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| 1 | The biological stress of early weaned piglets显示文摘Pigs experience biological stress such as physiological, environmental, and social challenges when weaned from the sow. The process of weaning is one of the most stressful events in the pig's life that can contribute to intestinal and immune system dysfunctions that result in reduced pig health, growth, and feed intake, particularly during the first week after weaning. Technological improvements in housing, nutrition, health, and management have been used to minimize some of the adverse effects of weaning stress, but a greater understanding of the biological impact of stress is needed to improve strategies to overcome weaning stress. The focus of this review paper is to briefly describe how the biological stress associated with weaning impacts intestinal morphology, structure, physiology, and intestinal immune responses that can impact subsequent production efficiencies such as growth, intake, morbidity, and mortality. | Joy M Campbell Joe D Crenshaw Javier Polo | 2013 | Journal of Animal Science and Biotechnology2013，4，2： | 83 |
| 2 | 地下水铀污染的原位微生物还原与固定:在美国能源部田纳西橡树岭放射物污染现场的试验显示文摘总结了美国斯坦福大学和橡树岭国家实验室等在美国能源部田纳西州橡树岭综合试验基地进行的铀污染原位微生物修复阶段性试验结果.本试验利用微生物以乙醇为电子供体还原地下水和沉积物中的六价铀为不溶解的四价铀,使之原位固定化.随后通过加入溶解氧和硝酸盐来试验微生物还原后的地下水层中还原固定态铀的稳定性.通过预处理和长期间隔注入乙醇溶液,地下水中铀浓度从40~60mg·L-1降至0.03mg·L-1以下,达到了美国环保署饮用水的标准.还原的四价铀主要以U(Ⅳ)-Fe复合物的形态存在.结果表明,固定化后的四价铀只有在厌氧条件下才是稳定的,溶解氧和硝酸盐侵入地下水层后会使固定化的四价铀重新氧化为溶解态的六价铀.在试验过程中,采用多种分子生物学方法检测了微生物种群的变化和与铀氧化还原反应有关的功能微生物.本研究表明,在维持试验系统无氧和无硝酸盐的条件下,通过添加乙醇为电子供体可有效地促进地下水中土著功能微生物的活性,从而实现铀的原位还原固定与稳定. | 吴唯民 Jack Carley David Watson 顾宝华 Scott Brooks Shelly D.Kelly Kenneth Kemner Joy D.van Nostrand 吴力游 许玫英 周集中 罗剑 Erick Cardenas 黄家琪 Matthew W.Fields Terence L.Marsh James M.Tiedje Stefan J.Green Joel E.Kostka Peter K.Kitanidis Philip M.Jardme CraigS.Criddle | 2011 | 环境科学学报2011，31，3： | 28 |
| 3 | Science的办刊理念及启示显示文摘为了学习美国Science的办刊理念,笔者深入该刊编辑部,与他们进行了全面交流。认为Science之所以成功,是因为:其一,强大的编辑团队。其专职主编是优秀的学者,基本每期都有他们亲自撰写的社论;多数专职编辑来自国际知名大学,有很高的学术素养;编辑具有很高的独立性。其二,由世界顶级科学家组成的同行评议团队,有高水平的同行评议质量。其三,朴实的办刊目的。它出版跨学科的论文,要求论文有普遍的适用性,要能在某些领域改变人们的思想并推动科学教育的发展。其四,悠久的办刊历史积累,非盈利性的办刊模式及AAAS为后盾。其五,良好的互动交流。通过总结这些经验,提出对我国科技期刊发展的几点建议。 | 石应江 齐国翠 Joy Ma | 2014 | 中国科技期刊研究2014，25，11： | 22 |
| 4 | Sitagliptin in patients with non-alcoholic steatohepatitis: A randomized, placebo-controlled trial显示文摘AIM To evaluate the effect of sitagliptin vs placebo on histologic and non-histologic parameters of nonalcoholic steatohepatitis(NASH).METHODS Twelve patients with biopsy-proven NASH were randomized to sitagliptin(100 mg daily)(n=6)or placebo(n=6)for 24 wk.The primary outcome was improvement in liver fibrosis after 24 wk.Secondary outcomes included evaluation of changes in NAFLD activity score(NAS),individual components of NAS(hepatocyte ballooning,lobular inflammation,and steatosis),glycemic control and insulin resistance[including measurements of glycated hemoglobin(Hb A1C)and adipocytokines],lipid profile including free fatty acids,adipose distribution measured using magnetic resonance imaging(MRI),and thrombosis markers(platelet aggregation and plasminogen activator inhibitor 1 levels).We also sought to determine the correlation between changes in hepatic