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| 1 | XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice显示文摘 | Chunlian Wang Xiaoping Zhang Yinglun Fan Ying Gao Qinlong Zhu Chongke Zheng Tengfei Qin Yanqiang Li Jinying Che Mingwei Zhang Bing Yang Yaoguang Liu Kaijun Zhao | 2015 | Molecular Plant2015，8，2： | 55 |
| 2 | Dendritic Cell-Derived Exosomes Stimulate Stronger CD8^+ CTL Responses and Antitumor Immunity than TVimor Cell-Derived Exosomes显示文摘从树枝状的细胞(DC ) 和肿瘤细胞导出的 Exosomes (EXO ) 被用来在动物模型并且在临床的试用刺激 antitumor 免疫者回答。然而,有不,一起, antitumor 免疫者回答的 stimulatory 效率的比较由这二支通常使用的 EXO 疫苗导致了。在这研究,我们选择了房间表示学习 EXO 的特征从一个 transfected EG7 肿瘤导出的显型 ovalbumin (卵) 和由流动 cytometry 的搏动卵的 DC。我们在在 EXO 的这二种类型之间的卵特定的有免疫力的回答的正式就职比较了 stimulatory 效果。我们发现卵搏动蛋白质的导出 DCovA 的 EXO (EXODC ) 能更高效地在 vivo 刺激天真的卵特定的 CD8+ T 房间增长和区别进细胞毒素的 T 淋巴细胞,并且比 EG7 肿瘤导致更有效的 antitumor 免疫导出房间的 EXO (EXOEG7 ) 。另外,我们阐明了在 EXO 的 stimulatory 效果被主人 DC 送到 T 房间回答的 EXO 疫苗的主人 DC 的重要角色。因此,导出 DC 的 EXO 可以在 antitumor 免疫的正式就职代表一支更有效的基于 EXO 的疫苗。 | Siguo Hao Ou Bai Jinying Yuan Mabood Qureshi Jim Xiang | 2006 | Cellular & Molecular Immunology2006，3，3： | 30 |
| 3 | Gene Network Analysis and Functional Studies of Senescence-associated Genes Reveal Novel Regulators of Arabidopsis Leaf Senescence显示文摘Plant leaf senescence has been recognized as the last phase of plant development, a highly ordered process regulated by genes known as senescence associated genes (SAGs). However, the function of most of SAGs in regulating leaf senescence as well as regulators of those functionally known SAGs are still unclear. We have previously developed a curated database of genes potentially associated with leaf senescence, the Leaf Senescence Database (LSD). In this study, we built gene networks to identify common regulators of leaf senescence in Arabidopsis thaliana using promoting or delaying senescence genes in LSD. Our results demonstrated that plant hormones cytokinin, auxin, nitric oxide as well as small molecules, such as Ca2+, delay leaf senescence. By contrast, ethylene, ABA, SA and JA as well as small molecules, such as oxygen, promote leaf senescence, altogether supporting the idea that phytohormones play a critical role in regulating leaf senescence. Functional analysis of candidate SAGs in LSD revealed that a WRKY transcription factor WRKY75 and a Cys2/His2-type transcription factor AZF2 are positive regulators of leaf senescence and loss-of-function of WRKY75 or AZF2 delayed leaf senescence. We also found that silencing of a protein phosphatase, AtMKP2, promoted early senescence. Collectively, LSD can serve as a comprehensive resource for systematic study of the molecular mechanism of leaf senescence as well as offer candidate genes for functional analyses. | Zhonahai Li Jinying Peng Xing Wen Hongwei Guo | 2012 | Journal of Integrative Plant Biology2012，54，8： | 21 |
| 4 | Application of DVA theory in vibration reduction of carbody with suspended equipment for high-speed EMU显示文摘The theory of dynamic vibration absorber(DVA)was applied to restrain the vibration of carbody for high-speed electric multiple unit(EMU).The carbody was modeled as an Euler-Bernoulli beam with the equipment mounted on the chassis regarded as a DVA.Suspension parameters of the equipment were optimized based on the modal analysis of the beam and parameter optimization of the DVA.Vertical motion equations of the carbody and equipment were derived to study the effect of the suspension parameters on the vibration of carbody,which included the suspension frequency,damping ratio,mounting position and mass.Then a 3D rigid-flexible coupled vehicle system dynamics model was built to simulate the response of carbody and equipment to track excitation.The results show that the equipment mounted on the carbody chassis can be regarded as a DVA to reduce the flexible vibration of carbody,and the optimum suspension frequency can be calculated