatives.Here,we established a novel intestinal organoid culture system composed of 8 components,mainly including VPA,EPZ6438,LDN193189,and R-Spondin 1 conditioned medium,which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury;therefore,these organoids were designated hyperplastic intestinal organoids(Hyper-organoids).Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5+stem cells or Clu+revival stem cells.Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids,which functioned through epigenetically regulating YAP signaling.Furthermore,VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo.In summary,our results demonstrated a new in vitro organoid model to study epithelial regeneration,highlighting the importance of epigenetic reprogramming that pioneers tissue repair. | Molong Qu Liang Xiong Yulin Lyu Xiannian Zhang Jie Shen Jingyang Guan Peiyuan Chai Zhongqing Lin Boyao Nie Cheng Li Jun Xu Hongkui Deng | 2021 | Cell Research2021，31，3： | 9 |
| 10 | Analysis of Health Service Utilization and its Influencing Factors among Patients with Pneumoconiosis in China显示文摘Pneumoconiosis,an interstitial lung disease that occurs from breathing in certain kinds of damaging dust particles,is a major occupational disease in China.Patients diagnosed with occupational pneumoconiosis can avail of free medical treatment,whereas patients without a diagnosis of occupational diseases cannot not claim free medical treatment in most provinces from the government before 2019.This study aimed to analyze the priority of medical facility selection and its influencing factors among patients with pneumoconiosis. | WANG Huan Qiang JIA Jun Lin JIANG Zhao Qiang JIN Qi LI Dong Xia LING Rui Jie LI Ying CUI Ping CHEN Gang ZHAO Hong Yu LI Yan WEN Ke LYU Xiang Pei LOU Jian Lin LI Tao | 2021 | Biomedical and Environmental Sciences2021，34，1： | 9 |
| 11 | Neonatal ketamine exposure-induced hippocampal neuroapoptosis in the developing brain impairs adult spatial learning ability显示文摘Ketamine exposure can lead to selective neuroapoptosis in the developing brain.p66ShcA,the cellular adapter protein expressed selectively in immature neurons,is a known pro-apoptotic molecule that triggers neuroapoptosis when activated.Sprague-Dawley rats at postnatal day 7 were subcutaneously injected in the neck with ketamine 20 mg/kg,six times at 2-hour intervals.At 0,1,3,and 6 hours after final injection,western blot assay was used to detect the expression of cleaved caspase-3,p66ShcA,and phosphorylated p66ShcA.We found that the expression of activated p66ShcA and caspase-3 increased after ketamine exposure and peaked at 3 hours.The same procedure was performed on a different group of rats.At the age of 4 weeks,spatial learning and memory abilities were tested with the Morris water maze.Latency to find the hidden platform for these rats was longer than it was for control rats,although the residence time in the target quadrant was similar.These findings indicate that ketamine exposure resulted in p66ShcA being activated in the course of an apoptotic cascade during the neonatal period.This may have contributed to the deficit in spatial learning and memory that persisted into adulthood.The experimental protocol was approved by the Institutional Animal Care and Use Committee at the University of Texas at Arlington,USA (approval No.A13.008) on January 22,2013. | Dan Lyu Ning Tang Andrew W. Womack Yong-Jin He Qing Lin | 2020 | Neural Regeneration Research2020，15，5： | 7 |
| 12 | Association between Perceived Stressfulness of Stressful Life Events and the Suicidal Risk in Chinese Patients with Major Depressive Disorder显示文摘 | Jing-Yu Lin Yu Huang Yun-Ai Su Xin Yu Xiao-Zhen Lyu Qi Liu Tian-Mei Si | 2018 | Chinese Medical Journal2018，，8： | 7 |
| 13 | Effect of Weight Loss on Survival in Esophageal Cancer Patients undergoing Neoadjuvant Chemoradiotherapy and Surgery显示文摘Objective This study aimed to determine the impact of weight loss during neoadjuvant chemoradiotherapy on the survival of patients with esophageal carcinoma.Methods We retrospectively examined 102 consecutive patients with esophageal carcinoma who underwent neoadjuvant chemoradiotherapy followed by radical resection at Sichuan Cancer Hospital&Institute between 2003 and 2017.The patients were divided into three groups based on the amount of body weight lost during neoadjuvant chemoradiotherapy:severe weight loss(>10%),high weight loss(5%~10%),and low weight loss(<5%).The correlations of weight loss with toxicity,progressionfree survival,and overall survival were investigated.Results The