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1Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice显示文摘Shi-Jiang Lu Feng Li Hong Yin Qiang Feng Erin A Kimbrel Eunsil Hahm Jonathan N Thon Wei Wang Joseph E ltaliano Jaehyung Cho Robert Lanza 2011Cell Research2011,21,3:14
2Quantitative Trait Loci Mapping of Maize Yield and Its Components Under Different Water Treatments at Flowering Time显示文摘干旱或水应力是导致玉米(Zea mays L.) 的一个严重农学的问题在全世界的收益损失。与干旱忍耐引起混血儿是为解决这个问题的一条重要途径。然而,更低的效率和引起混血儿的一个更长的时期是传统的繁殖节目的劣势。到传统的繁殖节目的适用的分子的标记技术能改进繁殖干旱容忍的玉米的效率,这通常被认出。在玉米干旱忍耐的研究为使用提供有用信息,为产量和它的部件印射并且标注量的特点 loci (QTL ) 根据一个混合线性模型的原则在现在的学习被执行。221 recombinant Yuyu 22 的生来的线(RIL ) 在浇得好、强调水的条件下面被种。在以前的处理组,植物很好被灌溉,而那些在 flowering 时间在后者处理组被强调。每遗传型的十植物连续不断地被种那是 3.00 m ??? 隤? ???? ? 跧?? 闦?? ? 郦 ?? ? 釧 ?  ? 跧? 釧??? 雧???????? 雧? 吗??Gui-He Lu Ji-Hua Tang Jian-Bing Yan Xi-Qing Ma Jian-Sheng Li Shao-Jiang Chen Jian-Cang Ma Zhan-Xian Liu Li-Zhu E Yi-Rong Zhang Jing-Rui Dai 2006Journal of Integrative Plant Biology2006,48,10:13
3A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China.Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu 2006Cellular & Molecular Immunology2006,3,6:9
4An implementation assessment of China's Environmental Information Disclosure Decree显示文摘China's 2007 Open Government Information Regulations is widely considered as a milestone in the country's information policy history and is praised as a'sunshine program'.The Environmental Information Disclosure Decree was the first to operationalize these general regulations into a sectoral information disclosure system on environment.This study assessed the implementation of the environmental information disclosure system about six months after the Decree took eect on May 1,2008.Through reviewing the websites of all 31 provincial Environmental Protection Bureaus and the Ministry of Environmental Protection,conducting an experiment with actual information disclosure request,and through interviews with all provincial Environmental Protection Bureaus,this article concluded that the implementation of the environmental information disclosure system still falls short.Future improvements should focus on further publicity of the regulations and decree to enhance public participation,the establishment of an independent evaluation and supervision system for information disclosure,the exchange of experiences and best practices among provincial Environmental Protection Bureaus,and on strengthening the legal status of environmental information disclosure.Lei Zhang Arthur E J Mol Guizhen He Yonglong Lu 2010Journal of Environmental Sciences2010,22,10:9
5Changes in retinal and choroidal morphology after cerebrospinal fluid pressure reduction: a Beijing iCOP study显示文摘Dear Editor,Glaucoma is a multifunctional neurodegenerative disease and is the leading cause of irreversible blindness. It is characterized by progressive loss of retinal ganglion cells and their axons leading to visual field defects.Lu Liu Xiaoxia Li Hanspeter E Killer Kai Cao Jing Li Ningli Wang 2019Science China(Life Sciences)2019,62,2:7
6汽车采用的无级变速功率分流变速器的设计显示文摘在汽车应用方面提出了无级变速功率分流变速箱的一种设想 ,该装置由两可变速带轮 (变速器 )和一行星齿轮系所谓太阳轮和内齿圈三转动构件组合而成。“功率分流”的布置特点是输入轴同时把功率传到太阳轮和主动带轮 ,并通过变速器传到行星齿轮传动的内齿圈。因为输入轴传递的输入功率一分为二 (传到太阳轮和主动带轮 ) ,变速器中仅通过输入轴总功率流的一小部分 ,行星齿轮传动的第三转动件 (转臂 )收集了由内齿圈和太阳轮传来的功率流 ,把总功率传到输出轴传到车辆。该特徵提高了发动机功率在汽车应用的许可范围 ,该特徵特别在低速 -高转矩情况下提供无级变速可变传动比的范围时还降低了与动力传动有关的功率损失。建立一个所谓“增益系数”,用它通过变速箱的可变速元件和速比间隔来确定总功率流的两个分量的大小。用一外齿轮箱可以在发动机和变速箱的最佳范围内扩展无级变速范围。提出了一个采用外啮合有级齿轮传动 (同步变速器 )实现有效传动比间隔的无级变速范围应用于汽车的设计程序 。V H Mucino Z Lu J E Smith M Kimcikiewicz B Cowan 2004传动技术2004,18,1:7