theoretically with the first-order vertical bending mode of carbody considered.Heavy equipment should be mounted to the carbody center as close as possible to obtain a significant vibration reduction,while light equipment has quite limited contribution to that.Also,a laboratory test was conducted on the full-scale test rig which shows a good agreement with the theoretical analysis and dynamic simulations.The faster the vehicle runs,the more significant are the advantages of the elastic suspension. | SHI HuaiLong LUO Ren WU PingBo ZENG Jing GUO JinYing | 2014 | Science China(Technological Sciences)2014，57，7： | 14 |
| 5 | Effects of Film-Degraded Rice Controlled Release Fertilizer on the Nitrogen Uptake and Yield of the Hybrid Rice显示文摘Field experiment was carried out to nitrogen release characteristics of rice controlled release fertilizer (RCRF) coated with natural and half natural high molecular materials, and to thereof effects on the nitrogen uptake and yield of early and late hybrid rice from 1999 to 2000, with urea as control.In experimental fields regardless of early and late seasons, the amount of N in the plants increased exponentially at the early growth stage (y=abx) and nonlinearly at the middle and late growth stages (y=a+bx+cx2).Rice controlled release fertilizer lengthened the exponential phase and increased the parameter -b/c during the nonlinear phase. Although the N amount of plants was less in rice controlled release fertilizer plots than in urea plots within 20 days after transplanting, at the heading and maturity stages, the N amount of plants in rice controlled release fertilizer plots was above two times greater than in the urea plots due to the higher N recovery, and the high absorption density of N in the nonlinear phase. Tillers pattern and achievement of the maximum tillering stage varied with fertilizer types. The maximum tillering stage in the urea plots occurred a week earlier than in the rice controlled release fertilizer plots. Yield sink size and potential sink size of the plants reflected the N absorption pattern and the amount of N in the plants. The yields of early and late rice with 90 kg N ha-1of rice controlled release fertilizer were respectively increased by 832.7 kg ha-1 and 412.8 kg ha-1 than those with 90 kg N ha-1 of urea, almost equivalent to the yield of early and late hybrid rice with 180 kg N ha-1 of urea. | Zheng Shengxian, Nie Jun, Xiao Jian, Xiong Jinying, and Yi Guoying Hunan Soil and Fertilizer Institute, Changsha 410125, China | 2002 | Hunan Agricultural Science & Technology Newsletter2002，3，2： | 12 |
| 6 | Effect of dry density on ^(125)I diffusion in GMZ bentonite显示文摘Gaomiaozi(GMZ) bentonite is regarded as the favorable candidate backfilling material for a potential repository in China.It is important to understand the diffusion behavior of 125 I in GMZ bentonite and compare the diffusion behavior in GMZ and other types of bentonite like MX-80,Avonlea,etc.Therefore,through-and out-diffusion experiments were conducted to obtain the effective diffusion coefficient(D e) and distribution coefficient(K d).A computer code named Fitting for diffusion coefficient(FDP) was used for the experimental data processing and theoretical modeling.At the dry density of GMZ bentonite from 1600-2000 kg/m 3,the D e values of 125 I were(2.4-20.4) × 10-12 m 2 /s and K d values were constants.At dry density above 1800 kg/m 3,the diffusion behaviors were almost the same,indicating that the anion exclusion was ineffective.Out-diffusion results showed that the species of 125 I may be changed during the diffusion processing.It was probably caused by some organic matters or reducing substances in GMZ bentonite.Since the main composition of bentonite is montmorillonite,similar diffusion parameters were obtained in GMZ and other types of bentonite.The relationship of D e and accessible porosity(acc) could be described by Archie's law with exponent n = 1.2-2.8 for 125 I diffusion in bentonite,whereas n = 2.0 in GMZ bentonite.Furthermore,bentonite with the dry density of 1800 kg/m 3 was proposed as the backfilling materials used in the construction of high level radioactivity waste repository. | WU Tao LI JinYing DAI Wei XIAO GuoPing SHU FuJun YAO Jun SU YuLan SHI Lei | 2012 | Science China Chemistry2012，55，9： | 11 |
