|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The expression of hTERT mRNA and cellular immunity in gastric cancer and precancerosis显示文摘AIM:To observe the expression of Human telomerasereverse transcriptase (hTERT) in gastric carcinomas andprecancerosis lesions, to evaluate the immune state ofsuch patients, and to then study the clinical significanceof hTERT and immune state for the diagnosis, treat-ment and prognosis of gastric cancer METHODS: In situ hybridization was used to detect theexpression of hTERT mRNA in 116 endoscopic of gas-tric mucosa. Analyzed tissue samples were as follows:30 cases of chronic superficial gastritis (CSG), 44 ofprecancerosis lesions (including 27 of chronic atrophicgastritis, 8 of adenomatous polyp and 9 of gastric ulcer)and 42 of gastric cancer (GC). In addition, the T lym-phocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+ ) andnatural killer cells (NK) in peripheral blood were deter-mined by flow cytometric analysis (FCM) in 30 cases ofCSG, 27 of precancorosis (chronic atrophic gastritis,CAG), and 42 of GC. The data were compared with thoseof normal control (NC).RESULTS:The detected positive rate of hTERT varied asfollows: 86 % (36/42) in GC, 36 % (16/44) inprecancerosis lesions and 0 % (0/30) in CSG. The ex-pression of hTERT mRNA was not associated with pa-tient gender, tumor location, macroscopic type, lymphnode metastasis, or degree of differentiation, It wasfound that the CD3+, CD4+ of the CSG group were lowerthan that of NC (P<0.05). Meanwhile, the T lympho-cyte subsets (CD3+,CD4+, CD4+/CD8+ ratio) and NK cellsof CAG were remarkably lower than that of NC and CSGgroups (P<0.05-0.01). Values ofT cells and NK cells ofthe GC group were significantly abnormal when com-pared with the CAG group (P<0.05-0.01). Furthermore,with tumor progression, the function of T cells wasweakened gradually.CONCLUSION: The expression of telomerase may be acrucial step in gastric carcinogenesis and increasedhTERT mRNA may serve as a novel marker for diagno-sis of GC. The immune state of patients with GC andprecancerosis was somewhat depressed, which indi-cates the importance of cellular immunological assaysin cancer patients. | Xi-Xian Yao Lei Yin Department of Digestive Medicine,the 2nd Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China Zhong-Cheng Sun The Traditional Chinese Medical College of Hebei Medical University,Shijiazhuang 050081,Hebei Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 32 |
| 2 | Aoyitake plagiogranite in western Tarim Block, NW China: Age, geochemistry, petrogenesis and its tectonic implications显示文摘SHRIMPP U-Pb zircon age and geochemical and Nd isotopic data are reported for the Aoyitake plagiogranite in western Tarim Block, NW China. The plagiogranite intruded the Middle Pro- terozoic and Lower Carboniferous with an exposure area of ca. 60 km2 and crystallized at 330.7±4.8 Ma. Rock types mainly include tonalite, trondhjemite and minor amounts of diorite and quartz-diorite. Feldspars in the rocks are dominated by oligoclase-andesine, and minor perthite observed locally. The granites are sodic with Na/K ratios (molar) between 4 and 87. Total REE (50-220 ppm) show a clear positive correlation with SiO2. There is no LRRE/HREE fractionation (LaN/YbN=0.5-1.5), me- dium negative Eu anomalies (δ Eu=0.3-0.6), high Y content and low Sr/Y ratio (~1.0). These granites exhibit relatively juvenile Nd T2DM model ages of 470 to 580 Ma and positive εNd(331 Ma) values of 6.23 to 7.65. The aforementioned characteristics are similar to those of ocean island or ocean ridge plagiogranites. However, the regional geology, especially its scale, precludes that the plagiogranite pluton was