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| 1 | 1990—2017年中国伤害负担:2017年全球疾病负担研究结果(摘译)显示文摘伤害是全球性的公共卫生挑战,涉及各年龄组和不同性别的人群。过去三十年来,中国经济快速发展,人口老龄化趋势加重,社会呈现出城镇化、机动化和老龄化等特征。随着人们生活环境和生活方式的改变,伤害已跃居人群致死原因第五位,仅排在恶性肿瘤、心脑血管疾病、呼吸系统疾病和心肌梗死之后。据估计,中国每年在伤害方面消耗的直接医疗费用约为650亿元人民币. | 林泽婷(译) 吕来文(译) 黄晓晴(译) 李丽萍(审校) Duan Leilei Ye Pengpeng Juanita A Haagsma Jin Ye Wang Yuan Er Yuliang Deng Xiao Gao Xin Ji Cuirong Wang Linhong Marlena S Bannick W Cli Mountjoy-Venning Caitlin N Hawley Zichen Liu Mari Smith Spencer L James Theo Vos Christopher J L Murray | 2020 | 伤害医学(电子版)2020，9，2： | 15 |
| 2 | Interleukin-1β and tumor necrosis factor-a increase stiffness and impair contractile function of articular chondrocytes显示文摘 | Cheng Chen JingXie Ravikumar Rajappa Linhong Deng Jeffrey Fredberg Liu Yang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，2： | 13 |
| 3 | Sulfoalkyl ether β-cyclodextrin derivatives synthesized by a single step method as pharmaceutical biomaterials显示文摘We report a simple 'one pot' single step method to synthesize sulfoalkyl ether β-cyclodextrin (β-CD) derivatives in aqueous solution, which avoids the limitations associated with commonly used organic synthesis of such derivatives including multi-step reactions, use of expensive, toxic, and often environmentally hazardous organic solvents and/or reagents. Using this method, we obtained sulfopropyl and sulfobutyl ethyl β-CDs. We characterized these two derivatives and evaluated their ability to enhance solubility of fluconazole, a commonly used antifungal drug with poor water solubility. Experimental results indicate that these derivatives are structurally analogous to some of the commercially available β-CD derivatives. More importantly, when they formed supramolecular inclusion complexes with fluconazole, they demonstrated similar, if not better, capacity to enhance fluconazole solubility compared with commercially available β-CD derivatives, suggesting that the simple 'one pot' single step synthesis may provide an effective alternative approach to produce large quantity of β-CD derivatives as pharmaceutical biomaterials for medicinal applications. | SONG AiJing WANG JianHua LIU ChunDong DENG LinHong | 2009 | Chinese Science Bulletin2009，54，18： | 4 |
| 4 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 5 | In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells显示文摘Cytoskeleton nanomechanics characterizes cancer cell's physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the Factin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G) of HepG2 cells was affected by TBMS I. G′ exhibited a typical power law with respect to the loading frequency (f), i.e. G^f . The magnitude of G′ and the value of exponent (α) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of Factin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates. | ZHAO HongBo WANG YaShu JIANG XueMei SHI XiaoHao ZHONG HongZhe WANG YaJie CHEN Jun DENG LinHong | 2013 | Chinese Science Bulletin2013，58，21： | 2 |
| 6 | Compressive Stress Enhances Invasive Phenotype of Cancer Cells via Piezo1 Activation显示文摘In this study,we hypothesized that Piezo 1 channels mediate the compression-enhanced invasive phenotype of cancer cells via a caveolae-dependent mechanism.To test this hypothesis,we examined in vitro cultured human breast cancer cells for their ability to invade and degrade extracellular matrix in the presence or absence of compressive stress,together with corresponding changes in Piezo1 as well as cytoskeletal remodeling and calcium signaling.Here we show that compressive stress enhanced invasion,matrix degradation,and invadopodia formation of breast cancer cells.We further identified Piezo1 as the putative mechanosensitive cellular component that transmits compression to induce calcium influx,which in turn triggers several downstream pathways.Interestingly,for the first time we observed inv-adopodia with matrix degradation ability on the apical side of the cells, similar to those commonly observed at the cell s ventral side.Furthermore,we demonstrate that Piezo1 and caveolae were both involved in mediating the compressive stress-induced cancer cell invasive phenotype as Piezo 1 and caveolae were often colocalized,and reduction of Cav-1 expression or disruption of caveolae with methyl-β-cyclodextrin led to not only reduced Piezo1 expression but also attenuation of the invasive phenotypes promoted by compressive stress.Taken together,we first observed that in breast