| 1 | Data learning and expert judgment in a Bayesian belief network for aiding human reliability assessment in offshore decommissioning risk assessment显示文摘Decommissioning of offshore facilities involve changing risk profiles at different decommissioning phases.Bayesian Belief Networks(BBN)are used as part of the proposed risk assessment method to capture the multiple interactions of a decommissioning activity.The BBN is structured from the data learning of an accident database and a modification of the BBN nodes to incorporate human reliability and barrier performance modelling.The analysis covers one case study of one area of decommissioning operations by extrapolating well workover data to well plugging and abandonment.Initial analysis from well workover data,of a 5-node BBN provided insights on two different levels of severity of an accident,the’Accident’and’Incident’level,and on its respective profiles of the initiating events and the investigation-reported human causes.The initial results demonstrate that the data learnt from the database can be used to structure the BBN,give insights on how human reliability pertaining to well activities can be modelled,and that the relative frequencies from the count analysis can act as initial data input for the proposed nodes.It is also proposed that the integrated treatment of various sources of information(database and expert judgement)through a BBN model can support the risk assessment of a dynamic situation such as offshore decommissioning. | Mei Ling Fam Dimitrios Konovessis XuHong He Lin Seng Ong | 2021 | Journal of Ocean Engineering and Science2021，6，2： | 1 |
| 2 | MFSD7c functions as a transporter of choline at the blood–brain barrier显示文摘Mutations in the orphan transporter MFSD7c(also known as Flvcr2),are linked to Fowler syndrome.Here,we used Mfsd7c knockout(Mfsd7c–/–)mice and cell-based assays to reveal that MFSD7c is a choline transporter at the blood–brain barrier(BBB).We performed comprehensive metabolomics analysis and detected differential changes of metabolites in the brains and livers of Mfsd7c–/–embryos.Particularly,we found that choline-related metabolites were altered in the brains but not in the livers of Mfsd7c–/–embryos.Thus,we hypothesized that MFSD7c regulates the level of choline in the brain.Indeed,expression of human MFSD7c in cells significantly increased choline uptake.Interestingly,we showed that choline uptake by MFSD7c is greatly increased by choline-metabolizing enzymes,leading us to demonstrate that MFSD7c is a facilitative transporter of choline.Furthermore,single-cell patch clamp analysis showed that the import of choline by MFSD7c is electrogenic.Choline transport function of MFSD7c was shown to be conserved in vertebrates,but not in yeasts.We demonstrated that human MFSD7c is a functional ortholog of HNM1,the yeast choline importer.We also showed that several missense mutations identified in patients exhibiting Fowler syndrome had abolished or reduced choline transport activity.Mice lacking Mfsd7c in endothelial cells of the central nervous system suppressed the import of exogenous choline from blood but unexpectedly had increased choline levels in the brain.Stable-isotope tracing study revealed that MFSD7c was required for exporting choline derived from lysophosphatidylcholine in the brain.Collectively,our work identifies MFSD7c as a choline exporter at the BBB and provides a foundation for future work to reveal the disease mechanisms of Fowler syndrome. | Xuan Thi Anh Nguyen Thanh Nha Uyen Le Toan Q.Nguyen Hoa Thi Thuy Ha Anna Artati Nancy C.P.Leong Dat T.Nguyen Pei Yen Lim Adelia Vicanatalita Susanto Qianhui Huang Ling Fam Lo Ngah Leong Isabelle Bonne Angela Lee Jorge L.Granadillo Catherine Gooch Dejie Yu Hua Huang Tuck Wah Soong Matthew Wook Chang Markus R.Wenk Jerzy Adamski Amaury Cazenave-Gassiot Long N.Nguyen | 2024 | Cell Research2024，34，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员