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| 1 | Artemisinin mimics calorie restriction to extend yeast lifespan via a dual-phase mode: a conclusion drawn from global transcriptome profiling显示文摘Calorie restriction(CR) promotes longevity among distinct organisms from yeast to mammals. Although CR-prolonged lifespan is believed to associate with enhanced respiratory activity, it is apparently controversial for accelerated energy consumption regardless of insufficient nutrient intake. In reconciling the contradiction of less food supply versus much metabolite dispense, we revealed a CR-based mode of dual-phase responses that encompass a phase of mitochondrial enhancement(ME) and a phase of post-mitochondrial enhancement(PME), which can be distinguished by the expression patterns and activity dynamics of mitochondrial signatures. ME is characterized by global antioxidative activation, and PME is denoted by systemic metabolic modulation. CR-mediated aging-delaying effects are replicated by artesunate, a semi-synthetic derivative of the antimalarial artemisinin that can alkylate heme-containing proteins, suggesting artesunate-heme conjugation functionally resembles nitric oxide-heme interaction. A correlation of artesunate-heme conjugation with cytochrome c oxidase activation has been established from adduct formation and activity alteration. Exogenous hydrogen peroxide also mimics CR to trigger antioxidant responses, affect signaling cascades, and alter respiratory rhythms, implying hydrogen peroxide is engaged in lifespan extension. Conclusively, artesunate mimics CR-triggered nitric oxide and hydrogen peroxide to induce antioxidative networks for scavenging reactive oxygen species and mitigating oxidative stress, thereby directing metabolic conversion from anabolism to catabolism, maintaining essential metabolic functionality, and extending life expectancy in yeast. | WANG DaTing WU Ming LI SiMing GAO Qian ZENG QingPing | 2015 | Science China(Life Sciences)2015，58，5： | 6 |
| 2 | Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone 显示文摘 | Zhao ZJ Date T Li YH | 2005 | J Gen Virol2005，86，8： | 1 |
| 3 | Characterization of the E 138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone 显示文摘 | Zhao Z Date T Li Y | 2005 | J GenVirol2005，86，8： | 1 |
| 4 | Analysis of floating buoy of a wave jack-up platform Haiyuan 1 显示文摘 | Li Date Li Detang Li Fei | 2013 | Advances in Mechanical Engineering2013，10，507： | 1 |
| 5 | Changes in breast cancer incidence rates in theUnited States by histologic subtype and race/ethnicity, 1995 to2004显示文摘 | Li Cl Dating JR | 2007 | Cancer Epidemiol Biomarkers Prev2007，16，12： | 1 |
| 6 | Characterization of the E-138(Glu/ Lys)mutation in Japanese encephalitis virus by using a stable,full-length,infectious cDNA clone显示文摘 | Zhao Z Date T Li Y | 2005 | J Gen Virol2005，86，8： | 1 |
| 7 | Characterization of the E-137, (Glu/Lys) mtltation in Japanese encephalitis virus by using a stable, lull-length, infectious eDNA clone 显示文摘 | Zhao ZJ Date T Li YH | 2005 | J Gen Virol2005，86，8： | 1 |
| 8 | Characterization of the E-138 ( Glu/ Lys) mutation in Japanese encephalitis virus by using a stable, full-length,infectious cDNA clone 显示文摘 | Zhao Z Date T Li Y | 2005 | J Gen Virol2005，86，8： | 1 |
| 9 | Characterization of the E-138(Glu/Lys)mutation in Japanese encephalitis virus by using a stable,fulllength,infectious cDNA clone显示文摘 | Zhao ZJ Date T Li Y | | 0，，08： | 1 |
| 10 | Hadoop framework: impact of data organization on performance显示文摘 | Yu Shyang Tan Jiaqi Tan Eng Siong Chng Bu‐Sung Lee Jiaming Li Susumu Date Hui Ping Chak Xiong Xiao Atsushi Narishige | 2013 | Exper2013，，11： | 1 |
| 11 | Positivity-preserving H∞ model reduction for positive systems显示文摘 | LI P LAM J WANG Z DATE P | 2011 | Automatica2011，47，7： | 1 |
| 12 | High-throughput genome editing in rice with a virus-based surrogate system显示文摘With the widespread use of clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas) technologies in plants, large-scale genome editing is increasingly needed. Here, we developed a geminivirus-mediated surrogate system, called Wheat Dwarf Virus-Gate(WDV-surrogate), to facilitate high-throughput genome editing.WDV-Gate has two parts: one is the recipient callus from a transgenic rice line expressing Cas9 and a mutated hygromycin-resistant gene(HygM) for surrogate selection;the other is a WDV-based construct expressing two single guide RNAs(sgRNAs) targeting HygM and a gene of interest, respectively. We evaluated WDV-Gate on six rice loci by producing a total of 874 T_0 plants. Compared with the conventional method, the WDV-Gate system, which was characterized by a transient and high level of sgRNA expression, significantly increased editing frequency(66.8% vs. 90.1%), plantlet regeneration efficiency(2.31-fold increase), and numbers of homozygous-edited plants(36.3%vs. 70.7%). Large-scale editing using pooled sg RNAs targeting the SLR1 gene resulted in a high editing frequency of 94.4%, further demonstrating its feasibility. We also tested WDVGate on sequence knock-in for protein tagging.By co-delivering a chemically modified donor DNA with the WDV-Gate plasmid, 3xFLAG peptides were successfully fused to three loci with an efficiency of up to 13%. Thus, by combining transiently expressed sgRNAs and a surrogate selection system, WDV-Gate could be useful for high-throughput gene knock-out and sequence knock-in. | Yifu Tian Dating Zhong Xinbo Li Rundong Shen Han Han Yuqin Dai Qi Yao Xuening Zhang Qi Deng Xuesong Cao Jian-Kang Zhu Yuming Lu | 2023 | Journal of Integrative Plant Biology2023，65，3： | 0 |
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