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| 1 | Expression pattern of epithelial cell adhesion molecule on normal and malignant colon tissues显示文摘AIM: To investigate the expression pattern of epithelial cell adhesion molecule (Ep-CAM) on normal and malignant colon tissues to evaluate its diagnostic and therapeutic significance.METHODS: cDNA encoding Ep-CAv extracellular domain was cloned by reverse transcription-polymerase chain reaction (RT-PCR) from excised malignant colon tissues and inserted into a glutathione S-transferase (GST)-tagged vector. EpCAM-GST fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG) and purified with glutathionesepharose. The Ep-CAM-GST fusion protein was mixed with Freund's adjuvant and Balb/c mice were immunized with it. Sp2/0 myeloma cells were fused with the spleen cells of the immunized mice. After having selected by indirect ELISA, the anti-Ep-CAM monoclonal antibodies (NAbs) were generated and the corresponding ascites were obtained.Finally, the human colon carcinoma tissue array prepared from seventy individual patients was stained with the antiEp-CAM NAbs.RESULTS: The isolated Ep-CAM cDNA sequence was identical to the data in GenBank. The expressed fusion protein was almost soluble and had a molecular weight (NW) of 53 ku.Four NAbs against Ep-CAM were obtained and designated as FMU-Ep1, FMU-Ep2, FMU-Ep3 and FMU-Ep4 respectively.Among them, FMU-Ep4 could recognize the natural EpCAM on Colo205 and SW480 cells, and all of them could be used for immunohistochemical staining of tissue sections.It was found that Ep-CAM was distributed differently in normal and various malignant colon tissues, including squamous cell carcinoma, signet-ring cell carcinoma and adenocarcinoma.In normal colon gland epithelia, Ep-CAM antigen was mainly distributed on the basolateral membrane and in the region between the basolateral membrane and the cytoplastic part near the nuclei, whereas the expression pattern of colon malignancies was mainly on the whole surface of epithelia and the expression was much higher than the normal colon tissues. The staining pattern of tissue array showed in adenocarcinoma and papillary adenocarcinoma, and the expression of Ep-CAM was increased from grade Ⅰ to grade Ⅲ.CONCLUSION: NAbs against Ep-CAM might be useful for research on the structure and function of Ep-CAM and may have diagnostic and therapeutic value to various colon carcinomas. | XinXie Chun-YanWang Yun-XinCao WeiWang RanZhuang Li-HuaChen Na-NaDang LiangFang Bo-QuanJin | 2005 | World Journal of Gastroenterology2005，11，3： | 7 |
| 2 | Density of Ni-Al Alloys in Liquid and Solid-Liquid Coexistence State Measured by a Modified Pycnometric Method显示文摘The density of Ni-Al alloys in both liquid state and solid-liquid coexistence state was measured with a modified pycnometric method. It was found that the density of NI-Al alloys decreases with increasing temperature and Al concentration in the alloys. The molar volume of liquid Ni-Al binary alloys increases with the increase of temperature and Al concentration. The partial molar volume of Al in NI-Al binary alloy was calculated approximately. The molar volume of liquid NI-Al alloy determined in the present work shows a negative deviation from the ideal linear molar volume. | LiangFANG FengXIAO ZushuLI ZainanTAO | 2004 | Journal of Materials Science & Technology2004，20，4： | 5 |
