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| 1 | A-DA′D-A non-fullerene acceptors for high-performance organic solar cells显示文摘Since the world-record power conversion efficiency of 15.7%was achieved for organic solar cells(OSCs)in 2019,the newly developed non-fullerene acceptor(NFA)Y6 with an A-DA′D-A structure(A denotes an electron-accepting moiety,D denotes an electron-donating moiety)has attracted increasing attention.Subsequently,many new A-DA′D-A NFAs have been designed and synthesized,and the A-DA′D-A NFAs have played a significant role in the development of high-performance non-fullerene organic solar cells(NF-OSCs).Compared with the classical A-D-A-type acceptors,A-DA′D-A NFAs contain an electrondeficient core(such as benzothiadiazole(BT),benzotriazole(BTA),quinoxaline(Qx),or their derivatives)in the ladder-type fused rings to fine-tune the energy levels,broaden light absorption and achieve higher electron mobility of the NFAs.This review emphasizes the recent progress on these emerging A-DA′D-A(including Y-series)NFAs.The synthetic methods of DA′D-fused rings are introduced.The relationships between the chemical structure of the A-DA′D-A NFAs and the photovoltaic performance of the corresponding OSCs are summarized and discussed.Finally,issues and prospects for further improving photovoltaic performance of the OSCs are also proposed. | Qingya Wei Wei Liu Mario Leclerc Jun Yuan Honggang Chen Yingping Zou | 2020 | Science China Chemistry2020，63，10： | 7 |
| 2 | 应用ELISPOT试验测定重组卡介苗诱导的T细胞应答显示文摘应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。 | 焦新安 RichardLo-man EdithD’eriaud Brigitte Gicquel Nathalie Winter Claude Leclerc 刘秀梵 | 2001 | 中国预防兽医学报2001，23，1： | 7 |
| 3 | Mucosal healing progression after acute colitis in mice显示文摘BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required. These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins. However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.METHODS C57 BL/6 male mice were given 3.5% dextran sodium sulfate(DSS) in drinking water for 5 d. They were euthanized 2(day 7), 5(day 10), 8(day 13), and 23(day28) d after DSS removal. The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0. Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16 S rRNA sequencing.RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa. It was recorded 2 d after termination of the DSStreatment, followed by a progressive return to values similar to those of control mice. Although signs of colitis were severe(inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations(hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of certain key epithelial repair modulators, including transforming growth factor-β, interleukin(Il)-15, Il-22, Il-33, and serum amyloid A. Whereas signs of inflammation progressively diminished, luminal colonic environment alterations and microscopic abnormalities of colon mucosa persisted long after colitis induction.CONCLUSION This study shows that colon repair can be initiated in the context of inflamed mucosa associated with alterations of the luminal environment and highlights the longitudinal involvement of key modulators. | Sandra Vidal-Lletjós Mireille Andriamihaja Anne Blais Marta Grauso Patricia Lepage Anne-Marie Davila Claire Gaudichon Marion Leclerc Francois Blachier Annaig Lan | 2019 | World Journal of Gastroenterology2019，25，27： | 5 |
