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| 1 | 病原性丝状真菌的菌种保藏方法显示文摘目的探讨常见病原性丝状真菌的菌种保藏方法。方法将73株病原性丝状真菌经过纯化后,接种于马铃薯葡萄糖琼脂斜面分别在4℃和-80℃冷冻管保存,均用10%(v/v)的丙三醇作为保护剂。结果经过1 a左右的保存,将菌株复活,发现4℃斜面保藏法菌株的存活率为100%,-80℃冷冻管保藏法菌株的存活率为98.6%,有些毛癣菌属的菌株不耐低温而不适合-80℃保藏。结论病原真菌可以合理地结合上述2种方法保藏。若实验室配有低温冰箱,可以-80℃冷冻管保藏法为主,对于某些不耐低温的丝状真菌需要同时采用4℃斜面保藏法,则可以保证菌种的存活率。 | 吴婧 李东明 G.S.de Hoog 姚一建 | 2011 | 中国真菌学杂志2011，6，5： | 8 |
| 2 | 有机硒有助于母猪和断奶前仔猪的生长显示文摘在怀孕和哺乳母猪的日粮中可用硒酵母替代亚硒酸钠,以提高仔猪的体重和成活率。 | 魏长友 Danny M. Hooge | 2006 | 国外畜牧学(猪与禽)2006，，5： | 7 |
| 3 | Prokaryotical expression of structural and non-structural proteins of hepatitis G virus显示文摘AIM To study the epitope distribution of hepatitis G virus (HGV) and to seek for the potential recombinant antigens for the development of HGV diagnositic reagents.METHODS Fourteen clones encompassing HGV gene fragments from core to NS3 and NS5 were constructed using prokaryotic expression vector pRSET and (or)pGEX. and expressed in E. coli. Western blotting and ELISA were used to detect the immunoreactivity of these recombinant proteins.``RESULTS One clone with HGV fragment from core to El(Gl). one from E2 (G31), three from NS3 (G6, G61, G7),one from NS5B (G821) and one chimeric fragment from NS3and NS5B (G61 821) could be expressed well and showed obvious immunoreactivity by Western blotting.One clone with I-KGV framment from NS5B (G82) was also well expressed, but could not show immunoreactivity by Western blotting. No obvious expression was found in the other six clones. All the expressed recombinant proteins were in inclusion body form, except the protein G61 which could be expressed in soluble form. Further purified recombinant proteins Gl, G,31, G61, G821 and G61 821were detected in indirected ELISA as coating antigen respectively. Only recombinant Gl could still show immunoreactivity, and the other four recombinant proteins failed to react to the HGV antibody positive sera.Western blotting results indicated that the immunoactivity of these four recombinant proteins were lost during purification.``CONCLUSION Core to El, E2. NS3 and NS5 fragment of HGV contain antigenic epitopes, which could be produced in prokaryotically expressed recombinant proteins. A high. yield recombinant protein (Gl) located in HGV core to E1 could remain its epitope after purification, which showed the potential that G1 could be used as a coating antigen to develop an ELISA kit for HGV specific antibody diagnosis. | Ning-Shao Xia~1 Hai-Jie Yang~1 Jun Zhang~1 Chang-Qing Lin~1 Ying-Bin Wang~1 Juan Wang~1 Mei-Yun Zhan~2 MH Ng~3 1 Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen 361005,Fujian Province,China2 Institute of Virology,Chinese Academy of Preventive Medicine Beijing 100052,China3 Department of Microbiology,Hoog Kong University,Hongkong,China | 2001 | World Journal of Gastroenterology2001，7，5： | 4 |
| 4 | 荷兰环境协议:分担可持续工业发展的责任显示文摘工业界与政府共同努力,可以利用合作性协议来达到雄心勃勃的环境质量目标.这样的协议,只要能激励所涉及工业的革新性能力,就会比命令与控制思路更加卓有成效.政府在其颁发许可证时无法要求各公司开展有深远意义的革新,而且规定变革也不属于法规的性质,除非这些变革肯定能实现.各公司对它们自己的技术可能性要比法规当局清楚得多.然而,对于要取得成功的合作性协议来说。 | Maarten de Hoog | 1999 | 产业与环境1999，21，1： | 3 |
| 5 | 工厂化生产香肠肠衣腐烂黑斑中嗜盐细菌的分离鉴定及其特征特性显示文摘为工厂化生产的相关食品及农产品储存过程中微生物的鉴定及抑菌对策提供理论依据,采用高盐察氏液体培养基(Czapek Dox Broth)梯度盐浓度培养法,CDA(Czapek Dox Agar)及TSA(Trypticase Soy Agar)固体培养基进行分离纯化,16SrRNA基因测序,邻接法构建16SrRNA序列的系统发育树等方法,对工厂生产香肠肠衣腐烂黑斑中嗜盐细菌进行分离鉴定,研究其特征特性。结果表明:25%~30%盐浓度固体培养基中分离到3株嗜盐细菌(Gyfl1,Gyfl2,Gyfl3),经分子学分析鉴定Gyfl1为Halomonas variabilis,Gyfl2为Chromohalobacter israelensis,Gyfl3为Chromohalobacter canadensis;经药敏试验,庆大霉素、卡那霉素、利福平、红霉素和新霉素等常见抗生素均能抑制嗜盐菌株Gyfl1、Gyfl2和Gyfl3,而利福平抑菌效果更明显。 | 牟丽丽 涂云华 Syren de Hoog 刘涛华 吕倩 王颜颜 陈燕 康颖倩 | 2016 | 贵州农业科学2016，44，1： | 3 |
