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| 1 | The cloning of Dmc1 cDNAs and a comparative study of its expression in different ploidy cyprinid fishes显示文摘Dmc1 (disrupted meiotic cDNA) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. In this investigation, we cloned the partial cDNAs of Dmc1 of diploid red crucian carp, Japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the DMC1 protein in yeast, mouse and human. The full length cDNAs were then obtained by rapid amplification of cDNA ends (RACE). Our data showed that the full length cDNAs of Dmc1 in the three diploid fishes are all 1375 bp long, while it is 1383 bp long in triploids and 1379 bp long in allotetraploids. And despite of the variation in length, all the cDNAs encode a protein of 342 amino acids. A high homology of 97.3% of the DMC1 protein can be drawn by comparing the amino acid sequences in the three diploids, which is also of 86%, 86% and 95% similarity to human, mouse and zebrafish, respectively. A comparative study of the expression pattern of Dmc1 was carried out by RT-PCR using specific primers against the same se-quences of coding regions in different ploidy cyprinid fishes, from which it was showed that Dmc1 was expressed only in gonads of these five kinds of fishes. The expression pattern of Dmc1 in both ovaries and testes from different ploidy fishes within breeding season was also studied by Real-time PCR, and the results showed that the expression of this gene was greatly different among the three different ploidy fishes, which was the highest of triploid and lowest of allotetraploids. The histological sections data showed matured gonads of both diploid red crucian carp and allotetraploids in breeding season, although the latter demonstrated a higher maturation, and no gonadal maturation could be observed in triploids. In conclusion, we suggest that Dmc1 is specifically expressed in the period of meiosis in all the ploidy cyprinid fishes and directly related with the development of gonad in a manner of ploidy-independent way. And further, the high expression of Dmc1 in female triploids might be associ-ated with abnormal meiosis and sterility. | TAO Min, LIU ShaoJun, LONG Yu, ZENG Chen, LIU JiFang, LIU LiangGuo, ZHANG Chun, DUAN Wei & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，1： | 13 |
| 2 | Evolutionary analysis of allotetraploid hybrids of red crucian carp×common carp,based on ISSR,AFLP molecular markers and cloning of cyclins genes显示文摘The allotetraploid hybrids of red crucian carp × common carp are the first reported artificially cultured polyploid fish with bisexual fertility and stable inheritance in vertebrate. Using ISSR and AFLP markers and the cyclins genes, the genomes and cyclin gene sequence changes were analyzed between the allotetraploid hybrids and their parents. The results indicated that the allotetraploids inherited many genetic characteristics from their parents and the genetic characteristics were stable after 15 generations. However, the allotetraploids had a closer genetic relationship with their original female parents and represented a bias toward the maternal progenitor. DNA fingerprinting analysis showed that the allotetraploids had undergone sequences deletion from their original parents and that the deleted sequences were mostly from the male parent's genome. Some non-parental bands were found in the allotetraploid hybrids. Sequences analysis of the cyclin A1 and B1 genes showed nonsynonymous substitutions of single nucleotides in codons that were different from their original parents, leading to non-parental amino acid loci. We speculate that the non-additivity in the allotetraploids, compared with their progenitors, could be an adjustment to the genomic shock from heterozygosity and polyploidy, allowing maintenance of genetic stability. | LIU LiangGuo YAN JinPeng LIU ShaoJun LIU Dong YOU CuiPing ZHONG Huan TAO Min LIU Yun | 2009 | Chinese Science Bulletin2009，54，16： | 6 |