derived directly from fractionational crystallization of mantle-derived basaltic magma. We interpreted that the primary magma of the pluton might be tonalitic in composition generated by ca. 50% partial melting of the juvenile basaltic crust. The primary magma experienced intensive frac- tionational crystallization, and intruded into the middle to upper crusts to form the granite pluton. In combination with the previous regional geological data, it is concluded that the plagiogranite pluton was emplaced within the Tarim Block in respond to the Carboniferous continental rifting along the Tianshan orogenic belt. | ZHANG Chuanlin1,2, YU Haifeng3, YE Haimin2, ZHAO Yu2 & ZHANG Dongsheng4 1. Key Laboratory of Isotope Geochronology and Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China 2. Nanjing Institute of Geology and Mineral Resources, Nanjing 210016, China 3. Tianjin Institute of Geology and Mineral Resources, Tianjin 300170, China 4. The 2nd Regional Geological Investigation Party, Xinjiang Bureau of Geology and Mineral Resources, Changji 831100, China | 2006 | Science China Earth Sciences2006，49，11： | 11 |
| 3 | Oxidative Stress before and after Operation in Patients with Chronic Cholecystitis Containing Gallstone显示文摘To study the relationship of oxidative, antioxidative constituents and antioxidases in blood with chronic cholecystitis containing gallstone, levels of lipoperoxides (LPO), nitric oxide (NO), vitamin C(VC), vitamin E (VE) and β carotene (β CAR) in plasma as well as level of LPO, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) in erythrocytes were investigated by spectrophotometric assay in 107 patients with this condition (PCg) and 100 healthy volunteers (HVs). Compared with HVs group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of PCg group were significantly increased ( P <0.0001), while that of VC, VE and β CAR in plasma and the average activities of SOD, CAT and GSH Px in erythrocytes were significantly decreased ( P <0.0001). Linear regression and correlation analysis for 107 preoperative PCg showed that the value of LPO and NO in plasma and that of LPO in erythrocytes of PCg gradually increased ( P <0.0001), representing a significant linear positive correlation. The value of VC, VE and β CAR in plasma and that of SOD, CAT and GSH Px in erythrocytes of PCg gradually decreased ( P <0.0001), representing a significant linear negative correlation. Stepwise regression and correlation analysis for 107 preoperative PCg suggested that the closest correlation was observed between the course of disease and the value of NO and VC in plasma and that of SOD, GSH Px and LPO in erythrocytes, r =0.7306, F =32.1408, P <0.0001. Compared with the preoperative PCg group, the average value of LPO and NO in plasma and that of LPO in erythrocytes of the postoperative PCg group were significantly decreased ( P <0.0001). Furthermore, the average value of VC in plasma and that of SOD, CAT and GSH Px in erythrocytes of the postoperative PCg group were significantly increased ( P <0.0001), whereas no significant difference was found between their average value of VE and β CAR in plasma. These findings suggested that oxidative stress was an aggravating pathological condition in PCg group. Therefore, we recommend that in treating PCg, antioxidants such as VC, VE, β CAR should be given in order to alleviate their potential oxidative damages. | ZHOU JUN FU , CHEN PENG , YANG JIN LU +, ZHU YOU GEN + PENG CHENG HONG , AND WU YU LIAN The 2nd Affiliated Hospital, Medical College of Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejian | 2000 | Biomedical and Environmental Sciences2000，13，4： | 10 |
