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您的检索式：作者名="Jane Morrell"
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| 1 | Suitability and effectiveness of single layer centrifugation using Androcoll-P in the cryopreservation protocol for boar spermatozoa显示文摘 | Maria J. Martinez-Alborcia Jane M. Morrell Maria A. Gil Isabel Barranco Carolina Maside Diego V. Alkmin Inmaculada Parrilla Emilio A. Martinez Jordi Roca | 2013 | Animal Reproduction Science2013，，： | 1 |
| 2 | New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models显示文摘Background: Sperm DNA fragmentation(sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model(linear regression, exponential or polynomial) for DNA fragmentation over time in frozen-thawed donkey semen.Results: After submitting post-thaw semen samples to no centrifugation(UDC), sperm washing(SW) or single layer centrifugation(SLC) protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or UDC.Coefficient of determination(R^2) values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation(aSDF) were obtained for SW, fol owed by SLC and UDC.Conclusion: SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule. | Isabel Ortiz Jesus Dorado Jane Morrell Jaime Gosalvez Francisco Crespo Juan M.Jimenez Manuel Hidalgo | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 0 |
| 3 | Animal Reproduction Technologies-FuturePerspectives显示文摘Reproductive biotechnologies, such as artificial insemination, sperm selection and embryo technologies, offer possibilitiesfor animal producers to increase reproductive efficiency. There have been many significant developments in reproductivebiotechnologies over the last few decades, e.g., in sperm handling and preservation, in vitro embryo production and preservation,sexing and cloning. This review discusses some of the key changes that have occurred and explores their potential for increasing thereproductive efficiency of domestic animals in the future. As a consequence, they also offer opportunities to facilitate or accelerategenetic selection. If properly used, they may contribute to increase the sustainability of animal production. The role of epigenetics ininfluencing phenotype is also considered. | Jane Morrell Patrice Humblot | 2016 | Journal of Agricultural Science and Technology(A)2016，6，5： | 0 |
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