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| 1 | 地被菊花Fall Color体细胞胚途径再生、遗传转化及转基因植株的抗寒性检测显示文摘【目的】培育耐寒性强的地被菊花[Dendranthemagrandiflorum(Ramat.)Kitamura]新材料。【方法】以FallColor品种的幼嫩叶片为外植体,探讨胚状体诱导所需的植物生长调节剂浓度和诱导培养时间等条件。【结果】叶片外植体在添加0.75mg·L-12,4-D的诱导培养基上诱导15d,再进行分生培养,不仅能够诱导胚性愈伤组织形成,还能够诱导胚状体发生,并进一步诱导芽再生,最终93%的供试外植体通过胚状体途径获得芽的再生。通过根癌农杆菌介导法将35S启动子驱动的逆境诱导转录因子DREB1A基因导入该品种。转化株在低温下的种子发芽率、扦插苗生长以及植株露地越冬生长状况等方面都明显优于对照。【结论】本研究成功地建立了地被菊花FallColor体细胞胚再生途径,并成功地获得了具有越冬耐性的地被菊花转化株系。 | 洪波 仝征 李邱华 马超 KASUGA Mie YAMAGUCHI-SHINOZA KIKaziko 高俊平 | 2006 | 中国农业科学2006，39，7： | 28 |
| 2 | AtDREB1A基因在菊花中的异源表达提高了植株对干旱和盐渍胁迫的耐性显示文摘将携带有AtDREBlA基因,并以35S或rd29A启动子驱动的植物表达载体转入地被菊花(Dendranthema grandiflorum)的粉色品种‘Fall color’.与野生型相比,35S:DREBIA转基因植株表现出严重的生长抑制,而rd29A:DREB1A植株生长正常.RT-PCR检测表明,在胁迫条件下, AtDREB1A基因在35S:DREB1A转基因植株中呈现组成型过量表达,而在rd29A:DREB1A植株中则是受胁迫诱导型过量表达.这两种启动子驱动的转基因植株对干旱和盐溃胁迫都表现出较强的耐性,其中rd29A:DREB1A植株耐性显著强于35S:DREB1A植株.rd29A:DREB1A植株中的脯氨酸含量和SOD活性都强烈地被胁迫诱导升高,且高于35S:DREB1A植株.这些结果表明,在地被菊花中表达AtDREB1A基因可以提高植株对干旱和盐渍胁迫的耐性,同时这些耐性的升高可能与脯氨酸含量和SOD活性的上升有关. | 洪波 仝征 马男 李建科 Mie Kasuga Kazuko Yamaguchi-Shinozaki 高俊平 | 2006 | 中国科学(C辑)2006，36，3： | 25 |
| 3 | 根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化显示文摘以地被菊花(Dendranthema grandiflorum cv.White Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR-Southern检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5~0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。 | 洪波 张常青 李邱华 高俊平 KASUGA Mie SHINOZAKI-YAMAGUCHI Kazuko | 2005 | 农业生物技术学报2005，13，3： | 20 |
| 4 | Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance显示文摘DNA cassette containing an AtDREB1A cDNA and a nos terminator,driven by a cauli- flower mosaic 35S promoter,or a stress-inducible rd29A promoter,was transformed into the ground cover chrysanthemum(Dendranthema grandiflorum)'Fall Color'genome.Compared with wild type plants,severe growth retardation was observed in 35S:DREB1A plants,but not in rd29A:DREB1A plants.RT-PCR analysis revealed that,under stress conditions,the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants,but was over-expressed inductively in rd29A:DREB1A plants.The transgenic plants exhibited tolerance to drought and salt stress,and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants.Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions.These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum,and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. | KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko | 2006 | Science China(Life Sciences)2006，49，5： | 18 |
| 5 | A basic helix-loop-helix transcription factor, PhFBH4, regulates flower senescence by modulating ethylene biosynthesis pathway in petunia显示文摘The basic helix-loop-helix(bHLH)transcription factors(TFs)play important roles in regulating multiple biological processes in plants.However,there are few reports about the function of bHLHs in flower senescence.In this study,a bHLH TF,PhFBH4,was found to be dramatically upregulated during flower senescence.Transcription of PhFBH4 is induced by plant hormones and abiotic stress treatments.Silencing of PhFBH4 using virus-induced gene silencing or an antisense approach extended flower longevity,while transgenic petunia flowers with an overexpression construct showed a reduction in flower lifespan.Abundance of transcripts of senescence-related genes(SAG12,SAG29)was significantly changed in petunia PhFBH4 transgenic flowers.Furthermore,silencing or overexpression of PhFBH4 reduced or increased,respectively,transcript abundances of important ethylene biosynthesis-related genes,ACS1 and ACO1,thereby influencing ethylene production.An