| 4 | 融合表达淋巴细胞脉络丛脑膜炎病毒和卵清白蛋白CD8^+T细胞表位的减毒鼠伤寒沙门氏菌的构建与鉴定显示文摘依据淋巴细胞脉络丛脑膜炎病毒(LCMV)主要保护性抗原CD8+T细胞表位VRRPQASGVYMGNLTAQ和卵清白蛋白(OVA)CD8+T细胞表位SIINFEKL,设计、合成两条编码LCMV和OVACD8+T细胞表位的寡核苷酸片段,经退火后,克隆入绿色荧光蛋白表达质粒pYAGFP,经PCR扩增和序列测定分析,证实成功构建T细胞表位与绿色荧光蛋白融合表达的重组质粒pYAGFPL_O,将此重组质粒pYAGFPL_O转化减毒鼠伤寒沙门氏菌X4550,获得重组沙门氏菌X4550(pYAGF PL_O)。用SDS_PAGE电泳测得重组菌表达的融合蛋白约为30kD。同时,荧光显微镜下观察到X4550(pYAGFPL_O)发出黄绿色荧光。这些结果表明LCMV和OVAT细胞表位已成功表达。重组菌X4550(pYAGFPL_O)的获得为研究沙门氏菌载体携带外源抗原的T细胞应答规律及调控机理打下了重要基础。 | 王芳 焦新安 潘志明 张晓明 黄金林 Richard Lo-Man Claude Leclerc 刘秀梵 | 2004 | 中国预防兽医学报2004，26，1： | 4 |
| 5 | Identification of Ca2+ signaling components in neural stem/progenitor cells during differentiation into neurons and glia in intact and dissociated zebrafish neurospheres显示文摘The development of the CNS in vertebrate embryos involves the generation of different sub-types of neurons and glia in a complex but highly-ordered spatio-temporal manner. Zebrafish are commonly used for exploring the development, plasticity and regeneration of the CNS, and the recent development of reliable protocols for isolating and culturing neural stem/progenitor cells(NSCs/NPCs) from the brain of adult fish now enables the exploration of mechanisms underlying the induction/specification/differentiation of these cells. Here, we refined a protocol to generate proliferating and differentiating neurospheres from the entire brain of adult zebrafish. We demonstrated via RT-qPCR that some isoforms of ip3 r, ryr and stim are upregulated/downregulated significantly in differentiating neurospheres, and via immunolabelling that 1,4,5-inositol trisphosphate receptor(IP3 R) type-1 and ryanodine receptor(RyR) type-2 are differentially expressed in cells with neuron-or radial glial-like properties. Furthermore, ATP but not caffeine(IP3 R and RyR agonists, respectively), induced the generation of Ca^(2+) transients in cells exhibiting neuron-or glial-like morphology. These results indicate the differential expression of components of the Ca^(2+) -signaling toolkit in proliferating and differentiating cells. Thus, given the complexity of the intact vertebrate brain, neurospheres might be a useful system for exploring neurodegenerative disease diagnosis protocols and drug development using Ca^(2+) signaling as a read-out. | Man Kit Tse Ting Shing Hung Ching Man Chan Tiffany Wong Mike Dorothea Catherine Leclerc Marc Moreau Andrew L.Miller Sarah E.Webb | 2018 | Science China(Life Sciences)2018，61，11： | 4 |
| 6 | 重组减毒细菌运送CD8^+T细胞表位的效应分析显示文摘目的:分析重组减毒菌体外运送CD8+T细胞表位的效应。方法:以表达卵清蛋白(OVA)和淋巴细胞脉络丛脑膜炎病毒(LCMV)CD8+T细胞表位的重组菌13A(ptG2F)、25A(ptG2F)和SL7207(ptG2F)感染抗原提呈细胞(APC)LKb、LLd和骨髓源树突状细胞(BMDC),应用体外抗原提呈试验检测APC对重组菌运送的CD8+T细胞表位的提呈效应。结果:感染试验证实,减毒菌13A、25A和SL7207对LKb细胞或LLd细胞均具有良好的侵袭能力,BMDC对重组菌具有很好的摄取功能。抗原提呈试验结果显示,在感染的早期(2h),LKb、LLd细胞和BMDC均可提呈重组菌13A(ptG2F)或SL7207(ptG2F)运送的T细胞表位;在感染的晚期(48h),LKb细胞对OVA257-264CD8+T细胞表位的提呈效应降低,LLd细胞对LCMV118-132CD8+T细胞表位的提呈效应增强。3种APC均不能提呈25A(ptG2F)运送的T细胞表位。另外,在同样的作用条件下,BMDC对减毒菌运送的抗原表位的提呈效应要强于LKb和LLd细胞。结论:重组菌能运送CD8+T细胞表位,为基于减毒细菌的新型基因工程疫苗的分子设计提供了有益借鉴。 | 潘志明 张晓明 焦新安 Richard Lo-Man Claude Leclerc 刘秀梵 | 2006 | 细胞与分子免疫学杂志2006，22，6： | 4 |