fat fraction(%)[as measured using the Iterative Decomposition of water and fat with Echo Asymmetry and Least-squares estimation(IDEAL)MRI technique]and changes in hepatic steatosis on liver biopsy.RESULTS Sitagliptin was not significantly better than placebo at reducing liver fibrosis score as measured on liver biopsy(mean difference between sitagliptin and placebo arms,0.40,P=0.82).There were no significant improvements evident with the use of sitagliptin vs placebo for the secondary histologic outcomes of NAS total score as well as for the individual components of NAS.Compared to baseline,those patients who received sitagliptin demonstrated improved Hb A1C(6.7%±0.4%vs 7.9%±1.0%,P=0.02),and trended towards improved adiponectin levels(4.7±3.5μg/m L vs 3.9±2.7μg/m L,P=0.06)and triglyceride levels(1.26±0.43 mmol/L vs 2.80±1.64 mmol/L,P=0.08).However,when compared with placebo,sitagliptin did not cause a statistically significant improvement in Hb A1C(mean difference,-0.7%,P=0.19)nor triglyceride levels(mean difference-1.10mmol/L,P=0.19)but did trend towards improved adiponectin levels only(mean difference,0.60μg/m L,P=0.095).No significant changes in anthropometrics,liver enzymes,other adipocytokines,lipid profile,thrombosis parameters,or adipose distribution were demonstrated.The MRI IDEAL procedure correlated well with steatosis scores obtained on liver biopsy in both groups at baseline and post-treatment,and the Spearman correlation coefficients ranged from r=0.819(baseline)to r=0.878(post-treatment),P=0.002.CONCLUSION Sitagliptin does not improve fibrosis score or NAS after 24 wk of therapy.The MRI IDEAL technique may be useful for non-invasive measurement of hepatic steatosis. | Tisha R Joy Charles A McKenzie Rommel G Tirona Kelly Summers Shannon Seney Subrata Chakrabarti Neel Malhotra Melanie D Beaton | 2017 | World Journal of Gastroenterology2017，23，1： | 18 |
| 5 | Increased hepcidin expression in colorectal carcinogenesis显示文摘AIM:To investigate whether the iron stores regulator hepcidin is implicated in colon cancer-associated anae- mia and whether it might have a role in colorectal car- cinogenesis. METHODS: Mass spectrometry (MALDI-TOF MS and SELDI-TOF MS) was employed to measure hepcidin in urine collected from 56 patients with colorectal cancer. Quantitative Real Time RT-PCR was utilised to determine hepcidin mRNA expression in colorectal cancer tissue. Hepcidin cellular localisation was determined using im- munohistochemistry. RESULTS: We demonstrate that whilst urinary hepcidin expression was not correlated with anaemia it was posi- tively associated with increasing T-stage of colorectal cancer (P < 0.05). Furthermore, we report that hepcidin mRNA is expressed in 34% of colorectal cancer tissue specimens and was correlated with ferroportin repres- sion. This was supported by hepcidin immunoreactivity in colorectal cancer tissue. CONCLUSION: We demonstrate that systemic hepcidin expression is unlikely to be the cause of the systemic anaemia associated with colorectal cancer. However, we demonstrate for the first time that hepcidin is expressed by colorectal cancer tissue and that this may represent a novel oncogenic signalling mechanism. | Douglas G Ward Keith Roberts Matthew J Brookes Howard Joy Ashley Martin Tariq Ismail Robert Spychal Tariq Iqbal Chris Tselepis | 2008 | World Journal of Gastroenterology2008，14，9： | 12 |