| 3 | Density of Liquid Ni-Mo Alloys Measured by a Modified Sessile Drop Method显示文摘The density of liquid binary Ni-Mo alloys with molybdenum concentration from 0 to 20% (mass fraction) was measured by a modified sessile drop method. It has been found that the density of the liquid Ni-Mo alloys decreases with increasing temperature, but increases with the increase of molybdenum concentration in the alloys. The molar volume of liquid Ni-Mo binary alloys increases with the increase of temperature and molybdenum concentration. The partial molar volume of molybdenum in Ni-Mo binary alloy has been approximately calculated as [13.18 - 2.65 × 10-3T + (-47.94 + 3.10 × 10-2T) × 10-2XMo] × 10-6m3·mol-1. The molar volume of Ni-Mo alloy determined in the present work shows a negative deviation from the ideal linear mixing molar volume. | LiangFANG ZushuLI ZaiNanTAO FengXIAO | 2004 | Journal of Materials Science & Technology2004，20，3： | 4 |
| 4 | Density and Structure Analysis of Molten Ni-W Alloys显示文摘Density of molten Ni and Ni-W alloys was measured in the temperature range of 1773~1873 K with a sessile drop method.The density of molten Ni and Ni-W alloys trends to decrease with increasing temperature. The density and molar volume of the alloys trend to increase with increasing W concentration in the alloys. The calculation result shows an ideal mixing of Ni-W alloys. | FengXIAO LiangFANG | 2004 | Journal of Materials Science & Technology2004，20，4： | 3 |
| 5 | Salivary microbiota may predict the presence of esophageal squamous cell carcinoma显示文摘The aim is to explore the predictive value of salivary bacteria for the presence of esophageal squamous cell carcinoma (ESCC). Saliva samples were obtained from 178 patients with ESCC and 101 healthy controls, and allocated to screening and verification cohorts, respectively. In the screening phase, after saliva DNA was extracted, 16S rRNA V4 regions of salivary bacteria were amplified by polymerase chain reaction (PCR) with high-throughput sequencing. Highly expressed target bacteria were screened by Operational Taxonomic Units clustering, species annotation and microbial diversity assessment. In the verification phase, the expression levels of target bacteria identified in the screening phase were verified by absolute quantitative PCR (Q-PCR). Receiver operating characteristic (ROC) curves were plotted to investigate the predictive value of target salivary bacteria. LEfSe analysis revealed higher proportions of Fusobacterium, Streptococcus and Porphyromonas, and Q-PCR assay showed significantly higher numbers of Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in patients with ESCC, when compared with healthy controls (all P < 0.05). The areas under the ROC curves for Streptococcus salivarius, Fusobacterium nucleatum, Porphyromonas gingivalis and the combination of the three bacteria for predicting patients with ESCC were 69%, 56.5%, 61.8% and 76.4%, respectively. The sensitivities corresponding to cutoff value were 69.3%, 22.7%, 35.2% and 86.4%, respectively, and the matched specificity were 78.4%, 96.1%, 90.2% and 58.8%, respectively. These highly expressed Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in the saliva, alone or in combination, indicate their predictive value for ESCC. | Junmin Wei Ruifeng Li Yanxian Lu Fan Meng Bohong Xian Xiaorong Lai Xiayi Lin Yu Deng Dongyang Yang Huabin Zhang Liangfang Li Xiaosong Ben Guibin Qiao Wanwei Liu Zijun Li | 2022 | Genes & Diseases2022，9，4： | 2 |
| 6 | Three-Dimensional Conformal Radiotherapy for Rectal Cancer and the Changes in Cancer Multi-biomarkers显示文摘OBJECTIVE To investigate the clinical efficacy of three-dimensional conformal radiotherapy(3D-CRT)for local y advanced or postoperatively relapsed rectal cancer,and to examine the changes in cancer multi-biomarkers. METHODS Sixty patients with locally advanced or postoperatively relapsed rectal cancer were randomly divided into two groups after 40 Gy external radiation,namely a late-course 3D-CRT group and a conventional radiotherapy group that served as the control.There were 30 patients in each group.For