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| 1 | The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance显示文摘真核细胞的有机体有允许错误褶层或 unassembled 蛋白质在 endoplasmic 蜂窝胃被保留的质量控制的机制(嗯) 并且随后由嗯联系降级(ERAD ) 降级了。ERAD 小径很好在酵母和哺乳动物被学习;然而,植物 ERAD 的生物功能没被报导。通过分子、细胞的生物途径,我们发现 ERAD 是必要的让植物克服盐应力。在蛋白质在植物房间增加了的盐处理 ubiquitinated 之上,特别展开了快速在 ER 积累了并且随后导致了的蛋白质嗯压力回答。在 ERAD 小径的 HRD1/HRD3 建筑群的 HRD3A 的缺点导致了展开的蛋白质反应(UPR ) 的改变,腌的增加的植物敏感,和在植物房间的 ERAD 底层的保留。而且,我们证明从 ER 的那个 Ca 2+ 版本涉及 UPR 的举起,反应的氧种类(ROS ) 参与 ERAD 相关的植物盐反应小径。 | Lijing Liu Feng Cui Qingliang Li Bojiao Yin Huawei Zhang Baoying Lin Zaorong Wu Ran Xia Sanyuan Tang Qi Xie | 2011 | Cell Research2011，21，6： | 19 |
| 2 | Manipulating mRNA splicing by base editing in plants显示文摘Precursor-mRNAs(pre-mRNA) can be processed into one or more mature m RNA isoforms through constitutive or alternative splicing pathways. Constitutive splicing of pre-mRNA plays critical roles in gene expressional regulation, such as intronmediated enhancement(IME), whereas alternative splicing(AS) dramatically increases the protein diversity and gene functional regulation. However, the unavailability of mutants for individual spliced isoforms in plants has been a major limitation in studying the function of mRNA splicing. Here, we describe an efficient tool for manipulating the splicing of plant genes. Using a Cas9-directed base editor, we converted the 5′ splice sites in four Arabidopsis genes from the activated GT form to the inactive AT form. Silencing the AS of HAB 1.1(encoding a type 2 C phosphatase) validated its function in abscisic acid signaling, while perturbing the AS of RS31 A revealed its functional involvement in plant response to genotoxic treatment for the first time. Lastly,altering the constitutive splicing of Act2 via base editing facilitated the analysis of IME. This strategy provides an efficient tool for investigating the function and regulation of gene splicing in plants and other eukaryotes. | Chenxiao Xue Huawei Zhang Qiupeng Lin Rong Fan Caixia Gao | 2018 | Science China(Life Sciences)2018，61，11： | 18 |
| 3 | Application of magnetic particles modified with amino groups to adsorb copper ions in aqueous solution显示文摘A magnetic adsorbent can be easily recovered from treated water by magnetic force,without requiring further downstream treatment.In this research,amine-functionalized silica magnetite has been synthesized using N-[3-(trimethoxysilyl)propyl]-ethylenediamine(TPED) as a surface modification agent.The synthesized magnetic amine adsorbents were used to adsorb copper ions in an aqueous solution in a batch system,and the maximum adsorption was found to occur at pH 5.5 ± 0.1.The adsorption equilibrium data fitted the Langmuir isotherm equation reasonably well,with a maximum adsorption capacity of 10.41 mg/g.A pseudo second-order model could best describe the adsorption kinetics,and the derived activation energy was 26.92 kJ/mol.The optimum condition to desorb Cu2+ from NH2/SiO2/Fe3O4 was provided by a solution with 0.1 mol/L HNO3. | Yafen Lin Huawei Chen Kaelong Lin Boryann Chen Chyowsan Chiou | 2011 | Journal of Environmental Sciences2011，23，1： | 4 |
| 4 | Identification of the varietal origin of processed loose-leaf tea based on analysis of a single leaf by SNP nanofluidic array显示文摘Tea is an important cash crop, representing a $40 billion-a-year global market. Differentiation of the tea market has resulted in increasing demand for tea products that are sustainably and responsibly produced. Tea authentication is important because of growing concerns about fraud involving premium tea products. Analytical technologies are needed for protection and value enhancement of high-quality brands. For loose-leaf teas, the challenge is that the authentication needs to be established on the basis of a single leaf, so that the products can be traced back to the original varieties. A new generation of molecular markers offers an ideal solution for authentication of processed agricultural products. Using a nanofluidic array to identify variant SNP sequences, we tested genetic identities using DNA extracted from single leaves of 14 processed commercial tea products. Based on the profiles of 60 SNP markers, the genetic identity of each tea sample was unambiguously identified by multilocus matching and ordination analysis. Results for repeated samples of multiple tea leaves from the same products(using three independent DNA extractions) showed 100% concordance, showing that the nanofluidic system is a reliable platform for generating tea DNA fingerprints with high accuracy. The method worked well on green, oolong, and black teas, and can handle a large number of samples in a short period of time. It is robust and cost-effective, thus showing high potential for practical application in the value chain of the tea industry. | Wanping Fang Lyndel W.Meinhardt Huawei Tan Lin Zhou Sue Mischke Xinghua Wang Dapeng Zhang | 2016 | The Crop Journal2016，4，4： | 4 |