cancer cells,simulating uncontrolled growth-induced compressive stress enhanced cancer cell invasion,matrix degradation,and invadopodia and stress fiber formation.Our study also confirmed that Piezo1 channels are highly expressed in breast cancer cells compared to normal breast cells,and is consistent with the data that compressive stress regulates cell migration of breast cancer cells but not normal breast cells.Additionally,we identified that Piezol mediated these processes and the invasive phenotypes also depended on the integrity of caveolae.These findings provide the first demonstration that compressive stress enhances matrix degradation by breast cancer cells and Piezo1 is an essential mechanosensor and transducer for such stress in breast cancer.Additionally,our data supports the model where caveolae might be the'mechanical force foci'which concentrates Piezol to facilitate force sensing and transduction in mammalian cells.Our work may have relevance to human tumors in vivo.As solid tumor experiences high compressive stress due to uncontrolled proliferation and confinement by the stiff extracellular matrix environment,this microenvironment facilitates compression-enhanced cell invasion.The identification of Piezo1’s crucial role in this process provides the first demonstration of the dependence of Piezo1 channels on the response of breast cancer cells to physiological compressive stress.The functional dependence of Piezo1 on caveolae further highlights the importance of membrane organization and composition on forcegated ion channels.Both of these findings underscore the cardinal role that Piezo1 channels play in regulating cell invasion and may inspire further development targeting Piezol as a potential cancer therapeutic target. | Mingzhi Luo Kenneth KYHo Zhaowen Tong Linhong Deng Allen PLiu | 2019 | 医用生物力学2019，34，A01： | 1 |
| 7 | Soluble ADAM33 determines mechanical behaviors of airway smooth muscle cells显示文摘Introduction A disintegrin and metalloproteinase 33(ADAM33)has been identified as a susceptibility gene for asthma[1],In particular,ADAM33 mRNA is highly expressed in airway smooth muscle cells(ASMCs)and the level of soluble ADAM33(sADAM33)is known to inversely correlate with lung function such as bronchial hyperresponsiveness(BHR)[2]that is ultimately determined by the mechanical behaviors of ASMCs.However,few have studied how sADAM33would affect ASMC mechanics.Here,we manipulated the expression of SADAM33 in | Yiyuan Duan Jiaoyue Long Feng Lin Jun Chen Rong Xu Xuemei Jiang Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 1 |
| 8 | Genetically Encoded FRET Biosensor Detects the Enzymatic Activity of Prostate-Specific Antigen显示文摘Prostate cancer is the most common cancer among men beyond 50 years old,and ranked the second in mortality.The level of Prostate-specific antigen(PSA)in serum has been a routine biomarker for clinical assessment of the cancer development,which is detected mostly by antibody-based immunoassays.The proteolytic activity of PSA also has important functions.Here a genetically encoded biosensor based on fluorescence resonance energy transfer(FRET)technology was developed to measure PSA activity.In vitro assay showed that the biosensor containing a substrate peptide‘RLSSYYSGAG’had 400%FRET change in response to 1µg/ml PSA within 90 min,and could detect PSA activity at 25 ng/ml.PSA didn’t show enzymatic activity toward the biosensor in serum solution,likely reflecting the existence of other inhibitory factors besides Zn^(2+).By expressing the biosensor on cell plasma membrane,the FRET responses were significant,but couldn’t distinguish well the cultured prostate cancer cells from non-prostate cancer cells under microscopy imaging,indicating insufficient speci-ficity to PSA.The biosensor with the previously known‘HSSKLQ’substrate showed little response to PSA in solution.In summary,we developed a genetically encoded FRET biosensor to detect PSA activity,which may serve as a useful tool for relevant applications,such as screening PSA activation substrates or inhibitors;the purified biosensor protein can also be an alternative choice for measuring PSA activity besides currently commercialized Mu-HSSKLQ-AMC substrate from chemical synthesis. | Hui Yao Liqun Wang Jia Guo Weimin Liu Jingjing Li Yingxiao Wang Linhong Deng Mingxing Ouyang | 2020 | Molecular & Cellular Biomechanics2020，17，3： | 0 |