electrophoretic mobility shift assay showed that the PhFBH4 protein physically interacted with the G-box cis-element in the promoter of ACS1,suggesting that ACS1 was a direct target of the PhFBH4 protein.In addition,ectopic expression of this gene altered plant development including plant height,internode length,and size of leaves and flowers,accompanied by alteration of transcript abundance of the gibberellin biosynthesis-related gene GA2OX3.Our results indicate that PhFBH4 plays an important role in regulating plant growth and development through modulating the ethylene biosynthesis pathway. | Jing Yin Xiaoxiao Chang Takao Kasuga Mai Bui Michael S Reid Cai-Zhong Jiang | 2015 | Horticulture Research2015，2，1： | 7 |
| 6 | Ultrasound-guided vs endoscopic ultrasound-guided fine-needle aspiration for pancreatic cancer diagnosis显示文摘AIM: To clarify the effectiveness and safety of endo- scopic ultrasound-guided fine-needle aspiration (EUS-FNA) for the diagnosis of pancreatic cancer (PC). METHODS: Patients who were diagnosed with unresectable, locally advanced or metastatic PC between February 2006 and September 2011 were selected for this retrospective study. FNA biopsy for pancreatic tumors had been performed percutaneously under extracorporeal ultrasound guidance until October 2009; then, beginning in November 2009, EUS-FNA has been performed. We reviewed the complete medical records of all patients who met the selection criteria for the following data: sex, age, location and size of the targeted tumor, histological and/or cytological findings, details of puncture procedures, time from day of puncture until day of definitive diagnosis, and details of severe adverse events. RESULTS: Of the 121 patients who met the selection criteria, 46 had a percutaneous biopsy (Group A) and 75 had an EUS-FNA biopsy (Group B). Adequate cytological specimens were obtained in 42 Group A patients (91.3%) and all 75 Group B patients (P=0.0192), and histological specimens were obtained in 41 Group A patients (89.1%) and 65 Group B patients (86.7%). Diagnosis of malignancy by cytology was positive in 33 Group A patients (78.6%) and 72 Group B patients (94.6%) (P=0.0079). Malignancy by both cytology and pathology was found in 43 Group A (93.5%) and 73 Group B (97.3%) patients. The mean period from the puncture until the cytological diagnosis in Group B was 1.7 d, which was significantly shorter than that in Group A (4.1 d) (P < 0.0001). Severe adverse events were experienced in two Group A patients (4.3%) and in one Group B patient (1.3%). CONCLUSION: EUS-FNA, as well as percutaneous needle aspiration, is an effective modality to obtain cytopathological confirmation in patients with advanced PC. | Masato Matsuyama Hiroshi Ishii Kensuke Kuraoka Seigo Yukisawa Akiyoshi Kasuga Masato Ozaka Sho Suzuki Kouichi Takano Yuko Sugiyama Takao Itoi | 2013 | World Journal of Gastroenterology2013，19，15： | 5 |
| 7 | Treatment strategy for rectal carcinoids: A clinicopathological analysis of 229 cases at a single cancer institution显示文摘 | Akiyoshi Kasuga Akiko Chino Naoyuki Uragami Teruhito Kishihara Masahiro Igarashi Rikiya Fujita Noriko Yamamoto Masashi Ueno Masatoshi Oya Tetsuichiro Muto | 2012 | Journal of Gastroenterology and Hepatology2012，，12： | 2 |
| 8 | Construction of a Butterfly Web Bridge显示文摘 | Kenichiro Ashizuka Kenji Miyamoto Kenichi Kata Akio Kasuga | 2014 | Journal of Civil Engineering and Architecture2014，8，11： | 2 |