7Comparison of non-schistosomal rectosigmoid cancer and schistosomal rectosigmoid cancer显示文摘AIM: To compare the clinicopathological features of patients with non-schistosomal rectosigmoid cancer and schistosomal rectosigmoid cancer.METHODS: All the patients with rectosigmoid carcinoma who underwent laparoscopic radical surgical resection in the Shanghai Minimally Invasive Surgical Center at Ruijin Hospital affiliated to Shanghai JiaoTong University between October 2009 and October2013 were included in this study. Twenty-six cases of colonic schistosomiasis diagnosed through colonoscopy and pathological examinations were collected. Symptoms,endoscopic findings and clinicopathological characteristics were evaluated retrospectively.RESULTS: There were no significant differences between patients with and without schistosomiasis in gender, age, CEA, CA19-9, preoperative biopsy findings or postoperative pathology. Patients with rectosigmoid schistosomiasis had a significantly higher CA-125 level and a larger proportion of these patients were at an early tumor stage(P = 0.003). Various morphological characteristics of schistosomiasis combined with rectosigmoid cancer could be found by colonoscopic examination: 46% were fungating mass polyps, 23%were congestive and ulcerative polyps, 23% were cauliflower-like masses, 8% were annular masses.Only 27% of the patients were diagnosed with rectal carcinoma preoperatively after the biopsy. Computed tomography(CT) scans showed thickened intestinal walls combined with linear and tram-track calcifications in 26 patients.CONCLUSION: Rectosigmoid carcinoma combined with schistosomiasis is associated with higher CA-125 values and early tumor stages. CA-125 and CT scans have a reasonable sensitivity for the accurate diagnosis.Hao Feng Ai-Guo Lu Xue-Wei Zhao Ding-Pei Han Jing-Kun Zhao Lei Shi Tobias S Schiergens Serene ML Lee Wen-Peng Zhang Wolfgang E Thasler 2015World Journal of Gastroenterology2015,21,23:6
8Toxicity of Graphene Quantum Dots in Zebrafish Embryo显示文摘Objective To evaluate the bio-safety of graphene quantum dots(GQDs), we studied its effects on the embryonic development of zebrafish. Methods In vivo, biodistribution and the developmental toxicity of GQDs were investigated in embryonic zebrafish at exposure concentrations ranging from 12.5-200 μg/m L for 4-96 h post-fertilization(hpf). The mortality, hatch rate, malformation, heart rate, GQDs uptake, spontaneous movement, and larval behavior were examined. Results The fluorescence of GQDs was mainly localized in the intestines and heart. As the exposure concentration increased, the hatch and heart rate decreased, accompanied by an increase in mortality. Exposure to a high level of GQDs(200 μg/mL) resulted in various embryonic malformations including pericardial edema, vitelline cyst, bent spine, and bent tail. The spontaneous movement significantly decreased after exposure to GQDs at concentrations of 50, 100, and 200 μg/mL. The larval behavior testing(visible light test) showed that the total swimming distance and speed decreased dose-dependently. Embryos exposed to 12.5 μg/mL showed hyperactivity while exposure to higher concentrations(25, 50, 100, and 200 μg/mL) caused remarkable hypoactivity in the light-dark test. Conclusion Low concentrations of GQDs were relatively non-toxic. However, GQDs disrupt the progression of embryonic development at concentrations exceeding 50 μg/mL.WANG Zhen Guo ZHOU Rong JIANG Dan SONG Jing E XU Qian SI Jing CHEN Yun Ping ZHOU Xin GAN Lu LI Jian Zhen ZHANG Hong LIU Bin 2015Biomedical and Environmental Sciences2015,28,5:6
9Texture analysis on parametric maps derived from dynamic contrast-enhanced magnetic resonance imaging in head and neck cancer显示文摘AIM: To investigate the merits of texture analysis on parametric maps derived from pharmacokinetic modeling with dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI) as imaging biomarkers for the prediction of treatment response in patients with head and neck squamous cell carcinoma(HNSCC). METHODS: In this retrospective study,19 HNSCC patients underwent pre- and intra-treatment DCEMRI scans at a 1.5T MRI scanner. All patients had chemo-radiation treatment. Pharmacokinetic modeling was performed on the acquired DCE-MRI