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| 1 | Initial Combination Therapy Reduces the Risk of Cardiovascular Events in Hypertensive Patients: A Matched Cohort Study显示文摘 | Alan H. Gradman Hélène Parisé Patrick Lefebvre Heather Falvey Marie-Hélène Lafeuille Mei Sheng Duh | 2013 | Hypertension2013，，2： | 2 |
| 2 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 3 | Parallel ex- pression of SOX9 and Col2a1 in cells undergoing ehon- drogenesis显示文摘 | Zhao Q Eberspaeeher H Lefebvre V | 1997 | Dev Dyn1997，209，4： | 1 |
| 4 | Spray characteristics of aerated liquid pressure atomizers 显示文摘 | Lefebvre A H Wang X F Martin C A | 1988 | AIAA J Prop Power1988，4，4： | 1 |
| 5 | Parallel expression of Sox9 and Col2a1 in cells undergoing chondrogenesis显示文摘 | Zhao Q Eberspaecher H Lefebvre V | 1997 | Dev Dyn1997，209，： | 1 |
| 6 | Weak Extinction Limits of Turbulence Heterogeneous Fuel/Air Mixtures显示文摘 | Lefebvre A H | 1980 | Transactions of ASME Journal of Engineering for Power1980，102，： | 1 |
| 7 | Steroid perfusion of the inner ear for sudden sensorineural hearing loss after failure of conventional therapy:a pilot study显示文摘 | LEFEBVRE P P STAECKER H | 2002 | Acta Otolaryngol2002，122，： | 1 |
| 8 | Precipitation kinetic of Al3(Sc,Zr) dispersoids in aluminium 显示文摘 | Lefebvre W Danoix F Hallem H | 2009 | Journal of Alloys and Compounds2009，470，12： | 1 |
| 9 | A novel method of atomization with potentialgas turbine application显示文摘 | Lefebvre A H | 1988 | Indian Defence Sci1988，38，： | 1 |
| 10 | Steroid perfusion of the inner ear for sudden sensorineural hearing loss after failure of conventional therapy:a pilot study显示文摘 | Lefebvre PP Stacker H | 2002 | Acta Otolaryngol2002，122，： | 1 |
| 11 | A design procedure for effervescent atomizers显示文摘 | Chin J S Lefebvre A H | 1995 | Journal of Engineering for Gas Turbines andPower1995，117，2： | 1 |
| 12 | Preliminary experimental investigation of the pressure evolution in detonation cells显示文摘 | Hanana M Lefebvre M H Tiggelen P J V | 2000 | Experimental Thermal and Fluid Science2000，21，： | 1 |
| 13 | Parallel controllable texture synthesis 显示文摘 | Lefebvre S Hoppe H | 2005 | ACM Transactions on Graphics2005，24，3： | 1 |
| 14 | Influence of flow conditions on deposits fron~ heated hydrocarbon fuels 显示文摘 | / Chin J S Lefebvre A H | 1993 | Journal of Engineering for Gas Turbines] / and Power1993，115，： | 1 |
| 15 | Effects of incorrect vene-puncture on plasma creatine kinase activity in dog and horse显示文摘 | Fayolle P Lefebvre H Braun J P | 1992 | Br Vet J1992，148，： | 1 |
| 16 | Steroid perfusion of the inner ear for sudden sebsorineual hearing loss after failure ofconventional therapy:a pilot study 显示文摘 | Lefebvre PP Staecker H | 2002 | Acta Otolaryngol2002，122，7： | 1 |
| 17 | Evaluation of the treatment of TSH-secreting pitutary adenomas with a slow release formulation of the somatostatin analog lanreotide显示文摘 | Kuhn JM Arlot S Lefebvre H | 2000 | J Clin Endocrinol Metab2000，85，4： | 1 |
| 18 | Surface organometallic chemistry of Sn: grafting reaction on HY zeolite supercage显示文摘 | Zhao H Lefebvre F | 2000 | Chem Letters2000，10，： | 1 |
| 19 | Parallel expression of Sox9 and Col2al in cells undergoing chondrogenesis 显示文摘 | ZHAO Q EBERsPAEcHER H LEFEBVRE V | 1997 | Dev Dyn1997，209，4： | 1 |
| 20 | Transient and har- monic eddy currents: lift-off point of intersection 显示文摘 | Mandache C Lefebvre J H V | 2006 | NDT&E International2006，39，7： | 1 |
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