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| 1 | Temperature-dependent THz vibrational spectra of clenbuterol hydrochloride显示文摘Using the high-resolution Terahertz Time-domain spectroscopy (THz-TDS) and the standard sample pellet technique, the far-infrared vibrational spectra of clenbuterol hydrochloride (CH), a 2 -adrenergic agonist for decreasing fat deposition and enhancing protein accretion, were measured in temperature range of 77-295 K. Between 0.2 and 3.6 THz (6.6-120.0 cm-1 ), seven highly resolved spectral features, strong line-narrowing and a frequency blue-shift were observed with cooling. However, ractopamine hydrochloride, with some structural and pharmacological similarities to clenbuterol hydrochloride, showed no spectral features, indicating high sensitivity and strong specificity of THz-TDS. These results could be used for the rapid and nondestructive CH residual detection in food safety control. | YANG YuPing LEI XiangYun YUE Ai ZHANG ZhenWei | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，4： | 9 |
| 2 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 3 | Research of an SHPB Device in Two-by-Two Form for Impact Experiments of Concrete-Like Heterogeneous Materials显示文摘A split Hopkinson pressure bar(SHPB)device in two-by-two form,including the bar bundle form and the single cylindrical bar form,was designed in response to the demand for the dynamic mechanical experiments for brittle materials such as concrete,rock,etc.The stress waveforms generated through a projectile impacting two different types of incident bars have been studied based on the one-dimensional stress wave theory and numerical simulation method.At last,based on the established two types of mesoscale concrete models with random convex polyhedral aggregates,we performed comparison analysis of SHPB numerical simulations for concrete materials with bar bundle and single cylindrical bar separately,so as to provide technical support for the manufacture and development of this experimental device.The results showed that the introduced two-by-two form SHPB device expanded the scope of practical application,and the wave dispersion effect existing in a large-diameter bar can be evidently reduced when we employed the bar bundle form,indicating its applicability to the dynamic mechanical experiments of concrete-like heterogeneous materials. | Ruiqi Guo Huiqi Ren Lei Zhang Zhilin Long Xiangyun Wu Hailu Wang | 2021 | Acta Mechanica Solida Sinica2021，34，4： | 2 |
| 4 | Influence of modulation ratio on structure and properties of nanoscale ZrB_2/ZrAlN multilayered coatings显示文摘ZrB2/ZrAlN multilayered coatings with various modulation ratios(t ZrB2:t ZrAlN)and constant modulation periods were prepared by magnetron sputtering system at room temperature.SEM,XRD,surface profiler and nano-indenter were employed to investigate the influences of tZ rB 2:tZrAlNon the microstructure and mechanical properties of the coatings.Sharp interfaces and nanoscale multilayered modulation were confirmed by SEM.The multilayer with modulation period of 40 nm and ratio of 3:1 displayed the highest hardness(36.4 GPa)and critical fracture load(76.477 mN)with lower stress.The polycrystalline structure and multilayered modulate structure were directly responsible for the enhanced mechanical properties. | LI DeJun 1,KANG YuanBin 1,DONG Lei 1,DENG XiangYun 1,LIU Hao 2 &SUN XueLiang 2 1College of Physics and Electronic Information Science,Tianjin Normal University,Tianjin 300387,China 2 Department of Mechanical&Materials Engineering,University of Western Ontario,London ON,Canada | 2010 | Science China(Technological Sciences)2010，53，3： | 1 |