images,generating maps of volume transfer rate(Ktrans) and volume fraction of the extravascular extracellular space(ve). Image texture analysis was then employed on maps of Ktrans and ve,generating two texture measures: Energy(E) and homogeneity.RESULTS: No significant changes were found for the mean and standard deviation for Ktrans and ve between pre- and intra-treatment(P > 0.09). Texture analysis revealed that the imaging biomarker E of ve was significantly higher in intra-treatment scans,relative to pretreatment scans(P < 0.04). CONCLUSION: Chemo-radiation treatment in HNSCC significantly reduces the heterogeneity of tumors.Jacobus FA Jansen Yonggang Lu Gaorav Gupta Nancy Y Lee Hilda E Stambuk Yousef Mazaheri Joseph O Deasy Amita Shukla-Dave 2016World Journal of Radiology2016,8,1:5
10Evaluation of a scientific CMOS camera for astronomical observations显示文摘We evaluate the performance of the first generation scientific CMOS (sC-MOS) camera used for astronomical observations. The sCMOS camera was attached to a 25 cm telescope at Xinglong Observatory, in order to estimate its photometric capabilities. We further compared the capabilities of the sCMOS camera with that of full-frame and electron multiplying CCD cameras in laboratory tests and observations. The results indicate the sCMOS camera is capable of performing photometry of bright sources, especially when high spatial resolution or temporal resolution is desired.Peng Qiu Yong-Na Mao Xiao-Meng Lu E Xiang Xiao-Jun Jiang 2013Research in Astronomy and Astrophysics2013,13,5:4
11Silvicultural alternatives to conventional even-aged forest man-agement-what limits global adoption?显示文摘Background: The development of forestry as a scientific and management discipline over the last two centuries has mainly emphasized intensive management operations focused on increased commodity production, mostly wood. This 'conventional' forest management approach has typically favored production of even-aged, single-species stands. While alternative management regimes have generally received less attention, this has been changing over the last three decades, especially in countries with developed economies. Reasons for this change include a combination of new information and concerns about the ecological consequences of intensive forestry practices and a willingness on the part of many forest owners and society to embrace a wider set of management objectives. Alternative silvicultural approaches are characterized by a set of fundamental principles, including avoidance of clearcutting, an emphasis on structural diversity and small-scale variability, deployment of mixed species with natural regeneration,and avoidance of intensive site-preparation methods.Methods: Our compilation of the authors' experiences and perspectives from various parts of the world aims to initiate a larger discussion concerning the constraints to and the potential of adopting alternative silvicultural practices.Results: The results suggest that a wider adoption of alternative silvicultural practices is currently hindered by a suite of ecological, economic, logistical, informational, cultural, and historical constraints. Individual contexts display their own unique combinations and relative significance of these constraints, and accordingly, targeted efforts, such as regulations and incentives, may help to overcome specific challenges.Conclusions: In a broader context, we propose that less emphases on strict applications of principles and on stand structures might provide additional flexibility and facilitate the adoption of alternative silvicultural regimes in a broader set of circumstances. At the same time, the acceptance of alternative silvicultural systems as the 'preferred or default mode of management' wil necessitate and benefit from the continued development of the scientific basis and valuation of a variety of ecosystem goods and services. This publication is aimed to further the discussion in this context.Klaus J Puettmann Scott McG Wilson Susan C Baker Pablo J Donoso Lars Dr?ssler Girma Amente Brian D Harvey Thomas Knoke Yuanchang Lu Susanna Nocentini Francis E Putz Toshiya Yoshida Jürgen Bauhus 2015Forest Ecosystems2015,2,2:4