| 9 | Chemical and physical regulations of airway smooth muscle cells and their implications to asthma pathogenesis显示文摘Introduction Although pathogenesis of asthma is still a major challenge in medical science,it is generally agreed that both structural and functional alterations of airway smooth muscle (ASM) are the common final pathway responsible for the excessive con- | Linhong Deng(Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University,Chongqing 400044,China.) | 2010 | 医用生物力学2010，25，S1： | 0 |
| 10 | Sanguinarine Decreases Cell Stiffness and Traction Force and Inhibits the Reactivity of Airway Smooth Muscle Cells in Culture显示文摘Airway hyperresponsiveness(AHR)is the cardinal character of asthma,which involves the biomechanical properties such as cell stiffness and traction force of airway smooth muscle cells(ASMCs).Therefore,these biomechanical properties comprise logical targets of therapy.β2-adrenergic agonist is currently the mainstream drug to target ASMCs in clinical practice for treating asthma.However,this drug is known for side effects such as desensitization and non-responsiveness in some patients.Therefore,it is desirable to search for new drug agents to be alternative ofβ2-adrenergic agonist.In this context,sanguinarine,a natural product derived from plants such as bloodroots,that has been reported to relax gut smooth muscle emerges as a potential candidate.So far,it is unknown whether sanguinarine can regulate the biomechanical properties of ASMCs and reactivity of ASMCs to irritants.Thus,we tested the hypothesis that sanguinarine reduce the contractile potentials of ASMCs in culture.To do so,the primary cultured rat ASMCs were first treated with different concentration of sanguinarine.Then,cell stiffness,traction force,fiber distribution,and calcium signaling of the ASMCs were evaluated by optical magnetic twisting cytometry,Fourier transform traction microscopy,atomic force microscopy,and Fluo-4/AM based fluorescence confocal scanning microscopy,respectively.The results indicated that sanguinarine(0.05 and 0.5μmol/L)significantly decreased cell stiffness and traction force,inhibited reactivity of ASMCs to histamine,and disrupted the fiber structures in ASMCs in dose-dependent manner.These findings establish that sanguinarine can indeed change the biomechanical properties of ASMCs and may be used to treat AHR in asthma. | Mingzhi Luo Kai Ni Peili Yu Yang Jin Lei Liu Jingjing Li Yan Pan Linhong Deng | 2019 | Molecular & Cellular Biomechanics2019，16，2： | 0 |
| 11 | IMAGE-BASED IN VIVO QUANTITATIVE ASSESSMENT OF HUMAN AIRWAY OPENING AND CONTR ACTILITY BY FIBER OPTICAL NA SOPH A RYNGOSCOPY IN HEALTHY AND ASTHMATIC SUBJECTS显示文摘Assessment of human airway humen opening is important in diagnosing and understanding the mechanisms of airway dysfunctions such as the excessive airway narrowing in asthma and chronic obstructive pulmonary disease(COPD).Although there are indirect methods to evaluate the airway calibre,direct in vivo measurement of the airway calibre has not been commonly available.With recent advent of the flexible fiber optical nasopharyngoscope with video recording it has become possible to directly visualize the passages of upper and lower airways.However,quan-titative analysis of the recorded video images has been technically challenging.Here,we describe an automatic image processing and analysis method that allows for batch analysis of the images recorded during the endoscopic procedure,thus facilitates image-based quantification of the airway opening.Video images of the airway lumen of volunteer subject were acquired using a fiber optical nasopharyngoscope,and subsequently processed using Gaussian smoothing filter,threshold segment ation,differentiation,and Canny image edge detection,respectively.Thus the area of the open airway lumen was identified and computed using.a predetermined converter of the image scale to true dimension of the imaged object.With this method we measured the opening/narrowing of the glottis during tidal breathing with or without making“Hee'sound or cough.We also used this met hod to measure the opening/narrowing of the primary bronchus of either healthy or asthmatic subjects in response to hist amine and/or albuterol treatment,which also provided an indicator of the airway contractility.Our results demonstrate that the image-based method accurately quantifed the area change waveform of either the glottis or the bronchus as observed by using the optical nasopharygoscope.Importantly,the opening/nar-rowing of the airway lumen generally correlated with the airAow and resistance of the airways,and could differentiate the level of airway contr actility between the healthy and asthmatic subjects.Thus,this quant itative assessment of airway opening may provide a useful tool to ssist clinical diagnosis of airway dysfunctions and understanding the mechanisms of associated pathophysiologies. | LINHONG DENG | 2013 | Journal of Innovative Optical Health Sciences2013，6，2： | 0 |