| 7 | The rare two-dimensional materials with Dirac cones显示文摘Inspired by the great development of graphene, more and more research has been conducted to seek new two-dimensional(2D) materials with Dirac cones. Although 2D Dirac materials possess many novel properties and physics, they are rare compared with the numerous 2D materials. To provide explanation for the rarity of 2D Dirac materials as well as clues in searching for new Dirac systems, here we review the recent theoretical aspects of various 2D Dirac materials, including graphene, silicene, germanene,graphynes, several boron and carbon sheets, transition-metal oxides(VO2)n/(TiO2)m and(CrO2)n/(TiO2)m, organic and organometallic crystals, so-MoS2, and artiicial latices(electron gases and ultracold atoms). heir structural and electronic properties are summarized. We also investigate how Dirac points emerge, move, and merge in these systems. he von Neumann–Wigner theorem is used to explain the scarcity of Dirac cones in 2D systems, which leads to rigorous requirements on the symmetry,parameters, Fermi level, and band overlap of materials to achieve Dirac cones. Connections between existence of Dirac cones and the structural features are also discussed. | Jinying Wang Shibin Deng Zhongfan Liu Zhirong Liu | 2015 | National Science Review2015，2，1： | 10 |
| 8 | NUMERICAL SIMULATION OF FEEDSTOCK MELT FILLING IN A CYLINDRICAL CAVITY WITH SOLIDIFICATION IN POWDER INJECTION MOLDING显示文摘INTRODUCTIONPowderinjectionmolding(PIM)isaprocesingrouteforfabricatingmetalorceramiccomponentswithcomplexshapeandhighperfor?.. | Qu Xuanhui, Li Yimin, Yue Hongsheng, Mao Jinying, Zhu Lei and Huang Baiyun State Key Laboratory for Powder Metallurgy, Central South University of Technology, Changsha 410083, P. R. China | 1998 | 中国有色金属学会会刊:英文版1998，8，4： | 9 |
| 9 | Influence of equipment excitation on flexible carbody vibration of EMU显示文摘To study the vibration transmission characteristics of a flexible carbody and its suspended equipment, a vertical mathematical model of high-speed electric multiple unit was established with equipment excitation considered.And the dynamic unbalance and impact turbulence excitation from equipment were taken into account in a singlestage and two-stage vibration isolation system, respectively.Results show that the excitation transferred to carbody increases with suspension stiffness but decreases with the equipment mass increasing; the vibration transmission can be reduced by increasing the equipment mass or reduce the suspension stiffness. To avoid vibration resonance, the dynamic unbalance frequency of equipment should be out of the possible range of the carbody flexible modes, and a small stiffness should be applied to reduce the impact turbulence. A small stiffness, however, would result in a large movement of the equipment which is limited by the static deflection requirement, while a great stiffness will transfer high frequency vibration. Therefore, a preferred stiffness should make the suspension frequency of equipment a bit greater than the first bending mode of carbody. Additionally,a 3D rigid-flexible coupled dynamics model was built to verify the mathematical analysis, and they show good agreements. Results show that a two-stage isolation could reduce the excitation transmission and make the vibration of carbody and equipment acceptable. | Huailong Shi Ren Luo Pingbo Wu Jing Zeng Jinying Guo | 2014 | Journal of Modern Transportation2014，22，4： | 8 |
