|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 精液中硒含量与精子质量和精子DNA氧化损伤的关系显示文摘目的 探讨血清和精液中硒浓度与精子质量和精子DNA氧化损伤的关系。方法 用原子吸收光谱检测血清和精液中硒浓度 ,按WHO规范方法检测精液量、精子数、每次射出精子总数、活力和活动精子百分率及精子畸形率 ,用高压液相色谱 光电检测系统和高压液相色谱 紫外线检测器同时检测人精子DNA 8 羟基脱氧核糖鸟苷 (8 OHdG)和脱氧核糖鸟苷 (dG)水平。结果 已孕组精液硒平均浓度 (0 74μmol/L)明显高于不孕组 (0 5 6 μmol/L) ,而两组间血清硒平均浓度差异无显著性。已孕组精液量、精子数和每次射出精子总数的平均值 (3 91ml、46 4× 10 9/L和 16 8 0× 10 6)明显高于不孕组 (2 79ml、36 5× 10 9/L和 10 2 0× 10 6) ,两组间精子活力、活动精子百分率和精子畸形率的平均值差异无显著性。精液硒浓度与精子数、每次射出精子总数、精子活力、活动精子百分率之间呈明显的正相关 ,而血清硒浓度与精子质量均无明显相关关系。精子DNA 8 OHdG水平与精液硒浓度呈明显负相关 ,而与血清硒浓度无明显相关关系。结论 精液硒浓度与人类精子质量和精子DNA 8 OHdG有关 。 | 徐德祥 Ong Choonnam Shen Hanming Chua Laiha Chia Seneng | 2001 | 中华预防医学杂志2001，35，6： | 16 |
| 2 | 欧洲越桔花青素提取物对小鼠耐常压缺氧能力的影响显示文摘目的研究欧洲越桔花青素提取物(Myrtillus EX)对常压缺氧负荷小鼠抗缺氧能力的影响。方法采用HPLC/HPLC-MS分析Myrtillus EX中的不同花青素类成分。通过常压缺氧负荷小鼠模型观察Myrtillus EX对小鼠存活时间和耗氧量的影响,利用ORAC法测定血浆、大脑皮质及肝组织匀浆中的抗氧化能力指数,Griess化学法测定NO含量,以及TBARS法测定血浆、大脑皮质及肝组织匀浆中的MDA含量。结果Myrtillus EX可以明显提高常压缺氧负荷小鼠的耐缺氧能力,有效降低血浆、大脑皮质及肝组织匀浆中的MDA含量,并调节血浆、大脑皮质及肝组织匀浆中的NO含量。结论Myrtillus EX对常压缺氧负荷小鼠显示一定的保护作用,其机制可能与其自由基清除能力和抑制脂质过氧化作用有关。 | 宝丽 姚新生 吕艳青 李满妹 Chia ChewSern Tsi Daneil 永井元 栗原博 | 2007 | 中国药理学通报2007，23，11： | 15 |
| 3 | 黔金丝猴的行为谱及PAE编码系统显示文摘2009年10月~2014年4月,在贵州梵净山国家级自然保护区和北京动物园,采用焦点动物取样法和随意取样法,观察记录了野外和圈养黔金丝猴Rhinopithecus brelichi行为发生的过程及环境特征。按照以"姿势-动作-环境"(posture-act-environment,PAE)为轴心,以行为的生态功能为依据的PAE编码系统,对黔金丝猴的行为进行分类和系统编码并构建PAE行为谱。分辨并记录了黔金丝猴的16种姿势,107种动作及163种行为,将记录到的行为分别划归于摄食、排遗、调温、交配、分娩、育幼、亲密、作威、冲突、通讯、聚群、休息、运动和杂类等14个类别中;区别了各种行为在雄性、雌性、亚成体、幼猴和婴猴之间的相对发生频次以及发生季节。与川金丝猴R.roxellana和滇金丝猴R.bieti相比,黔金丝猴在个体行为和社会行为方面均有所不同,这可能与三者生活环境差异有关。 | 崔多英 牛克锋 Tan Chia Luen Yang Mouyu 张媛媛 张金国 杨业勤 | 2014 | 四川动物2014，33，6： | 14 |
| 4 | miRNA studies in in vitro and in vivo activated hepatic stellate cells显示文摘AIM: To understand which and how different miRNAs are implicated in the process of hepatic stellate cell (HSC) activation. METHODS: We used microarrays to examine the differential expression of miRNAs during in vitro activation of primary HSCs (pHSCs). The transcriptome changes upon stable transfection of rno-miR-146a into an HSC cell line were studied using cDNA microarrays. Selected differentially regulated miRNAs were investigated by quantitative real-time polymerase chain reaction during in vivo HSC activation. The effect of miRNA mimics and inhibitor on the in vitro activation of pHSCs was also evaluated.RESULTS: We found that 16 miRNAs were upregulated and 26 were downregulated significantly in 10-d in vitro activated pHSCs in comparison to quiescent pHSCs. Overexpression of rno-miR-146a was characterized by marked upregulation of tissue inhibitor of metalloproteinase-3, which is implicated in the regulation of tumor necrosis factor-α activity. Differences in the regulation of selected miRNAs were observed comparing in vitro and in vivo HSC activation. Treatment with miR-26a and 29a mimics, and miR-214 inhibitor during in vitro activation of pHSCs induced significant downregulation of collagen type Ⅰ transcription. CONCLUSION: Our results emphasize the different regulation of miRNAs in in vitro and in vivo activated pHSCs. We also showed that miR-26a, 29a and 214 are involved in the regulation of collagen type I mRNA. | Gunter Maubach Michelle Chin Chia Lim Henry Yang | 2011 | World Journal of Gastroenterology2011，17，22： | 12 |