| 6 | Heterogeneous molecular catalysts for electrocatalytic CO2 reduction显示文摘This review provides an overview of the literature regarding heterogeneous molecular catalysts for electrochemical CO2 reduction (ECR).Fundamental aspects of the science,including aggregation,electrochemical rate laws,and electrode-catalyst electronic coupling,are discussed to provide a solid foundation on which to design experiments and interpret results.Mechanistic aspects of ECR are presented based on electrokinetic and spectroscopic measurements as well as density functional theory (DFT) calculations.Consensus is improving for electrokinetic measurements,but the redox state of the metal center under reaction conditions and DFT reaction pathways lack agreement in the literature.Concerning the tunable aspects of the molecular catalyst,the impacts of the metal center,ligand substituents,and electrode support on the activity and selectivity toward ECR are presented with an emphasis on those studies that controlled for aggregation and minimized mass-transport limitations.Extended three-dimensional (3D) structures such as polymers,metal-organic frameworks (MOFs),and covalent-organic frameworks (COFs) are discussed as highly tunable architectures that begin to mimic the catalytic pockets of enzyme active sites.To achieve the full potential of these catalysts,design principles must emerge based on a combination of deconvoluting measurements to extract intrinsic catalyst properties and more reliable theoretical calculations to predict reaction pathways. | Nathan Corbin Joy Zeng Kindle Williams Karthish Manthiram | 2019 | Nano Research2019，12，9： | 10 |
| 7 | Development and applications of functional gene microarrays in the analysis of the functional diversity,composition,and structure of microbial communities显示文摘Functional gene arrays(FGAs)are a special type of microarrays containing probes for key genes involved in microbial functional processes,such as biogeochemical cycling of carbon,nitrogen,sulfur,phosphorus,and metals,biodegradation of environmental contaminants,energy processing,and stress responses.GeoChips are considered as the most comprehensive FGAs.Experimentally established probe design criteria and a computational pipeline integrating sequence retrieval,probe design and verification,array construction,data analysis,and automatic update are used to develop the GeoChip technology.GeoChip has been systematically evaluated and demonstrated to be a powerful tool for rapid,specific,sensitive,and quantitative analysis of microbial communities in a high-throughput manner.Several generations of GeoChip have been developed and applied to investigate the functional diversity,composition,structure,function,and dynamics of a variety of microbial communities from different habitats,such as water,soil,marine,bioreactor,human microbiome,and extreme ecosystems.GeoChip is able to address fundamental questions related to global change,bioenergy,bioremediation,agricultural operation,land use,human health,environmental restoration,and ecological theories and to link the microbial community structure to environmental factors and ecosystem functioning. | Zhili HE Joy DVAN NOSTRAND Ye DENG Jizhong ZHOU | 2011 | Frontiers of Environmental Science & Engineering2011，5，1： | 9 |