| 3 | Influence of wave front healing on seismic tomography显示文摘Wave front healing is a common natural phenomenon.To further investigate wave front healing,we simulated wave propagation in a spherical anomaly surrounded by homogeneous media using a high-order finite difference solution of the acoustic equation.Furthermore,we analyzed the characteristics of the wave propagation in the anomaly,and found that they are related to the dominant frequency of the seismic wave and the dimensions of the anomaly.Through quantitative comparison of the wave front energy of the diffracted wave and transmitted wave,we summarized the influences of the wave front healing on seismic tomography.We conclude that,under the strong scattering condition,only positive anomalies can be inverted by ray-based tomography,only large anomalies can be inverted by finite-frequency tomography,and small negative anomalies cannot be inverted by any first-arrival traveltime tomographic methods.These conclusions are verified by tomographic experiments based on different theoretical models.Finally,we propose that more information besides the first-arrival traveltime should be used to invert the high wave number components of the media.Besides the above acquisitions of wave front healing on seismic tomography,we explain the banana-doughnut phenomena,and offer a new insight into the wave scattering,which should be important for better understanding the wave propagation and seismic inversion. | Liu YuZhu Dong LiangGuo | 2012 | Science China Earth Sciences2012，55，11： | 3 |
| 4 | Cloning of a novel gene associated with human nasopharyngeal carcinoma显示文摘One EST N27741 with high expression in normal adult nasopharynx tissues but low expression in adult poorly differentiated squamous nasopharyngeal carcinoma has been selected out by the high-density cDNA array expression profiling technique. The differential expression has been confirmed by RT-PCR. One novel gene of 1096 bp has been cloned based on this EST. Bioinformatics analysis found that the new gene sequence contains a whole reading frame encoding 256 amino acids. There is a stop codon TAA in front of the 5’ end start codon, and a tailing signal AATAAA and poly A tail at the 3’ end. There is no homologous known gene found after searching by blasting this sequence to non-redundancy nucleotide database. Therefore it is considered a novel gene related to nasopharyngeal carcinoma. | Zhiwei He Lu Xie Liangguo Xu Ke Lan Weidong Liu Ling Zhang Caiping Ren Jianling Shi Wen Zhou Kaitai Yao | 2000 | Chinese Science Bulletin2000，45，24： | 2 |
| 5 | Recombinant soluble TRAIL induces apoptosis of cancer cells显示文摘TRAIL is a tumor necrosis factor family member that selectively induces apoptosis of cancer cells but not of normal cells. To develop TRAIL into a potential cancer drug, three different sizes of soluble TRAIL fragments, including sTRAIL(74 - 281), sTRAIL(95 - 281) and sTRAIL(101-281), were expressed in E. coli and purified to homogeneity. Apoptosis assays indicated that sTRAIL(95-281) and sTRAIL(101-281), but not sTRAIL(74-281), can potently induce apoptosis of various cancer cell lines in 6 h, suggesting that the N-terminal fragment of aa101 has inhibitory effect on TRAIL-induced apoptosis. Moreover, we found that some cancer cells were resistant to TRAIL and the resistant cells could be converted into sensitive cells by treatment with the protein synthesis inhibitor cycloheximide, suggesting that one or more short-lived proteins are responsible for cells’ resistance to TRAIL. | LI Lianyun HU Jiancheng BIN Lianghua LI Lixia ZHA Jikun XU Liangguo ZHANG Zhen ZHAI Zhonghe SHU Hongbing | 2001 | Chinese Science Bulletin2001，46，24： | 2 |
| 6 | abrication of CDs hybrid MIL-68(In) derived In2O_(3)eIn_(2)S_(3) hollow tubular heterojunction and their exceptional self-powered PEC aptasensing properties for ampicillin detecting显示文摘A visible-light-driven self-powered photoelectrochemical(PEC)aptasensor was developed for ampicillin(AMP)detecting based on carbon dots(CDs)hybrid MIL-68(In)-derived In2O_(3)eIn_(2)S_(3) hollow tubular heterojunction(CDs/In2O_(3)eIn_(2)S_(3)).In_(2)S_(3) nanosheets uniformly grew in-situ on the surface of In2O_(3) hollow tubes,forming a close contact heterogeneous interface,which significantly promoted the transfer and separation of photo-generated carriers,and provided a large specific surface area and rich active sites for the PEC aptasensing platform.Furthermore,the CDs/In2O_(3)eIn_(2)S_(3)/ITO electrode,which was obtained by dipping assembly,showed remarkable and