| 5 | Macrophage migration inhibitory factor stimulated by He/icobacter py/oriincreases proliferation of gastric epithelial cells显示文摘AIM: Helicobacter pylori (H pylori) is associated with increased gastric inflammatory and epithelial expression of macrophage migration inhibitory factor (MIF) and gastric epithelial cell proliferation. This study aimed at determining whether H pylori directly stimulates release of MIF in monocytes, whether the cay pathogenicity island (PAI) is involved for this function, and whether MIF stimulated by H pylori increases gastric epithelial cell proliferation in vitro.METHODS: A cytotoxic wild-type H pylori strain (TN2),its three isogenic mutants (TN2△cag, TN2△cagA and TN2△cagE) were co-cultured with cells of a human monocyte cell line, THP-1, for 24 h at different organism/cell ratios. MIF in the supernatants was measured by an ELISA. Cells of a human gastric cancer cell line, MKN45,were then co-cultured with the supernatants, with and without monoclonal anti-MIF antibody for 24 h. The cells were further incubated for 12 h after addition of 3H-thymidine, and the levels of incorporation of 3H-thymidine were measured with a liquid scintillation counter.RESULTS: The wild-type strain and the isogenic mutants,TN2△cagA and TN2△cagE, increased MIF release at organism/cell ratios of 200/1 and 400/1, but not at the ratios of 50/1 and 100/1. However, the mutant TN2△cag did not increase the release of MIF at any of the four ratios.3H-thymidine readings for MKN-45 cells were significantly increased with supernatants derived from the wild-type strain and the mutants TN2△cagA and TN2△cagE, but not from the mutant TN2△cag. Moreover, in the presence of monoclonal anti-MIF antibody, the stimulatory effects of the wild-type strain on cell proliferation disappeared.CONCLUSION: H pylori stimulates MIF release in monocytes, likely through its cag PAI, but not related to cagA or cagE. H pylori-stimulated monocyte culture supernatant increases gastric cell proliferation, which is blocked by anti-MIF antibody, suggesting that MIF plays an important role in H pylori-induced gastric epithelial cell proliferation. | Harry Hua-Xiang Xia Shiu Kum Lam Annie O.O.Chan Marie Chia Mi Lin Hsiang Fu Kung Keiji Ogura Douglas E.Berg Benjamin Chun-Yu Wong | 2005 | World Journal of Gastroenterology2005，11，13： | 12 |