12Highly sensitive solar-blind deep ultraviolet photodetector based on graphene/PtSe_(2)/β-Ga_(2)O_(3)2D/3D Schottky junction with ultrafast speed显示文摘There is an emerging need for high-sensitivity solar-blind deep ultraviolet(DUV)photodetectors with an ultra-fast response speed.Although nanoscale devices based on Ga_(2)O_(3)nanostructures have been developed,their practical applications are greatly limited by their slow response speed as well as low specific detectivity.Here,the successful fabrication of two-/three-dimensional(2D/3D)graphene(Gr)/PtSe2/β-Ga_(2)O_(3)Schottky junction devices for high-sensitivity solar-blind DUV photodetectors is demonstrated.Benefitting from the high-quality 2D/3D Schottky junction,the vertically stacked structure,and the superior-quality transparent graphene electrode for effective carrier collection,the photodetector is highly sensitive to DUV light illumination and achieves a high responsivity of 76.2 mA/W,a large on/off current ratio of~105,along with an ultra-high ultraviolet(UV)/visible rejection ratio of 1.8×104.More importantly,it has an ultra-fast response time of 12µs and a remarkable specific detectivity of~1013 Jones.Finally,an excellent DUV imaging capability has been identified based on the Gr/PtSe2/β-Ga_(2)O_(3)Schottky junction photodetector,demonstrating its great potential application in DUV imaging systems.Di Wu Zhihui Zhao Wei Lu Lukas Rogée Longhui Zeng Pei Lin Zhifeng Shi Yongtao Tian Xinjian Li Yuen Hong Tsang 2021Nano Research2021,14,6:4
13Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem显示文摘Water transport from roots to leaves through xylem is important for plant growth and development.Defects in water transport can cause drought stress,even when there is adequate water in the soil.Here,we identi-fied the maize(Zea mays)wilty5(wi5)mutant,which ex-hibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions.wilty5 seed-lings exhibited lower xylem conductivity and wilted more rapidly under drought,NaCl,and high temperature treat-ments than wild-type plants.Map-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from gly-cosyl dehydration family 10,which mainly functions in degrading and reorganizing cell wall xylan.Reverse-transcription polymerase chain reaction andβ-glucuronidase assays revealed that WI5 is highly expressed in stems,especially in internodes undergoing secondary wall as-sembly.RNA sequencing suggested that WI5 plays a unique role in internode growth.Immunohistochemistry and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall thickening.Lignin deposition and xylan content were markedly re-duced in wi5 compared to the wild-type plants.Our results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport,the drought stress response,and plant growth in maize.Xiaojiao Hu Yang Cui Xiaomin Lu Weibin Song Lei Lei Jinjie Zhu Jinsheng Lai Lizhu E Haiming Zhao 2020Journal of Integrative Plant Biology2020,62,10:3
14CCAR1 5' UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance显示文摘Gaopeng Li Xiaoli Wu Wenchang Qian Huayong Cai Xinbao Sun Weijie Zhang Sheng Tan Zhengsheng Wu Pengxu Qian Keshuo Ding Xuefei Lu Xiao Zhang Hong Yan Haifeng Song Shouhong Guang Qingfa Wu Peter E Lobie Ge Shan Tao Zhu 2016Cell Research2016,26,6:3
