|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Antioxidation Activities of Low-Molecular-Weight Gelatin Hydrolysate Isolated from the Sea Cucumber Stichopus japonicus显示文摘Gelatin extracted from the body wall of the sea cucumber (Stichopus japonicus) was hydrolyzed with flavourzyme.Low-molecular-weight gelatin hydrolysate (LMW-GH) of 700-1700 Da was produced using an ultrafiltration membrane bioreactor system.Chemiluminescence analysis revealed that LMW-GH scavenges high free radicals in a concentration-dependent manner;IC50 value for superoxide and hydroxyl radicals was 442 and 285 μg mL-1,respectively.LMW-GH exhibited excellent inhibitory characteristics against melanin synthesis and tyrosinase activity in B16 cells.Furthermore,LMW-GH notably increased intracellular glutathione (GSH),which in turn suppressed melanogenesis.LMW-GH performs antioxidation activity,holding the potential of being used as a valuable ingredient in function foods,cosmetics and pharmaceuticals or nutriceuticals. | WANG Jingfeng WANG Yuming TANG Qingjuan WANG Yi CHANG Yaoguang ZHAO Qin XUE Changhu | 2010 | Journal of Ocean University of China2010，9，1： | 13 |
| 2 | Numerical simulation of the pulsing air separation field based on CFD显示文摘The flow field of pulsing air separation is normally in an unsteady turbulence state.With the application of the basic principles of multiphase turbulent flows,we established the dynamical computational model,which shows a remarkable variation of the unstable pulsing air flow field.CFD(computational fluid dynamics) was used to conduct the numerical simulation of the actual geometric model of the classifier.The inside velocity of the flowing fields was analyzed later.The simulation results indicate that the designed structure of the active pulsing air classifier provided a favorable environment for the separation of the particles with different physical characters by density.We shot the movement behaviors of the typical tracer grains in the active pulsing flow field using a high speed dynamic camera.The displacement and velocity curves of the particles in the continuous impulse periods were then analyzed.The experimental results indicate that the effective separation by density of the particles with the same settling velocity and different ranges of the density and particle size can be achieved in the active pulsing airflow field.The experimental results provide an agreement with the simulation results. | He Jingfeng He Yaqun Zhao Yuemin Duan Chenlong Ye Cuiling | 2012 | International Journal of Mining Science and Technology2012，22，2： | 12 |
| 3 | Numerical simulation and experimental verification of bubble size distribution in an air dense medium fluidized bed显示文摘Bubble size distribution is the basic apparent performance and obvious characteristics in the air dense medium fluidized bed (ADMFB). The approaches of numerical simulation and experimental verification were combined to conduct the further research on the bubble generation and movement behavior. The results show that ADMFB could display favorable expanded characteristics after steady fluidization. With different particle size distributions of magnetite powder as medium solids, we selected an appropriate prediction model for the mean bubble diameter in ADMFB. The comparison results indicate that the mean bubble diameters along the bed heights are 35 mm < D b < 66 mm and 40 mm < D b < 69 mm with the magnetite powder of 0.3 mm+0.15mm and 0.15mm+0.074mm, respectively. The prediction model provides good agreements with the experimental and simulation data. Based on the optimal operating gas velocity distribution, the mixture of magnetite powder and <1mm fine coal as medium solids were utilized to carry out the separation experiment on 6-50mm raw coal. The results show that an optimal separation density d P of 1.73g/cm 3 with a probable error E of 0.07g/cm 3 and a recovery efficiency of 99.97% is achieved, which indicates good separation performance by applying ADMFB. | He Jingfeng Zhao Yuemin Luo Zhenfu He Yaqun Duan Chenlong | 2013 | International Journal of Mining Science and Technology2013，23，3： | 11 |