| 8 | Advances in Vehicular Ad-hoc Networks(VANETs):Challenges and Road-map for Future Development显示文摘Recent advances in wireless communication technologies and auto-mobile industry have triggered a significant research interest in the field of vehicular ad-hoc networks(VANETs) over the past few years. A vehicular network consists of vehicle-to-vehicle(V2V) and vehicle-to-infrastructure(V2I) communications supported by wireless access technologies such as IEEE 802.11 p. This innovation in wireless communication has been envisaged to improve road safety and motor traffic efficiency in near future through the development of intelligent transportation system(ITS). Hence, governments, auto-mobile industries and academia are heavily partnering through several ongoing research projects to establish standards for VANETs. The typical set of VANET application areas, such as vehicle collision warning and traffic information dissemination have made VANET an interesting field of mobile wireless communication. This paper provides an overview on current research state, challenges, potentials of VANETs as well as the ways forward to achieving the long awaited ITS. | Elias C.Eze Si-Jing Zhang En-Jie Liu Joy C.Eze | 2016 | International Journal of Automation and computing2016，13，1： | 8 |
| 9 | Crystal structure of ISG54 reveals a novel RNA binding structure and potential functional mechanisms显示文摘刺激干扰素的基因 56 (ISG56 ) 家庭成员在堵住病毒的复制并且然而,调整细胞的功能起重要作用他们的内在的分子的机制大部分是不清楚的。这里,我们在场 ISG54 的水晶结构,有新奇 RNA 有约束力的结构的 ISG56 家庭蛋白质。结构证明 ISG54 单体有 9 tetratricopeptide 像重复的主题和伙伴到形成交换域的 dimers。C 终端部分合拢进超级螺旋状的结构并且在它的内部表面上有一条广泛地断然控告的核苷酸绑定隧道。EMSA 结果证明 ISG54 明确地绑在一些 RNA,例如 adenylate uridylate (AU ) 富有的 RNA,与或没有 5 ′triphosphorylation。Mutagenesis 和功能的研究证明这个 RNA 有约束力的能力对它的抗病毒的活动重要。我们的结果建议位于这干扰素可诱导的基因的抗病毒的活动下面的新机制 56 家庭成员。 | Zhenlin Yang Huanhuan Liang Qian Zhou Ying Li Haiwei Chen Wen Ye Danying Chen Joy Fleming Hongbing Shu Yingfang Liu | 2012 | Cell Research2012，22，9： | 8 |
| 10 | Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus显示文摘The biodiversity of the mycobiome,an important component of the oral microbial community,and the roles of fungal–bacterial and fungal–immune system interactions in the pathogenesis of oral lichen planus (OLP) remain largely uncharacterized.In this study,we sequenced the salivary mycobiome and bacteriome associated with OLP.First,we described the dysbiosis of the microbiome in OLP patients,which exhibits lower levels of fungi and higher levels of bacteria.Significantly higher abundances of the fungi Candida and Aspergillus in patients with reticular OLP and of Alternaria and Sclerotiniaceae_unidentified in patients with erosive OLP were observed compared to the healthy controls.Aspergillus was identified as an “OLP-associated” fungus because of its detection at a higher frequency than in the healthy controls.Second,the co-occurrence patterns of the salivary mycobiome–bacteriome demonstrated negative associations between specific fungal and bacterial taxa identified in the healthy controls,which diminished in the reticular OLP group and even became positive in the