| 5 | Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice显示文摘In study,we aimed to determine the mechanisms underlying the gastroprotective effects of sodium copper chlorophyllin(SCC)against ethanol-induced gastric ulcer injury in mice.First,the gastroprotective effects of SCC against gastric ulcer induced by ethanol were assessed.Then,biochemical,histopathological,immunohistochemistry assays,and western blot analysis were conducted to determine the possible mechanisms of action underlying the effects of SCC.Compared to the effects of omeprazole(OME)in a confirmed mouse model of ethanol-induced gastric ulcer injury,treatment with various doses of SCC resulted in up-regulation of Bcl-2 and down-regulation of the pro-apoptotic protein Bax.Significant decreases in the levels of the malondialdehyde(MDA),myeloperoxidase(MPO),and NO in the gastric tissues were observed.Furthermore,inflammatory cytokine analysis revealed that SCC treatment inhibited the expressions of TNF-αand IL-6,greatly reduced the phosphorylation level of IκB,and repressed the nuclear translocation of NF-κB p65,which demonstrated that SCC inhibited the activation of the NF-κB pathway.The present findings suggest that the protective effects of SCC may be beneficial as a potential preventive and therapeutic agent for gastric ulcer through the NF-κB pathway.Taken together,SCC administration significantly decreased the levels of MPO,NO,and MDA in gastric tissue and exerted a powerful anti-inflammatory activity as demonstrated by reduction in the secretions of proinflammatory mediators such as IL-6 and TNF-α in the serum of mice exposed to ethanol. | Huawei Lv Yan Lin Peigang Liu Weiqing Liang Kemin Wei Jinbao Pu Hongjian Zhang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 2 |
| 6 | Changes in Protein Content and Chitinase and β-1,3- glucanase Activities of Rice with Blast Resistance Induced by Ag-antibiotic 702显示文摘[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitinase and β-1,3-glucanase activities in rice. [Method]At the fourth leaf stage of Luliangyou 996,15 μg/m L Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. All treatments were inoculated with spore fluid of Magnaporthe grisea at 48 h post spraying,and the rice inoculated with only distilled water was used as blank control. The enzymes activities( endochitinase,exochitinase and β-1,3-glucanase) and total protein content in rice leaves were determined every 24 h within 168 h post spraying. [Result] Compared with the blank control,the rice inoculated with spore fluid of M. grisea could significantly increase the total protein content and the activities of β-1,3-glucanase and chitinase. The induction effect of Ag-antibiotics 702 exceeded that of Validamycin treatment. And the changes in activities of β-1,3-glucanase and chitinase had obvious synchronicity. [Conclusion] Ag-antibiotic 702 can significantly improve the total protein content and the activities of β-1,3-glucanase and chitinase,thus enhancing the resistance to rice blast. | Hu Neng Tu Xiaorong Li Kuntai Ding Hao Li Huan Zhang Huawei Tu Guoquan Huang Lin | 2017 | Plant Diseases and Pests2017，8，4： | 2 |
| 7 | The Impact of Green Finance on the Ecologicalization of Urban Industrial Structure-Based on GMM of Dynamic Panel System显示文摘Although a number of studies have been published in the general area on various factors affecting the ecologicalization of urban industrial structure,little work has been carried out for empirical studies quantitatively analyzing the relevance between green finance development and the ecologicalization of urban industrial structure.Therefore,based on a comprehensive index of green finance development,this research employs panel data of target cities1 for the period 2012–2020 to explore the influence of green finance on the ecologicalization of urban industrial structure.The empirical results show that green finance development significantly improves the ecologicalization level of urban industrial structure.In addition,it is found that green finance plays a stronger role in promoting the ecologicalization of industrial structure in economically developed regions than in economically underdeveloped regions2.The research results can provide a valuable policy reference for urban green financial market planning and green product innovation. | Kai Lin Huawei Zhao | 2022 | Journal of Artificial Intelligence and Technology2022，2，3： | 1 |