| 7 | TRPC3 is required for the survival, pluripotency and neural differentiation of mouse embryonic stem cells(mESCs)显示文摘Transient receptor potential canonical subfamily member 3(TRPC3) is known to be important for neural development and the formation of neuronal networks. Here, we investigated the role of TRPC3 in undifferentiated mouse embryonic stem cells(mESCs) and during the differentiation of mESCs into neurons. CRISPR/Cas9-mediated knockout(KO) of TRPC3 induced apoptosis and the disruption of mitochondrial membrane potential both in undifferentiated mESCs and in those undergoing neural differentiation. In addition, TRPC3 KO impaired the pluripotency of mESCs. TRPC3 KO also dramatically repressed the neural differentiation of mESCs by inhibiting the expression of markers for neural progenitors, neurons, astrocytes and oligodendrocytes.Taken together, our new data demonstrate an important function of TRPC3 with regards to the survival, pluripotency and neural differentiation of mESCs. | Helen Baixia Hao Sarah E. Webb Jianbo Yue Marc Moreau Catherine Leclerc Andrew L. Miller | 2018 | Science China(Life Sciences)2018，61，3： | 3 |
| 8 | 重组卡介苗表达的MalE蛋白T细胞表位的鉴定显示文摘目的 鉴定重组卡介苗表达的MalE蛋白T细胞表位。方法 用抗原提呈试验、T细胞增殖试验、ELISPOT试验和表位作图测试重组MalE蛋白的T细胞表位。结果 重组BCG MalE功能性表达了MalE蛋白的 4种H 2 d 限制性T细胞表位。结论 在 4种表位中 ,p6 8~ 82是重组MalE蛋白的主要T细胞表位 ,而其余 | 焦新安 Richard Lo-man Edith Driaud Sophie Burgaud Brigitte Gicquel Nathalie Winter Claude Leclerc 刘秀梵 | 2002 | 中华微生物学和免疫学杂志2002，22，1： | 3 |
| 9 | 日粮中添加过瘤胃保护B族维生素对奶牛健康、产奶量和繁殖性能的影响显示文摘围产期日粮中添加过瘤胃保护B族维生素对奶牛健康、产奶量和繁殖性能都会产生影响。试验结果表明,添加过瘤胃保护B族维生素可以显著降低刚分娩奶牛亚临床酮病的发病率、奶牛平均人工授精成本以及泌乳190天内的淘汰率(P<0.05);显著增加乳脂率和泌乳150天怀孕率(P<0.05);胎衣不下的发病率从18.5%减少到16.0%,但是差异不显著。这说明在围产期日粮中添加过瘤胃保护B族维生素可以提高牛奶乳脂率、奶牛健康状况以及繁殖性能,并能为奶牛场节省成本,提高经济效益。 | Essi Evan Helene Leclerc Stacy Wrinkle 詹小立 阳成波 | 2015 | 中国乳业2015，，8： | 2 |
| 10 | Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34^(cdc2) kinase显示文摘Objective: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry.Results: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimonapilosa; Herba solani lyrati; Galla chinesis. Conclusion: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery. | 杨骅 郑树 MEIJER Laurent 李士敏 LECLERC Sophie 郁琳琳 程金泉 张苏展 | 2005 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2005，6，7： | 2 |
| 11 | 重组卡介苗感染树突细胞的研究显示文摘在体内外试验中 ,树突细胞 (DC)均能摄取表达大肠杆菌麦芽糖结合蛋白 (MalE)的重组卡介苗 (rBCG·MalE)。应用流式细胞术测得荧光标记的rBCG·MalE在体内感染DC的水平在 1 % 2 % ,与感染巨噬细胞 (MΦ)的水平相当。体外抗原提呈试验结果显示 ,DC和MΦ能吞噬、加工rBCG·MalE并向T细胞杂交瘤FBU·B1 1提呈MalE蛋白抗原表位 ,而在体内试验中 ,从rBCG·MalE免疫小鼠脾脏中纯化的DC ,可检测到MalE表位MHCⅡ复合物 ,而纯化的MΦs却测不到。这表明DC在起始抗分枝杆菌的免疫保护中发挥关键作用。 | 焦新安 Richard Lo-Man Pierre Guermonprez Edith Dériaud Brigitte Gicquel Nathalie Winter Claude Leclerc 刘秀梵 | 2002 | 扬州大学学报(农业与生命科学版)2002，23，1： | 2 |