| 10 | Secondary biogenic coalbed gas in some coal fields of China显示文摘The secondary biogenic coalbed gas, a new genetic and energy source type of coalbed gas in China, has been found in Xinji, Liyazhuang and Enhong areas. The essential characteristics of this type of gas are: (i) the major component of the gas is methane, with C1/C1-5 value higher than 0.99, indicating that the gas is part of dry gas; (ii) theδ13C1 value is in the range of -61.7‰to -47.9‰, mostly lower than -55‰, which is much lower than the estimatedδ13C1 value of thermogenic methane according to the thermal evolution degree of the coal rocks (with R0 value from 0.87% to 1.43%), showing the characteristics of the secondary biogenic gas; (iii) theδ5D value of methane ranges from -244‰to -196‰; (iv)δ13C 2 value ranges from -26.7‰to -15.9‰andδ13C 3 value ranges from -10.8‰to -25.3‰, indicating that the heavier hydrocarbons have a thermogenic origin; (v) the content of CO2 is very low, andδ13CCO2 value changes greatly, reflecting a characteristic of secondary change; (vi)δ15N2 value ranges mainly from -1‰to +1‰, indicating N2 derived significantly from air. The negative linear correlation between the contents of N2 and CH4 reflects the activity of bacteria bearing surface water infiltrating into coal beds. The comprehensive tracing indices show that the coalbed gas in the studied areas is the mixed gas of primarily secondary biogenic gas and a part of remnant thermogenic gas. The uplift of coal beds and the development of faults in the studied areas create favorable conditions for the formation of the secondary biogenic gas. | TAO Mingxin WANG Wanchun XIE Guangxin LI Jinying WANG Yanlong ZHANG Xiaojun ZHANG Hong SHI Baoguang GAO Bo | 2005 | Chinese Science Bulletin2005，50，S1： | 8 |
| 11 | The localization of type 2 diabetes susceptibility gene loci in northern Chinese Han families显示文摘We conducted a genome-wide scan, in which 358 well distributed fluorescent dye-labeled microsatellite marker sets were applied in 32 Chinese Han type 2 diabetes families from Northern China to search for the susceptibility gene loci. The data collected from screening all the chromosomes of genome were genotyped by using genescan and genotyping software, then, parametric and non-parametric multipoint test, and affected sib-pair analysis as well, were used to analyze the data. We identified some susceptibility gene loci residing in chromosomes 1,12,18,20, respectively, or precisely, located around D1S214, D1S207, D1S218, D1S235, D12S336, D18S61 and D20S118. The comparison of this result with those from other regions and races reflected the complexity and heterogeneity of type 2 diabetes. | ZHAO Jinying WANG Heng XIONG Momiao HUANG Wei ZUO Jin CHEN Zhu QIANG Boqin SUN Qi LI Yuxiu LIU Qiuying DU Weinan CHEN Jialun DING Wei YUAN Wentao ZHAO Yang XU Hongyan JIN Li FANG Fude | 2000 | Chinese Science Bulletin2000，45，19： | 8 |
| 12 | A novel integrated UV-biofilter system to treat high concentration of gaseous chlorobenzene显示文摘A novel integrated UV-biofilter system using UV reactor as the pretreatment process was setup to trea high concentration of gaseous volatile organic compounds (VOCs). Another control biofilter withou the UV pretreatment was also established to compare the performance of the two systems. Chloro benzene was selected as a model compound. The two systems were operated in parallel under differen inlet chlorobenzene concentrations (500, 1000, 1600, mg·m?3). The experimental results indicate tha the integrated system could eliminate chlorobenzene completely (100% removal efficiency) at the inle concentration of 500 mg·m?3, whereas only 60% removal efficiency was achieved for the control biofi ter. Also the elimination capacity for the organic carbon of the integrated system was much higher tha that of the control biofilter. On the basis of intermediates analysis by Ion Chromatography and Ga Chromatography-Mass Spectrometry, the UV pretreatment has been proven to be able to enhance th performance of the following biofilter by transferring the recalcitrant target to some more biodegrad able and soluble organic products (such as formic acid and chlorophenol). Furthermore, the produce ozone, a harmful by-product from UV photo-degradation, could be easily eliminated by the followin biofiltration process. | WANG Can XI JinYing HU HongYing | 2008 | Chinese Science Bulletin2008，53，17： | 7 |