patients in the 3D-CRT group,multi-biomarkers were measured before and after radiotherapy and after relapse. RESULTS Response rates in the 3D-CRT and the control groups were 86.7%(26/30)and 70%(21/30)respectively,without a significant difference (P>0.05).The 1-,2-and 3-year survival rates were 80%,53.3%and 36.7% in the 3D-CRT group;in the control group the rates were 56.7%,40%and 13.3%respectively,with a significant difference(P=0.0213).CEA,CA19-9, CA242 and FER decreased after radiotherapy in the 3D-CRT group,P<0.01, indicating a significant difference.The values after relapse were higher than those without relapse,P<0.01,indicating a significant difference. CONCLUSION Conventional radiotherapy with a 3D-CRT boost gives better therapeutic effect to patients with locally advanced or postoperatively local y relapsed rectal cancer.A multi-biomarker protein chip diagnosis system can be utilized as an effective tool to determine the therapeutic effect and prognosis. | Rui Wei Yangde Zhang Jiantai He Liangfang Shen Jidong Hong Haijun Wu | 2007 | Chinese Journal of Clinical Oncology2007，4，6： | 2 |
| 7 | Engineered Biomimetic Platelet Membrane-Coated Nanoparticles Block Staphylococcus aureus Cytotoxicity and Protect Against Lethal Systemic Infection显示文摘Staphylococcus aureus(S.aureus)is a leading human pathogen capable of producing severe invasive infections such as bacteremia,sepsis,and endocarditis with high morbidity and mortality,exacerbated by the increasingly widespread antibiotic resistance exemplified by methicillin-resistant strains(MRSA).S.aureus pathogenesis is fueled by the secretion of toxins—such as the membrane-damaging pore-forming atoxin,which have diverse cellular targets including the epithelium,endothelium,leukocytes,and platelets.Here,we examine the use of human platelet membrane-coated nanoparticles(PNPs)as a biomimetic decoy strategy to neutralize S.aureus toxins and preserve host cell defense functions.The PNPs blocked platelet damage induced by S.aureus secreted toxins,thereby supporting platelet activation and bactericidal activity.Likewise,the PNPs blocked macrophage damage induced by S.aureus secreted toxins,thus supporting macrophage oxidative burst,nitric oxide production,and bactericidal activity,and diminishing MRSA-induced neutrophil extracellular trap release.In a mouse model of MRSA systemic infection,PNP administration reduced bacterial counts in the blood and protected against mortality.Taken together,the results from the present work provide a proof of principle of the therapeutic benefit of PNPs in toxin neutralization,cytoprotection,and increased host resistance to invasive S.aureus infection. | Jwa-Kyung Kim Satoshi Uchiyama Hua Gong Alexandra Stream Liangfang Zhang Victor Nizet | 2021 | Engineering2021，7，8： | 2 |
| 8 | The direct pyrolysis and catalytic pyrolysis of Nannochloropsis sp. residue for renewable bio-oils显示文摘 | Pan Pan Changwei Hu Wenyan Yang Yuesong Li Linlin Dong Liangfang Zhu Dongmei Tong Renwei Qing Yong Fan | 2010 | Bioresource Technology2010，，： | 1 |
| 9 | Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties显示文摘The dialysis method has been traditionally used for the conversion of native human plasminogen(Glu-Hpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase(SK) and chemical modification (agent(4-amidinophenyl-4′-aminobenzoate hydrochloride)) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquid-phase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits. | TENGLi-rong LIANGFang SUNHong QINYi HONGShui-sheng CHENJia LIULan-ying | 2004 | Chemical Research in Chinese Universities2004，20，5： | 1 |
| 10 | PLGA–lecithin–PEG core–shell nanoparticles for controlled drug delivery显示文摘 | Juliana M. Chan Liangfang Zhang Kai P. Yuet Grace Liao June-Wha Rhee Robert Langer Omid C. Farokhzad | 2008 | Biomaterials2008，，8： | 1 |
