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| 1 | 波前像差引导的LASIK手术的临床效果观察显示文摘目的 :观察波前像差引导的LASIK手术矫正近视性散光的效果。方法 :2 8例 35只眼 ,平均术前球镜屈光度为 (- 4.8± 2 .3)D ,柱镜屈光度为 (- 1.1± 0 .9)D。手术前和手术后均使用Tscherning像差计测量 ,分析波前像差。应用 1.0mm光斑、频率 2 0 0Hz的飞点扫描准分子激光进行屈光手术。结果 :术后 3个月 ,6 8.0 %的眼屈光度在± 0 .5D(正视眼 )内 ,93.5 %眼在± 1.0D内。 93.5 %的裸眼视力为 2 0 / 2 0或更好。裸眼超常视力(BSCVA在 2 0 / 10或更好 )占 16 .0 %。高阶像差 (球差 ,彗差 )矫正不足 ,而波前像差全部均方根增加值为 1.44± 0 .74,彗差的矫正好于球差。结论 :波前像差引导的LASIK手术是一种很可靠的技术 ,可有效地矫正屈光不正 ,改善视觉敏感度 ,提高视觉质量 ,尤其是提高夜间视力的视觉质量。 | Michael Mrochen Maik Kaemmerer Theo Seiler | 2001 | 眼视光学杂志2001，3，2： | 63 |
| 2 | 波前像差引导的LASIK术矫正眼球像差显示文摘目的 :观察波前像差引导的LASIK术矫正人眼像差后的效果 ,以确定人眼是否可获得超过一般的视敏度。方法 :波前像差引导的LASIK手术对 15只眼矫正近视和散光 ,同时矫正眼球的像差。结果 :手术后 1个月时 ,4只眼 (占 2 7% )获得了 2 0 /10以上的超常视力。波前像差的均方根值从 0 .6增加到 2 .3,与视觉敏感度的提高呈显著性相关 (P <0 .0 5 )。结论 :像差的矫正虽然还不能最令人满意 ,但这些结果表明 ,眼球的像差使人眼视敏度受限制 ,而通过手术矫正可获得超过一般的视敏度。 | Theo Seiler Michael Mrochen Maik Kaemmerer | 2001 | 眼视光学杂志2001，3，2： | 11 |
| 3 | 肠的障碍: 分子的小径和修饰词显示文摘 The gastrointestinal tract is frequently challenged by pathogens/antigens contained in food and water and the intestinal epithelium must be capable of rapid regeneration in the event of tissue damage. Disruption of the intestinal barrier leads to a number of immune-mediated diseases, including inflammatory bowel disease, food allergy, and celiac disease. The intestinal mucosa is composed of different types of epithelial cells in specific barrier functions. Epithelial cells control surfaceassociated bacterial populations without disrupting the intestinal microflora that is crucial for host health. They are also capable of modulating mucosal immune system, and are thus essential in maintaining homeostasis in the gut. Thus, the regulation of intestinal epithelial homeostasis is crucial for the maintenance of the structure of the mucosa and the defensive barrier functions. Recent studies have demonstrated that multiple molecular pathways are involved in the regulation of intestinal epithelial cell polarity. These include the Wnt, Notch, Hippo, transforming growth factor-β(TGF-β)/bone morphogenetic protein(BMP) and Hedgehog pathways, most of which were identified in lower organisms where they play important roles during embryogenesis. These pathways are also used in adult organisms to regulate multiple self-renewing organs. Understanding the interactions between these molecular mechanisms and intestinal barrier function will therefore provide important insight into the pathogenesis of intestinal-based immune-mediated diseases. | Min Kyung Jeon Christina Klaus Elke Kaemmerer Nikolaus Gassler | 2013 | World Journal of Gastrointestinal Pathophysiology2013，4，4： | 10 |
| 4 | Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity显示文摘AIM:To investigate the role of acyl-CoA synthetase 5(ACSL5)activity in Wnt signaling in intestinal surface epithelia.METHODS:Several cell lines were used to investigate the ACSL5-dependent expression and synthesis of Wnt2B,a mitochondrially expressed protein of the Wnt signaling family.Wnt activity was functionally assessed with a luciferase reporter assay.ACSL5-related biochemical Wnt2B modifications were investigatedwith a modified acyl-exchange assay.The findings from the cell culture models were verified using an Apcmin/+mouse model as well as normal and neoplastic diseased human intestinal tissues.RESULTS:In the presence of ACSL5,Wnt2B was unable to translocate into the nucleus and was enriched in mitochondria,which was paralleled by a significant decrease in Wnt activity.ACSL5-dependent S-palmitoylation of Wnt2B was identified as a molecular reason for mitochondrial Wnt2B accumulation.In cell culture systems,a strong relation of ACSL5 expression,Wnt2B palmitoylation,and degree of malignancy were found.Using normal mucosa,the association of ACSL5 and Wnt2B was seen,but in intestinal neoplasias the mechanism was only rudimentarily observed.CONCLUSION:ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity.The molecular pathway is probably relevant for intestinal homeostasis,overwhelmed by other pathways in carcinogenesis. | Christina Klaus Ursula Schneider Christian Hedberg Anke K Schütz Jürgen Bernhagen Herbert Waldmann Nikolaus Gassler Elke Kaemmerer | 2014 | World Journal of Gastroenterology2014，20，40： | 4 |
