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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level显示文摘 | Liu, LM Li, Y Zhu, Y Du, GC Chert, J | 2007 | 中国生物学文摘2007，21，10： | 12 |
| 3 | SALVIAFLASIDE AND SALVIAFLASIDE METHYL ESTER TWO NEW DEPSIDIC GLYCOSIDES FROM SALVIA FLAVA显示文摘SALVIAFLASIDEANDSALVIAFLASIDEMETHYLESTERTWONEWDEPSIDICGLYCOSIDESFROMSALVIAFLAVA￥LiMinAHAO;WenYiHE;XiaoTianLIANGandLianNiangLI... | Zhao, LM He, WY Liang, XT Li, LN | 1996 | Chinese Chemical Letters1996，7，5： | 3 |
| 4 | Effects of mesenchymal stem cells on differentiation, maturation, and function of human monocyte-derived dendritic cells显示文摘 | Zhang,W Ge,W Li,CH You,SG Liao,LM Han,Q Deng,WM Zhao,RCH | 2005 | 中国生物学文摘2005，19，3： | 3 |
| 5 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 6 | Densification of the Tungsten Heavy Alloys at 1473 K and Fabrication of W-Mo System FGM with Density Gradient显示文摘The tungsten heavy alloys with Ni-Al and Fe-Al additives were sintered by hot-press method at 1473 K for 1 h. It was found that the relativedensity of the W-Fe alloy could be increased evidently by addition of Al. However, the relative density of W- Ni-Al alloy with low content of Al was conversely decreased considerably compared with that of W-Ni alloy. The causes have been studied. The sinter ing experiments of Mo aloy and W-Mo alloys with 3 wt pct Fe-1.5 wt pct Al additives were also carried out under the same sintering condition of low temperature.Finally, W-Mo system FGM with density gradient was successfully fabricated.Its density changed gradually from 17.0x103 kg/m3 to 9.5x103 kg/m3 within the middfe 1.1 mm thickness | Xiong, HP Zhang, LM Li, JG Yuan, RZ | 1999 | Journal of Materials Science & Technology1999，15，3： | 2 |
| 7 | Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells显示文摘 | Tsai RJ Li LM Chen JK | 2000 | N Eng J Med2000，343，2： | 1 |
| 8 | Surgical treatment of 263cases tuberculous of the chestwall显示文摘 | WANG SX WANG SL LI LM | 1988 | Chin J Surg1988，26，3： | 1 |
| 9 | Communication via gapjunctions underlies early functional and beneficial interactionsbetween grafted neural stem cells and the host显示文摘 | Jaderstad JJaderstad LM Li J | | Proc NatlAcad Sci U S A0，107，： | 1 |
| 10 | Isoflurane post-conditioning protects primary cultures of cortical neurons against oxygen and glucose deprivation injury via upregulation of Slit2/Robol 显示文摘 | Zhao XC Zhang LM Li Q | 2013 | Brain Res2013，1537，： | 1 |
| 11 | A role for cofactor-cofactor and cofac-tor-histone interactions in targeting p300,SWI/SNF and mediator for transcription显示文摘 | Huang ZQ Li J Sachs LM | | 0，，： | 1 |
| 12 | A survey of core and support activities of communicable disease surveillance system operating-level CDCs in China 显示文摘 | Xiong WY Lv J Li LM | 2010 | BMC Public Health2010，10，： | 1 |
| 13 | Mechanisms of Rho kinase regulation of vascular reactivity following hemorrhagic shock in rats显示文摘 | Li T Liu LM Ming J | 2008 | Shock2008，29，1： | 1 |
| 14 | Comparative analysis of a large dataset indicates that internal transcribed spacer(ITS)should be incorporated into the core barcode for seed plants显示文摘 | Li DZ Gao LM Li H Wang H Ge X J Liu JQ Chen ZD Zhou SL Chen SL Yang JB | | 0，，： | 1 |
| 15 | MicroRNAs in drug - inducedliver injury显示文摘 | LI LM WANG D ZEN K | 2014 | J Clin Transl Hepatol2014，2，3： | 1 |
| 16 | Human papillomavirus testing and cervical cytology in primary screening for cervical cancer among women in rural China: Compariaon of sensitivity, specificity, and frcquency of referral显示文摘 | Moy LM Zhao FH Li LY | 2010 | Int J Cancer2010，127，3： | 1 |
| 17 | Dimensionality reduction of hyperspectral data using discrete wavelet transform feature extraction显示文摘 | Bruce LM Koger CH Li J | 2002 | IEEE Trans Geosci Remote Sens2002，，40： | 1 |
| 18 | Prognostic value of proton mag- netic resonance spectroscopic imaging for surgical outcome in patients with intractable temporal lobe epilepsy and bilateral hippocampal atro- phy显示文摘 | Li LM Cendes F Antol SB | 2003 | Ann Neurol2003，47，： | 1 |
| 19 | Surgical outcome in patients with epilepsy and dual pathology显示文摘 | Li LM Cendes F Andermann F | 1999 | Brain1999，122，5： | 1 |
| 20 | Surgical outcome inpatients with epilepsy and dual pathology显示文摘 | Li LM Cendes F Andermann F | 1999 | Brain1999，122，： | 1 |
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