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| 1 | R-spondin-2 is a Wnt agonist that regulates osteoblast activity and bone mass显示文摘The R-spondin family of proteins are Wnt agonists, and the complete embryonic disruption of Rspo2 results in skeletal developmental defects that recapitulate the phenotype observed with Lrp5/6 deficiency. Previous work has shown that R-spondin-2(Rspo2, RSPO2) is both highly expressed in Wnt-stimulated pre-osteoblasts and its overexpression induces osteoblast differentiation in the same cells, supporting its putative role as a positive autocrine regulator of osteoblastogenesis. However, the role of Rspo2 in regulating osteoblastogenesis and bone formation in postnatal bone has not been explored. Here we show that limb-bud progenitor cells from Rspo2 knockout mice undergo reduced mineralization during osteoblastogenesis in vitro and have a corresponding alteration in their osteogenic gene expression profile. We also generated the first Rspo2 conditional knockout(Rspo2 floxed) mouse and disrupted Rspo2 expression in osteoblast-lineage cells by crossing to the Osteocalcin-Cre mouse line(OcnCre + Rspo2 ^(f/f)). Ocn-Cre + Rspo2 f/fmale and female mice at 1, 3, and 6 months were examined. Ocn-Cre + Rspo2 f/fmice are decreased in overall body size compared to their control littermates and have decreased bone mass. Histomorphometric analysis of1-month-old mice revealed a similar number of osteoblasts and mineralizing surface per bone surface with a simultaneous decrease in mineral apposition and bone formation rates. Consistent with this observation, serum osteocalcin in 3-month-old Ocn-Cre +Rspo2 f/fwas reduced, and bone marrow-mesenchymal stem cells from Ocn-Cre + Rspo2 f/fmice undergo less mineralization in vitro.Finally, gene expression analysis and immunohistochemistry of mature bone shows reduced beta-catenin signaling in Ocn-Cre +Rspo2 f/f. Overall, RSPO2 reduces osteoblastogenesis and mineralization, leading to reduced bone mass. | M.Noelle Knight Kannan Karuppaiah Michele Lowe Sarthak Mohanty Robert L.Zondervan Sheila Bell Jaimo Ahn Kurt D.Hankenson | 2018 | Bone Research2018，6，3： | 5 |
| 2 | Two-dimensional and three-dimensional computed tomography for the classification and characterisation of tibial plateau fractures显示文摘 | Job N. Doornberg Maarten V. Rademakers Michel P. van den Bekerom Gino M. Kerkhoffs Jaimo Ahn E.Ph. Steller Peter Kloen | 2011 | Injury2011，，12： | 1 |
| 3 | Systematic review of cemented and uncemented hemiarthroplasty outcomes for femoral neck fractures显示文摘 | Jaimo Ahn Man LX Park SD | 2008 | Clin Orthop Relat Res2008，466，10： | 1 |
| 4 | Notch Signaling Promotes Osteoclast Maturation and Resorptive Activity显示文摘 | Jason W. Ashley Jaimo Ahn Kurt D. Hankenson | 2015 | J. Cell. Biochem2015，，11： | 1 |
| 5 | Dual Plating for Fractures of the Distal Third of the Humeral Shaft显示文摘 | Mark L Prasarn Jaimo Ahn Omesh Paul Elizabeth M Morris Stephen P Kalandiak David L Helfet Dean G Lorich | 2011 | Journal of Orthopaedic Trauma2011，，1： | 1 |
| 6 | Systematic Review of Cemented and Uncemented Hemiarthroplasty Outcomes for Femoral Neck Fractures显示文摘 | Jaimo Ahn MD PhD Li-Xing Man MD MSc SangDo Park MD Jeffrey F. Sodl MD John L. Esterhai MD | 2008 | Clinical Orthopaedics and Related Research2008，，10： | 1 |
| 7 | Modulation of bone morphogenetic protein antagonists to stimulate clinical osteogenesis显示文摘 | Jaimo Ahn David J.J. de Gorter Mark Prasarn David L. Helfet Peter Kloen | 2009 | Bioscience Hypotheses2009，，5： | 1 |
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