median overall survival was 49.7 months in the low weight loss group compared with 35.4 and 25.1 months in the high and severe weight loss groups(P=0.041).The 1-year overall survival rates in the severe,high,and low weight loss groups were 62.5%,85.0%,and 90.7%,respectively;the corresponding 3-year overall survival rates were 21.9%,47.3%,and 68.8%,respectively,and the corresponding 5-year overall survival rates were 21.9%,31.0%,and 44.4%,respectively.The multivariate analysis indicated that a pathological complete response and severe weight loss were independent prognostic factors for overall survival.Any leukopenia(P=0.024),leukopenia of at least grade 3(P=0.021),and anemia(P=0.042)occurred more frequently in the severe weight loss group.Conclusions Weight loss during neoadjuvant CRT is an independent and adverse prognostic factor in esophageal carcinoma patients,whereas a stable weight confers a better prognosis. | Jia Hua Lyu Tao Li Yong Tao Han Lei Wu Lin Peng Qi Feng Wang Long Liang | 2020 | Journal of Nutritional Oncology2020，5，3： | 6 |
| 14 | Monoclonal neutralizing antibodies against EV71 screened from mice immunized with yeast-produced virus-like particles显示文摘Periodic outbreaks of hand, foot and mouth disease(HFMD) occur in children under 5 years old, and can cause death in some cases. The C4 strain of enterovirus 71(EV71) is the main pathogen that causes HFMD in China. Although no drugs against EV71 are available, some studies have shown that candidate vaccines or viral capsid proteins can produce anti-EV71 immunity. In this study, female BABL/c mice(6–8 weeks old) were immunized with virus-like particles(VLPs) of EV71 produced in yeast to screen for anti-EV71 antibodies. Two hybridomas that could produce neutralizing antibodies against EV71 were obtained. Both neutralizing m Abs(D4 and G12) were confirmed to bind the VP1 capsid protein of EV71, and could protect > 95% cells from 100 TCID50 EV71 infection at 25 μg/m L solution(lowest concentration). Those two neutralizing m Abs identified in the study may be promising candidates in development for m Abs to treat EV71 infection, and utilized as suitable reagents for use in diagnostic tests and biological studies. | Tao Lin Lingzhi Xianyu Songya Lyu | 2015 | Virologica Sinica2015，30，3： | 6 |
| 15 | Key determinants of misdiagnosis of tracheobronchial tuberculosis among senile patients in contemporary clinical practice:A retrospective analysis显示文摘BACKGROUND Tracheobronchial tuberculosis(TBTB)is a common subtype of pulmonary tuberculosis.Concomitant diseases often obscure the diagnosis of senile TBTB.AIM To characterize senile patients with TBTB and to identify the potential causes of misdiagnosis.METHODS One hundred twenty patients with senile TBTB who were admitted to the Anhui Chest hospital between May 2017 and May 2019 were retrospectively analyzed.Patients were classified as diagnosed group(n=58)and misdiagnosed group(n=62).Clinical manifestations,laboratory results,radiographic data,and endoscopic findings were compared between the two groups.RESULTS Patients in the misdiagnosed group were most commonly diagnosed as pulmonary tuberculosis(non-TBTB,29/62,46.8%),general pneumonia(9/62,14.5%),chronic obstructive pulmonary disease(8/62,12.9%),and tracheobronchial carcinoma(7/62,11.3%).The time elapsed between disease onset and confirmation of diagnosis was significantly longer in the misdiagnosed group[median(first quartile,third quartile):6.32(4.94,16.02)mo vs 3.73(2.37,8.52)mo].The misdiagnosed group had lower proportion of patients who underwent bronchoscopy[33.87%(21/62)vs 87.93%(51/58)],chest computed tomography(CT)scan[69.35%(43/62)vs 98.28%(57/58)],and those who showed CT signs of tuberculosis[27.91%(12/62)vs 50%(29/58)]as compared to that in the diagnosed group(P<0.05).There were no significant between-group differences with respect to age,gender,occupation,clinical manifestations,or prevalence of comorbid chronic diseases(P>0.05).CONCLUSION Insufficient or inaccurate radiographic or bronchoscopic assessment was the predominant cause of delayed diagnosis of TBTB.Increased implementation and better interpretation of CT scan and early implementation of bronchoscopy can help reduce misdiagnosis of senile TBTB. | Fei Tang Lian-Jun Lin Shu-Liang Guo Wei Ye Xian-Kui Zha Yu Cheng Ying-Feng Wu Yue-Ming Wang Xiao-Mei Lyu Xiao-Yun Fan Li-Ping Lyu | 2021 | World Journal of Clinical Cases2021，9，25： | 6 |