stable photoelectric signals at zero-bias under visible light irradiation.The amino-functionalized AMP aptamer was fixed on the working electrode as a biological recognition element,and the concentration of AMP was determined by observing the change in photocurrent intensity caused by the specific capture of AMP molecules in solution.Under optimized conditions,the developed PEC aptasensor displayed a relatively wide linear range from 0.001 ng mL^(-1) to 300 ng mL^(-1),as well as a low detection limit(LOD)of 0.06 pg mL^(-1).Besides,the novel self-powered PEC AMP-aptasensor exhibited excellent reproducibility,good stability and selectivity,which open a potential avenue for antibiotic residues detection in environmental media. | Tao Yan Yixuan Feng Xiang Ren Jinkai Li Yizhong Lu Meng Sun Liangguo Yan Qin Wei Huangxian Ju | 2021 | Journal of Materiomics2021，7，4： | 2 |
| 7 | Profiling gene expression patterns of nasopharyngeal carcinoma and normal nasopharynx tissues with cDNA microarray显示文摘5 μg of total RNAs from normal nasopharynx and nasopharyngeal carcinoma tissue have been labeled with α-32P-dCTP during reverse transcription. The synthesized cDNA probes have been hybridized to high-density cDNA microarray containing 5184 genes or expression sequence tags (ESTs). Then image analysis software has been applied to comparing their expression profiles. Results show that 187 ESTs were of density value above 200 in nasopharyngeal carcinoma tissue while there were 307 such ESTs in normal nasopharynx tissue; 38 ESTs were strongly expressed in nasopharynx, but weakly expressed in nasopharyngeal carcinoma; 48 ESTs were strongly expressed in nasopharyngeal carcinoma, but weakly expressed in normal nasopharynx. These results suggest that there may exist some new differentially expressed genes involved in nasopharyngeal carcinoma development. Furthermore, the results strongly indicate that high-density cDNA microarray is a powerful and efficient tool for large-scale screening differentially | Zhiwei He Caiping Ren Liangguo Xu Weidong Liu Lu Xie Zhongkui Li Kaitai Yao | 2000 | Chinese Science Bulletin2000，45，9： | 2 |
| 8 | Comparative analysis and evolutionary significance of the HMG1 gene in crucian carp,blunt snout bream,and their polyploid progeny显示文摘高活动性组蛋白质的全身的 mRNA 1 个编码基因(HMG1 ) 被 RACE-PCR 从红鲫鱼鲤鱼(Carassius auratus 红 var ) 获得,钝猪嘴鲷(Megalobrama amblycephala ) ,并且他们的三倍性并且 tetraploid 子孙。顺序包含了为 193 氨基酸编码的 579 核苷酸的一个开的读物框架。HMG1 的核苷酸身份比在 tetraploid 混血儿和父亲的钝猪嘴鲷(97%) 之间在 tetraploid 混血儿和母亲的红鲫鱼鲤鱼(99%) 之间是更高的。在三倍性的混血儿和每个父母(95%) 之间的核苷酸身份在父母(98%) 之间是比那低的。在 tetraploid 混血儿和每个父母(100%) 之间的蛋白质身份在三倍性的混血儿和每个父母(97%) 之间比那高。我们的结果建议种间的杂交在三倍性的混血儿产生震动到 HMG1 基因,引起核苷酸的分叉。tetraploid 混血儿的 HMG1 蛋白质与它的父母的一致,它减少了在等位基因之间的生气不兼容的障碍,提供形成在自然的新多倍体种的两性的肥沃的 tetraploid 混血儿的基础。HMG1 蛋白质的第二等、第三级的结构包含八他 lices,三个开关,在 N 终点的二个 DNA 有约束力的领域,和在 C 终点的一条长酸的尾巴。一起,这些数据建议 HMG1 蛋白质起蛋白质 DNA 相互作用的一个作用,在原子核便于各种各样的 DNA 依赖的活动。我们也调查了鱼,两栖动物,爬虫,鸟,和哺乳动物的 HMG1 蛋白质的发展史。我们的结果建议 HMG1 是高度被保存了的祖先的蛋白质。这些数据提供种间的杂交怎么可以形成多倍体混血儿的线索。 | Dong Liu Zhen Liu Shaojun Liu Liangguo Liu Cuiping You Lin Chen Huan Zhong Yun Liu | 2009 | Progress in Natural Science:Materials International2009，19，12： | 1 |
| 9 | Adsorption of benzoic acid from aqueous solution by three kinds of modified bentonites显示文摘 | Xiaodong Xin Wei Si Zhenxing Yao Rui Feng Bin Du Liangguo Yan Qin Wei | 2011 | Journal of Colloid And Interface Science2011，，2： | 1 |
| 10 | Identification of differentially expressed genes in nasopharyngeal carcinoma by means of the Atlas human cancer cDNA expression array显示文摘 | Xie Lu Xu Liangguo He Zhiwei | 2000 | J Cancer Res Clin Oncol2000，126，： | 1 |
| 11 | Crosslinking acrylamide with EDTA-intercalated layered double hydroxide for enhanced recovery of Cr(Ⅵ)and Congo red:Adsorptive and mechanistic study显示文摘We prepared ethylenediaminetetraacetic acid(EDTA)-intercalated MgAl-layered double hydroxide(LDH-EDTA),then grafted acrylamide(AM)to the LDH-EDTA by a cross-linking method to yield a LDH-EDTA-AM composite;we then evaluated its adsorptive ability for Congo red(CR)and hexavalent chromium(Cr(Ⅵ))in single and binaiy adsorption systems.The adsorption process on LDH-EDTA-AM for CR and Cr(Ⅵ)achieved equilibrium quickly,and the removal efficiencies were minimally affected by initial pH.The maximum