| 5 | Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C显示文摘AIM:To investigate whether human acyl-CoA synthetase 5(ACSL5) is sensitive to the ACSL inhibitor triacsin C.METHODS:The ACSL isoforms ACSL1 and ACSL5 from rat as well as human ACSL5 were cloned and recombinantly expressed as 6xHis-tagged enzymes.Ni 2+-affinity purified recombinant enzymes were assayed at pH 7.5 or pH 9.5 in the presence or absence of triacsin C.In addition,ACSL5 transfected CaCo2 cells and intestinal human mucosa were monitored.ACSL5 expression in cellular systems was verified using Western blot and immunofluorescence.The ACSL assay mix included TrisHCl(pH 7.4),ATP,CoA,EDTA,DTT,MgCl 2,[9,103 H] palmitic acid,and triton X-100.The 200 μL reaction was initiated with the addition of solubilized,purified recombinant proteins or cellular lysates.Reactions were terminated after 10,30 or 60 min of incubation with Doles medium.RESULTS:Expression of soluble recombinant ACSL proteins was found after incubation with isopropyl betaD-1-thiogalactopyranoside and after ultracentrifugation these were further purified to near homogeneity with Ni 2+-affinity chromatography.Triacsin C selectively and strongly inhibited recombinant human ACSL5 protein at pH 7.5 and pH 9.5,as well as recombinant rat ACSL1(sensitive control),but not recombinant rat ACSL5(insensitive control).The IC50 for human ACSL5 was about 10 μmol/L.The inhibitory triacsin C effect was similar for different incubation times(10,30 and 60 min) and was not modified by the N-or C-terminal location of the 6xHis-tag.In order to evaluate ACSL5 sensitivity to triacsin C in a cellular environment,stable human ACSL5 CaCo2 transfectants and mechanically dissected normal human intestinal mucosa with high physiological expression of ACSL5 were analyzed.In both models,ACSL5 peak activity was found at pH 7.5 and pH 9.5,corresponding to the properties of recombinant human ACSL5 protein.In the presence of triacsin C(25 μmol/L),total ACSL activity was dramatically diminished in human ACSL5 transfectants as well as in ACSL5-rich human intestinal mucosa.CONCLUSION:The data strongly indicate that human ACSL5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms. | Elke Kaemmerer Anne Peuscher Andrea Reinartz Christian Liedtke Ralf Weiskirchen Jürgen Kopitz Nikolaus Gassler | 2011 | World Journal of Gastroenterology2011，17，44： | 3 |
| 6 | Beta-7 integrin controls enterocyte migration in the small intestine显示文摘AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total small intestine was isolated,dissected,and used for morphometrical studies.Brd U-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum,jejunum,and ileum of each animal.The outer most Brd U-positive cell(cellmax)was determined per hemi-crypt,numerically documented,and statistically analysed.RESULTS:Integrins containing the beta-7-chain were exclusively expressed on leukocytes.In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased.Moreover,the Peyer’s patches of beta-7integrin-deficient mice appeared hypoplastic.In beta-7integrin-deficient mice the location of cellmax was found in a higher position than it was the case for the controls.The difference was already detected at 4 h after Brd U application,but significantly increased with time(40 h after Brd U injection)in all small intestinal segments investigated,i.e.,duodenum,jejunum,and ileum.Migration of small intestinal enterocytes was different between the experimental groups measured by cellmax locations.CONCLUSION:The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer. | Elke Kaemmerer Paula Kuhn Ursula Schneider Thomas Clahsen Min Kyung Jeon Christina Klaus Julia Andruszkow Michael Hrer Sabine Ernst Angela Schippers Norbert Wagner Nikolaus Gassler | 2015 | World Journal of Gastroenterology2015，21，6： | 3 |
| 7 | Neoadjuvant peptide receptor radionuclide therapy for an inoperable neuroendocrine pancreatic tumor显示文摘Pancreatic endocrine tumors are rare but are among the most common neuroendocrine neoplasms of the abdomen.At diagnosis many of them are already advanced and diff icult to treat.We report on an initially inoperable malignant pancreatic endocrine tumor in a 33-year-old woman,who received neoadjuvant peptide receptor radionuclide therapy(PRRT)as firstline treatment.This resulted in a signif icant downstaging of the tumor and allowed its subsequent complete surgical removal.Follow-up for eighteen months revealed a complete remission.This is the first report on neoadjuvant PRRT in a neuroendocrine neoplasm with subsequent successful complete resection. | Daniel Kaemmerer Vikas Prasad Wolfgang Daffner Dieter Hrsch Günter Klppel Merten Hommann Richard P Baum | 2009 | World Journal of Gastroenterology2009，15，46： | 2 |