| 12 | OPTICALLY TRACKING THE MOTION OF MICROBEADS TO STUDY PHYSICAL BEHAVIORS OF THE LIVING CELL IN RESPONSE TO TRANSIENT STRETCH OR COMPRESSION显示文摘Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the cell.The former measures rheological properties of the cell such as cell stiffness,and the latter measures cell traction force dynamics.Here we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or compression.We demonstrate that,when subjected to a stretchunstretch manipulation,both the stiffness and traction force of adherent cells promptly reduced,and then gradually recover up to the level prior to the stretch.Immunofluorescent staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell mechanics.Interestingly,when subjected to compression,the cells did not show such particular behaviors.Taken together,we conclude that adherent cells are very sensitive to the transient stretch but not transient compression,and the stretch-induced cell response is due to the dynamics of actin polymerization. | LINHONG DENG XUEMEI JIANG CHENG CHEN AIJING SONG FENG LIN | 2011 | Journal of Innovative Optical Health Sciences2011，4，2： | 0 |
| 13 | Toward an optimized strategy of using various airway mucus clearance techniques to treat critically ill COVID-19 patients显示文摘Coronavirus disease 2019(COVID-19)caused by acute respiratory syndrome coronavirus 2(SARS-Cov-2)is still threatening the human life and society throughout the world.For those critically ill patients,mechanical ventilation(MV)is essential to provide life support during treatment.However,both the virus infection and MV disrupt the balance between secretion and elimination of airway mucus and lead to mucus accumulation in the lung.Postmortem examination verified that the lungs in patients died of COVID-19 are indeed filled with sticky mucus,suggesting a great need to improve airway mucus clearance in critically ill COVID-19 patients.Therefore,it may be helpful to comprehensively review the current understanding regarding the changes of biochemical and rheological features of airway mucus associated with the disease,as well as the physiological principles and algorithm to decide airway clearance techniques suitable for the critically ill COVID-19 patients.Based on these considerations,optimized strategies may be developed to eliminate the airway mucus accumulated in the airways of critically ill COVID-19 patients. | MINGZHI LUO KAI NI YAN SUN JIA GUO KANG WEN LINHONG DENG | 2022 | BIOCELL2022，46，4： | 0 |
| 14 | Sensing Traction Strain Induces Cell-Cell Distant Communications显示文摘Mechanobiology has been a highly recognized field in studying the importance of physical forces in physiologies at the molecular,cellular,tissue,organ and body-levels.Beside the intensive work focusing on the fine local biomechanical forces,the long-range force which can propagate through a relatively distant scale(in hundreds of micrometers and beyond)has been an intriguing topic with increasing attentions in recent years.The collective functions at cell population level often rely on cell-cell communications with or without direct contacts.Recent progresses including our own work indicate that the long-range biomechanical force propagating across scales far beyond single cell size may reserve the capability to trigger coordinative biological responses within cell population.Whether and how cells communicate mechanically in a distant manner remains largely to be explored.In respiratory system,the mechanical property of airway smooth muscle(ASM)is associated with asthma attack with prolonged contraction during airway hyper-responsiveness.In this work,we found that ASM cells rapidly self-assembled into a well-constructed network on 3D matrigel containing type I collagen(COL I),which required the collective functions and coordination of thousands of cells completed within 12-16 hours.Cells were assembled with aligned actin stress fibers and elongated nuclei.The assembling process relied on