| 16 | TCP7 functions redundantly with several Class Ⅰ TCPs and regulates endoreplication in Arabidopsis显示文摘TCP(TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR) proteins, a family of plantspecific transcription factors, play important roles in many developmental processes. However, genetic and functional redundancy among class I TCP limits the analysis of their biological roles. Here, we identified a dominantnegative mutant of Arabidopsis thaliana TCP7 named leaf curling-upward(lcu), which exhibits smaller leaf cells and shorter hypocotyls than the wild type, due to defective endoreplication. A septuple loss-of-function mutant of TCP7, TCP8, TCP14, TCP15, TCP21, TCP22, and TCP23 displayed similar developmental defects to those of lcu.Genome-wide RNA-sequencing showed that lcu and the septuple mutant share many misexpressed genes.Intriguingly, TCP7 directly targets the CYCLIN D1;1 (CYCD1;1) locus and activates its transcription. We determined that the C-terminus of TCP7 accounts for its transcriptional activation activity. Furthermore, the mutant protein LCU exhibited reduced transcriptional activation activity due to the introduction of an EAR-like repressive domain at its C-terminus. Together, these observations indicate that TCP7 plays important roles during leaf and hypocotyl development, redundantly, with at least six class I TCPs, and regulates the expression of CYCD1;1 to affect endoreplication in Arabidopsis. | Guofang Zhang Hongtao Zhao Chunguang Zhang Xiaoyun Li Yuanyuan Lyu Dongmei Qi Yanwei Cui Lin Hu Zhenjie Wang Zheng Liang Sujuan Cui | 2019 | Journal of Integrative Plant Biology2019，61，11： | 5 |
| 17 | Cytogenetic biodosimetry for radiation accidents in China显示文摘Cytogenetic indicators,especially dicentric chromosomes,have been globally considered the gold standard of biological dose estimation for more than 60 years.The application of these indicators to biological dose estimation in victims of radiation accidents in China has undergone approximately 40 years of development and thus provides more accurate information for the clinical diagnosis and patients’treatment.This paper reviews the application and future development of cytogenetic indicators,including chromosomal aberrations(dicentric chromosomes and chromosomal translocations),cytokinesis-block micronuclei,nucleoplasmic bridges,and premature condensation chromosome ring,as biodosimeters in China. | Lin Han Yu Gao Ping Wang Yumin Lyu | 2020 | Radiation Medicine and Protection2020，1，3： | 4 |
| 18 | Characteristic model based all-coefficient adaptive control of an AMB suspended energy storage flywheel test rig显示文摘Feedback control of active magnetic bearing(AMB) suspended energy storage flywheel systems is critical in the operation of the systems and has been well studied. Both the classical proportional-integralderivative(PID) control design method and modern control theory, such as H∞ control and μ-synthesis,have been explored. PID control is easy to implement but is not effective in handling complex rotordynamics. Modern control design methods usually require a plant model and an accurate characterization of the uncertainties. In each case, few experimental validation results on the closed-loop performance are available because of the costs and the technical challenges associated with the construction of experimental test rigs.In this paper, we apply the characteristic model based all-coefficient adaptive control(ACAC) design method for the stabilization of an AMB suspended flywheel test rig we recently constructed. Both simulation and experimental results demonstrate strong closed-loop performance in spite of the simplicity of the control design and implementation. | Xujun LYU Long DI Zongli LIN Yefa HU Huachun WU | 2018 | Science China(Information Sciences)2018，61，11： | 4 |
| 19 | Long-term variations of major atmospheric compositions observed at the background stations in three key areas of China显示文摘Atmospheric compositions and acid rain are closely related to atmospheric environment,weather,and climate change.In this study,the concentration levels and long-term trends of CO_(2),CH_(4),O_(3),PM_(2.5),PM_(10),and acid rain were presented at the Mt.Waliguan global baseline station in Qinghai,Shangdianzi and Lin'an regional background stations in the North China Plain(NCP)and the Yangtze River Delta region(YRD)of China based on the data observed until 2018.Significant increasing trends of CO_(2) were found at all of these stations.At Mt.Waliguan,the concentration levels and growth rate were consistent with the average values in the Northern Hemisphere(NH)and those observed at the Mauna Loa station in Hawaii.The CH_(4) also showed a upward tendency