| 6 | 中国麻阳河国家级自然保护区黑叶猴种群数量估计与分布显示文摘2012年10月至2015年5月,对中国贵州省麻阳河国家级自然保护区黑叶猴(Trachypithecus francoisi)的种群数量和分布进行了调查研究。本次调查采用了直接观察猴群计数、夜宿地观察、问卷调查和访问、未出版资料及文献补充4种调查方法。结果表明,麻阳河国家级自然保护区及周边共有黑叶猴72群(含保护区外2群)。基于直接计数的25个猴群,计算猴群平均大小为(7.7±2.9)只,并以此计算保护区及周边黑叶猴数量为554只。其中,凉桥片区共有41群,315只;龚溪口片区共有16群,123只;务川片区共有15群,116只。调查发现保护区黑叶猴的分布基本以麻阳河、洪渡河及其支流兰子河为中轴,以箱状河谷的峭壁上和河岸附近的植被区作为主要活动范围。67群猴群发现点位于离河流中轴线缓冲距1 500 m区域以内,占总群数的93.1%。因此,为了确保该物种的生存以及缓解当地人猴冲突,建议重点加强对河流两岸栖息地的保护管理和自然植被恢复,尤其在人口稠密区更为必要。 | 牛克锋 肖志 王彬 杨德 Chia L Tan 张鹏 颜修刚 王华 余波 杨天友 樊均德 崔多英 邹启先 吴安康 魏鲁明 邹浩 Marco Gamba Cristina Giacoma 杨业勤 | 2016 | 动物学杂志2016，51，6： | 10 |
| 7 | Polymorphisms of MTHFR and susceptibility to oesophageal adenocarcinoma in a Caucasian United Kingdom population显示文摘AIM:To identify if methylene tetra-hydrofolatereductase(MTHFR)C677T polymorphisms are associated with oesophageal adenocarcnomas in a Caucasian population and to test whether folic acid and homocysteine levels are linked with cancer risk.METHODS:A case control study comprising of 58 non cancer and 48 cancer patients,MTHFR C667T genotyping was made and serum folate,homocysteine and vitamin B12 levels were made.Tumour stage,differentiation and survival was recorded.A P value of less than0.05 was taken to be significant.Theχ2 used to compare discrete variables and the Mantel-Cox was used to compare survival.A P value less than 0.05 was deemed to be significant.RESULTS:MTHFR polymorphisms is associated with an increased risk of several cancers.A link between MTHFR C677T polymorphisms and oesophageal squamous cell carcinoma and gastric cardia adenocarcinoma has been demonstrated in at risk Chinese populations.In a Western European population the role of the MTHFR gene has not previously been investigated in the setting of oesophageal adenocarcinoma.No association between folic acid levels and cancer patients was found.The unstable MTHFR 667 TT genotype occurred in 11%cancers and 7%controls,but statistical significance was not reached,homocysteine levels and folic acid levels were not affected,cancer patients with TT genotype displayed a trend for a shorter survival 7 mo vs 20 mo.Serum vitamin B12 levels were higher in the cancer group.The MTHFR 667 TT genotype is much lower than previous population studies.CONCLUSION:We conclude that serum folic acid and MTHFR polymorphisms are not associated with an increased risk of oesophageal adenocarcinoma,although cancers with unstable TT genotype may indicate a more aggressive disease course. | Richard Keld Manyi Thian Chia Hau Jamil Sajid Narveen Kumar Yeng Ang | 2014 | World Journal of Gastroenterology2014，20，34： | 8 |
| 8 | 人肺组织内源性显微荧光特性研究显示文摘采用新型显微荧光分光光度系统 ,实现激光诱导人肺组织内源性显微荧光光谱与图像的测量 ,获得不同组织层 (即上皮层 ,粘膜层和软骨层 )的荧光分布和荧光特性 ,为探索癌与正常组织自体荧光光谱特征差异的根源 。 | 郑蔚 黄志伟 谢树森 S.M.Krishnan Chia Teck Chee | 2001 | 中国激光2001，28，7： | 7 |
