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| 1 | Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars显示文摘D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in nature.Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase.In this study,the crystal structure of the C.cellulolyticum DPEase was determined.The enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active site.Additional crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also determined.From the complex structures of C.cellulolyticum DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues.Accordingly,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here.These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun | 2012 | Protein & Cell2012，3，2： | 6 |
| 2 | Design and Analysis of Biomimetic Nose Cone for Morphing of Aerospace Vehicle显示文摘当穿透并且重入空气时, Morphing 能力绝对是重要的让太空车辆获得气体动力学,活动性和飞行控制的占优势的功能。然而,为存在太空车辆的挑战尚待活泼地改变它鼻子锥的结构。这份报纸在由调整它的生物形态的形状提高蜜蜂的飞行特征的蜜蜂腹部上执行很多观察实验。morphing 结构从蜜蜂腹部被采用改进轴的可伸缩性和太空车辆的弯曲性质,它能导致超级演习的飞行性能。与最佳设计和拓扑学的方法结合了,新仿生的 morphing 结构被建议并且适用于太空车辆的 morphing 鼻子锥的设计。而且,模拟被进行优化 morphing 鼻子锥的结构的参数。biomimetic 鼻子锥的这个概念图案将提供一个有效方法让太空车辆减少空气动力学拖。 | Jieliang Zhao Shaoze Yan Liren Deng He Huang Yueming Liu | 2017 | Journal of Bionic Engineering2017，14，2： | 6 |
| 3 | Generating high spatiotemporal resolution LAI based on MODIS/GF-1 data and combined Kriging-Cressman interpolation显示文摘Generation of high spatial and temporal resolution LAI(leaf area index)products is challenging because higher spatial resolution remotely sensed data usually have coarse temporal resolutions and vice versa.In this study,a novel method that combining Kriging interpolation and Cressman interpolation was proposed to generate high spatial and temporal resolution LAI products by fusing Moderate Resolution Imaging SpectroRadiometer(MODIS)characterized by coarse spatial resolution and high temporal resolution and Gaofen-1(GF-1)with fine spatial resolution and coarse temporal resolution.This method was applied to the Huangpu district of Guangzhou,Guangdong,China.The results showed that compared to field observation,the predicted values of LAI had an acceptable accuracy of 73.12%.Using Moran’s I index and Kolmogorov-Smirnov tests,it was found that the MODIS data were spatially auto-correlated and characterized by normal distributions.Scaling down the 1 km×1 km spatial resolution MODIS products to a spatial resolution of 30 m×30 m using point-Kriging resulted in a precision of 79.38%compared to the results at the same spatial resolution derived from an 8 m×8 m spatial resolution GF-1 image by scaling up using block-Kriging.Moreover,the regression models that accounts for the relationship between NDVI(Normalized Difference Vegetation Index)and LAI based on MODIS data obtained the determination coefficients ranging from 0.833 to 0.870.Finally,the data fusion and interpolation of MODIS and GF-1 data using Cressman method generated high spatial and temporal resolution LAI maps,which showed reasonably spatial and temporal variability.The results imply that the proposed method is a powerful tool to create high spatial and temporal resolution LAI products. | Liu Zhenhua Huang Rugen Hu Yueming Fan Shudi Feng Peihua | 2016 | International Journal of Agricultural and Biological Engineering2016，9，5： | 3 |
| 4 | Functional characterization of oligopeptide transporter 1 of dairy cows显示文摘Background: It is wel known that peptides play a vital role in the nutrition and health of dairy cows. Bovine oligopeptide transporter 1(bP epT 1) is involved in the peptide transport process in the gastrointestinal tracts of dairy cows. However,little information is known in the characteristics of bP epT 1. Therefore, the purpose of this study was to characterize bP epT 1 functional y using a mammalian cel expression system. The uptake of radiolabeled dipeptide glycyl-sarcosine([3 H]-Gly-Sar)into the bP epT 1-transfected Chinese hamster ovary cel s was measured at various pH and substrate concentrations and with or without 15 other smal peptides that contained Met or Lys.Results: Western blot results showed that the abundance of bP epT 1 protein in the jejunum and ileum are the highest in the gastrointestinal tract of dairy cows. The uptake of [3 H]-Gly-Sar by b Pep T1-Chinese hamster ovary cells was dependent on time, p H, and substrate concentration, with a low Kmvalue of 0.94 ± 0.06 mmol/L and a maximum velocity of 20.80 ± 1.74 nmol/(mg protein · 5 min). Most of the di-and tripeptides were the substrates of b Pep T1,based on substrate-competitive studies. However, bP epT 1 has a higher affinity to the peptides with shorter chains, greater hydrophobicity, and negative or neutral charges.Conclusions: These results demonstrated for the first time the functional characteristics of bP epT 1, and they provide a new insight and better understanding into its vital role in absorbing a wide range of peptides from the digestive tract of dairy cows. | Qingbiao Xu Zhixuan Liu Hongyun Liu Fengqi Zhao Xinbei Huang Yueming Wu Jianxin Liu | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 2 |