| 13 | Ultrasonic Nondestructive Signals Processing Based on Matching Pursuit with Gabor Dictionary显示文摘The success of ultrasonic nondestructive testing technology depends not only on the generation and measurement of the desired waveform, but also on the signal processing of the measured waves. The traditional time-domain methods have been partly successful in identifying small cracks, but not so successful in estimating crack size, especially in strong backscattering noise. Sparse signal representation can provide sparse information that represents the signal time-frequency signature, which can also be used in processing ultrasonic nondestructive signals. A novel ultrasonic nondestructive signal processing algorithm based on signal sparse representation is proposed. In order to suppress noise, matching pursuit algorithm with Gabor dictionary is selected as the signal decomposition method. Precise echoes information, such as crack location and size, can be estimated by quantitative analysis with Gabor atom. To verify the performance, the proposed algorithm is applied to computer simulation signal and experimental ultrasonic signals which represent multiple backscattered echoes from a thin metal plate with artificial holes. The results show that this algorithm not only has an excellent performance even when dealing with signals in the presence of strong noise, but also is successful in estimating crack location and size. Moreover, the algorithm can be applied to data compression of ultrasonic nondestructive signal. | GUO Jinku WU Jinying YANG Xiaojun LIU Guangbin | 2011 | Chinese Journal of Mechanical Engineering2011，24，4： | 7 |
| 14 | PIEZOELECTRIC PROPERTIES OF SINGLE-STRAND DNA MOLECULAR BRUSH BIOLAYERS显示文摘纸在没有标签的简历察觉在生物晶片的 nanomechanical 行为上被奉献给调查。薄片由分子的刷子简历层使不能调动由的 Si 层, Ti 层, Au 层和单个海滨的 DNA (ssDNA ) 组成自己组装 thiol 的技术组。不同于以前的观点,例如弯曲力量,弯曲熵并且弯曲电效果,等等,当导致生物晶片弯曲的 nanomechanical 的主要因素,和一种古典宏观的压电的组成的关系被用来描述压电的效果,简历聚合物刷子层的压电的效果被看。有在连续统力学,在统计力学的线性化的 Poisson-Boltzmann 方程和在聚合电解质刷子理论的可伸缩的方法的压电的简历层的一个新把压成薄片的伸臂横梁模型被联合建立在象 nanoscopic 那样的 DNA 薄片和因素的 nanomechanical 偏转之间的一种关系 ssDNA 分子的结构的特征,缓冲区盐集中, DNA 芯片等等的宏观的机械/压电的参数。试验性的数据的曲线试穿证明简历层的压电的常数的符号可以在包装进程期间控制 DNA 芯片的偏转方向。 | Zhang Nenghui Shah Jinying Xing Jingjing | 2007 | Acta Mechanica Solida Sinica2007，20，3： | 6 |
| 15 | Cu Partitioning Behavior and Its Effect on Microstructure and Mechanical Properties of 0.12C-1.33Mn-0.55Cu Q&P Steel显示文摘Cu, as an austenitic stable element, is added to steel in order to suppress the adverse effects of high content of C and Mn on welding. Based on C partitioning, Cu and Mn partitioning can further improve the stability of retained austenite in the intercritical annealing process. A sample of low carbon steel containing Cu was treated by the intercritical annealing, then quenching process(I&Q). Subsequently, another sample was treated by the intercritical annealing, subsequent austenitizing, then quenching and partitioning process(I&Q&P). The effects of element partitioning behavior in intercritical region on the microstructure and mechanical properties of the steel were studied. The results showed that after the I&Q process ferrite and martensite could be obtained, with C, Cu and Mn enriched in the martensite. When intercritically heated at 800 ℃, Cu and Mn were partitioned from ferrite to austenite, which was enhanced gradually as the heating time was increased. This partitioning effect