| 12 | Modeling and implementation of tandem polymer solar cells using wide-bandgap front cells显示文摘Tandem device architectures offer a route to greatly increase the maximum possible power conversion efficiencies(PCEs)of polymer solar cells,however,the complexity of tandem cell device fabrication(such as selecting bandgaps of the front and back cells,current matching,thickness,and recombination layer optimization)often result in lower PCEs than are observed in single-junction devices.In this study,we analyze the influence of front cell and back cell bandgaps and use transfer matrix modeling to rationally design and optimize effective tandem solar cell structures before actual device fabrication.Our approach allows us to estimate tandem device parameters based on known absorption coefficients and open-circuit voltages of different active layer materials and design devices without wasting valuable time and materials.Using this approach,we have investigated a series of wide bandgap,high voltage photovoltaic polymers as front cells in tandem devices with PTB7-Th as a back cell.In this way,we have been able to demonstrate tandem devices with PCE of up to 12.8%with minimal consumption of valuable photoactive materials in tandem device optimization.This value represents one of the highest PCE values to date for fullerene-based tandem solar cells. | Seo-Jin Ko Hyosung Choi Quoc Viet Hoang Chang Eun Song Pierre-Olivier Morin Jungwoo Heo Mario Leclerc Sung Cheol Yoon Han Young Woo Won Suk Shin Bright Walker Jin Young Kim | 2020 | Carbon Energy2020，2，1： | 2 |
| 13 | Metformin inhibits hepatic gluconeogenesis in mice independently of the LKB1/AMPK pathway via a decrease in hepatic energy state显示文摘 | Foretz Marc Hébrard Sophie Leclerc Jocelyne Zarrinpashneh Elham Soty Maud Mithieux Gilles Sakamoto Kei Andreelli Fabrizio Viollet Benoit | 2010 | Journal of Clinical Investigation2010，，7： | 2 |
| 14 | New approach in vaccine development 显示文摘 | Leclerc C | 2003 | Immunol Microbiol Infect Dis2003，26，： | 1 |
| 15 | Cloning and mRNA expression of the Ca2+-binding DREAM protein in the pituitary显示文摘 | Leclerc G M Leclerc G J Shorte S L | 2002 | Gen Comp Endocrinol2002，129，1： | 1 |
| 16 | The median procedure for partitions显示文摘 | BARTHELEMY J LECLERC B | 2008 | DIMACS Series in Discrete Mathematics2008，41，10： | 1 |
| 17 | Ascorbic acid decreases the binding affinity of the AT1 receptor for angiotensin Ⅱ 显示文摘 | Leclerc PC Proulx CD Arguin G | 2008 | Am J Hypert2008，21，1： | 1 |
| 18 | Phylogeny of dermatophytes and dimorphilic fungi based on large subunit ribosomal RNA sequence comparisons显示文摘 | Leclerc MC Philippe H Gueho E | 1994 | J Med Vet Mycol1994，32，： | 1 |
| 19 | Metformin inhibits hepatic gluconeogenesis in mice independently of the LKB1/AMPK pathway via a decrease in hepatic energy state 显示文摘 | Foretz M H6brard S Leclerc J | 2010 | J Clin Invest2010，120，： | 1 |
| 20 | Clogging of fibrous filters by solid aerosol particles Experimental and modelling study显示文摘 | D. Thomas P. Penicot P. Contal D. Leclerc J. Vendel | 2001 | Chemical Engineering Science2001，，11： | 1 |
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