| 9 | Effects of Enriched Biochars Containing Magnetic Iron Nanoparticles on Mycorrhizal Colonisation,Plant Growth,Nutrient Uptake and Soil Quality Improvement显示文摘At present, there is little commercial sale of biochar, since farmers find they can not gain a return on their investment in this amendment in the first few years after its application, because of the high cost associated with large application rates. To overcome this constraint, development of artificially aged enriched biochar-mineral complexes(BMCs), having a higher mineral content, surface functionality, exchangeable cations, high concentration of magnetic iron(Fe) nanoparticles, and higher water-extractable organic compounds has been undertaken by a combined team of researchers and a commercial company. Two biochars produced under different pyrolysis conditions were activated with a phosphoric acid treatment. A mixture of clay, chicken litter, and minerals were added to the biochar, and then this composite was torrefied at either 180 or 220?C. In this study a pot experiment was carried out in glasshouse conditions to determine the effects of four different BMCs, with different formulations applied at rates of 100 and 200 kg ha-1, on the mycorrhizal colonisation, wheat growth and nutrient uptake, and soil quality improvement. It was found that the phosphorus(P) and nitrogen uptake in wheat shoots were significantly greater for a low application rate of BMCs(100 kg ha-1). The present formulation of BMC was effective in enhancing growth of wheat at low application rate(100 kg ha-1). The increase in growth appeared due to an increase in P uptake in the plants that could be partly attributed to an increase in mycorrhizal colonisation and partly due to the properties of the BMC. | Stephen JOSEPH Hossain M.ANAWAR Paul STORER Paul BLACKWELL Chee CHIA Yun LIN Paul MUNROE Scott DONNE Josip HORVAT Jianli WANG Zakaria M.SOLAIMAN | 2015 | Pedosphere2015，25，5： | 7 |
| 10 | Gastric peritoneal carcinomatosis-a retrospective review显示文摘AIM To characterize patients with gastric peritoneal carcinomatosis(PC) and their typical clinical and treatment course with palliative systemic chemotherapy as the current standard of care.METHODS We performed a retrospective electronic chart review of all patients with gastric adenocarcinoma with PC diagnosed at initial metastatic presentation between January 2010 and December 2014 in a single tertiary referral centre.RESULTS We studied a total of 271 patients with a median age of 63.8 years and median follow-up duration of 5.1 mo. The majority(n = 217, 80.1%) had the peritoneum as the only site of metastasis at initial presentation. Palliative systemic chemotherapy was eventually planned for 175(64.6%) of our patients at initial presentation, of which 171 were initiated on it. Choice of first-line regime was in accordance with the National Comprehensive Cancer Network Guidelines for Gastric Cancer Treatment. Thesepatients underwent a median of one line of chemotherapy, completing a median of six cycles in total. Chemotherapy disruption due to unplanned hospitalizations occurred in 114(66.7%), while cessation of chemotherapy