erosive OLP group.Moreover,the oral cavities of OLP patients were colonized by dysbiotic oral flora with lower ecological network complexity and decreased fungal–Firmicutes and increased fungal–Bacteroidetes sub-networks.Third,several keystone fungal genera (Bovista,Erysiphe,Psathyrella,etc.) demonstrated significant correlations with clinical scores and IL-17 levels.Thus,we established that fungal dysbiosis is associated with the aggravation of OLP.Fungal dysbiosis could alter the salivary bacteriome or may reflect a direct effect of host immunity,which participates in OLP pathogenesis. | Yan Li Kun Wang Bo Zhang Qichao Tu Yufei Yao Bomiao Cui Biao Ren Jinzhi He Xin Shen Joy D.Van Nostrand Jizhong Zhou Wenyuan Shi Liying Xiao Changqing Lu Xuedong Zhou | 2019 | International Journal of Oral Science2019，11，3： | 8 |
| 11 | 早期断奶仔猪的生物学应激显示文摘当仔猪断奶后离开其母猪时,会遭遇到生物学应激,如生理的、环境的和社会的挑战。断奶过程是猪一生中最具应激性的事件,会造成肠道和免疫系统的机能障碍,结果导致猪健康状况、生长性能和采食量的降低,特别是断奶后首周内。饲养系统、营养、健康和管理技术的改进已被用来将断奶应激的一些不利影响降至最小程度,但是更好地了解应激的生物学作用,为改进能够克服断奶应激的策略所需要的。本综述的重点,是简要地描述与断奶有关的生物学应激是如何通过影响肠道形态、结构、生理和肠道免疫反应,进而可能会影响生产效率,如生长、采食量、发病率和死亡率。 | 陈建康 Joy M Campbell Joe D Crenshaw Javier Polo | 2014 | 国外畜牧学(猪与禽)2014，0，S1： | 8 |
| 12 | Lubiprostone vs Senna in postoperative orthopedic surgery patients with opioid-induced constipation:A double-blind,active-comparator trial显示文摘AIM:To investigate the efficacy of lubiprostone compared to Senna on bowel symptoms and constipation in post-operative orthopedic patients treated with opioids.METHODS:In this double blind,randomized,active comparator trial,adults who required opioids for analgesia following orthopedic procedures and who were admitted in inpatient rehabilitation were randomized following baseline assessments to lubiprostone(Amitza),orally twice aday or Senna(generic)two capsules administered daily for six days.Subjects were assessed using the patient assessment of constipation(PAC)-symptoms(PAC-SYM)and the PAC-quality of life(PAC-QOL)scales measured at baseline and Day 7;Subjects were assessed daily for secondary measures included the Bristol stool scale bowel consistency,specific bowel symptom score(Nausea,cramping,straining,completeness,abdominal pain,time per lavatory attempt,assistance needed),adverse events and rescue medications required.Function was measured using the functional independence measure(FIM)at admission and discharge;length of stay(LOS)and missed treatments due to gastrointestinal symptoms were also assessed.RESULTS:64 adults were enrolled;56 participants(28 in each group)had baseline and follow up measures and were included in the intention to treat(ITT)analyses.43 participants completed the study,21 in the active lubiprostone and 22 in the active Senna group.The mean age of the participants was 71.5years(SD=11.4 years,range:28-96 years).In the ITT analyses,participants showed significant improvement in bowel symptoms as measured by the PACSYM(mean±SD,-0.28±0.60,range:-1-2.33)and PAC-QOL(mean±SD,0.33±0.81,range:-1.5-2.0)over time,but there were no significant differences between the lubiprostone and Senna groups in mean change in the PAC-SYM(-0.20±0.60 vs-0.36±0.61,P=0.61 respectively)or the PAC-QOL(0.29±0.76 vs0.37±0.87,P=0.61 respectively).The mean change in each bowel symptom also did not