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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | A Three-Dimensional Range-Free Localization Algorithm Based on Mobile Beacons for Wireless Sensor Networks显示文摘In wireless sensor networks (WSNs) the position information of individual nodes is a matter of vital importance because allows the implementation of necessary network functions such as routing, querying and other applications. The objective of this paper is to propose an algorithm of three-dimensional distributed range-free localization for WSNs, using a mobile beacon (MB) equipped with a rotary and tilting directional antenna. This algorithm, denominated as the three-dimensional azimuthally defined area localization algorithm (3D- ADAL), is executed in each sensor node and is based only on the analysis of the information received from the MB, therefore is energy efficient and contributes to extend the lifetime of the sensor network. Additionally the proposed algorithm has the advantage of being simple and economical. The simulation results show that the proposed algorithm is a practical, effective and accurate method for a three-dimensional location of sensor nodes in a WSN. | GUERRERO E ALVAREZ J RIVERO L | 2010 | Computer Aided Drafting,Design and Manufacturing2010，20，1： | 3 |
| 3 | PPARγ promptes monocyte/macrophage differentiation and uptake of oxidized LDL显示文摘 | Tontonoz P Nagy L Alvarez JGA | 1998 | Cell1998，93，2： | 2 |
| 4 | Nutrient and heavy metal concentration and distribution in corn, soybean and wheat as affected by different tillage systems in the Argentine Pampas显示文摘 | Raúl S Lavado Claudia A Porcelli Roberto Alvarez | 2001 | Soil & Tillage Research2001，，1： | 2 |
| 5 | Oxidized LDL regulates macrophage gene expression through ligand activation of PPAR gamma显示文摘 | Nagy L Tontonoz P Alvarez JG | 1998 | Cell1998，93，2： | 2 |
| 6 | Stereotactic biopsy for brainstem tumors in pediatric patients 显示文摘 | PEREZ GOMEZ J L RODRIGUEZ ALVAREZ C A MARHX BRACHO A | 2010 | Childs Nerv Syst2010，26，1： | 1 |
| 7 | Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection显示文摘 | Lopez F L Domentch N Alvarez B | 1999 | Virus Res1999，64，1： | 1 |
| 8 | Acetaldehyde increases the activity and gene expression of urokinase type plasminogen activator in a hepatic stellate cell line显示文摘 | Rodrīguez-Fragoso L Alvarez R Reyes-Esparza JA Garcés ME | 1999 | Toxicology1999，137，： | 1 |
| 9 | Lesion of the subthalamic nucleus in Parkinson's disease:long term follow-up显示文摘 | Alvarez Gonzalez L Macias R Guridi J | 1999 | Ann Neurol1999，46，： | 1 |
| 10 | Predictors of outcomes of percuta- neous vertebroplasty for osteoporotic vertebral fractures显示文摘 | Alvarez L Perez-Higueras A Granizo J J | 2005 | Spine2005，30，1： | 1 |
| 11 | lmage selective smoothing and edge detection by nonlinear diffusion Ⅱ显示文摘 | Alvarez L Lions PL Morel JM | 1992 | SIAM J Num Anal1992，29，3： | 1 |
| 12 | Adaptive emergency braking control with underestimation of friction co- efficient显示文摘 | Yi J Alvarez L Horowitz R | 2002 | IEEE Transactions on Control Systems Technology2002，10，3： | 1 |
| 13 | Retent ion of heavymetal ions in bentonites from Grau Reg ion (North crn Peru) 显示文摘 | AYALA J VEGA J L ALVAREZ R | 2008 | Environmental Geology2008，53，6： | 1 |
| 14 | Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal 显示文摘 | Aldea C Alvarez C P Folgueira L | 2002 | J Clin Microbiol2002，40，3： | 1 |
| 15 | Mucosal reactive oxygen species decrease virulence by disrupting Campylobacter jejuni phosphotyrosine signaling显示文摘 | CORCIONIVOSCHI N ALVAREZ L A SHARP T H | 2012 | Cell Host Microbe2012，12，1： | 1 |
| 16 | Tungsten and tungsten carbide supported on activated carbon: Surface structure and performance for ethylene hydrogenation显示文摘 | MORENO C C ALVAREZ M A CARRASCO M F FIERRO J L | 2001 | Langmuir2001，17，5： | 1 |
| 17 | Price Setting in The Eum Area Some Stylized Facts from Individual Consumer Price Data 显示文摘 | Dhyne E Alvarez L | 2005 | ECB Working Paper Seires2005，524，： | 1 |
| 18 | Changes in polyamines, proline and ethylene in sunflower calluses treated with NaCl 显示文摘 | Alvarez I Tomaro M L Benavides M P | 2003 | Plant Cell Tissue Organ Cult2003，74，： | 1 |
| 19 | 显示文摘 | Alvarez L W Arguelles -Monal W | 1992 | J Appl Polymer Sci1992，46，5： | 1 |
| 20 | Kidney transplant patients with long-term graft survival have altered expression of molecules associated with T-eellaetivation 显示文摘 | Alvarez CM Paris SC Arango L | 2004 | Transplantation2004，78，10： | 1 |
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