| 4 | Effect of Sn addition on the mechanical properties and bio-corrosion behavior of cytocompatible Mg-4Zn based alloys显示文摘The mechanical properties and bio-corrosion behaviors of as-extruded Mg-4Zn alloys after Sn addition were investigated,systemati-cally.A small amount of Sn addition to Mg-4Zn alloy slightly improved the mechanical properties for solid solution strengthening,and significantly controlled the bio-corrosion rates.Sn participating in the outer layer film formation as SnO/SnO_(2)resisted the bio-corrosion proceeding.Especially,Mg-4Zn-1.5Sn alloy,with a weight loss rate of 0.45 mm/y and hydrogen evolution rate of 0.099 mL/cm^(2)/day,showed cytotoxicity grade of 0 to MC3T3-E1 cells.The perfect alliance of cytocompatibility,suitable mechanical properties and low bio-corrosion rate demonstrates that this Mg-4Zn-1.5Sn alloy is a promising biodegradable magnesium alloy for orthopedic implants. | Weiyan Jiang Jingfeng Wang Weikang Zhao Qingshan Liu Dianming Jiang Shengfeng Guo | 2019 | Journal of Magnesium and Alloys2019，7，1： | 6 |
| 5 | Processes of coastal ecosystem carbon sequestration and approaches for increasing carbon sink显示文摘The oceans are the largest carbon pools on Earth, and play the role of a 'buffer' in climate change. Blue carbon, the carbon(mainly organic carbon) captured by marine ecosystems, is one of the important mechanisms of marine carbon storage.Blue carbon was initially recognized only in the form of visible coastal plant carbon sequestration. In fact, microorganisms(phytoplankton, bacteria, archaea, viruses, and protozoa), which did not receive much attention in the past, account for more than 90% of the total marine biomass and are the main contributors to blue carbon. Chinese coastal seas, equivalent to 1/3 of China's total land area, have a huge carbon sink potential needing urgently research and development. In this paper, we focus on the processes and mechanisms of coastal ocean's carbon sequestration and the approaches for increasing that sequestration. We discuss the structures of coastal ecosystems, the processes of carbon cycle, and the mechanisms of carbon sequestration. Using the evolution of coastal ocean's carbon sinks in sedimentary records over geologic times, we also discuss the possible effects of natural processes and anthropogenic activities on marine carbon sinks. Finally, we discuss the prospect of using carbon sequestration engineering for increasing coastal ocean's carbon storage capacity. | ZHANG Yao ZHAO MeiXun CUI Qiu FAN Wei QI JiaGuo CHEN Ying ZHANG YongYu GAO KunShan FAN JingFeng WANG GuangYi YAN ChongLing LU HaoLiang LUO YaWei ZHANG ZiLian ZHENG Qiang XIAO Wei JIAO NianZhi | 2017 | Science China Earth Sciences2017，60，5： | 6 |
| 6 | The temporal and spatial fluctuation of the groundwater level along the Tarim Desert Highway显示文摘In this work, a groundwater monitor section along the Tarim Desert Highway was set up, and the groundwater level data were recorded. The temporal and spatial fluctuations of the groundwater level were analyzed based on the groundwater level measurements, hydrologic data, the groundwater/surface water interconversion theory and the groundwater dynamics. The spatial distribution of groundwater is mostly affected by sand dune height and landform variation. The four primary temporal influence factors of groundwater level can be ordered in decreasing significance as: shelterbelt pumping > vertical leakage > river flow change > runoff replenishment. The runoff replenishment has the biggest influence range, and the shelterbelt pumping has the smallest influence range. The groundwater level fluctuates annually in a natural pattern. | FAN JingLong XU XinWen LEI JaQiang ZHAO JingFeng LI ShengYu WANG HaiFeng ZHANG JianGuo ZHOU HongWei | 2008 | Chinese Science Bulletin2008，53，S2： | 6 |
| 7 | A bulk metal/ceramic composite material with a cellular structure显示文摘A bulk metal/ceramic composite material with a honeycomb-like micro-cell structure has been prepared by sintering the spherical Al90Mn9Ce1 alloy powders clad by Al2O3 nano-powder with the spark plasma sintering (SPS) technique. The as-prepared material consists of Al90Mn9Ce1 alloy cell and closed Al2O3 ceramic cell wall. The diameter of the cells is about 20―40 μm, while a thickness of the cell wall is about 1―2 μm. The ultimate compressive strength of the as-sintered materials is about 514 MPa, while its fracture strain is up to about 0.65 %. This composite material might possess good anti-corrosion, thermal endurance and other potential properties due to its unique microstructure. The result shows that the Al90Mn9Ce1/Al2O3 composite powders can be sintered by spark plasma sintering technique despite the large difference in their sintering temperature. This work offers a way of designing and preparing metal/ceramic composite material with functional property. | ZHAO Zhankui YAO Kefu LI Jingfeng | 2006 | Chinese Science Bulletin2006，51，2： | 5 |