significantly differ between treatment groups on ITT analyses,except for completeness of bowel movement,with the Senna group showing greater negative mean change in bowel movement completeness(-0.56±1.01 vs-2.00±1.41,P=0.03)and for reduction of abdominal pain,favoring Senna(-0.14±0.73 vs-0.73±1.08,P=0.04).Fifteen(75%)participants in the lubiprostone and in the Senna group requested rescue treatments.Participants made significant functional improvement from admission to discharge over a median LOS of 12 d,with a mean FIM change of 29.13±13.58 and no significant between group differences(27.0±9.2 vs 31.5±16.6,P=0.27).CONCLUSION:Both lubiprostone and Senna improved constipation-related symptoms and QOL in opioid-induced constipation,with no significant between-group differences. | Christina M Marciniak Santiago Toledo Jungwhalia Lee Michael Jesselson Jillian Bateman Benjamin Grover Joy Tierny | 2014 | World Journal of Gastroenterology2014，20，43： | 7 |
| 13 | 不同玉米大豆间作行比对大豆光合特性及产量的影响显示文摘为研究适合东北黑土区玉米大豆间作行比,促进玉米大豆间作模式推广应用,本研究以玉米大豆间作系统为主要研究对象,设置玉米单作(M)、大豆单作(S)、玉米大豆行比2∶4间作(M2S4)、玉米大豆行比4∶4间作(M4S4)和玉米大豆行比4∶6间作(M4S6)处理,分析不同间作行比对大豆株高及干物质积累、光合特性相关指标、产量及经济产值分析的影响。结果表明:间作显著增加了大豆株高,降低了干物质量,同时,随着玉米条带数的降低及大豆条带数的增加,光合参数Gs和Tr与单作处理之间呈显著性差异,而Ci则呈现相反的变化趋势。4∶4间作行比(M4S4)处理具有较好的间作优势,土地当量比为1.26,在考虑了政府补贴后,间作复合经济产值显著高于各单作处理,其中,M4S4处理最具有增收潜力。综上,M4S4模式为东北黑土区最佳玉米大豆间作配置方式。 | 曹曼君 王婧瑜 崔悦 Deborah Joy Blessing 李双 陈喜凤 谷岩 | 2023 | 大豆科学2023，42，1： | 7 |
| 14 | 863 genomes reveal the origin and domestication of chicken显示文摘Despite the substantial role that chickens have played in human societies across the world,both the geographic and temporal origins of their domestication remain controversial.To address this issue,we analyzed 863 genomes from a worldwide sampling of chickens and representatives of all four species of wild jungle fowl and each of the five subspecies of red jungle fowl(RJF).Our study suggests that domestic chickens were initially derived from the RJF subspecies Gallus gallus spadiceus whose present-day distribution is predominantly in southwestern China,northern Thailand and Myanmar.Following their domestication,chickens were translocated across Southeast and South Asia where they interbred locally with both RJF subspecies and other jungle fowl species.In addition,our results show that the White Leghorn chicken breed possesses a mosaic of divergent ancestries inherited from other subspecies of RJF.Despite the strong episodic gene flow from geographically divergent lineages of jungle fowls,our analyses show that domestic chickens undergo genetic adaptations that underlie their unique behavioral,morphological and reproductive traits.Our study provides novel insights into the evolutionary history of domestic chickens and a valuable resource to facilitate ongoing genetic and functional investigations of the world's most numerous domestic animal. | Ming-Shan Wang Mukesh Thakur Min-Sheng Peng Yu Jiang Laurent Alain Francois Frantz Ming Li Jin-Jin Zhang Sheng Wang Joris Peters Newton Otieno Otecko Chatmongkon Suwannapoom Xing Guo Zhu-Qing Zheng Ali Esmailizadeh Nalini Yasoda Hirimuthugoda Hidayat Ashari Sri Suladari Moch Syamsul Arifin Zein Szilvia Kusza Saeed Sohrabi Hamed Kharrati-Koopaee Quan-Kuan Shen Lin Zeng Min-Min Yang Ya-Jiang Wu