|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes显示文摘To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PGR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation. | YINGCHEN ZHIXuHE AILIANLIU KAIWANG WENWEIMAO JIANKINCHU YONGLU ZHENGFUFANG YINGTANGSHI QINGZHANGYANG DAYUANCHEN MINKANGWANG JINSONGLI SHAOLIANGHUANG XIANGYINKONG YAOZHOUSHI ZHIQIANGWANG JIAHuIXIA ZHIGAOLONG ZHIGANGXUE WENXIANGDING HUIZHENSHENG | 2003 | Cell Research2003，13，4： | 56 |
| 2 | Synthesis of Metal Porphyrins Tailed with Salicylic Acid and their Interaction with Bovine Serum Albumin显示文摘A synthetic method of porphyrins tailed with salicylic substituents is described.Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2-5. These new compounds were confirmed by ^1H NMR, IR, UV-vis, MS and elemental analysis, and observed their interaction with bovine serum albumin (BSA) in fluorescence spectrum. | TaoJIA KaiWANG YiMeiZHAO ZaoYingLI | 2004 | Chinese Chemical Letters2004，15，3： | 2 |
| 3 | A Statistical Approach Designed for Finding Mathematically Defined Repeats in Shotgun Data and Determining the Length Distribution of Clone-Inserts显示文摘The large amount of repeats, especially high copy repeats, in the genomes of higher animals and plants makes whole genome assembly (WGA) quite difficult. In order to solve this problem, we tried to identify repeats and mask them prior to assembly even at the stage of genome survey. It is known that repeats of different copy number have different probabilities of appearance in shotgun data, so based on this principle, we constructed a statistical model and inferred criteria for mathematically defined repeats (MDRs) at different shotgun coverages. According to these criteria, we developed software MDRmasker to identify and mask MDRs in shotgun data. With repeats masked prior to assembly, the speed of assembly was increased with lower error probability. In addition, clone-insert size affects the accuracy of repeat assembly and scaffold construction. We also designed length distribution of clone-inserts using our model. In our simulated genomes of human and rice, the length distribution of repeats is different, so their optimal length distributions of clone-inserts were not the same. Thus with optimal length distribution of clone-inserts, a given genome could be assembled better at lower coverage. | LanZhong KunlinZhang XiangangHuang PeixiangNi YujunHan KaiWang JunWang SonggangLi | 2003 | Genomics, Proteomics & Bioinformatics2003，1，1： | 1 |
| 4 | New superhard carbon phases between graphite and diamond显示文摘 | Chaoyu He Lizhong Sun Chunxiao Zhang Xiangyang Peng Kaiwang Zhang Jianxin Zhong | 2012 | Solid State Communications2012，，16： | 1 |
| 5 | ST-scale as a novel amino acid descriptor and its application in QSAM of peptides and analogues显示文摘 | YANG Li SHU Mao MA Kaiwang | 2010 | Amino acids2010，38，3： | 1 |
| 6 | ST-scale as a novel aminoacid descriptor and its application in QSAM of peptides and analogues显示文摘 | YANG Li SHU Mao MA Kaiwang | 2010 | Amino Acids2010，38，： | 1 |
| 7 | 2‐(Dinitromethylene)‐1‐nitro‐1, 3‐diazacyclopentane‐Based Energetic Salts显示文摘 | JinhongSong ZhimingZhou DanCao HaifengHuang LixuanLiang KaiWang JunZhang | 2012 | Z anorg allg Chem2012，，5： | 1 |
| 8 | Distinct effect of Chrysanthemum indicum Linné extracts on isoproterenol-inducedgrowth of human hepatocellular carcinoma cells显示文摘 | Aihua Yuan Zongfang Li Xinqiu Li Shanyong Yi Shukui Wang Kaiwang Shi Jianmin Bian | 2009 | Oncology Reports2009，，6： | 1 |
| 9 | Association of tumor necrosis factor‐α gene promoter polymorphism at sites ‐308 and ‐238 with non‐alcoholic fatty liver disease: A meta‐analysis显示文摘 | Ji‐kaiWang Zhe‐weiFeng Ying‐chunLi Qing‐yanLi Xing‐yongTao | 2012 | Journal of Gastroenterology and Hepatology2012，，4： | 1 |
| 10 | Preliminary result of the grass-tree system for rehabilitation of severely eroded red soils显示文摘 | Lin Kaiwang Tiao Xiao | 1998 | Advanced in Geoecology1998，，31： | 1 |
| 11 | Comprehensive Management Measures for Chicken Rearing in Tea Gardens in Karst Region显示文摘In karst region,industrial development is closely related to rocky desertification control. Using abundant natural resources in karst areas to develop chicken rearing in tea gardens can not only make full use of natural resources and alleviate the contradiction between development and governance in rocky desertification areas but also produce good quality chicken. In this paper,the comprehensive management measures related to chicken farming in tea gardens in karst region were elaborated,in order to provided certain guiding significance for practical production. | Peiqiang DUAN Fangyan PAN Kaiwang GUO Xue HAN | 2019 | Asian Agricultural Research2019，11，4： | 0 |
| 12 | Analysis of the different locomotive wireless multi-traction synchronous control systems for heavy-haul combined trains and their performance显示文摘This paper looks at the heavy-haul combined train composed of different types of locomotives and its distributed power control system with a combination of 800 MHz and TD-LTE wireless communication.It analyses some key characteristic parameters that affect the synchronism and communication of the differential wireless multi-traction synchronous control systems for heavy-haul combined trains.At the same time,in order to reduce the latency of instruction and information transfer between different types of locomotives,improve the time-limit certainty of wireless transmission and optimize the control quality of multi-traction control systems for heavy-haul combined trains,a synchronism optimization strategy based on the Markov decision process on the basis of Petri networkconstruction is proposed.Relevant experiments and tests are carried out to verify the effectiveness of the synchronism optimization of the control system,which provides a guarantee for improving the differential wireless multi-traction synchronous control system for combined trains and optimizing train control. | Wei Li Chunyang Chen Hui Li KaiWang Liang Xie Wenlu Zhang | 2020 | Transportation Safety and Environment2020，2，3： | 0 |
| 13 | Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells显示文摘Objective: To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells. Methods: The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with βadrenergic receptor(β 2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK1/2 intracellular activity. Results: Epi transiently activated MAPK/ERK1/2 in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK1/2. Conclusion: Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tumor cells by inhibiting the β2-AR-mediated activation of MAPK/ERK1/2. | Aihua Yuan Zongfang Li Xinqiu Li Shanyong Yi Shukui Wang Kaiwang Shi Jianmin Bian | 2009 | Journal of Nanjing Medical University2009，23，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员