| 8 | Strain hardening of as-extruded Mg-xZn(x=1, 2, 3 and 4 wt%) alloys显示文摘The influence of Zn on the strain hardening of as-extruded Mg-x Zn(x = 1, 2, 3 and 4 wt%) magnesium alloys was investigated using uniaxial tensile tests at 10^(-3)s^(-1) at room temperature. The strain hardening rate,the strain hardening exponent and the hardening capacity were obtained from true plastic stress-strain curves. There were almost no second phases in the as-extruded Mg-Zn magnesium alloys. Average grain sizes of the four as-extruded alloys were about 17.8 μm. With increasing Zn content from 1 to 4 wt%, the strain hardening rate increased from 2850 MPa to 6810 MPa at(б-б_(0.2)) = 60 MPa, the strain hardening exponent n increased from 0.160 to 0.203, and the hardening capacity, Hc increased from 1.17 to 2.34.The difference in strain hardening response of these Mg-Zn alloys might be mainly caused by weaker basal texture and more solute atoms in the α-Mg matrix with higher Zn content. | Chaoyue Zhao Xianhua Chen Fusheng Pan Jingfeng Wang Shangyu Gao Teng Tu Chunquan Liu Jiahao Yao Andrej Atrens | 2019 | Journal of Materials Science & Technology2019，35，1： | 4 |
| 9 | Separation performance of fine low-rank coal by vibrated gas-solid fluidized bed for dry coal beneficiation显示文摘 | Jingfeng He Yuemin Zhao Jie Zhao Zhenfu Luo Chenlong Duan Yaqun He | 2015 | Particuology2015，13，6： | 4 |
| 10 | Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model显示文摘Liver transplantation is an established therapy for end-stage liver diseases. Graft rejection occurs unless the recipient receives immunosuppression after transplantation. This study aimed to explore the mechanism of acute rejection of liver allografts in rats pre-treated with total body irradiation to eliminate passenger lymphocytes and to define the role of CD4 + CD25 + regulatory T cells in the induction of immunotolerance in the recipient. Male Lewis rats were used as donors and male DA rats were recipients. Rats were randomly assigned to the following four groups: control group, homogeneity liver transplantation group, idio-immunotolerance group and acute rejection group. After transplantation, the survival time of each group, serum alanine aminotransferase, total bilirubin levels, number of Foxp3 + CD4 + CD25 + regulatory T cells, expression of glucocorticoid-induced tumor necrosis factor receptor on T cell subgroups, histopathology of the hepatic graft and spleen cytotoxic T lymphocyte lytic activity were measured. In the acute rejection group, where donors were preconditioned with total body irradiation before liver transplantation, all recipients died between day 17 and day 21. On day 14, serum alanine aminotransferase increased signifi- cantly to (459.2±76.9) U L -1 , total bilirubin increased to (124.1±33.7) μmol L -1 (P<0.05) and the ratio of Foxp3 + CD4 + CD25 + regulatory T cells decreased significantly to 1.50%±0.50% (P<0.05) compared with the other groups. Analysis of the T cell subpopulations in the acute rejection group varied from the other groups. Histological analysis showed typical changes of acute rejection in the acute rejection group only. Preconditioning of the donors with total body irradiation eliminated passenger lymphocytes of the liver graft, and thus affected the course of tolerance and induced acute rejection after liver transplantation. | ZHANG YeWei ZHAO HeWei BO Lin YANG YinXue LU Xiang SUN JingFeng WEN JianFei HE Xia YIN GuoWen | 2012 | Science China(Life Sciences)2012，55，9： | 4 |
| 11 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 12 | Industrial Structural Change and Economic Growth in China, 1987-2008显示文摘现在的纸从 1987 ~ 2008 在中国经济学习经济生长的来源和结构的变化的性质。用评估工业的贡献到经济生长的方法论,现在的纸证明 post-2000 subperiod 在中国经济作为生长的来源在服务性行业上标记增加的信赖。许多在真实 GDP 的加速或在在与 pre-2000 时期相比的 post-2000 subperiod 的中国的总数劳动生产率生长能被跟踪到增加的贡献从生产服务并且出口生产工业的高科技。证据显示中国经济向国内消费一直在重新平衡并且向生产工业的高科技转移它的出口部门。 | Jingfeng Zhao Jianmin Tang | 2015 | China & World Economy2015，23，2： | 4 |
