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| 1 | Loss of Aβ-nerve endings associated with the Merkel cell-neurite complex in the lesional oral mucosa epithelium of lichen planus and hyperkeratosis显示文摘The Merkel cell-neurite complex initiates the perception of touch and mediates Ab slowly adapting type I responses.Lichen planus is a chronic inflammatory autoimmune disease with T-cell-mediated inflammation,whereas hyperkeratosis is characterized with or without epithelial dysplasia in the oral mucosa.To determine the effects of lichen planus and hyperkeratosis on the Merkel cell-neurite complex,healthy oral mucosal epithelium and lesional oral mucosal epithelium of lichen planus and hyperkeratosis patients were stained by immunohistochemistry(the avidin-biotin-peroxidase complex and double immunofluorescence methods)using pan cytokeratin,20(K20,a Merkel cell marker),and neurofilament 200(NF200,a myelinated Ab-and Ad-nerve fibre marker)antibodies.NF200-immunoreactive(ir)nerve fibres in healthy tissues and in the lesional oral mucosa epithelium of lichen planus and hyperkeratosis were counted and statistically analysed.In the healthy oral mucosa,K20-positive Merkel cells with and without close association to the intraepithelial NF200-ir nerve fibres were detected.In the lesional oral mucosa of lichen planus and hyperkeratosis patients,extremely rare NF200-ir nerve fibres were detected only in the lamina propria.Compared with healthy tissues,lichen planus and hyperkeratosis tissues had significantly decreased numbers of NF200-ir nerve fibres in the oral mucosal epithelium.Lichen planus and hyperkeratosis were associated with the absence of Ab-nerve endings in the oral mucosal epithelium.Thus,we conclude that mechanosensation mediated by the Merkel cell-neurite complex in the oral mucosal epithelium is impaired in lichen planus and hyperkeratosis. | Daniela Calderon Carrion Yiiksel Korkmaz Britta Cho Marion Kopp Wilhelm Bloch Klaus Addicks Wilhelm Niedermeier | 2016 | International Journal of Oral Science2016，8，1： | 2 |
| 2 | Immunolocalization of inducible and constitutive nitric oxide synthases in human bladder cancer显示文摘 | Theo Klotz Wilhelm Bloch Georg Jacobs Sandra Niggemann Udo Engelmann Klaus Addicks | 1999 | Urology1999，，3： | 1 |
| 3 | The growth of Staphylococcus aureus and Escherichia coli in low-direct current electric fields显示文摘Electrical potentials up to 800 mV can be observed between different metallic dental restorations. These potentials produce fields in the mouth that may interfere with microbial communities. The present study focuses on the impact of different electric field strengths(EFS) on the growth of Staphylococcus aureus(ATCC 25923) and Escherichia coli(ATCC 25922) in vitro. Cultures of S. aureus and E. coli in fluid and gel medium were exposed to different EFS. Effects were determined by calculation of viable counts and measurement of inhibition zones. In gel medium, anodic inhibition zones for S. aureus were larger than those for E. coli at all field strength levels. In fluid medium, the maximum decrease in the viable count of S. aureus cells was at 10 V·m21. Field-treated S. aureus cells presented ruptured cell walls and disintegrated cytoplasm. Conclusively, S. aureus is more sensitive to increasing electric field strength than E. coli. | Dunya Zituni Heidi Schtt-Gerowitt Marion Kopp Martin Krnke Klaus Addicks Christian Hoffmann Martin Hellmich Franz Faber Wilhelm Niedermeier | 2014 | International Journal of Oral Science2014，6，1： | 1 |
| 4 | β_1整合素基因缺失对G_i蛋白分布的影响显示文摘目的 了解跨膜蛋白 β1整合素对信号分子G蛋白细胞内构型的影响 ,为进一步研究 β1整合素在信号传导中的作用打下基础 .方法 采用荧光双标记及重叠合显微镜分析技术 ,观察野生型胚胎干细胞系 (D3细胞 ) ,β1整合素基因敲除胚胎干细胞 (G2 0 1细胞 ) ,β1整合素基因敲除后再植入胚胎干细胞 (G2 0 1N)及小鼠胚胎心肌细胞中多种G蛋白 (Gαs,Gαi1-3 ,Gαo,Gαi/o/t/z)细胞内构型特点和差异 .结果 Gαs,Gαo,Gαi/o/t/z在上述几种细胞系中均呈弥漫性分布 ,未能发现明显差异 ,与此不同的是Gαi1-3 在D3细胞源性心肌细胞内呈网状分布 ,在非心肌细胞内呈线条状分布 ,而在G2 0 1细胞中Gi 上述特异性构型被打乱 ,呈近似于弥漫样分布 ,值得注意的是在β1整合素基因重新恢复的G2 0 1N细胞Gi构型又呈网样或线条样分布 ,与野生型胚胎干细胞中Gi 的构型相一致 .结论 β1整合素能够影响信号分子Gi蛋白的细胞内定位 ,推测β1整合素可通过改变Gi 的分布而影响Gi | 韩捷 Wilhel m Bloch Michael Welthar Klaus Addicks | 2001 | 第四军医大学学报2001，22，15： | 0 |
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