was the most obvious when the sample was heated at 800 ℃ for 40 min. At the early stage of α→γ transformation, the formation of γ was controlled by the partitioning of carbon, while at the later stage, it was mainly affected by the partitioning of Cu and Mn. After the I&Q&P process, the partitioning effect of Cu and Mn element could be retained. C was assembled in retained austenite during the quenching and partitioning process. The strength and elongation of I&Q&P steel was increased by 5 305 MPa% compared with that subjected to Q&P process. The volume fraction of retained autensite was increased from 8.5% to 11.2%. Hence, the content of retained austenite could be improved significantly by Mn and Cu partitioning, which increased the elongation of steel. | 陈连生 HU Baojia XU Jinghui 田亚强 ZHENG Xiaoping SONG Jinying XU Yong | 2017 | Journal of Wuhan University of Technology(Materials Science)2017，32，5： | 6 |
| 16 | Structural insights into Cas13b-guided CRISPR RNA maturation and recognition显示文摘Dear Editor,CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins)systems are RNA-guided adaptive immune systems in prokaryotes.^1,2 Class 2 CRISPR-Cas systems (including type Ⅱ,Ⅴ,and Ⅵ)involve large single effector proteins in complex with crRNA for interference. | Bo Zhang Weiwei Ye Yangmiao Ye Huan Zhou Abdullah F.U.H.Saeed Jing Chen Jinying Lin Vanja Perculija Qi Chen Chun-Jung Chen Ming-Xian Chang Muhammad lqbal Choudhary Songying Ouyang | 2018 | Cell Research2018，28，12： | 5 |
| 17 | MoS2 nanorods with inner caves through synchronous encapsulation of sulfur for high performance Li–S cathodes显示文摘Lithium–sulfur(Li–S)batteries have become one of the most promising candidates for next-generation batteries owing to their high specific capacity,low cost,and environment-friendliness.Many efforts have been made to mitigate the'shuttle effect'through physical adsorption and chemical bonding.MoS2 has been proposed as a cathode material to provide effective anchoring sites for lithium polysulfides(Li PSs),but is still limited by its layer structure.Herein,we designed novel MoS2 nanorods with inner caves based on our previous work,and performed synchronous encapsulation of sulfur during the synthesis process.The outer MoS2 tubular shells physically inhibit the outward diffusion of polysulfide species while the inner particles chemically anchor the polysulfides to prevent shuttling.As the cathode matrix in Li–S batteries,the electrochemical results deliver a high initial discharge capacity of 1213 mAhg^-1 for sulfur at 0.1 C.After cycling at 1 C for 300 cycles,the cells exhibit a capacity decay of only 0.076%per cycle and high average coulombic efficiency over 95%.The tubular MoS2 structure is an innovative and appealing design,which could be regarded as a prospective substrate for the improved performance of Li–S batteries. | Yikun Yi Zechen Liu Pu Yang Tao Wang Xuewen Zhao Hongyang Huang Yonghong Cheng Jinying Zhang Mingtao Li | 2020 | Journal of Energy Chemistry2020，29，6： | 5 |
| 18 | An antioxidant system through conjugating superoxide dismutase onto metal-organic framework for cardiac repair显示文摘Acute myocardial infarction(AMI)remains a dominant origin of morbidity,mortality and disability worldwide.Increases in reactive oxygen species(ROS)are key contributor to excessive cardiac injury after AMI.Here we developed an immobilized enzyme with Superoxide Dismutase(SOD)activity cross-link with Zr-based metal-organic framework(ZrMOF)(SOD-ZrMOF)for mitigate ROS-caused injury.In vitro and in vivo evidence indicates that SOD-ZrMOF exhibits excellent biocompatibility.By efficiently scavenging ROS and suppressing oxidative stress,SOD-ZrMOF can protect the function of mitochondria,reduce cell death and alleviate inflammation.More excitingly,long-term study using an animal model of AMI demonstrated that SOD-ZrMOF can reduce the infarct area,protect cardiac function,promote angiogenesis and inhibit pathological myocardial remodeling.Therefore,SOD-ZrMOF holds great potential as an efficacious and safe nanomaterial treatment for AMI. | Jiacheng Guo Zhenzhen Yang Yongzheng Lu Chunyan Du Chang Cao Bo Wang Xiaoting Yue Zenglei Zhang Yanyan Xu Zhen Qin Tingting Huang Wei Wang Wei Jiang Jinying Zhang Junnan Tang | 2022 | Bioactive Materials2022，7，4： | 4 |