the long-range mechanical forces across the matrix to direct cell-cell distant interactions.We further found that single ASM cells could rapidly initiate multiple buds precisely pointing to neighboring cells in distance,which relied on cell traction force and force strain on the matrix.Beads tracking assay demonstrated the long-range transmission of cellular traction force to distant locations,and modeling of maximum strain distribution on matrix by finite element method predicted the consistency with cell directional protrusions and movements in experiments.Cells could sense each other in distance to move directionally on both non-fibrous matrigel and in much more efficient way when containing COL I.Cells recruited COL I from the hydrogel to build nearly identical COL I fibrous network to mechanically stabilize the cell network.Our results revealed that ASM cells can sense the traction strain transmitted through matrix to initiate distant communications and rapidly coordinate the network assembly at the population level through active cell-matrix interactions.As an interesting phenomenon,cells sound able to’make phone call’via the role of long-range mechanical force.In summary,this work demonstrated that long-range biomechanical force facilitates the collective functions of ASM cell population for network assembly.The cells reacted to traction strain on the matrix for distant communications,which resulted in directional budding and movement.Fibrous COL I had important roles in facilitating the efficiency of force transmission to induce the assembly and stabilizing the cell network.This work has helped advance the understanding of the feature andfunction of long-range biomechanical force at the cell population level.The observed high mechano-sensitivity of ASM cells might suggest a re-enforced feedback of enhanced contraction by excessive ASM under asthmatic condition. | Zhili Qian Yang Jin Bing Bu Yan Pan Linhong Deng Mingxing Ouyang | 2019 | 医用生物力学2019，34，A01： | 0 |
| 15 | The effect of inhibitor peptide for ADAM8 in attenuating ovalbumin-induced murine asthma显示文摘Targeted peptides have been identified as showing great promise for treatment of various diseases including asthma.Asthma is considered of difficuIt-to-treat due to its unclear etiology,thus usually requiring life-long treatment.Current strategies for asthma therapy are hampered with undesirable side effects,poor targeting and failure in modulating airway hyperresponsiveness,leading to pressing need of developing more targeted and effective therapeutic sites for asthma.Recently,a disintegrin and metalloproteinase 8(ADAM8)have been shown to over- | Jun Chen Xuemei Jiang Yiyuan Duan Jiaoyue Long Feng Lin Rong Xu Xiaojuan Zhang Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 0 |
| 16 | Mechanobiology of airway smooth muscle cells and its regulations in pathogenesis of asthma显示文摘Introduction Excessive narrowing of airways is the most important pathological feature of asthma,but its mechanism remains puzzling.One certain thing is that the contraction of airway smooth muscle(ASM)ultimately causes airway narrowing,thus both structural and functional alterations of airway smooth muscle(ASM)are thought as common final pathway responsible for the bronchial hyperresponsiveness(BHR),the hall mark of asthma.Many chemical and physical factors such as air pollutants,inflammatory agents,mechanical and geometrical properties of the microenvironment could influence structure and/or function of ASM cells.In addition,some re- | Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 0 |
| 17 | Passive stiffness in active-force depleted skinned airway smooth muscle显示文摘Smooth muscle cells can work properly over a large length range,drastic reorganization of the cytoskeletal structure has to occur,but the mechanism behind the process of cytoskeleton reorganization of smooth muscle is still poorly understood,especially with respect to unique problem of maintaining force generation and transmission within the confine of an ever-changing cytoskeleton in smooth muscle.Using a cytoskeletal preparation derived from skinned airway smooth muscle,we aimed at separating the contribution of actomyosin interaction and elucidating the cytoskeletal stiffness in smooth muscle.The results indicate that there is a Ca++de- | Bo Lan Peter D.Par Chun Y.Seow Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 0 |
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