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| 1 | Super-resolution dipole orientation mapping via polarization demodulation显示文摘Fluorescence polarization microscopy(FPM)aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy.Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume,with averaged fluorescence polarization collected from a group of dipoles with different orientations.Here,we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping(SDOM)method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area.We further apply this method to resolve structural details in both fixed and live cells.For the first time,we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation.Furthermore,we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling.The accuracy of the dipole orientation can be further mapped using the orientation uniform factor,which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area.Using the inherent feature of the orientation dipole,the SDOM technique,with its fast imaging speed(at sub-second scale),can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging. | Karl Zhanghao Long Chen Xu-San Yang Miao-Yan Wang Zhen-Li Jing Hong-Bin Han Michael Q Zhang Dayong Jin Jun-Tao Gao Peng Xi | 2016 | Light(Science & Applications)2016，5，1： | 11 |
| 2 | 饲料中添加蛋白酶对肉鸡性能、养分利用率、肠道形态和胴体品质的影响显示文摘试验在饲喂玉米、高粱为主的肉鸡日粮中补充大豆,并添加蛋白酶,研究蛋白酶对肉鸡性能、养分消化率、肠道形态和胴体品质的影响。试验选取1日龄AA肉仔鸡(43.8±1.31 g)256只,均为公鸡,随机分配到2×2试验设计方阵中,方阵包括正常组(玉米组和高粱组)和蛋白酶组(空白组和添加蛋白酶150 mg·kg^(-1)的试验组)。试验1~21 d为第一阶段,22~42 d为第二阶段。试验结果表明,第二阶段中,日粮中添加蛋白酶极显著提高肉鸡的平均日增重(ADG,P<0.01),在整个试验过程中显著提高(P<0.05)。在各个时期,正常组对肉鸡生产性能均无显著影响(P>0.05)。19~21 d时,添加蛋白酶可显著提高肉鸡的表观代谢能(AME),干物质(DM)、总能量(GE)和养分消化率(P<0.05)。此外,日粮以高粱为主时,AME、GE消化率有降低趋势(P=0.05),氮沉积率也有降低趋势(P=0.08)。40~42 d时,日粮中添加蛋白酶可显著提高AME,DM、GE和氮沉积率(P<0.05)。21 d时,日粮中添加蛋白酶极显著增加肠绒毛高度、肠绒毛与隐窝深度比值,极显著降低十二指肠、空肠、回肠的隐窝深度(P<0.01)。42 d时,日粮中添加蛋白酶显著增加空肠肠绒毛高度、绒毛高度与隐窝深度比值,显著降低隐窝深度(P<0.05)。日粮中添加蛋白酶显著增加胸肌重、24 h p H,显著降低胸肌的滴水损失(P<0.05)。试验结果表明,饲养阶段日粮中添加复合蛋白酶可提高肉鸡ADG;在第一阶段可改善AME、DM、GE和氮沉积率和肠道形态。补充蛋白酶可提高出栏重、胸肌重和24 h pH,降低胸肌的滴水损失。低单宁高粱日粮与玉米日粮相比,对肉鸡性能的影响相近。 | Xu X Wang H L Pan L Ma X K Tian Q Y Xu Y T Long S F Zhang Z H Piao X S | 2017 | 饲料博览2017，0，1： | 2 |
| 3 | The ASIC Design for Synthesizing Dual Channel Gauss White Noise显示文摘 | Cui W Han Y Q Long T | 2002 | Chinese Journal of Electronics2002，11，3： | 2 |
| 4 | Large-scale fabrication of single-Crystalline Mn3O4 nanowires via vapor phase growth显示文摘 | D P Yu Y Long | 2005 | Journal of Crystal Growth2005，279，： | 1 |
| 5 | Fabrication and Characterization of Single Electron Transistor on SOI显示文摘 | Wang Q Chen Y F Long S B | 2007 | Microelectronic Engineering2007，84，58： | 1 |
| 6 | Genetic polymorphism of Malus sieversii populations in Xinjiang, China 显示文摘 | Yan G R Long H Song W Q | 2008 | Genet Resour Crop Evol2008，，55： | 1 |
| 7 | Strong/weak summer monsoon activity over the South China Sea and atmospheric intraseasonal oscillation 显示文摘 | LI C Y LONG Z X ZHANG Q Y | 2001 | Adv Atmos Sci2001，18，： | 1 |
| 8 | Electrical conductivity of an individual pol yanili ne nanotube synthesized by a self-assembly method 显示文摘 | Long Y Z Zhang L Q | 2003 | Macromolecular Rapid C ommunications2003，24，16： | 1 |
| 9 | Application of the quadrilateral area coordinate method: A new element for Mindlin -Reissner plate 显示文摘 | Cen S Long Y Q Yao Z H Chiew S P | 2006 | International Journal for Numerical Methods in Engineering2006，66，1： | 1 |
| 10 | Isolation and characterization endophytic bacteria from Sedum alfredii Hance and their potential to promote phytoextraction of zinc polluted soil 显示文摘 | LONG X X CHEN X M CHEN Y G WONG J W WEI Z B WU Q T | 2010 | World Journal Microbiology Biotechnology2010，27，： | 1 |
| 11 | Human circulating microRNA-1 and microRNA-126 as potential novel indicators for acute myocardial infarction显示文摘 | Long G Wang F Duan Q | 2012 | Int J Biol Sci2012，8，6： | 1 |
| 12 | Functionalized N-doped interconnected carbon nanofibers as an anode material for sodium-ion storage with excellent performance显示文摘 | WANG Z LONG Q YUAN L | 2013 | Carbon2013，55，： | 1 |
| 13 | Seismic response analysis of structures with velocity-dependent dampers 显示文摘 | OU J P LONG X LI Q S | 2007 | Journal of ConstructionalSteel Research2007，63，： | 1 |
| 14 | Evaluation of double vital staining with lugolg iodine and methylene blue in diagnosing superficial esophageal lesions显示文摘 | Peng G Long Q Wu Y | 2011 | Scand J Gastroentero12011，46，4： | 1 |
| 15 | Current status of thalassemia in minority populations in Guangxi 显示文摘 | PAN H F LONG G F LI Q | 2007 | Clin Geuet2007，71，5： | 1 |
| 16 | Protective effects of bone marrow stromal cell transplantation in injured rodent brain: synthesis of neurotrophic factors 显示文摘 | Chen Q Long Y Yuan X | 2005 | J Neurosci Res2005，80，5： | 1 |
| 17 | Research on ZrsoAl~5-xNitoCu2sYx amorphous alloys prepared by mechanical alloying with commercial pure element powders显示文摘 | Long W Y Ouyang X Q Luo Z W | 2011 | Physica B2011，406，3： | 1 |
| 18 | Studies on comparison of esterase isozyme of different species of Trichogramma显示文摘 | CAO G L LU W Q LONG S | 1988 | Colloques de 1'' INRA1988，43，： | 1 |
| 19 | Resistive switching of Au-impanted ZrO2 film for nonvolatile memory appli cation 显示文摘 | Liu Q Guan W H Long S B | 2008 | J ApplPhys2008，104，11： | 1 |
| 20 | 显示文摘 | Wang Q Zheng H Long Y | 2011 | Carbon2011，49，9： | 1 |
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