| 8 | Neurogranin as an important regulator in swimming training to improve the spatial memory dysfunction of mice with chronic cerebral hypoperfusion显示文摘Background:Vascular cognitive impairment caused by chronic cerebral hypoperfusion(CCH)has become a hot issue worldwide.Aerobic exercise positively contributes to the preservation or restoration of cognitive abilities;however,the specific mechanism has remained inconclusive.And recent studies found that neurogranin(Ng)is a potential biomarker for cognitive impairment.This study aims to investigate the underlying role of Ng in swimming training to improve cognitive impairment.Methods:To test this hypothesis,the clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(Cas9)system was utilized to construct a strain of Ng conditional knockout(Ng cKO)mice,and bilateral common carotid artery stenosis(BCAS)surgery was performed to prepare the model.In Experiment 1,2-month-old male and female transgenic mice were divided into a control group(wild-type littermate,n=9)and a Ng cKO group(n=9).Then,2-month-old male and female C57BL/6 mice were divided into a sham group(C57BL/6,n=12)and a BCAS group(n=12).In Experiment 2,2-month-old male and female mice were divided into a sham group(wild-type littermate,n=12),BCAS group(n=12),swim group(n=12),BCAS+Ng cKO group(n=12),and swim+Ng cKO group(n=12).Then,7 days after BCAS,mice were given swimming training for 5 weeks(1 week for adaptation and 4 weeks for training,5 days a week,60 min a day).After intervention,laser speckle was used to detect cerebral blood perfusion in the mice,and the T maze and Morris water maze were adopted to test their spatial memory.Furthermore,electrophysiology and Western blotting were conducted to record long-term potential and observe the expressions of Ca^(2+)pathway-related proteins,respectively.Immunohistochemistry was applied to analyze the expression of relevant markers in neuronal damage,inflammation,and white matter injury.Results:The figures showed that spatial memory impairment was detected in Ng cKO mice,and a sharp decline of cerebral blood flow and an impairment of progressive spatial memory were observed in BCAS mice.Regular swimming training improved the spatial memory impairment of BCAS mice.This was achieved by preventing long-term potential damage and reversing the decline of Ca^(2+)signal transduction pathway-related proteins.At the same time,the results suggested that swimming also led to improvements in neuronal death,inflammation,and white matter injury induced by CCH.Further study adopted the use of Ng cKO transgenic mice,and the results indicated that the positive effects of swimming training on cognitive impairments,synaptic plasticity,and related pathological changes caused by CCH could be abolished by the knockout of Ng.Conclusion:Swimming training can mediate the expression of Ng to enhance hippocampal synaptic plasticity and improve related pathological changes induced by CCH,thereby ameliorating the spatial memory impairment of vascular cognitive impairment. | Huawei Lin Jiayong Zhang Yaling Dai Huanhuan Liu Xiaojun He Lewen Chen Jing Tao Chaohui Li Weilin Liu | 2023 | Journal of Sport and Health Science2023，12，1： | 1 |
| 9 | Mean shift- based clustering analysis of multispectral remote sensing imagery 显示文摘 | Shukui Bo Lin Ding Huawei Li | 2009 | International Journal of Remote Sensing2009，30，4： | 1 |
| 10 | The First Reported Case of COVID-19 and Plasmodium ovale Malaria Coinfection—Guangdong Province,China,January 2021显示文摘The current coronavirus disease 2019(COVID-19)pandemic has posed an unprecedented challenge to global health(1–3).The clinical symptoms of COVID-19 are similar to those of malaria,such as fever,myalgia,fatigue,headache,and gastrointestinal symptoms.Due to the heavy burden of medical services and the possible shortage of resources caused by the long-term COVID-19 pandemic,the risk of coinfection of malaria and COVID-19 is a matter of particular concern.Here we reported the first case of COVID-19 coinfection with Plasmodium ovale(P.ovale)malaria in a 47-year-old man who had been previously diagnosed with Plasmodium infection and incompletely treated with antimalarial drugs. | Muxin Chen Shitong Gao Lin Ai Jiaxu Chen Tiejian Feng Zhigao Chen Xiaomin Zhang Shaoyu Deng Zidan Lin Yijun Tang Qian Zhang Gengcheng He Huawei Xiong Xiao-nong Zhou Renli Zhang Dana Huang | 2021 | China CDC weekly2021，3，21： | 0 |
| 11 | Classification of aviation incident causes using LGBM with improved cross-validation显示文摘Aviation accidents are currently one of the leading causes of significant injuries and deaths worldwide. This entices researchers to investigate aircraft safety using data analysis approaches based on an advanced machine learning algorithm.To assess aviation safety and identify the causes of incidents, a classification model with light gradient boosting machine (LGBM)based on the aviation safety reporting system (ASRS) has been developed. It is improved by k-fold cross-validation with hybrid sampling model (HSCV), which may boost classification performance and maintain data balance. The results show that employing the LGBM-HSCV model can significantly improve accuracy while alleviating data imbalance. Vertical