Xing-Yan Yang Xue-Mei Lu Xin-Zheng Jia Qing-Hua Nie Susan Joy Lamont Emiliano Lasagna Simone Ceccobelli Humpita Gamaralalage Thilini Nisanka Gunwardana Thilina Madusanka Senasige Shao-Hong Feng Jing-Fang Si Hao Zhang Jie-Qiong Jin Ming-Li Li Yan-Hu Liu Hong-Man Chen Cheng Ma Shan-Shan Dai Abul Kashem Fazlul Haque Bhuiyan Muhammad Sajjad Khan Gamamada Liyanage Lalanie Pradeepa Silva Thi-Thuy Le Okeyo Ally Mwai Mohamed Nawaz Mohamed Ibrahim Megan Supple Beth Shapiro Olivier Hanotte Guojie Zhang Greger Larson Jian-Lin Han Dong-Dong Wu Ya-Ping Zhang | 2020 | Cell Research2020，30，8： | 7 |
| 15 | 胚乳特异sbeⅡa启动子在小麦中的评价及特性分析显示文摘淀粉的合成是一个复杂的生化过程。sbeⅡa基因是淀粉合成过程中的关键酶基因之一,在小麦籽粒成熟过程中对淀粉,尤其对支链淀粉的生物合成调控起重要作用。为了解sbeⅡa基因的调控机理及其胚乳特异表达特性,利用APCR方法克隆了sbeⅡa启动子3094 bp片段,并对该片段进行测序,同时利用GUS瞬间表达体系对sbeⅡa启动子序列进行不同片段的功能缺失研究。研究结果表明,3094 bp序列(存在于sbe.g融合体中)具有稳定的启动子活性,而5'或3'缺失体的启动子活性明显降低。在sbeⅡa启动子中间缺失体中,一些缺失体仅有微弱活性,但sbe.e在缺失-1579—1210 bp片段情况下,却具有比sbe.g(含启动子全长序列)还要高的启动子活性,研究初步证明,一些固定序列(motifs),如-300 bp因子、G盒以及醇溶蛋白盒(Prolamin box)等作为正调控因子在决定启动子胚乳特异表达模式中是必需的,而且在-1579—1210 bp片段中也存在一些负调控因子或固定序列。在瞬间表达检测体系中,小麦胚乳组织年龄对检测结果有着重要影响。 | 苗红梅 Fleming Joy E 吕德彬 韩锦峰 | 2004 | 科学通报2004，49，5： | 7 |
| 16 | Mitochondrial dysfunction activates the AMPK signaling and autophagy to promote cell survival显示文摘Autophagy is a cellular self-eating process essential for stress response and maintainingtissue homeostasis by lysosomal degradation of unwanted or damaged proteins and organelles.Here, we show that cells with defective mitochondria induce autophagy to promotecell survival through activating the AMPK pathway. Loss of mitochondrial complex III proteincytochrome b activates the AMPK signaling and induced autophagy. Inhibiting mitochondria energeticsby mitochondria-targeted agents activates the AMPK signaling and induced autophagy.Genetic inhibition of AMPK inhibits autophagy induction in cells with defective mitochondria,while genetic inhibition of autophagy has no effect on AMPK activation. Mitochondria dysfunctionhas no effect of DNA repair of UV-induced DNA damage. However, mitochondria dysfunctionsensitizes cells to apoptosis induced by UV radiation. Genetic inhibition of autophagy orAMPK sensitized cells to apoptosis in cells with defective mitochondria. Our results demonstratethat AMPK and autophagy senses mitochondria dysfunction and serves as a mechanismfor survival. Our findings may provide new insights into the interplay between mitochondriafunction and autophagy process in maintaining tissue homeostasis, and suggest that this interactionmay play important roles in diseases such as cancer and neurodegeneration. | Baozhong Zhao Lei Qiang Joy Joseph Balaraman Kalyanaraman Benoit Viollet Yu-Ying He | 2016 | Genes & Diseases2016，3，1： | 6 |
| 17 | 水稻组织培养中Tos17诱发体细胞无性系变异纯合体筛选技术初探显示文摘利用PCR技术对从日本引进的Tos17插入水稻极限糊精酶基因变异体后代进行了插入位点鉴定和纯合体筛选分析。结果表明,利用Tos17末端2 6bpDNA序列和插入位点上下游各2 0 0bp和30 0bp处的目标基因序列片段设计引物,可以有效地进行Tos17插入突变纯合体筛选,为突变体的进一步利用提供了分子依据。 | 黄冰艳 吉万全 海燕 康明辉 侯大虎 Fleming Joy 李忠谊 Rahman Sadequr | 2005 | 河南农业科学2005，34，6： | 6 |
