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| 1 | The human papiliomavirus type 16 E2 transcription factor binds with low cooperativity to two nanking sites and represses the E6 promoter through displacement of Sp1 and TFIID显示文摘 | Tan SH Leong LE Walker PA | 1994 | J Viro1994，68，10： | 1 |
| 2 | Controlled gene delivery by DNA - gelatin nanospheres 显示文摘 | Truong - Le VL August JT Leong KW | 1998 | Hum Gene Ther1998，9，12： | 1 |
| 3 | Efficient and rapid affinity purification of proteins using recombinant fusion proteases 显示文摘 | Walker PA Leong LE Ng PW | 1994 | Biotechnol1994，12，6： | 1 |
| 4 | Fouling control mechanism by suspended biofilm carriers addition in submerged ceramic membrane bioreactors显示文摘 | Le Jin Say Leong Ong How Yong Ng | 2013 | Journal of Membrane Science2013，，： | 1 |
| 5 | Efficient and rapid purification of proteins using recombinant fusion proteases 显示文摘 | Walker PA Leong LE Ng PW | 1994 | Biotechnology1994，12，6： | 1 |
| 6 | Coculture ofmesenchymal stem cells and respiratory epithelial cells to engineer a human composite respiratory mucosa显示文摘 | Le Visage C Dunham B Flint P Leong KW | 2004 | Tissue Eng2004，10，： | 1 |
| 7 | Dual-polarized slot-coupled planar antenna with wide bandwidth显示文摘 | Shi - chang Gao Le wei Li Mook - seng Leong | 2003 | IEEE Transaction on antenna and propogation2003，51，3： | 1 |
| 8 | The human papillomavirus type 16 E2 transcription factor binds with low cooperativity to two flanking sites and represses the E6 promoter through displacement of Spl and TFIID显示文摘 | TAN SH LEONG LE WALKER PA | 1994 | J Virol1994，68，10： | 1 |
| 9 | The human papillomavirus type 16 E2 transcription factor binds with low cooperativity to two flanking sites and represses the E6 promoter through displacement of Sp1 and TFIID显示文摘 | Tan SH Leong LE Walker PA | 1994 | J Virol1994，68，： | 1 |
| 10 | The use of recombinant fusion proteases in the affinity purification of recombinant proteins 显示文摘 | Leong LE | 1999 | Mol Biotechnol1999，12，3： | 1 |
| 11 | Small Intestinal Submucosa as a Potential Bioscaffold for Intervertebral Disc Regeneration显示文摘 | Catherine Le Visage Shu-Hua Yang Leena Kadakia Ann N. Sieber John P. Kostuik Kam W. Leong | 2006 | Spine2006，，21： | 1 |
| 12 | MFSD7c functions as a transporter of choline at the blood–brain barrier显示文摘Mutations in the orphan transporter MFSD7c(also known as Flvcr2),are linked to Fowler syndrome.Here,we used Mfsd7c knockout(Mfsd7c–/–)mice and cell-based assays to reveal that MFSD7c is a choline transporter at the blood–brain barrier(BBB).We performed comprehensive metabolomics analysis and detected differential changes of metabolites in the brains and livers of Mfsd7c–/–embryos.Particularly,we found that choline-related metabolites were altered in the brains but not in the livers of Mfsd7c–/–embryos.Thus,we hypothesized that MFSD7c regulates the level of choline in the brain.Indeed,expression of human MFSD7c in cells significantly increased choline uptake.Interestingly,we showed that choline uptake by MFSD7c is greatly increased by choline-metabolizing enzymes,leading us to demonstrate that MFSD7c is a facilitative transporter of choline.Furthermore,single-cell patch clamp analysis showed that the import of choline by MFSD7c is electrogenic.Choline transport function of MFSD7c was shown to be conserved in vertebrates,but not in yeasts.We demonstrated that human MFSD7c is a functional ortholog of HNM1,the yeast choline importer.We also showed that several missense mutations identified in patients exhibiting Fowler syndrome had abolished or reduced choline transport activity.Mice lacking Mfsd7c in endothelial cells of the central nervous system suppressed the import of exogenous choline from blood but unexpectedly had increased choline levels in the brain.Stable-isotope tracing study revealed that MFSD7c was required for exporting choline derived from lysophosphatidylcholine in the brain.Collectively,our work identifies MFSD7c as a choline exporter at the BBB and provides a foundation for future work to reveal the disease mechanisms of Fowler syndrome. | Xuan Thi Anh Nguyen Thanh Nha Uyen Le Toan Q.Nguyen Hoa Thi Thuy Ha Anna Artati Nancy C.P.Leong Dat T.Nguyen Pei Yen Lim Adelia Vicanatalita Susanto Qianhui Huang Ling Fam Lo Ngah Leong Isabelle Bonne Angela Lee Jorge L.Granadillo Catherine Gooch Dejie Yu Hua Huang Tuck Wah Soong Matthew Wook Chang Markus R.Wenk Jerzy Adamski Amaury Cazenave-Gassiot Long N.Nguyen | 2024 | Cell Research2024，34，3： | 0 |
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