comparison with other cross-validation (CV) methods and lateral comparison with different fold times comprise the comparative approach. Aside from the comparison, two further CV approaches based on the improved method in this study are discussed:one with a different sampling and folding order, and the other with more CV. According to the assessment indices with different methods, the LGBMHSCV model proposed here is effective at detecting incident causes. The improved model for imbalanced data categorization proposed may serve as a point of reference for similar data processing, and the model’s accurate identification of civil aviation incident causes can assist to improve civil aviation safety. | NI Xiaomei WANG Huawei CHEN Lingzi LIN Ruiguan | 2024 | Journal of Systems Engineering and Electronics2024，35，2： | 0 |
| 12 | Reconfigurable Vortex-like Paramagnetic Nanoparticle Swarm with Upstream Motility and High Body-length Ratio Velocity显示文摘Drug delivery systems with high-targeted doses can minimize excipients,reduce side effects,and improve efficacy.Human blood circulation is a complex circulatory system,and the motion control of microrobots in the static flow field in vitro is completely different from in vivo.How to achieve precise counterflow motion for targeted drug delivery without vascular blockage and immune rejection is the biggest challenge for micro-nano robots.Here,we propose a control method that enables vortex-like paramagnetic nanoparticle swarm(VPNS)to move upstream against the flow.By mimicking the clustering motion of wild herring schools and the rolling of leukocytes,VPNS are incredibly stable even when subjected to high-intensity jet impacts in the blood environment,can travel upstream,anchor at the target location,and dissipate when the magnetic field is withdrawn,which greatly reduces the risk of thrombosis.VPNS can also upstream along the vessel wall without an additional energy source and has a marked targeted therapeutic effect on subcutaneous tumors. | Luyao Wang Han Gao Hongyan Sun Yiming Ji Li Song Lina Jia Chutian Wang Chan Li Deyuan Zhang Ye Xu Huawei Chen Lin Feng | 2023 | Research2023，，4： | 0 |
| 13 | Single-frequency upconverted laser generation by phase summation显示文摘The phase summation effect in sum-frequency mixing process is utilized to avoid a nonlinearity obstacle in the power scaling of single-frequency visible or ultraviolet lasers.Two single-frequency fundamental lasers are spectrally broadened by phase modulation to suppress stimulated Brillouin scattering in fiber amplifier and achieve higher power.After sum-frequency mixing in a nonlinear optical crystal,the upconverted laser returns to single frequency due to phase summation,when the phase modulations on two fundamental lasers have a similar amplitude but opposite sign.The method was experimentally proved in a Raman fiber amplifier-based laser system,which generated a power-scalable sideband-free single-frequency 590 nm laser.The proposal manifests the importance of phase operation in wave-mixing processes for precision laser technology. | Xin Zeng Shuzhen Cui Huawei Jiang Bowen Ruan Xin Cheng Jiaqi Zhou Zhiquan Lin Xuezong Yang Weibiao Chen Yan Feng | 2023 | High Power Laser Science and Engineering2023，11，2： | 0 |
| 14 | High-power 1560 nm single-frequency erbium fiber amplifier core-pumped at 1480 nm-ERRATUM显示文摘The publisher apologises that due to a publishing error this article was published into volume 10(2022)rather than volume 11.Unfortunately,we are unable to correct this error and so this article has now been updated to confirm the publishing volume as volume 10(2022).Any accepted and publication dates associated with the article are still correct. | Xin Cheng Zhiquan Lin Xuezong Yang Shuizhen Cui Xin Zeng Huawei Jiang Yan Feng | 2023 | High Power Laser Science and Engineering2023，11，2： | 0 |
| 15 | Effect of Mn Doping ZnO on Morphology and Magnetism Synthesized by Hydrothermal Method显示文摘The diluted magnetic semiconductor Zn1-xMnxO crystals were synthesized at 430 ℃ for 24 h by hydrothermal method. The mineralizer is 3 mol·L-1 KOH, and fill factor is 35%. The Mn concentration in crystal was determined by electron probe. The atomic percentage of Mn in crystal increases as the concentration of MnCl2·6H2O in precursor increases. The maximum of the Mn concentration in crystal is above 8%. The morphology of the crystal varies with the concentration of MnCl2·6H2O in precursor increases. And they all show the hexagonal feature of ZnO wurtzite. The measurement of magnetism was carried out by using a superconducting quantum interference device (SQUID). The crystals show low-temperature ferromagnetism with Curie temperature of 50 K. | Wei Zhiren Liu Chao Li Jun Ge Shiyan Lin Lin Zhang Huawei Dong Guoyi | 2006 | Journal of Rare Earths2006，24，z1： | 0 |
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