| 11 | Nature of vanadium species on vanadium silicalite-1 zeolite and their stabil ity in hydroxylation reaction of benzene to phenol显示文摘 | GUO Bin ZHU Liangfang HU Xiaoke | 2011 | Catalysis Science & Technology2011，1，6： | 1 |
| 12 | KLF17 empowers TGF- 显示文摘 | Ali A Zhang P Liangfang Y | 2015 | Cell Death Dis2015，6，： | 1 |
| 13 | Surface Tension of Molten Ni and Ni-Co Alloys显示文摘Surface tension of molten Ni and Ni-Co (5 and 10 mass fraction) alloys was measured at the temperature range of 1773~1873 K using an improved sessile drop method with an alumina substrate in an Ar+3%H2 atmosphere. The error of the data obtained was analyzed. The surface tension of molten Ni and Ni-Co (5 and 10 mass fraction) alloys decreases with increasing temperature. The influence of Co on the surface tension of Ni-Co alloys is little in the studied Co concentration range. | FengXIAO LiangFANG KiyoshiNOGI | 2005 | Journal of Materials Science & Technology2005，21，2： | 1 |
| 14 | Single‐dose, placebo‐controlled, randomized study of AMG 785, a sclerostin monoclonal antibody显示文摘 | DesmondPadhi GrahamJang BrianStouch LiangFang EdwardPosvar | 2010 | J Bone Miner Res2010，，1： | 1 |
| 15 | Management of a pa- tient with faciocervical burns and inhalational injury due to hydrofluoric acid exposure 显示文摘 | Yuanbai Z Xingang W Liangfang N | 2014 | Int J Low Extrem Wounds2014，13，2： | 1 |
| 16 | PLGA–lecithin–PEG core–shell nanoparticles for controlled drug delivery显示文摘 | Juliana M. Chan Liangfang Zhang Kai P. Yuet Grace Liao June-Wha Rhee Robert Langer Omid C. Farokhzad | 2008 | Biomaterials2008，，8： | 1 |
| 17 | Two-step hydrothermal conversion of Pubescens to obtain furans and phenol compounds separately显示文摘 | Jia Luo Yong Xu Lingjie Zhao Linlin Dong Dongmei Tong Liangfang Zhu Changwei Hu | 2010 | Bioresource Technology2010，，22： | 1 |
| 18 | Preparation and characterization of waterborne epoxy latexes and epoxy/(silica sol) composites显示文摘 | Qian Zhao Rong Guan Liangfang Zhai Qing Ye Niannian Fan | 2014 | J Vinyl Addit Technol2014，，1： | 1 |
| 19 | Nanoparticle-based combination therapy toward overcoming drug resistance in cancer显示文摘 | Che-Ming Jack Hu Liangfang Zhang | 2012 | Biochemical Pharmacology2012，，8： | 1 |
| 20 | The transcription factor KLF14 regulates macrophage glycolysis and immune function by inhibiting HK2 in sepsis显示文摘Sepsis is a heterogeneous syndrome induced by a dysregulated host response to infection.Glycolysis plays a role in maintaining the immune function of macrophages,which is crucial for severely septic patients.However,how the pathways that link glycolysis and macrophages are regulated is still largely unknown.Here,we provide evidence to support the function of KLF14,a novel Krüppel-like transcription factor,in the regulation of glycolysis and the immune function of macrophages during sepsis.KLF14 deletion led to significantly increased mortality in lethal models of murine endotoxemia and sepsis.Mechanistically,KLF14 decreased glycolysis and the secretion of inflammatory cytokines by macrophages by inhibiting the transcription of HK2.In addition,we confirmed that the expression of KLF14 was upregulated in septic patients.Furthermore,pharmacological activation of KLF14 conferred protection against sepsis in mice.These findings uncover a key role of KLF14 in modulating the inflammatory signaling pathway and shed light on the development of KLF14-targeted therapeutics for sepsis. | Yuan Yuan Guangjian Fan Yuqi Liu Lu Liu Tong Zhang Pengfei Liu Qing Tu Xinyi Zhang Shiyuan Luo Liangfang Yao Feng Chen Jingbao Li | 2022 | Cellular & Molecular Immunology2022，19，4： | 1 |
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