| 4 | Analysis for phenotype of HNPCC in China显示文摘AIM: The aims of this study were to identify theclinicopathological features of Chinese HNPCC families andto evaluate the value of criteria for suspected HNPCC(sHNPCC) in clinical diagnosis.METHODS: According to the follow-up records, 54 HNPCCfamilies (including 12 TCG-HNPCC families and 42 sHNPCCfamilies) were screened out from patients with colorectal cancers(CRCs), operated upon in 2nd Affiliated Hospital of ZhejiangUniversity from 1984 to 2001. Clinical data of probands andtumor spectrum in these families were listed and analyzed,RESULTS: (1) Mean age, proportion of colonic cancer, poorlydifferentiated cancer, multiple CRCs and Dukes′ A+B of theprobands in ICG-HNPCC and sHNPCC kindred were 39ys and47.5ys, 75 % and 62 %, 0 and 12.8 %, 16.7 % and 14.3 %,58.3 % and 81%,respectively. Compared with sporadiccolorectal cancers, probands from ICG-HNPCC and sHNPCCfamilies were obviously different at age of onset(P=0.025and 0.031), tumor location (P=0.001 and 0.000),differentiation(P=0.002 and 0.011) and development ofmultiple tumors (P=0.014 and 0.002). (2) A total of 178malignant neoplasms were found in 54 HNPCC families,including 139 colorectal cancers. Besides of colorectal cancer,extracolonic tumors occurred in stomach, endometrium,hepatobiliary system, and so on (8 gastric cancers, 6 endometrialcancers, 6 hepatobiliary system cancers and 19 others) canalso be seen in Chinese ICG-HNPCC and sHNPCC families.CONCLUSION: (1) Chinese HNPCC families have specificclinicopathological features, such as early onset, predilectionfor the involvement of colon, tendency of multiple CRCs,development of extracolonic tumors and well differentiation.(2) The criteria for suspected HNPCC is useful in clinicaldiagnosis and management of HNPCC. | Yong-Mao Song Department of Oncology,2nd Affiliated Hospital,Medical College,Zhejiang University,Hangzhou 310009,Zhejiang Province,China Shu Zheng Cancer Institute,Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 5 |
| 5 | 龙窝湖斜颌鲴生态调查报告显示文摘斜颌鲴是一种重要的野生经济鱼类,分布于我国黑龙江、长江、珠江流域(伍献文等。《中国鲤科鱼类志》上卷)。 | Fisheries Administrative Board of Lake Lungwohu,Fanchang County,Anhwei Province Fisheries Administrative Station of Wuhu District,Anhwei Province Section of Introduction and Acclimation,the 2nd Laberatory,Institute of Hydrobiology,Hupei Province | 1977 | 水生生物学集刊1977，6，2： | 4 |
| 6 | Zeus robot-assisted laparoscopic cholecystectomy in comparison with conventional laparoscopic cholecystectomy显示文摘BACKGROUND: The robotic surgical system overcomes many technological obstacles of conventional laparoscopic surgery, and possesses enormous clinical applied potential. The aim of this study was to compare the efficacy of Zeus robot-assisted laparoscopic cholecystectomy with conventional laparoscopic cholecystectomy. METHODS: Forty patients undergoing elective cholecystectomy were randomly divided into two groups. Patients in group A (n=20) underwent Zeus robot-assisted laparoscopic cholecystectomy, and patients in group B (n=20) received conventional laparoscopic cholecystectomy. The parameters on operative field, operative time, the number of actions, the rate of operative errors and minimal trauma were evaluated and compared between the two groups. RESULTS: The number of cleating camera (1.1±1.0 times) and the time of adjusting the operative field (2.2±0.7 minutes) in group A were significantly less than those (4.5±1.5 times) and (7.5±1.2 minutes) in group B. The number of dissection actions (337±86 times) and the rate of operative errors (10%) in group A were less than those (389±94 times), (25%) in group B. The total operation time (104.9±20.5 minutes) and setup time (29.5±9.8 minutes) in