| 13 | A novel biodegradable Mg-1Zn-0.5Sn alloy:Mechanical properties,corrosion behavior,biocompatibility,and antibacterial activity显示文摘To meet the growing demand for antibacterial implants for bone-implant-associated infection therapy and avoid the adverse effects of secondary surgery,a degradable platform with pH responsiveness and ion-associated antibacterial properties was constructed.A small amount of Sn added to Mg-1Zn alloy reduces the biocorrosion rate,which can be attributed to Sn participation in outer-layer film formation,significantly reducing the biocorrosion rate and hydrogen evolution rate after implantation in vivo.These Mg alloys,which are susceptible to degradation in the acidic bacterial microenvironment,degrade by releasing Mg,Zn and Sn,producing favorably alkaline and antibacterial conditions.Samples with the composition of Mg-1Zn-0.5Sn were found to be beneficial for promoting initial cell adhesion and proliferation,resulting in improved biocompatibility and biosafety.The biocompatibility of this alloy was confirmed by the healthy behavior of animals and the absence of acute or chronic toxicity in the liver,spleen,and kidneys.Our results demonstrate that Mg-1Zn-0.5Sn is safe for biological systems,enabling its efficacious use in biomedical applications. | Weikang Zhao Jingfeng Wang Jiang Weiyang Bo Qiao Yiyang Wang Yuling Li Dianming Jiang | 2020 | Journal of Magnesium and Alloys2020，8，2： | 3 |
| 14 | Development of a novel RNAi therapy:Engineered miR-31 exosomes promoted the healing of diabetic wounds显示文摘Rationale:Chronic wounds associated with diabetes exact a heavy burden on individuals and society and do not have a specific treatment.Exosome therapy is an extension of stem cell therapy,and RNA interference(RNAi)-based therapy is a type of advanced precision therapy.Based on the discovery of chronic wound-related genes in diabetes,we combined exosome therapy and RNAi therapy through an engineering approach for the treatment of diabetic chronic wounds.Methods:We combined exosome therapy and RNAi therapy to establish a precision therapy for diabetes-associated wounds via an engineered exosome approach.Results:First,chronic diabetic wounds express low levels of miR-31-5p compared with nondiabetic wounds,and an miR-31-5p mimic was shown to be effective in promoting the proliferation and migration of three wound-related cell types in vitro.Second,bioinformatics analysis,luciferase reporter assays and western blotting suggested that miR-31-5p promoted angiogenesis,fibrogenesis and reepithelization by inhibiting factor-inhibiting HIF-1(HIF1AN,also named FIH)and epithelial membrane protein-1(EMP-1).Third,engineered miR-31 exosomes were generated as a miR-31-5p RNAi therapeutic agent.In vivo,the engineered miR-31 exosomes promoted diabetic wound healing by enhancing angiogenesis,fibrogenesis and reepithelization.Conclusion:Engineered miR-31 exosomes are an ideal disease pathophysiology-initiated RNAi therapeutic agent for diabetic wounds. | Jinghuan Huang Muyu Yu Wenjing Yin Bo Liang Ang Li Jingfeng Li Xiaolin Li Shichang Zhao Fang Liu | 2021 | Bioactive Materials2021，6，9： | 3 |
| 15 | Improving in vitro and in vivo corrosion resistance and biocompatibility of Mg-1Zn-1Sn alloys by microalloying with Sr显示文摘Magnesium(Mg)and its alloys have attracted attention as potential biodegradable materials in orthopedics due to their mechanical and physical properties,which are compatible with those of human bone.However,the effect of the mismatch between the rapid material degradation and fracture healing caused by the adverse effect of hydrogen(H2),which is generated during degradation,on surrounding bone tissue has severely restricted the application of Mg and its alloys.Thus,the development of new Mg alloys to achieve ideal degradation rates,H2 evolution and mechanical properties is necessary.Herein,a novel Mg-1Zn-1Sn-xSr(x=0,0.2,0.4,and 0.6 wt%)quaternary alloy was developed,and the microstructure,mechanical properties,corrosion behavior and biocompatibility in vitro/vivo were investigated.The results demonstrated that a minor amount of strontium(Sr)(0.2 wt%)enhanced the corrosion resistance and mechanical properties of Mg-1Zn-1Sn alloy through grain refinement and second phase strengthening.Simultaneously,due to the high hydrogen