| 9 | A high quality surface finish grinding process to produce total reflection mirror for x-ray fluorescence analysis显示文摘Total reflection x-ray fluorescence analysis is applied to trace element detection in liquid for effective environmental monitoring.This analytical approach requires x-ray total reflection mirrors.In order to achieve high sensitivity element detection,the mirrors require high surface quality for high x-ray reflectivity.Surface finishing for x-ray mirrors is typically conducted through a series of abrasive processes,such as grinding and polishing,and is thus time consuming.The purpose of this study is to streamline and enhance the surface finishing process based on unique high quality grinding techniques for the production of x-ray total reflection mirrors. | Hitoshi Ohmori Shinjiro Umezu Yunji Kim Yoshihiro Uehara Hiroshi Kasuga Teruko Kato Nobuhide Itoh Syuhei Kurokawa Takayuki Kusumi Yugo Sugawara Shinsuke Kunimura | 2020 | International Journal of Extreme Manufacturing2020，2，1： | 2 |
| 10 | Clinical characterization of gastric lesions initially diagnosed as low‐grade adenomas on forceps biopsy显示文摘 | Akiyoshi Kasuga Yorimasa Yamamoto Junko Fujisaki Kazuhisa Okada Masami Omae Akiyoshi Ishiyama Toshiaki Hirasawa Akiko Chino Tomohiro Tsuchida Masahiro Igarashi Etsuo Hoshino Noriko Yamamoto Minoru Kawaguchi Rikiya Fujita | 2012 | Digestive Endoscopy2012，，5： | 2 |
| 11 | Long-term outcomes of endoscopic submucosal dissection for undifferentiated-type early gastric cancer显示文摘 | K. Okada J. Fujisaki T. Yoshida H. Ishikawa T. Suganuma A. Kasuga M. Omae M. Kubota A. Ishiyama T. Hirasawa A. Chino M. Inamori Y. Yamamoto N. Yamamoto T. Tsuchida Y. Tamegai A. Nakajima E. Hoshino M. Igarashi | 2012 | Endoscopy2012，，02： | 2 |
| 12 | Why is the coexistence of gastric cancer and duodenal ulcer rare? Examination of factors related to both gastric cancer and duodenal ulcer显示文摘 | Hideyuki Ubukata Hiroyuki Nagata Takanobu Tabuchi Satoru Konishi Teruhiko Kasuga Takafumi Tabuchi | 2011 | Gastric Cancer2011，，1： | 2 |
| 13 | Two transcription factors, DREB 1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and lowtemperature-responsive gene expression respectively in Arabidopsis显示文摘 | LIU Q KASUGA M SAKUMA Y ABE H MIURA S YAMAQUCHI-SHINOZAKI K SHINOZAKI K | 1998 | Plant Cell1998，10，8： | 1 |
| 14 | RBP4, an unexpected adipo-kine显示文摘 | Tamori Y Sakaue H Kasuga M | 2006 | Nat Med2006，12，2： | 1 |
| 15 | A combination of the Arabidopsis DREB1A gene and stress-inducible rd29Apromoter improved drought-and low-temperature stress tolerance in tobacco by gene transfer显示文摘 | Kasuga M Miura S Shinozaki ect | 2006 | Plant and cell physiology2006，45，： | 1 |
| 16 | Two tranription factors,DREB1 and DREB2,with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression,respectively,in Arabidopsis显示文摘 | Liu Q Kasuga M Sakuma Y | 1998 | Plant Cell1998，10，： | 1 |
| 17 | Leisure- time physical activity and breast cancer risk by hormone receptor status; Ef- fective life periods and exercise intensity 显示文摘 | Suzuki R Iwasaki M Kasuga Y | 2010 | Cancer Causes Control2010，21，11： | 1 |
| 18 | Formation of titanium oxide nanotube 显示文摘 | Kasuga T Hiramatsu M Hoson A | 1998 | Langmuir1998，14，： | 1 |
| 19 | Toxicity eval- uation of road dust in the runoff process using a ben- thic ostraeod Heterocypris incongruens 显示文摘 | Watanabe H Nakajima F Kasuga I | 2011 | Science of the Total Environment2011，409，12： | 1 |
| 20 | Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding do- main, separate two ceUular signal transduction pathways in drought and low temperature-responsive gene expression, respec- tively, in Arabidopsis 显示文摘 | Liu Q Kasuga M Sakuma Y | 1998 | Plant Cell1998，10，8： | 1 |
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