| 19 | HrcQ is necessary for Xanthomonas oryzae pv. oryzae HR-induction in non-host tobacco and pathogenicity in host rice显示文摘Bacterial blight, caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice(Oryza sativa L.) worldwide. The type III secretion system(T3SS) of Xoo, encoded by the hrp(hypersensitive response and pathogenicity) genes, plays critical roles in conferring pathogenicity in host rice and triggering a hypersensitive response(HR) in non-host plants. To investigate the major genes conferring the pathogenicity and avirulence of Xoo, we previously constructed a random Tn5-insertion mutant library of Xoo strain PXO99A. We report here the isolation and characterization of a Tn5-insertion mutant PXM69. Tn5-insertion mutants were screened on indica rice JG30, which is highly susceptible to PXO99A, by leaf-cutting inoculation.Four mutants with reduced virulence were obtained after two rounds of screening. Among them, the mutant PXM69 had completely lost virulence to the rice host and ability to elicit HR in non-host tobacco. Southern blotting analysis showed a single copy of a Tn5-insertion in the genome of PXM69. PCR walking and sequencing analysis revealed that the Tn5 transposon was inserted at nucleotide position 70,192–70,201 in the genome of PXO99A, disrupting the type III hrc(hrp-conserved) gene hrcQ, the first gene in the D operon of the hrp cluster in Xoo. To confirm the relationship between the Tn5-insertion and the avirulence phenotype of PXM69, we used the marker exchange mutagenesis to create a PXO99Amutant, ΔhrcQ::KAN, in which the hrcQ was disrupted by a kanamycin-encoding gene cassette at the same site as that of the Tn5-insertion. ΔhrcQ::KAN showed the same phenotype as mutant PXM69. Reintroduction of the wild-type hrcQ gene partially complemented the pathogenic function of PXM69. RT-PCR and cellulase secretion assays showed that the Tn5-disruption of hrcQ did not affect transcription of downstream genes in the D operon and function of the type II secretion system. Our results provide new insights into the pathogenic functions of clustered hrp genes in Xoo. | Xiaoping Zhang Chunlian Wang Chongke Zheng Jinying Che Yanqiang Li Kaijun Zhao | 2013 | The Crop Journal2013，1，2： | 4 |
| 20 | Liquid biopsies:DNA methylation analyses in circulating cell-free DNA显示文摘Analysis of patient's materials like cells or nucleic acids obtained in a minimally invasive or noninvasive manner through the sampling of blood or other body fluids serves as liquid biopsies, which has huge potential for numerous diagnostic applications. Circulating cell-free DNA(cfDNA) is explored as a prognostic or predictive marker of liquid biopsies with the improvements in genomic and molecular methods. DNA methylation is an important epigenetic marker known to affect gene expression. cfDNA methylation detection is a very promising approach as abnormal distribution of DNA methylation is one of the hallmarks of many cancers and methylation changes occur early during carcinogenesis. This re?view summarizes the various investigational applications of cfDNA methylation and its oxidized de?rivatives as biomarkers for cancer diagnosis, prenatal diagnosis and organ transplantation monitoring.The review also provides a brief overview of the technologies for cfDNA methylation analysis based on next generation sequencing. | Hu Zeng Bo He Chengqi Yi Jinying Peng | 2018 | Journal of Genetics and Genomics2018，45，4： | 4 |
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