uptake quantities of CR and Cr(Ⅵ)on LDH-EDTAAM were 632.9 and 48.47 mg/g,respectively.In mixed systems,chromate removal was stimulated by the presence of CR,while the adsorption efficiency of C R was almost not influenced by coexisting Cr(Ⅵ).The mechanisms involved electrostatic attraction,surface complexation,and anion exchange for the adsorption of both hazardous pollutants.In the Cr(Ⅵ)adsorption process,reduction also took place.The removal efficiencies in real contaminated water were all higher than those in the laboratory solutions. | Jing Li Haiqin Yu Xue Zhang Rixin Zhu Liangguo Yan | 2020 | Frontiers of Environmental Science & Engineering2020，14，3： | 1 |
| 12 | Kinetic Spectmpho-tome~c Determination of Tmoe Manganese ( !] ) with Dahlia Violet inNonionic Micmenmlslon Medium 显示文摘 | Wei Qin Yah Liangguo Chang Guohua et 81 | 2003 | Talanta2003，59，2： | 1 |
| 13 | Talanta显示文摘 | Wei Qin Yan Liangguo Chang Guohua Ou Qingyu | 2003 | 59(2):2532003，59，2： | 1 |
| 14 | Analysis andDesign of a High-Stability, High-Accuracy, Low-Dropout Voltage Regulator 显示文摘 | Shen Liangguo Yan Zushu Wang Zhao | 2007 | Chinese Journal of Semiconduc- tors2007，28，12： | 1 |
| 15 | 显示文摘 | Yan Liangguo Shan Xiaoquan Wen Bei | 2008 | Journal of Hazardous Materials2008，156，13： | 1 |
| 16 | Secreted CREG inhibits cell proliferation mediated by mannose 6‐phosphate/insulin‐like growth factor II receptor in NIH3T3 fibroblasts显示文摘 | Ya‐LingHan PengGuo Ming‐YuSun LiangGuo BoLuan JianKang Cheng‐HuiYan Shao‐HuaLi | 2008 | Genes to Cells2008，，9： | 1 |
| 17 | Effect of lead on the sorption of phenol onto montmorillonites and organo-montmorillonites显示文摘 | Yan Liangguo Xiaoquan Shan Bei Wen | | 0，，01： | 1 |
| 18 | Effect of copper on the adsorption of p -nitrophenol onto soils显示文摘 | Zhi-guo Pei Xiao-quan Shan Bei Wen Shuzhen Zhang Liangguo Yan Shahamat U. Khan | 2005 | Environmental Pollution2005，，3： | 1 |
| 19 | Superiority of the modified T6nnis angle over the T6nnis angle in the radiographic diagnosis of acetabular dysplasia显示文摘 | Liangguo Fa Qing Wang Xiangxing Ma | 2014 | Exp Ther Med2014，8，6： | 1 |
| 20 | Highly effective visible-photocatalytic hydrogen evolution and simultaneous organic pollutant degradation over an urchin-like oxygen-doped MoS_(2)/ZnIn_(2)S_(4)composite显示文摘Achieving hydrogen production and simultaneous decomposition of organic pollutants through dual-functional photocatalytic reactions has received increasing attention due to the environmentally friendly and cost-effective characteristics of this approach.In this work,an urchin-like oxygen-doped MoS_(2)/ZnIn_(2)S_(4)(OMS/ZIS)composite was fabricated for the first time using a simple solvothermal method.The unique microstructure with abundant active sites and fast charge transfer channels further shortened the charge migration distance and compressed carrier recombination.The obtained composite exhibited an efficient H2 evolution reaction rate of 12.8 mmol/g/h under visible light,which was nearly times higher than pristine ZnIn_(2)S_(4),and the apparent quantum efficiency was 14.9%(420 nm).The results of the simultaneous photocatalytic H2 evolution and organic pollutant decomposition test were satisfactory,resulting in decomposition efficiencies of resorcinol,tetracycline,and bisphenol A that reached 41.5%,63.5%,and 53.0%after 4 h,respectively,and the highest H2 evolution rate was 672.7 umol/g/h for bisphenol A.Furthermore,natural organic matter(NOM)abundantly found in actual water was adopted as an electron donor for H production under simulated sunlight irradiation,indicating the promising practicability of simultaneous hydrogen evolution and NOM decomposition.Moreover,the mechanisms of the dual-purpose photocatalytic reactions,as well as the synergistic effect between the molecular structures of the organic pollutants and the corresponding adsorption behavior on the photocatalyst surface were illustrated in detail.These obtained results may serve as an inspiration for the rational design of highly efficient,dual-functional photocatalysts in the future. | Tao Yan Qianqian Yang Rui Feng Xiang Ren Yanxia Zhao Meng Sun Liangguo Yan Qin Wei | 2022 | Frontiers of Environmental Science & Engineering2022，16，10： | 1 |
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