| 5 | Ubiquitin-specific protease 47 regulates intestinal inflammation through deubiquitination of TRAF6 in epithelial cells显示文摘Deubiquitinates(DUBs) alter the stabilities, localizations or activities of substrates by removing their ubiquitin conjugates,which are closely related to the development of inflammatory response. Here, we show that ubiquitin-specific protease 47(USP47) prevents inflammation development in inflammatory bowel disease(IBD). Compared with wild-type mice, Usp47 knockout mice are more susceptible to dextran sodium sulfate(DSS)-induced acute and chronic colitis with higher inflammatory cytokines expression and severe intestinal tissue damage. Chimeric mouse experiments suggest that non-hematopoietic cells mainly contribute to the phenotype. And, DSS-induced colitis of the Usp47 knockout mice depends on commensal bacteria.Mechanistically, down-regulation of USP47 aggravates the activation of NF-κB signaling pathway by increasing the K63-linked poly-ubiquitination of tumor necrosis factor receptor-associated factor 6(TRAF6) in intestinal epithelial cells. Furthermore, the expression of USP47, negatively correlated with the degree of inflammation, is lower at colonic inflammatory lesions than that non-inflammatory sites from the intestine from ulcerative colitis(UC) and Crohn's disease(CD) patients. These data, taken together, indicate that USP47 regulates intestinal inflammation through de-ubiquitination of K63-linked poly-ubiquitination TRAF6 in intestinal epithelial cells. | Hu Lei Li Yang Hanzhang Xu Zhengting Wang Xiangyun Li Meng Liu Yingli Wu | 2022 | Science China(Life Sciences)2022，65，8： | 1 |
| 6 | Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons显示文摘 | Lei Zhang Jiu-Chao Yin Hana Yeh Ning-Xin Ma Grace Lee Xiangyun Amy Chen Yanming Wang Li Lin Li Chen Peng Jin Gang-Yi Wu Gong Chen | 2015 | Cell Stem Cell2015，，： | 1 |
| 7 | USP7 deubiquitinates and stabilizes NOTCH1 in T-cell acute lymphoblastic leukemia显示文摘T-cell acute lymphoblastic leukemia(T-ALL)is a highly aggressive leukemia that is primarily caused by aberrant activation of the NOTCH1 signaling pathway.Recent studies have revealed that posttranslational modifications,such as ubiquitination,regulate NOTCH1 stability,activity,and localization.However,the specific deubiquitinase that affects NOTCH1 protein stability remains unestablished.Here,we report that ubiquitin-specific protease 7(USP7)can stabilize NOTCH1.USP7 deubiquitinated NOTCH1 in vivo and in vitro,whereas knockdown of USP7 increased the ubiquitination of NOTCH1.USP7 interacted with NOTCH1 protein in T-ALL cells,and the MATH and UBL domains of USP7 were responsible for this interaction.Depletion of USP7 significantly suppressed the proliferation of T-ALL cells in vitro and in vivo,accompanied by downregulation of the NOTCH1 protein level.Similarly,pharmacologic inhibition of USP7 led to apoptosis of T-ALL cells.More importantly,we found that USP7 was significantly upregulated in human T-ALL cell lines and patient samples,and a USP7 inhibitor exhibited cell cytotoxicity toward primary T-ALL cells,indicating the clinical relevance of these findings.Overall,our results demonstrate that USP7 is a novel deubiquitinase that stabilizes NOTCH1.Therefore,USP7 may be a promising therapeutic target in the currently incurable T-ALL. | Huizhuang Shan Xiangyun Li Xinhua Xiao Yuting Dai Jinyan Huang Junjun Song Meng Liu Li Yang Hu Lei Yin Tong Li Zhou Hanzhang Xu Yingli Wu | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 0 |
| 8 | Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy显示文摘The slow regenerating rate and misdirected axonal growth are primary concerns that disturb the curative outcome of peripheral nerve repair.Biophysical intervention through nerve scaffolds can provide efficient,tunable and sustainable guidance for nerve regrowth.Herein,we fabricate the reduced graphene oxide(rGO)/polycaprolactone(PCL)scaffold characterized with anisotropic microfibers and oriented nanogrooves by electrospinning technique.Adipose-derived stem cells(ADSCs)are seeded on the scaffolds in vitro and the viability,neural differentiation efficiency and neurotrophic potential are investigated.RGO/PCL conduits reprogram the phenotype of seeded cells and efficiently repair 15 mm sciatic nerve defect in rats.In summary,biophysical cues on nerve scaffolds are key determinants to stem cell phenotype,and ADSC-seeded rGO/PCL oriented scaffolds are promising,controllable and sustainable approaches to enable peripheral nerve regeneration. | Xiangyun Yao Lei Zhan Zhiwen Yan Juehong Li Lingchi Kong Xu Wang Huimin Xiao Huiquan Jiang Chen Huang Yuanming Ouyang Yun Qian Cunyi Fan | 2023 | Bioactive Materials2023，，2： | 0 |
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