| 5 | An Integrated Dual-Band SiGe HBT Low Noise Amplifier 显示文摘 | Chenyang Huang and Yueming sin | 2005 | IEEE International Workshop on Radio-Frequency Integration Technology2005，，： | 1 |
| 6 | Application of Isotope Tracer Technique in Source Apportionment of Heavy Metal Pollution in Soil显示文摘The formation of heavy metal pollution in soil is closely related to human production and life. In order to effectively control heavy metal pollution and repair damaged soil,the pollution source should be known and judged first. Based on the preliminary analysis of major sources of soil heavy metal pollution in soil,combined with relevant literatures on heavy metal pollution source of soil or sediment at home and abroad in recent years,application progress of isotope tracer technique,especially lead isotope tracer technique,in the study of heavy metal pollution sources in soils and sediments were reviewed. The key points of future isotope tracer technology in the field of heavy metal pollution source apportionment were prospected. | Lin Ying Ke Nafen Chen Longjie Xiao Yueming Huang Huabin | 2017 | Plant Diseases and Pests2017，8，5： | 1 |
| 7 | Optimized selection of suitable sites for farmland consolidation projects using multi-objective genetic algorithms显示文摘In order to select suitable sites for farmland consolidation projects,correlation analysis and evolutionary algorithms were used to optimize the evaluation of ecological,social and economic factors,avoiding subjective selection and ignorance of spatial relationships among land attributes.Multi-objective Genetic Algorithms(MOGA)were applied to select the best sites from the perspective of spatial relationship and land attribute evaluation.With carefully defined restrictions and variables,multi-objective optimization is able to select several suitable sites for farmland consolidation projects.The results from a case study in Yangshan,Guangdong of China showed that the selected sites were on the central and southern Yangshan with expected flat terrain and abundant water resources.An empirical experiment also demonstrated that the proposed method is able to provide well selected sites for land consolidation projects. | Wang Lu Huang Ningsheng Kuang Yaoqiu Zhou Jinhao Zhao Yuan Zhang Zhen Hu Yueming | 2014 | International Journal of Agricultural and Biological Engineering2014，7，3： | 1 |
| 8 | Experience and inspirations of the Mass Drug Administration Programme with artemisinin-piperaquine in Moheli Island of the Comoros assisted by China显示文摘Malaria is one of the major diseases threatening the lives of people in Africa.Over the past decade,China has provided anti-malaria assistance to Africa.In November 2007,fast control malaria team of Guangzhou University of Chinese Medicine cooperated with the Comoros health authority.In Moheli Island of the Comoros,the anti-malaria team adopted a new strategy of Mass Drug Administration(MDA)and active intervention.They established an e ective anti-malaria system and reporting system,as well as a local anti-malaria team.Furthermore,they treated patients with malaria in Moheli Island and implemended health education in local people.In a very short time,they have achieved remarkable results.This paper summarized the experiences of the project in order to provide useful reference for China to better carry out anti-malaria action in Africa and expand foreign health assistance. | Qi Wang Yueming Yun Hongying Zhang Jiawen Guo Wanting Wu Changsheng Deng Bo Huang Zhiyong Xu Qin Xu Jianping Song | 2018 | Global Health Journal2018，2，3： | 1 |
| 9 | The antimicrobial peptide PFR induces necroptosis mediated by ER stress and elevated cytoplasmic calcium and mitochondrial ROS levels: cooperation with Ara-C to act against acute myeloid leukemia显示文摘Dear Editor,Antimicrobial peptides(AMPs)are an ancient class of short polypeptides present in a large number of species in nature with a variety of functions.1 PFR(PFWRIRIRR-NH 2)is one kind of AMP identified among the derivatives of lactoferrin.2 Our previous results showed that PFR inhibited the proliferation of human acute myeloid leukemia(AML)HL60 cells potentially without toxicity against normal cells.In addition,PFR induced necrosis by membrane disruption detected using scanning electron micro-scopy.3 However,the underlying mechanisms of these effects are not clearly understood. | Yudie Lv Gang Shao Qiyu Zhang Xi Wang Yueming Meng Lingfei Wang Feiyan Huang Tianxin Yang Yuanting Jin Caiyun Fu | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 1 |