similar to that observed in NH.Compared with the observations at Mt.Waliguan global baseline station,the CO_(2) and CH_(4) mole fractions were higher at Shangdianzi and Lin'an regional background stations,indicating the important contributions of anthropogenic activities to the regional background level of CO_(2) and CH_(4).The annual averaged surface ozone showed increasing trends at Mt.Waliguan and Shangdianzi stations,whereas no clear trend was found at Lin'an station.Similar variations in the patterns of aerosol concentrations at Shangdianzi and Lin'an were found according to the PM_(2.5) or PM_(10) observations.Overall,the aerosol concentrations at both stations shown declining trends since the observations.However,two periods of high particle concentrations occurred in 2006-2007 and 2013-2014.The annual mean aerosol concentrations have continuously decreased after 2013-2014,indicating the effectiveness of pollution control in the NCP and YRD,especially since the implementation of the‘Action Plan for Prevention and Control of Air Pollution’in September 2013.Comparative analysis of aerosol with acid rain showed that the period with high concentrations of aerosols in the early years was consistent with that of severe acid rain pollution,suggesting that both aerosol pollution and severe acid rain are closely related to coal combustion emissions in China.As a result of the control of coal combustion emissions for acid and SO_(2),NO_(x) emissions have contributed more substantially to aerosols due to the rapid growth of vehicle ownership.A comparison of the trends of aerosols with the maximum daily 8-h average of surface ozone(MDA8)at Shangdianzi and Lin'an stations revealed the complicated relationship between aerosol reduction and ozone production,i.e.,together with the continuous improvement of particulate pollution in the NCP and YRD,the MDA8 at both stations showed a decreasing trend at first but subsequently increased after 2014 with aerosols remaining reduced.Such different variation patterns of the MDA8 with PM_(2.5)(or PM_(10))indicated the high challenge of synergic control of aerosol and ozone pollution in China. | ZHANG Yong JIN Jun-Li YAN Peng TANG Jie FANG Shuang-Xi LIN Wei-Li LOU Meng-Yun LIANG Miao ZHOU Qing JING Jun-Shan LI Ya-Nan JIA Xiao-Fang LYU Shan-Shan | 2020 | Advances in Climate Change Research2020，11，4： | 4 |
| 20 | Longitudinal analysis reveals characteristically high proportions of bacterial vaginosis-associated bacteria and temporal variability of vaginal microbiota in northern pig-tailed macaques (Macaca leonina)显示文摘The complex and dynamic vaginal microbial ecosystem is critical to both health and disease of the host.Studies focusing on how vaginal microbiota influences HIV-1 infection may face limitations in selecting proper animal models.Given that northern pig-tailed macaques(Macaca leonina) are susceptible to HIV-1 infection,they may be an optimal animal model for elucidating the mechanisms by which vaginal microbiota contributes to resistance and susceptibility to HIV-1 infection.However,little is known about the composition and temporal variability of vaginal microbiota of the northern pig-tailed macaque.Here,we present a comprehensive catalog of the composition and temporal dynamics of vaginal microbiota of two healthy northern pig-tailed macaques over 19 weeks using 454-pyrosequencing of 16 S rRNA genes.We found remarkably high proportions of a diverse array of anaerobic bacteria associated with bacterial vaginosis.Atopobium and Sneathia were dominant genera,and interestingly,we demonstrated the presence of Lactobacillus-dommateti vaginal microbiota.Moreover,longitudinal analysis demonstrated that the temporal dynamics of the vaginal microbiota were considerably individualized.Finally,network analysis revealed that vaginal pH may influence the temporal dynamics of the vaginal microbiota,suggesting that inter-subject variability of vaginal bacterial communities could be mirrored in inter-subject variation in correlation profiles of species with each other and with vaginal pH over time.Our results suggest that the northern pig-tailed macaque could be an ideal animal model for prospective investigation of the mechanisms by which vaginal microbiota influence susceptibility and resistance to HIV-1 infection in the context of highly poiymicrobiai and Lactobacillus-dommated states. | Lin ZHU Ai-Hua LEI Hong-Yi ZHENG Long-Bao LYU Zhi-Gang ZHANG Yong-Tang ZHENG | 2015 | Zoological Research2015，36，5： | 4 |
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