occurred in 157(91.8%), with 42 cessations primarily attributable to PC-related complications. Patients who had initiation of systemic chemotherapy had a significantly better median overall survival than those who did not(10.9 mo vs 1.6 mo, P < 0.001). Of patients who had initiation of systemic chemotherapy, those who experienced any disruptions to chemotherapy due to unplanned hospitalizations had a significantly worse median overall survival compared to those who did not(8.7 mo vs 14.6 mo, P < 0.001).CONCLUSION Gastric PC carries a grim prognosis with a clinical course fraught with disease-related complications which may attenuate any survival benefit which palliative systemic chemotherapy may have to offer. As such, investigational use of regional therapies is warranted and required validation in patients with isolated PC to maximize their survival outcomes in the long run. | Hwee Leong Tan Claramae Shulyn Chia Grace Hwei Ching Tan Su Pin Choo David Wai-Meng Tai Clarinda Wei Ling Chua Matthew Chau Hsien Ng Khee Chee Soo Melissa Ching Ching Teo | 2017 | World Journal of Gastrointestinal Oncology2017，9，3： | 6 |
| 11 | High Levels of Hepatitis B Surface Antigen Increase Risk of Hepatocellular Carcinoma in Patients With Low HBV Load显示文摘 | Tai–Chung Tseng Chun–Jen Liu Hung–Chih Yang Tung–Hung Su Chia–Chi Wang Chi–Ling Chen Stephanie Fang–Tzu Kuo Chen–Hua Liu Pei–Jer Chen Ding–Shinn Chen Jia–Horng Kao | 2012 | Gastroenterology2012，，5： | 6 |
| 12 | 秦岭川金丝猴一周岁内个体的行为发育显示文摘2003年3月至2004年5月在陕西周至国家自然保护区的玉皇庙地区,采用目标动物取样法(Focal ani-mal sampling)对2003年出生的5只金丝猴个体行为发育进行了观察,应用全事件记录法(All-occurrences record-ing)进行数据的收集。结果表明23日龄婴儿首次从母亲怀内爬出在树枝上活动,68日龄开始在树上攀爬跳跃,5月龄主动采食树叶和啃投食区食物,6月龄跟随社群迁移。依据婴幼儿主要行为首次出现时间及其变化,把1周岁内个体发育分为5个时期,即完全依赖期、探索外部世界期、融入社群期、适应生存期和逐步独立期。1周岁内婴幼儿活动和休息的地方是母亲怀里、其它个体怀里、树枝上和地面上。随着发育,婴幼儿在这些地方停留的时间也在变化。1周岁内婴幼儿在母亲怀中的时间与年龄存在明显的负相关,在其它个体怀里的时间与年龄也呈显著负相关。虽然独自在树上的时间与年龄相关性不显著,但在地面上停留的时间与年龄呈正相关。 | 李银华 李保国 Chia L.TAN | 2005 | 动物学报2005，51，6： | 6 |
| 13 | Effect of Synthesis Technique and Carbonate Content on the Crystallinity and Morphology of Carbonated Hydroxyapatite显示文摘The syntheses of nanosized carbonated hydroxyapatite(CHA) were performed by comparing dropwise and direct pouring of acetone solution of Ca(N03)2-4H20 into mixture of(NH4)2HP04 and NH4HCO3 at room temperature controlled at pH 11.Direct pouring method was later applied to study the increment of carbonate content in syntheses.The as-synthesized powders were characterized by various characterization techniques.The crystallographic results of the produced powders were obtained from X-ray diffraction analysis,whilst the carbonate content in the produced powders was determined by the CHNS/O elemental analyzer.Fourier transform infrared analysis confirmed that the CHA powders formed were B-type.Field emission scanning electron microscopy revealed that the powders were highly agglomerated in nanosized range and hence energy filtered transmission electron microscopy was employed to show elongated particles which decreased with increasing carbonate content. | Chia Ching Kee Hanafi Ismail Ahmad Fauzi Mohd Noor | 2013 | Journal of Materials Science & Technology2013，29，8： | 5 |
