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7篇 您的检索式：作者名="John J Harding"

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		题名	作者	年代	出处	被引量
	1	Identification of the preferentially targeted proteins by carbamylation during whole lens incubation by using radio-labelled potassium cyanate and mass spectrometry显示文摘AIM:To attempt to identify the primary targets of carbamylation in bovine lenses incubated under physiological condition. METHODS:Fresh intact bovine lenses were incubated with [¹⁴C]-labelled potassium cyanate for seven days.The water-soluble proteins(WSP)of both cortex and nucleus lens were isolated by size-exclusion chromatography on a Sephacryl S-300HR column.The higher radioactive fractions were pooled and freeze-dried,and separated further by loading on an Affi Blue column to separate some enzymes.In addition,WSP from cortex was separated directly by affinity chromatography.The most reactive fractions with higher radioactivity from[¹⁴C]-cyanate were further analysed by SDS-gels and mass spectrometry. RESULTS:The majority of protein incorporating[¹⁴C]-labelled potassium cyanate was in the water-soluble fractions,and much more in cortex than in nucleus.Chromatography results demonstrated that the major incorporated[¹⁴C]-carbamylated crystallins were fractions corresponding toα-crystallin,β-crystallin andξ-crystallin in cortex,butβ-crystallin andγ-crystallin in nucleus.The SDS gels showed that bound fractions of cortex crystallins after Affi-Gel Blue separation were abundant 20 and 35kDa proteins.However,the bound fractions of nucleus crystallins mainly showed 20kDa proteins. Mass spectrometry analysis of these higher radioactivity fractions and a database search revealed that the proteins originated from bovineα-crystallin A and B chains andξ-crystallin in cortex;βA1 andαB-crystallins with a littleγB-crystallin in nucleus respectively.Further analysis suggested the location of this carbamylation ofαB-crystallin in nucleus to be at Lys 92 and 103. CONCLUSION:α-andξ-crystallin from cortex can be preferentially targeted by carbamylation during whole lens incubations.Carbamylation of these crystallins at the earlier stage may lead to further unfolding and misfolding of lens proteins,leading to aggregation of crystallins and eventually to cataract formation.	John J Harding	2010	International Journal of Ophthalmology(English edition)2010，3，2：	3
	2	REM sleep deprivation-induced deficits in the latency-to-peak induction and maintenance of long-term potentiation within the CA1 region of the hippocampus显示文摘	Christopher J Davis Joseph W Harding John W Wright		0，，02：	1
	3	PTEN can inhebit in vitro organotypic and in vivo orthotopic invasion of human bladder cancer cells even in the absence of its lipid phosphatase activity 显示文摘	John J Gildea Mikael Herlevsen Michael A Harding	2004	Oncogene2004，23，40：	1
	4	PTEN can inhibit in vitro organotypic and in vivo orthotopic of human bladder cancer cells even in the absence of its lipid phosphatase activity 显示文摘	John J Gildea Mikael Herlevsen Michael A Harding	2004	Oncogene2004，23，40：	1
	5	Cardiovascular effects of tumour necrosis factor α antagonism in patients with acute myocardial infarction: a first in human study显示文摘	Gareth J Padfield Jehangir N Din Elena Koushiappi Nicholas L Mills Simon D Robinson Nicholas Le May Cruden Andrew John Lucking Stanley Chia Scott A Harding David E Newby	2013	Heart2013，，18：	1
	6	Hybrid shortest path and ray bending method for travel and raypath calculations显示文摘	Alistair J Harding John A Orcutt	2001	Geophysics2001，66，2：	1
	7	Cardiovascular effects of tumour necrosis factor α antagonism in patients with acute myocardial infarction: a first in human study显示文摘	Gareth J Padfield Jehangir N Din Elena Koushiappi Nicholas L Mills Simon D Robinson Nicholas Le May Cruden Andrew John Lucking Stanley Chia Scott A Harding David E Newby	2013	Heart2013，，18：	1

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