| 8 | Molecular classification of colorectal carcinomas:The genotype-to-phenotype relation显示文摘Colorectal carcinomas(CRCs)are frequently found in industrialized countries and lead to a high incidence of malignancy-related mortality.Defined by histomorphological features,CRCs and their pre-invasive lesions are quite heterogeneous.The underlying molecular mechanisms include genomic instability,genomic mutation of tumor suppressor genes or oncogenes,epigenetic changes,and the microRNA network.The molecular mechanisms are guided by repeated clonal selections.The genotype-to-phenotype relation is assumed to be the great challenge of cancer research and the development of effective targeted therapies.At present a strong genotype-to-phenotype relation is characterized only for a minority of CRCs.Consequently,the molecular characterization of CRCs is essential to interpret histological patterns and to identify prognostic groups as well as patients for targeted therapy. | Elke Kaemmerer Christina Klaus Min Kyung Jeon Nikolaus Gassler | 2013 | World Journal of Gastroenterology2013，19，45： | 2 |
| 9 | Intestinal acyl-CoA synthetase 5: Activation of long chain fatty acids and behind显示文摘The intestinal mucosa is characterized by a high complexity in terms of structure and functions and allows for a controlled demarcation towards the gut lumen.On the one hand it is responsible for pulping and selective absorption of alimentary substances ensuring the immunological tolerance,on the other hand it prevents the penetration of micro-organisms as well as bacterial outgrowth.The continuous regeneration of surface epithelia along the crypt-villus-axis in the small intestine is crucial to assuring these various functions.The core phenomena of intestinal epithelia regeneration comprise cell proliferation,migration,differentiation,and apoptosis.These partly contrarily oriented processes are molecularly balanced through numerous interacting signaling pathways like Wnt/β-catenin,Notch and Hedgehog,and regulated by various modifying factors.One of these modifiers is acyl-CoA synthetase 5(ACSL5).It plays a key role in de novo lipid synthesis,fatty acid degradation and membrane modifications,and regulates several intestinal processes,primarily through different variants of protein lipidation,e.g.,palmitoylation.ACSL5 was shown to interact with proapoptotic molecules,and besides seems to inhibit proliferation along the crypt-villus-axis.Because of its proapoptotic and antiproliferative characteristics it could be of significant relevance for intestinal homeostasis,cellular disorder and tumor development. | Christina Klaus Min Kyung Jeon Elke Kaemmerer Nikolaus Gassler | 2013 | World Journal of Gastroenterology2013，19，42： | 2 |
| 10 | Ocular optical aberrations after photorefractive keratectomy for myopia and myopic astigmatism显示文摘 | Seiler T Kaemmerer M Mierdel P | 2000 | Arch Ophthalmol2000，118，1： | 1 |
| 11 | Increasead higher-order optical aberrations after laser refractive surgery显示文摘 | Mrochen M Kaemmerer M Mierdel P | 2001 | J Caract refract Surg2001，27，3： | 1 |
| 12 | Effects of photorefractive keratectomy and cataract surgery on ocular optical errors of higher order显示文摘 | P. Mierdel Maik Kaemmerer Hans-Eberhard Krinke Theo Seiler | 1999 | Graefe’s Archive for Clinical and Experimental Ophthalmology1999，，9： | 1 |
| 13 | Lipid peroxidation products reduce lysosomal protease activities in human retinal pigment epithelial cells via two different mechanisms of action显示文摘 | Tim U. Krohne Elke Kaemmerer Frank G. Holz Jürgen Kopitz | 2009 | Experimental Eye Research2009，，2： | 1 |
| 14 | Ocular optical aberrations after photorefractive keratectomy for myopia and myopic astigmatism 显示文摘 | Seiler T Kaemmerer M Mierdel P | 2000 | Arch Ophthalmol2000，118，1： | 1 |
| 15 | Wavefront guided laser in situ keratomileusis: early results in three eyes显示文摘 | MROCHEN M KAEMMERER M SEILER T | 2000 | J Refract Surg2000，16，： | 1 |
| 16 | Increased higher-order optical aberrations after laser refractive surgery:a problem of subclinical decentration显示文摘 | Mrochen M Kaemmerer M Mierdel P | | 0，，03： | 1 |
| 17 | Ocular optical aberrations after photorefractive keratectomy for myopia and myopic astigmatism显示文摘 | Seller T Kaemmerer M Mierdel P | 2000 | Arch Ophthalmol2000，118，1： | 1 |
| 18 | Measuring the perceived support for innovation in organization 显示文摘 | Siegel S Kaemmerer W | 1986 | Journal of Applied Psychology1986，，63： | 1 |
| 19 | Clinical results of wavefront guided LASIK at 3 months after surgery 显示文摘 | Mrochen M Kaemmerer M Seiler T | 2001 | J Cataract Refract Surg2001，27，2： | 1 |
| 20 | Oligonucleotide fingerprinting of tomato DNA 显示文摘 | Kaemmer D Weising K Beyermann B | 1995 | Plant Breed1995，114，： | 1 |
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