| 18 | The crystal structure of the non-liganded 14-3-3σ protein: insights into determinants of isoform specific ligand binding and dimerization显示文摘Seven different, but highly conserved 14-3-3 proteins are involved in diverse signaling pathways in human cells. It isunclear how the 14-3-3σ isoform, a transcriptional target of p53, exerts its inhibitory effect on the cell cycle in thepresence of other 14-3-3 isoforms, which are constitutively expressed at high levels. In order to identify structuraldifferences between the 14-3-3 isoforms, we solved the crystal structure of the human 14-3-3σ protein at a resolutionof 2.8 ? and compared it to the known structures of 14-3-3ζ and 14-3-3τ. The global architecture of the 14-3-3σ foldis similar to the previously determined structures of 14-3-3ζ and 14-3-3τ: two 14-3-3σ molecules form a cup-shapeddimer. Significant differences between these 14-3-3 isoforms were detected adjacent to the amphipathic groove, whichmediates the binding to phosphorylated consensus motifs in 14-3-3-ligands. Another specificity determining region islocalized between amino-acids 203 to 215. These differences presumably select for the interaction with specific ligands,which may explain the different biological functions of the respective 14-3-3 isoforms. Furthermore, the two 14-3-3σmolecules forming a dimer differ by the spatial position of the ninth helix, which is shifted to the inside of the ligandinteraction surface, thus indicating adaptability of this part of the molecule. In addition, 5 non-conserved residues arelocated at the interface between two 14-3-3σ proteins forming a dimer and represent candidate determinants of homo-and hetero-dimerization specificity. The structural differences among the 14-3-3 isoforms described here presumablycontribute to isoform-specific interactions and functions. | Anne BENZINGER Grzegorz M. POPOWICZ Joma K. JOY Sudipta MAJUMDAR Tad A. HOLAK Heiko HERMEKING | 2005 | Cell Research2005，15，4： | 6 |
| 19 | 纸条检测法对第85窟壁画空鼓灌浆脱盐效果的评估显示文摘实验得出不损伤壁画的纸条检测法与壁画表面盐分含量的关系,并以此为根据,采用纸条法测定壁画表面盐分的含量状态,评价壁画空鼓灌浆后脱盐的效果。结果表明壁画空鼓灌浆所带入的水分激活了地仗中的盐分,使盐分运移并大量地富集到壁画的表面。保护笔的脱盐效果十分显著,纸条法测得保护笔脱盐后Cl-浓度降低约10个ppm,相当于保护笔脱盐可以减少了壁画表面地仗中所含1.6%的Cl-的量,即可以去除掉未灌浆区域壁画表面盐分的80%。实验证明纸条法可以应用于壁画脱盐效果的检验。 | 陈港泉 Michael Schilling 李燕飞 Neville Agnew Joy Keeney Stephen Rickerby 于宗仁 Lisa Shekede | 2007 | 敦煌研究2007，，5： | 5 |
| 20 | Biology and control of the fruit borer, Conopomorpha sinensis Bradley on litchi (Litchi chinensis Sonn.) in northern Thailand显示文摘水果和叶由水果 borer, Conopomorpha sinensis 幼虫和 C 的自然地发生的人口荔枝(荔枝 chinensis ) 射击群袭。sinensis parasitization 率在高度在北泰国在荔枝果园被决定(ca。1 400 m ASL ) 并且低(ca。800 m ASL ) 举起与不同, non-over-lapping fruiting 季节。另外,喷洒杆菌 thuringiensis var 的应用。aizawai,自然复合 spinosad 和化学农药 imidacloprid 在这块地里被进行在 C 上测试这些代理人的效果。sinensis 水果群袭率。水果群袭率和水果生长,在一个低举起果园学习了,是 sigmoidal 并且显示出一道高度重要的正相关(Pp “ 0.01 ) 。在低举起果园的叶射击群袭率在 fruiting 季节期间减少了(前进直到 5 月) ,但是在高举起果园增加了,在没有水果在时间的一样的时期以内是在场的的地方。一起,这些结果显示那 C 的女性。sinensis 清楚地为产卵离子喜欢水果甚于射击。如果没有水果是可得到的,它在此期间是在高举起果园的盒子,他们被抑制在射击上生他们的鸡蛋。C 的 parasitization 率。在未经治疗的水果的 sinensis 幼虫是 54.3% 。parasitoid 种类是 Chelonus chailini (90.8%) 和 Phanerotoma sp。(9.2%) 。在叶射击, C。sinensis 被发现被 Phanerotoma sp 寄生于。并且 Apanteles briareus。不同活跃代理人的应用没在 C 上有重要效果。与未经治疗的控制相比的 sinensis 水果群袭。除幼虫的神秘生活以外,这被事实最可能解释那 C。sinensis 在 fruiting 季节期间有几代。因为记录的高 parasitization 率,一种选择和更有效的控制比杀虫剂的申请可能是 parasitoids 的有增加力的版本测量。 | MARIE JOY SCHULTE KONRAD MARTIN JOACHIM SAUERBORN | 2007 | Insect Science2007，14，6： | 5 |
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