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2篇 您的检索式：作者名="Kenneth Dorko"

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		题名	作者	年代	出处	被引量
	1	Mechanisms of acetaminophen-induced cell death in primary human hepatocytes显示文摘	Yuchao Xie Mitchell R. McGill Kenneth Dorko Sean C. Kumer Timothy M. Schmitt Jameson Forster Hartmut Jaeschke	2014	Toxicology and Applied Pharmacology2014，，：	1
	2	Lack of direct cytotoxicity of extracellular ATP against hepatocytes:role in the mechanism of acetaminophen hepatotoxicity显示文摘Background:Acetaminophen(APAP)hepatotoxicity is a major cause of acute liver failure in many countries.Mechanistic studies in mice and humans have implicated formation of a reactive metabolite,mitochondrial dysfunction and oxidant stress as critical events in the pathophysiology of APAP-induced liver cell death.It was recently suggested that ATP released from necrotic cells can directly cause cell death in mouse hepatocytes and in a hepatoma cell line(HepG2).Aim:To assess if ATP can directly cause cell toxicity in hepatocytes and evaluate their relevance in the human system.Methods:Primary mouse hepatocytes,human HepG2 cells,the metabolically competent human HepaRG cell line and freshly isolated primary human hepatocytes were exposed to 10-100μM ATP or ATγPin the presence or absence of 5-10 mM APAP for 9-24 h.Results:ATP or ATγP was unable to directly cause cell toxicity in all 4 types of hepatocytes.In addition,ATP did not enhance APAP-induced cell death observed in primary mouse or human hepatocytes,or in HepaRG cells as measured by LDH release and by propidium iodide staining in primary mouse hepatocytes.Furthermore,addition of ATP did not cause mitochondrial dysfunction or enhance APAP-induced mitochondrial dysfunction in primary murine hepatocytes,although ATP did cause cell death in murine RAW macrophages.Conclusions:It is unlikely that ATP released from necrotic cells can significantly affect cell death in human or mouse liver during APAP hepatotoxicity.Relevance for patients:Understanding the mechanisms of APAP-induced cell injury is critical for identifying novel therapeutic targets to prevent liver injury and acute liver failure in APAP overdose patients.	Yuchao Xie Benjamin L.Woolbright Milan Kos Mitchell R.McGill Kenneth Dorko Sean C.Kumer Timothy M.Schmitt Hartmut Jaeschke	2015	Journal of Clinical & Translational Research2015，1，2：	0

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