15Groundwater Systems and Eco-hydrological Features in the Main Karst Regions of China显示文摘Different karst water features and related water resources are present both in southern and northern China.There are over 3,358 well-developed karst ground river systems with total discharges in the dry season of about 420×108 m3 in the main karst regions in the southern part of China.Exploitation rates are only 8-15%.Over 100 larger karst spring systems in the main karst regions of northern China cover a catchment area from 500 km2 to over 4,000 km2,of which the average discharge appears to be from about 1 m3/s to 13 m3/s and the exploitation rates are 70-80%.Six aspects of the eco-hydrological features of some typical karst regions in China comprising water environment,ecological features,materials and structures between parent rock and soil,bio-geological processes and palynological studies(spore-pollen)are discussed.Qualitative evaluation of eco-geology and rocky desertification in the karst regions should be based on the main karst ecological conditions as well as artificial,i.e.,man-made impacts.LU Yaoru ZHANG Feng'e LIU Changli TONG Guobang ZHANG Yun 2006Acta Geologica Sinica(English Edition)2006,80,5:3
16Frequent amplification of a chr19q13.41 microRNA polycistron in aggressive primitive neuroectodermal brain tumors显示文摘Li M Lee KF Lu Y Clarke I Shih D Eberhart C Collins VP Van Meter T Picard D Zhou L Boutros PC Modena P Liang ML Scherer SW Bouffet E Rutka JT Pomeroy SL Lau CC Taylor MD Gajjar A Dirks PB Hawkins CE Huang A. 2009中国神经肿瘤杂志2009,7,4:3
17Multiple sgRNAs facilitate base editingmediated i-stop to induce complete and precise gene disruption显示文摘Dear Editor,Gene editing is a process to introduce desired changes into targeted loci of genomic DNA.Recently,type II clustered regularly in terspaced short palindromic repeats-associated Cas9 endonuclease(CRISPR/Cas9)system has been demonstrated as a versatile tool for engineering eukaryote genome(Hsu et al.,2014),such as in mice(Zuo et al.,2017).Kun Jia Zongyang Lu Fei Zhou Zhiqi Xiong Rui Zhang Zhiwei Liu Yu'e Ma Lei He Cong Li Zhen Zhu Dejing Pan Zhengxing Lian 2019Protein & Cell2019,10,11:3
18Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion显示文摘酸的微型环境,特别地酸察觉到的离子隧道 1a (ASIC1a ) ,被报导了象移植一样支持癌房间增长。在这研究,我们在胃的癌(GC ) 的移植和侵略上探索了 ASIC1a 的效果。ASIC1a 表示层次被 immunohistochemistry 从 16 个病人在配对的 GC 和邻近的正常纸巾检验。反向的抄写即时 PCR 和 immunoblotting 被进行与 ASIC1a 小发卡 RNA (shRNA ) 在 transfection 以后在 GC 房间线 AGS 估计 ASIC1a 表示层次。创伤愈合和 transwell 侵略试金被利用检测转移和侵略追随者 ASIC1a silencing。肿瘤形成被用来在 vivo 在 tumorigenicity 检测 ASIC1a 的角色。ASIC1a 表示水平在与非转移相比显示出手术后的转移的 GC 纸巾和非肿瘤纸巾是显著地更高的,这被发现。而且,有 shRNA 的 ASIC1a 的 silencing 显著地下面调整的 ASIC1a 表示和减少的 GC 房间移植和侵略。中等酸的细胞外的环境禁止了 GC 房间生存能力。而且, ASIC1a shRNA 在 vivo 引起了 tumorigenicity 的抑制。我们的学习是 attenuating 的第一份报告在 vitro 并且在由压制 ASIC1a 的 vivo 的 GC 的恶意的显型,并且建议一条新奇途径学习在 ASIC 和 GC 房间移植和侵略之间的关系。Xin Chen Xue Sun Zhe Wang Xiaojun Zhou Lu Xu Feng'e Li Xingding Zhang Ji'an Pan Lin Qi Haixin Qian Zhongqi Mao 2018Acta Biochimica et Biophysica Sinica2018,50,5:3
19Reconstructing the Primordial Spectrum of Fluctuations of the Universe from the Observed Nonlinear Clustering of Galaxies显示文摘Hamilton A J S Kumer P Lu E 1991ApJ1991,374,:3
20Tracking of CFSE-labeled endothelial progenitor cells in laser-injured mouse retina显示文摘背景 Endothelial 祖先房间(EPC ) 移植是为 ischemic retinopathy 的有希望的治疗学的策略。当前的学习试图为与 5-(and-6 ) 追踪 EPC 建立一个简单、可靠、荧光灯的标记的方法在伤害激光的老鼠 retina.Methods EPC 的 -carboxyfluorescein diacetate succinimidyl 酉旨(CFSE ) 从人的脐的绳索血 mononuclear 房间被孤立,栽培,并且用 CFSE 的各种各样的集中标记。基于荧光紧张和房间形态学,有在 37 点的 5 mol/L CFSE 的 15 分钟孵化作为最佳的标记条件被选择。幸存能力和标记 CFSE 的 EPC 的 apoptosis 率被染色的 Trypan 蓝色和 Annexin V/PI 染色试金分别地测量。荧光显微镜学被用来在扩大文化时期期间观察标签稳定性。标记的 EPC 被移植进网膜的激光光致疑结伤害的 pigmented 老鼠的玻璃的洞。Evans 蓝 angiography 和完全装的视网膜被检验追踪用 5 mol/L CFSE 介绍了标记的标记的 cells.Results EPC 一块强烈草地荧光和维持的正常形态学没有在在被标记 2 天以后的幸存能力或 apoptosis 率的重要变化, 1 和 4 个星期。荧光紧张逐渐地在 4 个星期的结束在房间减少了。视网膜的 Evans 蓝 angiography 清楚地显示了网膜的毛细作用网络,荧光漏在伤害激光的老鼠模型在 photocoagulated 点附近被观察。在标记的 EPC 的移植以后的一个星期,荧光灯房间在 photocoagulated 损害附近被识别。在移植以后的四个星期,荧光灯像试管的结构在网膜的脉管的 networks.Conclusion EPC 被观察能被 CFSE 在 vitro 标记并且在 vivo 监视了至少 4 个星期,并且参予受伤网膜的容器的修理。SHI Hui YANG Wei CUI Zhi-hua LU Cheng-wei LI Xiao-hong LIANG Ling-ling SONG E 2011Chinese Medical Journal2011,,5:3
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