| 10 | Fe–N–C single atom catalysts for the electrochemical conversion of carbon,nitrogen and oxygen elements显示文摘Single atom catalysts(SACs)are constituted by isolated active metal centers,which are heterogenized on inert supports such as graphene,porous carbon,and amorphous carbon.The thermal stability,electronic properties,and catalytic activities of the metal center can be controlled via manipulating the neighboring heteroatoms such as nitrogen,oxygen,and sulfur.Due to the atomical dispersion of the active catalytic centers,the amount of metal required for catalysis can be decreased.Furthermore,new possibilities are offered to easily control the selectivity of a given transformation process as well as to improve turnover frequencies and turnover numbers of target reactions.Among them,Fe–N–C single atom catalysts own special electronic structure,and have been widely used in many fields of electrocatalysis.This review aims to summarize the synthesis of Fe–N–C based on anchoring individual iron atoms on carbon/graphene.The spin-related properties of Fe–N–C catalysts are described,including the relation between spin and electron structure of Fe–N x as well as the coupling between electronic structure of Fe–N x and electronic(orbit)of CO_(2),N_(2)and O_(2).Next,mechanistic investigations conducted to un-derstand the specific behavior of Fe–N–C catalysts are highlighted,including C,N,O electro-reduction.Finally,some issues related to the future developments of Fe–N–C are put forward and corresponding feasible solutions are offered. | Jian Huang Qiao Zhang Jie Ding Yueming Zhai | 2022 | Materials Reports(Energy)2022，2，3： | 1 |
| 11 | Glycation promoted by dynamic high pressure microfluidisation pretreatment revealed by high resolution mass spectrometry显示文摘 | Xiaoqin Huang Zongcai Tu Hui Wang Qiuting Zhang Yueming Hu Lan Zhang Peipei Niu Yan Shi Hui Xiao | 2013 | Food Chemistry2013，，3： | 1 |
| 12 | A 12-month clinical survey of incidence and outcome of acute respiratory distress syndrome in Shanghai intensive care units显示文摘 | Yueming Lu Zhifang Song Xin Zhou Shaoguang Huang Duming Zhu Xingyi Yang C. Bai Bo Sun Roger Spragg Shanghai ARDS Study Group | 2004 | Intensive Care Medicine2004，，12： | 1 |
| 13 | Application of Cadmium Isotope Tracer Technique显示文摘Cadmium( Cd) is a strong carcinogenic heavy metal element. Because of its physical,chemical and toxicological characteristics,Cd environmental geochemical research has been concerned,and Cd detection technology is becoming more and more perfect. Cd isotope is one of the hotspots of non-traditional isotope research in recent years. One-way fractionation takes place in its evolution process,which makes the region or process significantly different and contains the information of its evolution environment and process,and Cd isotope tracer technique has been successfully applied to the study of the genesis of lead-zinc deposits,lunar soil and meteorites,marine environment and heavy metal pollution,and has become an important means to the analysis of the sources of Cd pollution. The determination technology of Cd and Cd isotope and its application were reviewed,and the future research points of Cd isotope technology were prospected. | ying lin yueming xiao nafen ke longjie chen huabin huang | 2018 | Agricultural Biotechnology2018，7，2： | 1 |
| 14 | Efficient preparation of silver nanoplates assisted by non-polar solvents 显示文摘 | HUANG Linjian ZHAI Yueming DONG Shaojun | 2009 | J Colloid Interface Sei2009，331，： | 1 |
| 15 | Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of sweet potato ( Ipomoea batatas L.) leaves flavonoid显示文摘 | Xiaoqin Huang Zongcai Tu Hui Xiao Zhi Li Qiuting Zhang Hui Wang Yueming Hu Lan Zhang | 2013 | Food and Bioproducts Processing2013，，1： | 1 |
| 16 | Experimental simulation of dolomite dissolution under burial diagenesis conditions and thermodynamic interpretation 显示文摘 | Huang Sijing Xiao Linping Yang Junjie Zhang Wenzheng Huang Yueming Liu Guixia | 2000 | Chinese J Geochem2000，19，1： | 1 |
| 17 | Distribution and Migration Patterns of Lead in Atmosphere-tea System by Isotopic Tracing Method显示文摘With low concentration of stable lead isotope as the tracer,using inductively coupled plasma mass spectrometry( ICP-MS),the lead absorption of tea plants as well as the distribution and migration pattern of lead in plants under different p H environments and spraying introduction of lead sources were studied through pot trial. The results showed that tea plants were very sensitive to atmospheric lead source pollution; atmospheric lead source pollution had a tendency of moving up to the top( bud); low p H conditions enhanced lead absorption of plants and promoted upward migration of lead in plants. The experimental method is closer to the actual situation that plants are exposed to low concentration pollution,and could distinguish experimentally introduced lead source from environmentally introduced lead source. | Huang Huabin Yang Miaofeng Lin Ying Ke Nafen Xiao Yueming Zhuang Zhixia Wang Xiaoru | 2017 | Plant Diseases and Pests2017，8，4： | 0 |