group A were significandy longer than those (78.6±17.1 minutes), (12.6±2.5 minutes) in group B. Blood loss and postoperative hospitalization were similar. No postoperative complications occurred in both groups, and open cholecystectomy was performed in each group. CONCLUSIONS: Zeus robot-assisted cholecystectomy inherits the benefits of minimally invasive surgery. The Zeus robotic surgical system is better than conventional laparoscopic technique in controlling the operative field and can be manipulated precisely and stablely though it requires more operative time. | Han-Xin Zhou, Yue-Hua Guo, Xiao-Fang Yu, Shi-Yun Bao, Jia-Lin Liu, Yue Zhang and Yong-Gong Ren Department of Minimally Invasive Surgery, Shenzhen People’s Hospital, Jinan University 2nd Clinical Medicine College, Shenzhen 518020. China Department of Anesthesia, Shenzhen People’s Hospital, Jinan University 2nd Clinical Medicine College, Shenzhen 518020, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 3 |
| 7 | 重组脊髓灰质炎病毒用于治疗复发胶质母细胞瘤(英文)显示文摘Background The prognosis of patients with recurrent World Health Organization(WHO)grade IV malignant glioma is dismal,and there is currently no effective therapy.We conducted a dose-finding and toxicity study in this population of patients,evaluating convection-enhanced,intratumoral delivery of the recombinant nonpathogenic polio-rhinovirus chimera(PVSRIPO).PVSRIPO recognizes the poliovirus receptor CD155,which is widely expressed in neoplastic cells of solid tumors and in major components of the tumor microenvironment.Methods We enrolled consecutive adult patients who had recurrent supratentorial WHO grade IV malignant glioma,confirmed on histopathological testing,with measurable disease(contrast-enhancing tumor of≥1 cm and≤5.5 cm in the greatest dimension).The study evaluated seven doses,ranging between 107 and 1010 50%tissue-culture infectious doses(TCID50),first in a dose-escalation phase and then in a dose-expansion phase.Results From May 2012 through May 2017,a total of 61 patients were enrolled and received a dose of PVSRIPO.Dose level-1(5.0×107TCID50)was identified as the phase 2 dose.One dose-limiting toxic effect was observed;a patient in whom dose level 5(1010TCID50)was administered had a grade 4 intracranial hemorrhage immediately after the catheter was removed.To mitigate locoregional inflammation of the infused tumor with prolonged glucocorticoid use,dose level 5 was deescalated to reach the phase 2 dose.In the dose-expansion phase,19%of the patients had a PVSRIPO-related adverse event of grade 3 or higher.Overall survival among the patients who received PVSRIPO reached a plateau of 21%(95%confidence interval,11 to 33)at 24 months that was sustained at 36 months.Conclusions Intratumoral infusion of PVSRIPO in patients with recurrent WHO grade IV malignant glioma confirmed the absence of neurovirulent potential.The survival rate among patients who receivedPVSRIPO immunotherapy was higher at 24 and 36 months than the rate among historical controls. | Desjardins A Gromeier M Herndon JE 2nd Beaubier N Bolognesi DP Friedman AH Friedman HS McSherry F Muscat AM Nair S Peters KB Randazzo D Sampson JH Vlahovic G Harrison WT McLendon RE Ashley D Bigner DD | 2018 | 中华神经外科疾病研究杂志2018，17，4： | 2 |
| 8 | 人乳头瘤病毒与鼻咽癌显示文摘目前,关于人乳头瘤病毒(HPV)与鼻咽癌(NPC)相关性的研究报道较少。该文作者通过检索国家癌症数据库(NCDB)的资料,对2004-2013年间的数据进行回顾分析,采用逻辑回归方法检测HPV阳性患者的相关因素,Kaplan-Meier法评估HPV+/-患者与总生存率(OS)的相关性。结果 :纳入研究的956例患者中,32%HPV+,68%HPV-。 | Verma V Simone CB 2nd Lin C 张凌 | 2018 | 中国口腔颌面外科杂志2018，16，2： | 2 |
