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| 1 | Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars显示文摘D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in nature.Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase.In this study,the crystal structure of the C.cellulolyticum DPEase was determined.The enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active site.Additional crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also determined.From the complex structures of C.cellulolyticum DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues.Accordingly,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here.These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun | 2012 | Protein & Cell2012，3，2： | 6 |
| 2 | Pandemic of the aging society—sporadic cerebral small vessel disease显示文摘Age-related sporadic cerebral small vessel disease(CSVD)has gained increasing attention over the past decades because of its increasing prevalence associated with an aging population.The widespread application of and advances in brain magnetic resonance imaging in recent decades have significantly increased researchers’understanding in the in vivo evolution of CSVD,its impact upon the brain,its risk factors,and the mechanisms that explain the various clinical manifestation associated with sporadic CSVD.In this review,we aimed to provide an update on the pathophysiology,risk factors,biomarkers,and the determinants and spectrum of the clinical manifestation of sporadic CSVD. | Alexander Yuk Lun Lau Bonaventure Yiu Ming Ip Ho Ko Bonnie Yin Ka Lam Lin Shi Karen Ka Yan Ma Lisa Wing Chi Au Yannie Oi Yan Soo Thomas Wai Hong Leung Adrian Wong Vincent Chung Tong Mok | 2021 | Chinese Medical Journal2021，，2： | 3 |
| 3 | Leptin Signaling Protects NK Cells from Apoptosis During Development in Mouse Bone Marrow显示文摘增加的证据在有免疫力的反应显示 leptin 的一个角色,但是发信号的 leptin 是否涉及在骨头髓(BM ) 调整 NK 房间开发,仍然保持大部分不清楚。在这研究,我们在在 prediabetic 的缺乏的 db/db 老鼠上演的 leptin 受体的 BM 描绘了 NK 房间区别和成熟。尽管 BM 细胞质类似于控制值, NK 房间的全部的数字严重地在变异的鼠标被减少。db/db BM 房间的流动 cytometric 分析揭示了在区别的各种各样的阶段开发 NK 房间的显著地减少的频率。显著地显示的 BM db/db NK 房间增加了 apoptosis,但是维持了正常房间骑车地位和 proliferative 能力。而且, recombinant leptin 能显著地在文化从野类型的老鼠提高 NK 房间的幸存。NK 房间上的进一步的检查功能的活动证明 db/db NK 房间与显著地增加的 IL-10 展出了正常内在的 cytotoxicity 生产。一起拿,我们的调查结果建议发信号的 leptin 经由在老鼠 BM 提高不成熟的 NK 房间的幸存调整 NK 房间开发。 | Cherry Kam Chun Lo Queenie Lai Kwan Lam Min Yang King-Hung Ko Lingyun Sun Rui Ma Shengjun Wang Huaxi Xu Sidney Tam Chang-You Wu Bo-Jiang Zheng Liwei Lu | 2009 | Cellular & Molecular Immunology2009，6，5： | 2 |
| 4 | Updated population-based review of carcinoid tumors 显示文摘 | Maggard MA O' Connell JB Ko CY | 2004 | Ann Surg2004，240，1： | 1 |
| 5 | Sak/PLK4 and mitotic fi- delity显示文摘 | Swallow CJ Ko MA Siddiqui NU | 2005 | Oncogene2005，24，2： | 1 |
| 6 | Vision guided navigation for nonholonomic mobile robot显示文摘 | Koecká J Sastry S | 1999 | IEEE Transactions on Robotics and Automation1999，15，3： | 1 |
| 7 | Outc-omes from out-of-hospital cardiac arrest in metropolitan taipei:does an advanced life support service make a difference显示文摘 | Ma MH Ching WC Ko PC | 2007 | J Resuscitation2007，74，2： | 1 |
| 8 | Colon cancer survival rates with the new American Joint Committee on Cancer sixth editionstaging显示文摘 | O'ConnellJB Maggard MA Ko CY | 2004 | J Natl Cancer Inst2004，96，19： | 1 |
| 9 | Endothelial lipase modulates HDL but has no effect on atherosclerosis development in apoE-/-and LDLR-/-mice显示文摘 | Ko KW Paul A Ma K | | 0，，12： | 1 |
| 10 | Updated population -based re- view of careinoid tumors显示文摘 | Maggard MA O'Connell JB Ko CY | 2004 | Ann Surg2004，240，1： | 1 |
| 11 | Prognostic factors affecting the clinical outcome of adenoid cystic carcinoma of the head and neck 显示文摘 | Ko YH Lee MA Hong YS | 2007 | Jpn J Clin Oncol2007，37，11： | 1 |
| 12 | Updated population-based review of carcinoid tumors显示文摘 | Maggard MA O Connell JB Ko CY | 2004 | Ann Surg2004，240，1： | 1 |
| 13 | Threee different binding sites of Cks1 are required for p27-ubiquitin ligation显示文摘 | Sitry D Seelger MA Ko TK | 2002 | J Biol Chem2002，277，42： | 1 |
| 14 | Updated population - based review of carcinoid tumors 显示文摘 | Maggard MA O'Connell JB Ko CY | 2004 | Ann Surg2004，240，1： | 1 |
| 15 | Endothelial lipase modu- lates HDL but has no effect on atherosclerosis development in apoE^-/- and LDLR^-/- mice显示文摘 | Ko KW Paul A Ma K | 2005 | J Lipid Res2005，46，： | 1 |
| 16 | Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging显示文摘 | OConnell JB Maggard MA Ko CY | 2004 | J Natl Cancer Inst2004，96，14： | 1 |
| 17 | Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging显示文摘 | O'Connell JB Maggard MA Ko CY | 2004 | J Natl Cancer Inst2004，96，19： | 1 |
| 18 | Colon cancer survival rates with the New American Joint Committee on Cancer sixth edition staging显示文摘 | O'Connell JB Maggard MA Ko CY | 2004 | J Natl Cancer Inst2004，96，19： | 1 |
| 19 | Pilot Results of the Early Detection by Ultrasound of Carotid Artery Intima-M edia Thickness Evaluation(EDUCATE)study显示文摘 | Ak osah KO Ma Hugh VL Barnhart SI | 2007 | AmJ Hypertens2007，20，： | 1 |
| 20 | Updated populaion based review of carcinoid tumors显示文摘 | Maggard MA OConnell JB Ko CY | 2004 | Ann Surg2004，240，1： | 1 |
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