| 18 | Electrochemical quartz crystal microbalance study on Au-supported Pt adlayers for electrocatalytic oxidation of methanol in alkaline solution显示文摘Underpotential deposition(UPD) of Cu on an Au electrode followed by redox replacement reaction(RRR) of CuUPD with a Pt source(H2PtCl6 or K2PtCl4) yielded Au-supported Pt adlayers(for short,Pt(CuUPD-Pt4+)n/Au for H2PtCl6,or Pt(CuUPD-Pt2+)n/Au for K2PtCl4,where n denotes the number of UPD-redox replacement cycles).The electrochemical quartz crystal microbalance(EQCM) technique was used for the first time to quantitatively study the fabricated electrodes and estimate their mass-normalized specific electrocatalytic activity(SECA) for methanol oxidation in alkaline solution.In comparison with Pt(CuUPD-Pt2+)n/Au,Pt(CuUPD-Pt4+)n/Au exhibited a higher electrocatalytic activity,and the maximum SECA was obtained to be as high as 35.7 mA ?g?1 at Pt(CuUPD-Pt4+)3/Au.The layer-by-layer architecture of Pt atoms on Au is briefly discussed based on the EQCM-revealed redox replacement efficiency,and the calculated distribution percentages of bare Au sites agree with the experimental results deduced from the charge under the AuOx-reduction peaks.The EQCM is highly recommended as an efficient technique to quantitatively examine various electrode-supported catalyst adlayers,and the highly efficient catalyst adlayers of noble metals are promising in electrocatalysis relevant to biological,energy and environmental sciences and technologies. | HUANG Zhao,JIA Xue’En,XIE QingJi*,TAN YueMing,HUANG SuQing,HUANG JinHua,MENG Yue & YAO ShouZhuo* Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research,Ministry of Education College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China | 2010 | Science China Chemistry2010，53，11： | 0 |
| 19 | Ti_(3)C_(2)T_(x)MXene wrapped,carbon-coated porous Si sheets for improved lithium storage performance显示文摘Si-based materials have shown great potential as lithium-ion batteries(LIBs)anodes due to their natural reserves and high theoretical capacity.However,the large volume changes during cycles and poor conductivity of Si lead to rapid capacity decay and poor cycling stability,ultimately limiting their commercial applications.Herein,we have skillfully utilized the microporous MCM-22 zeolite as the unique silicon source to produce porous Si(pSi)sheets by a simple magnesiothermic reduction,followed by a carbon coating and further Ti_(3)C_(2)T_(x)MXene assembly,obtaining the ternary pSi@NC@TNSs composite.In the design,porous Si sheets provide more active sites and shorten Li-ion transport paths for electrochemical reactions.The N-doped carbon(NC)layer serves as a bonding layer to couple pSi and Ti_(3)C_(2)T_(x).The conductive network formed by 2D Ti_(3)C_(2)T_(x)and medium NC layer effectively enhances the overall charge transport of the electrode material,and helps to stabilize the electrode structure.Therefore,the as-made pSi@NC@TNSs anode delivers an improved lithium storage performance,exhibiting a high reversible capacity of 925 mAh/g at 0.5 A/g after 100 cycles.This present strategy provides an effective way towards high-performance Si-based anodes for LIBs. | Hui Cheng Yueming Liu Zhongling Cheng Xianying Wang Na Huang Haijiao Zhang | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
| 20 | Electrochemical quartz crystal microbalance study on the two-electrode-system cyclic voltammetric behavior of Prussian blue films显示文摘A two-channel electrochemical quartz crystal microbalance (EQCM) was used to investigate the cyclic voltammetric behavior of two Prussian blue (PB) film-modified Au electrodes in a two-electrode con-figuration in aqueous solution. The redox peaks observed in the two-electrode cyclic voltammogram (CV) are assigned to the intrinsic redox transitions among the Everitt's salt, PB, and Prussian yellow for the film itself, the redox process of the Au substrate and the redox process of small-quantity ferri-/ferrocyanide impurities entrapped in the PB film, as also supported by ultraviolet-visible (UV-Vis) spectroelectrochemical data. The profile of the two-electrode solid-state CV for the PB powder sand-wiched between two gold-coated indium-tin oxide (ITO) electrodes is similar to that for two PB-modified Au electrodes in aqueous solution, implying similar origins for the corresponding redox peaks. The two-channel EQCM method is expected to become a highly effective technique for the studies of the two-electrode electrochemical behaviors of many other species/materials. | TAN YueMing XIE QingJi HUANG JinHua CHEN DaiWu CHEN Xin TU XinMan LI YunLong GE Bin YAO ShouZhuo | 2008 | Science China Chemistry2008，51，11： | 0 |
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