| 9 | 术中应用荧光素钠有利于胶质母细胞瘤浸润边缘的手术切除(英文)显示文摘Objective Extent of resection is an important prognostic factor in patients undergoing surgery for glioblastoma( GBM).Recent evidence suggests that intravenously administered fluorescein sodium associates with tumor tissue,facilitating safe maximal resection of GBM. In this study,the authors evaluate the safety and utility of intraoperative fluorescein guidance for the prediction of histopathological alteration both in the contrast-enhancing( CE) regions,where this relationship has been established,and into the nonCE( NCE),diffusely infiltrated margins. Methods Thirty-two patients received fluorescein sodium( 3 mg/kg) intravenously prior to resection. Fluorescence was intraoperatively visualized using a Zeiss Pentero surgical microscope equipped with a YELLOW 560 filter.Stereotactically localized biopsy specimens were acquired from CE and NCE regions based on preoperative MRI in conjunction with neuronavigation. The fluorescence intensity of these specimens was subjectively classified in real time with subsequent quantitative image analysis,histopathological evaluation of localized biopsy specimens,and radiological volumetric assessment of the extent of resection.Results Bright fluorescence was observed in all GBMs and localized to the CE regions and portions of the NCE margins of the tumors,thus serving as a visual guide during resection. Gross-total resection( GTR) was achieved in 84% of the patients with an average resected volume of 95%,and this rate was higher among patients for whom GTR was the surgical goal( GTR achieved in 93. 1% of patients,average resected volume of 99. 7%). Intraoperative fluorescein staining correlated with histopathological alteration in both CE and NCE regions,with positive predictive values by subjective fluorescence evaluation greater than 96% in NCE regions. Conclusions Intraoperative administration of fluorescein provides an easily visualized marker for glioma pathology in both CE and NCE regions of GBM. These findingssupport the use of fluorescein as a microsurgical adjunct for guiding GBM resection to facilitate safe maximal removal. | Neira JA Ung TH Sims J Malone HR Chow DS Samanamud JL Zanazzi GJ Guo X Bowden SG Zhao B Sheth SA McKhann GM 2nd Sisti MB Canoll P D'Amico RS Bruce JN | 2016 | 中华神经外科疾病研究杂志2016，15，5： | 2 |
| 10 | Hepatitis gene chip in detecting HBV DNA, HCV RNA in serum and liver tissue samples of hepatitis patients显示文摘OBJECTIVE: To study the preparation of diagnostic gene chip for detecting hepatitis B virus (HBV) andhepatitis C virus (HCV) and its accuracy in detecting HBV DNA and HCV RNA in serum and liver tissues.METHODS: The probes, which depend on the conservative gene fragment of hepatitis virus, wasdesigned, synthesized and spotted on the modified glass. The probes and some other control probes wereassembled on the diagnostic microarray of hepatitis virus. The gene of hepatitis virus, purified from bloodor tissue, was labeled with fluorescence and hybridized to the microarray. The hybridized microarry wasscanned with microarray scanner and the diagnostic result was analyzed from the scanning data. Fourtypatients with hepatitis B virus and 40 healthy people or 40 patients with hepatitis C virus were subjected todetection of HBV DNA and HCV RNA with the hepatitis virus gene chip by the double-blind method.Paraffin liver specimens obtained from 99 cases of posthepatitic cirrhosis were used to detect HBV DNA.The liver tissues and serum from 15 cases of chronic hepatitis B were used to detect HBV DNA.Simultaneously, HBsAg and HBcAg were detected in the serum by fluorescence microparticlequantitation, HBV DNA and HCV RNA in the serum by PCR, and HBcAg in liver tissues byimmunocytochemistry or HBV DNA by in situ molecular hybridization.RESULTS: Chip detection of serum specimens showed that 30 patients were