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| 1 | 14-3-3 proteins—an update显示文摘14-3-3 is a highly conserved acidic protein family, composed of seven isoforms in mammals. 14-3-3 protein caninteract with over 200 target proteins by phosphoserine-dependent and phosphoserine-independent manners. Little isknown about the consequences of these interactions, and thus are the subjects of ongoing studies. 14-3-3 controls cellcycle, cell growth, differentiation, survival, apoptosis, migration and spreading. Recent studies have revealed newmechanisms and new functions of 14-3-3, giving us more insights on this fascinating and complex family of proteins.Of all the seven isoforms, 14-3-3σ seems to be directly involved in human cancer. 14-3-3σ itself is subject to regulationby p53 upon DNA damage and by epigenetic deregulation. Gene silencing of 14-3-3σ by CpG methylation has beenfound in many human cancer types. This suggests that therapy-targeting 14-3-3σ may be beneficial for future cancertreatment. | Paulette MHAWECH | 2005 | Cell Research2005，15，4： | 32 |
| 2 | The role of epigenetic inactivation of 14-3-3σ in human cancer显示文摘Cancer cells show characteristic alterations in DNA methylation patterns. Aberrant CpG methylation of specificpromoters results in inactivation of tumor suppressor genes and therefore plays an important role in carcinogenesis. Thep53-regulated gene 14-3-3σ undergoes frequent epigenetic silencing in several types of cancer, including carcinoma ofthe breast, prostate, and skin, suggesting that the loss of 14-3-3σ expression may be causally involved in tumor progression.Functional studies demonstrated that 14-3-3σ is involved in cell-cycle control and prevents the accumulation of chro-mosomal damage. The recent identification of novel 14-3-3σ-associated proteins by a targeted proteomics approachimplies that 14-3-3σ regulates diverse cellular processes, which may become deregulated after silencing of 14-3-3σexpression in cancer cells. | Dmitri LODYGIN Heiko HERMEKING | 2005 | Cell Research2005，15，4： | 8 |
| 3 | 14-3-3σ蛋白在人类恶性肿瘤中的研究进展显示文摘14-3-3蛋白是高度保守的可溶性酸性蛋白家族,近年的研究表明,它们在细胞有丝分裂、生长、分化、增殖和死亡等过程中起重要的调控作用。其中14-3-3σ主要存在于上皮细胞,具有组织特异性,可以与细胞内多种信号蛋白相互作用,促进DNA损伤后的细胞周期G2期阻滞,调控细胞的生长、分化和增殖等多种生物学功能,在肿瘤发生发展过程中表达异常,可能由p53突变或启动子CpG岛超甲基化引起,与人类恶性肿瘤的发生发展及预后密切相关。该文作者仅就14-3-3σ蛋白在人类恶性肿瘤中的研究进展作一综述。 | 潘敏鸿 周晓军 | 2007 | 医学研究生学报2007，20，4： | 5 |
| 4 | Integrate Omics Data and Molecular Dynamics Simulations toward Better Understanding of Human 14-3-3 Interactomes and Better Drugs for Cancer Therapy显示文摘The 14-3-3 protein family is among the most extensively studied, yet still largely mysterious protein families in mammals to date. As they are well recognized for their roles in apoptosis, cell cycle regulation, and proliferation in healthy cells, aberrant 14-3-3 expression has unsurprisingly emerged as instrumental in the development of many cancers and in prognosis. Interestingly, while the seven known 14-3-3isoforms in humans have many similar functions across cell types, evidence of isoform-specific functions and localization has been observed in both healthy and diseased cells. The strikingly high similarity among 14-3-3 isoforms has made it difficult to delineate isoform-specific functions and for isoform-specific targeting. Here, we review our knowledge of 14-3-3 interactome(s) generated by highthroughput techniques, bioinformatics, structural genomics and chemical genomics and point out that integrating the information with molecular dynamics(MD) simulations may bring us new opportunity to the design of isoform-specific inhibitors, which can not only be used as powerful research tools for delineating distinct interactomes of individual 14-3-3 isoforms, but also can serve as potential new anti-cancer drugs that selectively target aberrant 14-3-3 isoform. | Jo Anne J.Babula Jing-Yuan Liu | 2015 | Journal of Genetics and Genomics2015，42，10： | 1 |
| 5 | PRAS40与14-3-3蛋白各亚型相互作用的酵母双杂交系统检测显示文摘PRAS40是近几年新发现的Akt作用底物,14-3-3结合蛋白。为确定PRAS40与14-3-3蛋白7种亚基间相互作用关系,利用gateway方法构建用于酵母双杂交系统的诱饵质粒pEG-PRAS40及转录激活质粒pJG-PRAS40,将PRAS40和14-3-3各亚型质粒分别作为诱饵蛋白质粒及转录激活质粒共转化酵母细胞EGY48,通过氨基酸营养缺陷生长实验及β-半乳糖苷酶显色反应分析两种蛋白相互作用程度。酶切鉴定证实成功地构建了pEG-PRAS40和pJG-PRAS40质粒,酵母双杂交实验结果显示PRAS40可以和14-3-3亚型tau,beta,zeta及epsilon相结合,epsilon较强,beta和zeta次之,tau较弱。此结果将为深入研究PRAS40与14-3-3蛋白生物学功能及发现药物靶标奠定基础。 | 刘康武 黄蓓 谭阳 吴东明 | 2007 | 生物工程学报2007，23，4： | 0 |
| 6 | 14-3-3σ蛋白表达对恶性肿瘤诊断和治疗的意义显示文摘 | 敖梅红 李隆玉 | 2006 | 实用癌症杂志2006，21，5： | 0 |
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