overpotential of tin(Sn),the H2 release of the alloys was significantly reduced.Furthermore,Sr-containing Mg-1Zn-1Sn-based alloys significantly enhanced the viability,adhesion and spreading of MC3T3-E1 cells in vitro due to their unique biological activity and the ability to spontaneously form a network structure layer with micro/nanotopography.A low corrosion rate and improved biocompatibility were also maintained in a rat subcutaneous implantation model.However,excessive Sr(>0.2 wt%)led to a microgalvanic reaction and accelerated corrosion and H2 evolution.Considering the corrosion resistance,H2 evolution,mechanical properties and biocompatibility in vitro and in vivo,Mg-1Zn-1Sn-0.2Sr alloy has tremendous potential for clinical applications. | Yafeng Wen Qingshan Liu Jingfeng Wang Qiming Yang Weikang Zhao Bo Qiao Yuling Li Dianming Jiang | 2021 | Bioactive Materials2021，6，12： | 3 |
| 16 | Influence of Ni interlayer on interfacial microstructure and mechanical properties of Ti-6Al-4V/AZ91D bimetals fabricated by a solid–liquid compound casting process显示文摘The solid–liquid compound casting of Mg-AZ91D and Ti-TC4 alloys was developed by using pure Ni electro-deposited coating.The pouring temperatures of 660℃,690℃,720℃and 750℃were chosen to investigated the effects of casting temperatures on microstructural evolution,properties,and fracture behaviors of Ni-coated TC4/AZ91D bimetals by the solid–liquid compound casting(SLCC).The scanning electron microscopy(SEM)and the energy dispersive spectroscopy(EDS)results showed that the interfacial zone mainly composed of nickel,Mg_(2)Ni and Mg-Al-Ni in the bimetals cast at 660℃.As the pouring temperature was increased to 750℃,the width of the interface zone,which mainly composed ofδ(Mg),Mg_(2)Ni,Mg-Al-Ni,Mg_(3)TiNi_(2) and Al_(3)Ni,gradually increased.The microhardness tests showed that the micro-hardness of the interface zone was smaller than that of TC4 substrate but larger than that of the cast AZ91D matrix.At the pouring temperature of 720℃,the Ni-coated TC4/AZ91D bimetals had the most typical homogeneous interface,which had granular Mg-Al-Ni ternary phase but no ribbon-like Al3Ni binary phase,and achieved the highest shear strength of 97.35MPa.Meanwhile,further fracture behavior analysis showed that most fracture failure of Ni-coated TC4/AZ91D bimetals occurred at the Mg_(2)Ni+δ(Mg)eutectic structure and Al_(3)Ni hard intermetallic. | Fulin Wen Jianhua Zhao Miaowang Yuan Jingfeng Wang Dengzhi Zheng Jinyong Zhang Ke He Jingjing Shangguan Yu Guo | 2021 | Journal of Magnesium and Alloys2021，9，4： | 3 |
| 17 | Activation of transmembrane receptor tyrosine kinase DDR1-STAT3 cascade by extracellular matrix remodeling promotes liver metastatic colonization in uveal melanoma显示文摘Colonization is believed a rate-limiting step of metastasis cascade.However,its underlying mechanism is not well understood.Uveal melanoma(UM),which is featured with single organ liver metastasis,may provide a simplified model for realizing the complicated colonization process.Because DDR1 was identified to be overexpressed in UM cell lines and specimens,and abundant pathological deposition of extracellular matrix collagen,a type of DDR1 ligand,was noted in the microenvironment of liver in metastatic patients with UM,we postulated the hypothesis that DDR1 and its ligand might ignite the interaction between UM cells and their surrounding niche of liver thereby conferring strengthened survival,proliferation,sternness and eventually promoting metastatic colonization in liver.We tested this hypothesis and found that DDR1 promoted these malignant cellular phenotypes and facilitated metastatic colonization of UM in liver.Mechanistically,UM cells secreted TGF-β1 which induced quiescent hepatic stellate cells(qHSCs)into activated HSCs(aHSCs)which secreted collagen type I.Such a remodeling of extracellular matrix,in turn,activated DDR1,strengthening survival through upregulating STAT3-dependent Mcl-1 expression,enhancing sternness via upregulating STAT3-dependent S0X2,and promoting clonogenicity in cancer cells.Targeting DDR1 by using 7rh,a specific inhibitor,repressed proliferation and survival in vitro and in vivo outgrowth.More importantly,targeting cancer cells by pharmacological inactivation of DDR1 or targeting microenvironmental TGF-β1-collagen I loop exhibited a prominent anti-metastasis effect in mice.In conclusion,targeting DDR1 signaling and TGF-β1 signaling may be a novel approach to diminish hepatic metastasis in UM. | Wei Dai Shenglan Liu Shubo Wang Li Zhao Xiao Yang Jingfeng Zhou Yun Wang Jing Zhang Ping Zhang Ke Ding Yangqiu Li Jingxuan Pan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