HBV DNA positive and 10HBV DNA negative in the 40 patients with HBV positive, 25 patients were HCV RNA positive and 15patients were HCV RNA negative in the 40 patients with HCV positive, and all were HBV and HCVnegative in the 40 healthy people. In 15 patients with HBV marker positive who were subjected to liverbiopsy, 15 patients were detected HBV DNA positive in serum by gene chip, 15 patients HBcAg positivein liver tissues by immunocytochemistry, 14 patients HBV DNA positive in liver tissues by in situmolecular hybridization, and 14 patients HBV DNA positive in liver tissues by gene chip. Paraffin livertissues specimens from the 99 patients with posthepatitis B cirrhosis showed that 67 patients were detectedHBcAg positive by immunocytochemistry, 53 patients HBV DNA positive by in situ molecularhybridization, and 46 patients HBV DNA positive by gene chip. In the 46 patients, 40 patients weredetected HBV DNA and HBcAg positive by in situ molecular hybridization and immunocytochemistry, 6patients only HBcAg positive, and 33 patients HBcAg negative.CONCLUSIONS: The designed diagnostic gene chip can be used to simultaneously detect serum HBVDNA and HCV RNA, but the positive rate of HCV RNA diagnosed by this chip is lower. The gene chipcan detect HBV DNA in serum and in liver tissue. | Wei Zhao Jian-Min Wan Wei Liu Quan-Jun Liu Lin Zhang Zhen-Xian Zhou Xin-Jue Liu Han-Rong Zhang the College of Biological Science and Technology, Nanjing Agriculture University, Nanjing 210014, China Department of Liver Disease Research, Nanjing 2nd Hospital, Southeast University. Nanjing 210003, China National Laboratory for Molecular and Biomolecular Electronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，2： | 2 |
| 11 | Flexural strength of feldspathic porcelain treated with ion exchange, overglaze and polishing显示文摘 | Russell A Giordano R A 2nd Campbell S | 1994 | J Prosthet Dent1994，71，： | 2 |
| 12 | 斜颌鲴的食性研究显示文摘斜颔鲴在我国分布很广,它栖息在水体的中下层,并常以其较发达的下颔角质边缘在石面或泥表刮取食物。据有关资料记载,斜颔鲴是以水生高等植物的枝叶及硅藻、丝状藻类为主要食物(伍献文等,《中国鲤科鱼类志》上卷)。在乌苏里江、兴凯湖、松花江等水域中,其主要食物为腐植质、有机碎屑(以下简称腐屑) | Section of Introduction and Acclimation,the 2nd Laboratory,Institute of Hydrobiology,Hupei Province | 1977 | 水生生物学集刊1977，6，2： | 2 |
| 13 | Matrix Metalloproteinases and Their Role in Pancreatic Cancer:a Review of Preclinical Studies and Clinical Trials显示文摘 | Bloomston M Zervos EE Rosemurgy AS 2nd | 2002 | Ann Surg Oncol2002，9，7： | 1 |
| 14 | Lung infections:role of apoptosis in host defense and pathogenesis of disease显示文摘 | Behnia M Robertson KA Martin W J 2nd | 2000 | Chest2000，117，6： | 1 |
| 15 | Optimal perfusion during cardiopulmonary bypass: an evidence based approach 显示文摘 | Murphy GS Hessel EA 2nd Groom RC | 2009 | Anesth Analg2009，108，5： | 1 |
| 16 | Retrospective review of 208 patients with leiomyosarcoma of the uterus : prognostic indicators, surgical management, and adjuvant therapy 显示文摘 | Giuntoli RL 2nd Metzinger DS DiMarco CS | 2003 | Gynecol Oncol2003，89，3： | 1 |
| 17 | Accuracy of a novel automatic atrial capture threshold algorithm显示文摘 | Adler S 2nd Schaerf R Quan KJ | 2003 | PACE2003，26，： | 1 |
| 18 | NF- kappaB promotes breast cancer cell migration and metastasis by inducing the expression of the chemokine receptor CXCR4 显示文摘 | Helbig G Christopherson KW 2nd Bhat-Nakshatri P | 2003 | J Biol Chem2003，278，21： | 1 |
| 19 | IL-17 contributes to angiogenesis in rheumatoid arthritis显示文摘 | Pickens S R Volin M V Mandelin A M 2nd | 2010 | J Immunol2010，184，6： | 1 |
| 20 | Supracondylar frac-ture of the adult femur:a study of one hundred and ten ca-ses显示文摘 | Neer CS 2nd Grantham SA Shelton ML | 1967 | J Bone Joint Surg Am1967，49，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员