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| 1 | cytokine 的角色像受体的因素 1 在肝的星形的房间和纤维变性显示文摘 AIM:To elucidate the role of cytokine receptor-like factor 1(CRLF1) in hepatic stellate cells and liver fibrosis.METHODS:Rat hepatic stellate cells(HSCs) were isolated by Nykodenz gradient centrifugation and activated by culturing in vitro.Differentially expressed genes in quiescent and culture activated HSCs were identified using microarrays.Injections of carbon tetrachloride(CCl 4) for 4 wk were employed to induce liver fibrosis.The degree of fibrosis was assessed by Sirius red staining.Adenovirus expressing CRLF1 was injected through tail vein into mice to achieve overexpression of CRLF1 in the liver.The same adenovirus was used to overexpress CRLF1 in quiescent HSCs cultured in vitro.Expression of CRLF1,CLCF1 and ciliary neurotrophic factor receptor(CNTFR) in hepatic stellate cells and fibrotic livers was analyzed by semi-quantitative reverse transcription-polymerase chain reaction and Western blotting.Expression of profibrotic cytokines and collagens was analyzed by the same method.RESULTS:CRLF1 is a secreted cytokine with unknown function.Human mutations suggested a role in development of autonomous nervous system and a role of CRLF1 in immune response was implied by its similarity to interleukin(IL)-6.Here we show that expression of CRLF1 was undetectable in quiescent HSCs and was highly upregulated in activated HSCs.Likewise,expression of CRLF1 was very low in normal livers,but was highly upregulated in fibrotic livers,where its expression correlated with the degree of fibrosis.A cofactor of CLRF1,cardiotrophin-like cytokine factor 1(CLCF1),and the receptor which binds CRLF1/CLCF1 dimer,the CNTFR,were expressed to similar levels in quiescent and activated HSCs and in normal and fibrotic livers,indicating a constitutive expression.Overexpression of CLRF1 alone in the normal liver did not stimulate expression of profibrotic cytokines,suggesting that the factor itself is not pro-inflammatory.Ectopic expression in quiescent HSCs,however,retarded their activation into myofibroblasts and specifically decreased expression of type Ⅲ collagen.Inhibition of type Ⅲ collagen expression by CRLF1 was also seen in the whole liver.Our results suggest that CLRF1 is the only component of the CRLF1/CLCF1/CNTFR signaling system that is inducible by a profibrotic stimulus and that activation of this system by CLRF1 may regulate expression of type Ⅲ collagen in fibrosis.CONCLUSION:By regulating activation of HSCs and expression of type Ⅲ collagen,CRLF1 may have an ability to change the composition of extracellular matrix in fibrosis. | Lela Stefanovic Branko Stefanovic | 2012 | World Journal of Hepatology2012，4，12： | 3 |
| 2 | IL28B favorable genotype and ultrarapid viral response as the earliest treatment predictors of a sustained viral response in a Georgian cohort infected with the hepatitis C genotype 1显示文摘 | Marine Karchava Lali Sharvadze Nikoloz Chkhartishvili Kenrad Nelson Nino Gochitashivli Lana Gatserelia Natia Dvali Ekaterine Dolmazashvili Lela Dzigua Nino Badridze Maia Zhamutashvili Tengiz Tsertsvadze | 2012 | European Journal of Gastroenterology & Hepatology2012，，7： | 1 |
| 3 | Echocardiographic assessment of left ventricular hypertrophy:comparison to necropsy finding显示文摘 | Devereux RB Alonso DR Lelas EM | 1986 | Am J Cardiol1986，57，： | 1 |
| 4 | Detecting Food and Waterborne Viruses by Surface - Enhanced Raman Spectroscopy显示文摘 | Cui Fan Zhiqiang I-Iu Lela K Riley | 2010 | Journal of Food Science2010，75，5： | 1 |
| 5 | Physical properties of ultrasound treated soy proteins显示文摘 | Jambrak A R Lelas V Mason T J | 2009 | Journal of Food Engineering2009，93，： | 1 |
| 6 | Physical properties of ultrasound treated soy proteins显示文摘 | Jambrak A R Lelas V Mason T | | 0，，04： | 1 |
| 7 | Physical properties ofultrasound treated soy proteins显示文摘 | JAMBRAK A R LELAS V MASON T J | 2009 | Journal of Food Engineering2009，93，4： | 1 |
| 8 | Effect of ultrasound treatment on solubility and foaming properties of whey protein suspensions 显示文摘 | Jambrak A R Mason T J Lelas V | 2008 | Journal of Food Engineering2008，86，： | 1 |
| 9 | Decreasing of oil absorption in potato strips during deep fat frying显示文摘 | Rimac-Brncic S Lelas V Rade D | 2004 | Journal of Food Engineering2004，64，2： | 1 |
| 10 | Physical properties of ultrasound treated soy proteins 显示文摘 | Jambrak A R Lelas V Mason T J | 2009 | Journal of Food Engineering2009，93，： | 1 |
| 11 | Development of complete heart block in an adult patient with Sjoegren's Syndrome and anti-Ro/SS-A autoantibodies显示文摘 | Lela A Lee Michael B Pickell MR et at | 1996 | Arthritis Rheumatism1996，39，8： | 1 |
| 12 | Development of complete heart block in an adult patient with Sjogren's syndrome and anti-Ro/SS-A autoantibodies显示文摘 | Lela A Lee Michael B Pickell | 1996 | Arthritis Rheumatism1996，39，8： | 1 |
| 13 | The anxiolytic CRF1 an-tagonist DMP696 fails to function as a discriminative stimulus anddoes not substitute for chlordiazepoxide in rats显示文摘 | Lelas S Zeller KM Ward KA | 2003 | Psychophar-macology2003，166，4： | 1 |
| 14 | Effect of ultrasound treatment on particle size and molecular weight ofwhey proteins显示文摘 | Jambrak Ar Mason Tj Lelas V | 2014 | Journal of Food Engineering2014，121，： | 1 |
| 15 | Effects of high pressure on functionality of whey protein concentrate and whey protein isolate 显示文摘 | Kreaid G Lelas V Heceg Z | 2006 | Lait2006，86，： | 1 |
| 16 | Identification of routine Helicobacter by PCR and restriction enzyme analyses显示文摘 | Lela K Riley Craig L | 1996 | J Clin Microbiol1996，34，4： | 1 |
| 17 | Physical proper- ties of ultrasound treated soy proteins显示文摘 | JAMBRAK A R LELAS V MASON T J | 2009 | Journal of Food En-gineering2009，93，4： | 1 |
| 18 | Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends 显示文摘 | Claus M Azzalin Patrick Reichenbach Lela Khoriauli | 2007 | Science2007，318，5851： | 1 |
| 19 | Comparison of Conventional and Ultrasound Assisted Extraction Techniques of Yellow Tea and Bioactive Composition of Obtained Extracts显示文摘 | Dunja Hor?i? Anet Re?ek Jambrak Ana Bel??ak-Cvitanovi? Dra?enka Komes Vesna Lelas | 2012 | Food and Bioprocess Technology2012，，： | 1 |
| 20 | Ultrasonic effect on physicochemical and functional properties of cL-lactalbumin 显示文摘 | Jambrak A R Mason T J Lelas V | 2010 | LWT Food Science Technology2010，43，2： | 1 |
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