| 18 | Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea显示文摘This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants. | Quanrui Chen Jingfeng Fan Jie Su Hongxia Ming Zhihao Sun Mengfei Li Xiaohui Zhao Yantao Wang Yingxue Zhang Huizhen Zhang Yuan Jin Xiaowan Ma Bin Wang | 2020 | Acta Oceanologica Sinica2020，39，2： | 2 |
| 19 | Significance of ST-segment elevation in lead aVR显示文摘To the editor:The aVR lead has been frequently overlooked in the past.Our study indicates that hypertrophic cardiomyopathy needs to be considered in the differential diagnosis of ST-segment elevation in aVR.A 21-year-old woman presented with breathlessness.Over a period of 14 months,she had been unable to play sports owing to dyspnea on exertion.She denied any chest pain,palpitation.She had no prior illness or cardiovascular risk factors.Her physical examination was unremarkable.The electrocardiogram obtained at the time of presentation (Figure 1) showed distinct ST-segment depressions in eight of the 12 electrocardiogram leads.The most striking electrocardiogram abnormality was characterized by a prominent ST-segment elevation of almost 2 mm (0.2 mV) in the aVR lead. | Zhao Yong Wang Jingfeng Huang Guibao Ding Chunhua | 2014 | Chinese Medical Journal2014，，16： | 2 |
| 20 | Effects of Epstein-Barr virus infection on the development of multiple myeloma after liver transplantation显示文摘Reduced cellular immune function in patients after liver transplantation easily results in many types of viral infections,such as Epstein-Barr virus.Epstein-Barr virus is a γ-herpesvirus and is related to many malignant diseases,especially epithelial and lymph tumors.The abnormal interaction of cluster of differentiation 40 with cluster of differentiation 40 ligand and expression of cluster of differentiation 40 ligand are considered closely related to the development of myeloma cells.This study explored the influence and mechanism of Epstein-Barr virus infection on the phenotype and biological behavior of myeloma cells after liver transplantation.Flow cytometry was used to detect coexpression of cluster of differentiation 40 and cluster of differentiation 40 ligand in 10 samples of freshly isolated multiple myeloma cells.Cluster of differentiation 40 and cluster of differentiation 40 ligand were coexpressed in sample Nos.5,8,9,and 10,particularly in sample No.5.Western blot analysis was used to detect the expression of the Epstein-Barr virus antigens latent membrane protein 1 and Epstein-Barr virus nuclear antigen 2 in the multiple myeloma cell line RPMI 8226 infected with Epstein-Barr virus.The antigen expression indicated that Epstein-Barr virus can infect multiple myeloma virus cells in vitro.Reverse transcription-polymerase chain reaction revealed upregulated expression of cluster of differentiation 40 ligand on the infected RPMI 8226 cells,which may be involved in the anti-apoptosis activity of the infected cells.Confocal microscopy showed that pairs of molecules of cluster of differentiation 40,cluster of differentiation 40 ligand,and latent membrane protein 1 were colocalized on the surface of the infected cells.CXC chemokine receptor 4 was upregulated on the RPMI 8226 cells after Epstein-Barr virus infection.The migratory ability of the infected cells improved in the presence of the chemokine stromal cell-derived factor-1α.Anti-apoptosis and migration are known important biological characteristics of malignant cells.Our results indicate the involvement of Epstein-Barr virus in the origin and development of multiple myeloma.The risk of multiple myeloma increases when Epstein-Barr virus infects B cells in the germinal center,which may result in an anti-apoptosis effect of B cells and an improved ability to migrate from the germinal center to peripheral blood. | ZHANG YeWei ZHAO HeWei HE Xia ZHENG SuWen WANG TaiHong YAN DongLiang SUN JingFeng LU Xiang WEN JianFei LAU Wan Yee | 2012 | Science China(Life Sciences)2012，55，8： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员