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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | 活性氧化铝除氟研究显示文摘针对目前活性氧化铝饮水除氟中存在的问题,进行了生产性试验研究,通过采用原水投加CO_2气体降低pH值、小颗粒活性氧化铝、大流量短历时的NaOH循环再生法等新技术,提高了活性氧化铝的吸附容量和处理水的能力,克服滤料板结和假疲劳,除氟效果稳定可靠,水质好,再生时间短,用药省,降低了基建投资和运转费用。 | North China Municipal Engineering Design Institute A full-scale plant was set up for studying the existing problems on the fluoride removal from drinking water by activated alumina. Some new methods was adopted,such as the addition of CO2to reduce water pH value,the use of small-sized activated alumina,and the employment of a cyclic regeneration at large flow rate with NaOH as regenerant,which result in the improvement of adsorption capacity of activated alumina and treatment capacity, the elimination of the hardening of filtration materials and false fatigue,stable and reliable effectiveness,good quality in water,short regeneration time,small consumption of regenerant. And then the capital and openational costs were reduced. | 1989 | 中国给水排水1989，5，3： | 5 |
| 3 | The Cosmic Dust in Suzhou Granite:Ablated Products of a Cometary Explosion | Hu Zhong-wei,Wang Er-kang,Wan Yu-qiuDepartement of Astronomy,Nanjing University,Nanjing 210093,China Department of Earth Sdence,Nanjing University,Nanjing 210093,China Cosmic dust has been collected in large number from deap-sea sediments,Greenland ice deposits,the stratosphere and space,of cosmic dust,some are the unmelted micrometeorite,and most are the ablated products of me-teoroids in the atmosphere.Cosmic dusts were recovered from two sites of the 1908 Tunguska explosion and the 1965 Revelstoks explosion Since 1987,a large number of microspherules have been extracted from Suzhou granite of China Their suface and section structures,mineral and chemical composition show that these microspherules are of the ablated cosmic dust.The microspherules occurred in the inner contact complex intrusion.It suggests that the microspherules may be products of the same event and were aggergated in some strata and then captured by the emplacement of granitic magma.Although the microspherules are similare to other ablated cosmic dusts,there are some diferences.Our calculated results of cometary motion through the atmosphere and explosion show that the microspherules may be the ablated dust of a comet with mass above 1015g at its explosion near the ground. | 1996 | 紫金山天文台台刊1996，15，3： | 2 |
| 4 | Chaperones and protein folding in the archaea显示文摘 | Large A T Goldberg M D Lund P A | 2009 | Biochem Soc Trans2009，37，1： | 1 |
| 5 | Reach-scale sediment transfers: An evaluation of two morphological budgeting approaches显示文摘 | Fuller I C Large A R G Charlton M E | 2003 | Earth Surface Processes and Landforms2003，28，8： | 1 |
| 6 | Characteristics of homicide offenders with Schizophrenia from the Russian Federation显示文摘 | Golenkov A Large M Nielssen O | | 0，，1： | 1 |
| 7 | Multilayer Reflectors in Animals Using Green and Gold Beetles as Con- trasting Examples显示文摘 | PARKER A R MCKENZIE D R LARGE M C J | 1998 | J Exp Biol1998，201，： | 1 |
| 8 | Chaperones and protein folding in the archaea 显示文摘 | Large A T Goldberg M D Lund P A | 2009 | Biochena Soe Trans2009，37，： | 1 |
| 9 | Gender differences in collaborative Web searching behavior:an elementary school study显示文摘 | Large A L Beheshti J Rahman T | 2002 | Information Processing and Management2002，38，3： | 1 |
| 10 | The danger of dangerousness : why we must remove the dangerousness criterion from our mental health acts 显示文摘 | LARGE M M RYAN C J NEII-SSEN O B HAYES R A | 2008 | Journal of Medical Ethics2008，34，12： | 1 |
| 11 | Another tool in the anterolateral thigh's box显示文摘 | ABOOD A HALVORSON E PUSIC A A modified 'paddle-flap' for reconstruction of a large pharyngo cutaneous fistula and anterior neck defect | 2008 | J Plast Reconstr Aesthet Surg2008，1，： | 1 |
| 12 | Low thresholdquasi-three-level 946 nm laser operation of an epitaxiallygrown Nd∶Y3Al5O12waveguide显示文摘 | Hanna D C Large A C Shepherd D P | 1993 | Appl Phys Lett1993，63，1： | 1 |
| 13 | High dynamic range characterization of the trauma patient plasma proteome显示文摘 | Liu T Qian W J Gritsenko M A Xiao W Moldawer L L Kaushal A Monroe M E Vamum S M Moore R J Purvine S O Maier R V Davis R W Tompkins R G Camp D G 2nd Smith R D and the Inflammation and the Host Response to Injury Large Scale Collaborative Research Programm | | 0，，10： | 1 |
| 14 | Low threshold ion-implanted Nd∶YAG channel waveguide laser显示文摘 | Field S J Hanna D C Large A C | 1991 | ElectronLett1991，27，： | 1 |
| 15 | Children in the information technology design process: A review of theories and their applications显示文摘 | Nesset V Large A | 2004 | Library & Information Science Research2004，，26： | 1 |
| 16 | Sex differences in visceral fat lipolysis and metabolic complications of obesity显示文摘 | LONNQV1ST F THORNE A LARGE V | 1997 | Arterioscler Thromb Vasc Biol1997，17，： | 1 |
| 17 | Gender differences in collaborative Web searching behavior:An elementary school study显示文摘 | Large A Beheshti J Rahman T | | 0，，3： | 1 |
| 18 | Airway stenoses after lung transplantation:management with expanding metal stents 显示文摘 | Higgins R McNeil K Dennis C Parry A Large S Nashef S A | 1994 | J Heart Lung Transplant1994，13，： | 1 |
| 19 | Microstructured polymer optical fibre 显示文摘 | Van eijkelenborg M A Large M C J Argyros A | 2001 | Optics Express2001，，9： | 1 |
| 20 | Chaperones and protein folding in the archaea 显示文摘 | Large A T Goldberg M D Lurid P A | 2009 | Biochem Soc Trans2009，37，1： | 1 |
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