| 14 | 秦岭川金丝猴的一次家庭雄性替代(英文)显示文摘家庭雄性替代已在亚洲叶猴的许多种类中有所报道。 2 0 0 3年 ,我们在一种亚洲叶猴———秦岭的川金丝猴 (Rhinopithecusroxellana)的一个单雄家庭中观察到一次完整的家庭雄性替代过程。此次替代发生于 4月 2 8日 - 5月 2日的五天时间内。在家庭雄性发生替代后的第六个月 ,该家庭中的婴猴消失。本文是首次对野生川金丝猴家庭雄性替代进行详细的报道。通过本次观察证明野生川金丝猴的确存在家庭雄性替代的现象。且像亚洲叶猴的许多种类一样 ,野生川金丝猴的家庭雄性替代同样发生于产仔季节和交配季节之间。 | 王慧平 Chia L.TAN 高云芳 李保国 | 2004 | 动物学报2004，50，5： | 4 |
| 15 | 分布在中国贵州梵净山的黔金丝猴显示文摘黔金丝猴(Rhinopithecus brelichi)又名灰金丝猴、白肩仰鼻猴、牛尾猴、线狨、果然兽,为国家Ⅰ级重点保护动物,是中国特有种,仅分布在贵州梵净山国家级自然保护区,野外种群数量700余只(杨业勤等2002,国家林业局2009)。梵净山位于贵州东北部,我国自西而东层层下降地势第二阶梯的云贵高原向第三阶梯湘西丘陵的过渡地区,地理位置特殊。该区是我国亚热带东部湿润区保存完整、原生性较强、垂直分异明显的森林生态系统, | 崔多英 牛克锋 Chia L. Tan Mouyu Yang 石磊 张维勇 张金国 杨业勤 | 2014 | 动物学杂志2014，49，5： | 4 |
| 16 | Serum Hepatitis B Surface Antigen Levels Predict Surface Antigen Loss in Hepatitis B e Antigen Seroconverters显示文摘 | Tai–Chung Tseng Chun–Jen Liu Tung–Hung Su Chia–Chi Wang Chi–Ling Chen Pei–Jer Chen Ding–Shinn Chen Jia–Horng Kao | 2011 | Gastroenterology2011，，2： | 4 |
| 17 | 废米糠制备炭纳米颗粒及其对金属离子的光学传感性能(英文)显示文摘以废米糠为原料,浓硫酸为脱水剂,通过炭化法制备出炭纳米颗粒(CNPs),探讨CNPs荧光发射特征、金属离子的淬灭效应以及作为Sn(II)离子传感材料应用。CNPs产率最佳条件为:硫酸浓度12 mol/L、加热温度120℃及恒温时间30 min。样品在水中强蓝光的最大发射波为439 nm。通过加入金属离子,使金属离子与CNPs表面间形成复合物而淬灭荧光。Sn(II)离子对CNPs荧光具有显著的淬灭效应。Sn(II)离子浓度对淬灭效应符合Stern-Volmer线性关系,Sn(II)离子为6.13 mmol/L。Sn(II)离子的检测限为18.7μmol/L。 | Peggy Zhen Zhen Ngu Stephanie Pei Phing Chia Jessica Fung Yee Fong Sing Muk Ng | 2016 | 新型炭材料2016，31，2： | 4 |
| 18 | Diagnosis and fine localization of deletion region in Wolf- Hirschhorn syndrome patients显示文摘 | JI Tao-yun David CHIA WANG Jing-min WU Ye LI Jie XIAO Jing JIANG Yu-wu | 2010 | Chinese Medical Journal2010，，13： | 4 |
| 19 | GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line显示文摘瞄准:GFAP 传统地被认为是为神经神经胶质的一个简历标记(主要星形细胞和 non-myelinating Schwann 房间) 。遗传上,一个 2.2-kb 人 GFAP 倡导者成功地被用来指向星形细胞在试管内和在活体内。更最近, GFAP 也为识别肝的星形细胞(HSC ) 作为几个制造者之一被建立。在这个工程,为指向 HSC 的一样的 2.2-kb 人 GFAP 倡导者的可能的申请被调查。方法:GFAP-lacZ transgene 是进各种各样的房间线(HSC, hepatocyte,和另外的 non-HSC 房间类型) 的 transfected。transgene 表示特性被贝它牛乳糖活动染色的 X 女郎决定。并且 transgene 的应答的海角与典型 pro-fibrotic cytokine TGF-beta1 被测试。内长的 GFAP 基因的表示被即时 RT-PCR 估计,提供为 transgene 表示的一本参考书。结果:结果第一次证明 2.2 kb hGFAP 倡导者不仅能够指导 HSC 特定的表示,而且对已知的 pro-fibrogenic cytokine TGF-beta1 作出回应由在在一个剂量依赖者和时间依赖者举止的规定上面,类似于内长的 GFAP。结论:在结论,这些调查结果为使用 GFAP 倡导者明确地为治疗学的目的操作 HSC 建议了新奇实用程序。 | Gunter Maubach Michelle Chin Chia Lim | 2006 | World Journal of Gastroenterology2006，12，5： | 4 |
| 20 | H elicobacter pylori infection associated with high H b A 1c and type 2 diabetes显示文摘 | Ming‐Chia Hsieh Sophie S. W. Wang Yi‐Ting Hsieh Fu‐Chen Kuo Maw‐Soan Soon Deng‐Chyang Wu | 2013 | Eur J Clin Invest2013，，9： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员