| 6 | The world’s ten most feared fungi显示文摘An account is provided of the world’s ten most feared fungi.Within areas of interest,we have organized the entries in the order of concern.We put four human pathogens first as this is of concern to most people.This is followed by fungi producing mycotoxins that are highly harmful for humans;Aspergillus flavus,the main producer of aflatoxins,was used as an example.Problems due to indoor air fungi may also directly affect our health and we use Stachybotrys chartarum as an example.Not everyone collects and eats edible mushrooms.However,fatalities caused by mushroom intoxications often make news headlines and therefore we include one of the most poisonous of all mushrooms,Amanita phalloides,as an example.We then move on to the fungi that damage our dwellings causing serious anxiety by rotting our timber structures and flooring.Serpula lacrymans,which causes dry rot is an excellent example.The next example serves to represent all plant and forest pathogens.Here we chose Austropuccinia psidii as it is causing devastating effects in Australia and will probably do likewise in New Zealand.Finally,we chose an important amphibian pathogen which is causing serious declines in the numbers of frogs and other amphibians worldwide.Although we target the top ten most feared fungi,numerous others are causing serious concern to human health,plant production,forestry,other animals and our factories and dwellings.By highlighting ten feared fungi as an example,we aim to promote public awareness of the cost and importance of fungi. | Kevin D.Hyde Abdullah M.S.Al-Hatmi Birgitte Andersen Teun Boekhout Walter Buzina Thomas L.Dawson Jr. Dan C.Eastwood E.B.Gareth Jones Sybren de Hoog Yingqian Kang Joyce E.Longcore Eric H.C.McKenzie Jacques F.Meis Laetitia Pinson-Gadais Achala R.Rathnayaka Florence Richard-Forget Marc Stadler Bart Theelen Benjarong Thongbai Clement K.M.Tsui | 2018 | Fungal Diversity2018，，6： | 2 |
| 7 | Experimental Validation of a New Ultrasound Method for the Simultaneous Assessment of Radial and Longitudinal Myocardial Deformation Independent of Insonation Angle显示文摘 | Stian Langeland Jan D’hooge Patrick F. Wouters H Alex Leather Piet Claus Bart Bijnens George R. Sutherland | 2005 | Circulation2005，，14： | 2 |
| 8 | 曲霉所致鼻面及鼻眶脑真菌病显示文摘鼻面及鼻眶脑真菌病(rhinofacial and rhino-orbital-cerebral mycosis,ROCM)是一种病死率高的暴发性真菌感染疾病,主要由毛霉目、虫霉目、蛙粪霉目真菌引起。曲霉属真菌多引起真菌性鼻窦炎,如果侵入邻近的鼻、面、眼眶组织,真菌可能会延伸到颅底和/或神经系统,从而危及生命。由于该类感染早期临床表现缺乏特异性,误诊率可能较高;此外,对曲霉的有效抗真菌治疗与其他真菌的抗真菌治疗有很大的不同。本文对PubMed和中文文献数据库的相关文献进行了系统的Meta分析,全面总结了引起ROCM的相关曲霉菌种类、分布、危险因素、临床表现、诊断和治疗。 | 胡建刚 李东明 G.Sybren de Hoog | 2020 | 菌物学报2020，39，12： | 2 |
| 9 | Naming and outline of Dothideomycetes-2014 including proposals for the protection or suppression of generic names显示文摘Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each fungal species can have one nomenclaturally correct name in a particular classification.All other previously used names for this species will be considered as synonyms.The older generic epithet takes priority over the younger name.Any widely used younger names proposed for use,must comply with Art.57.2 and their usage should be approved by the Nomenclature Committee for Fungi(NCF).In this paper,we list all genera currently accepted by us in Dothideomycetes(belonging to 23 orders and 110 families),including pleomorphic and nonpleomorphic genera.In the case of pleomorphic genera,we follow the rulings of the current ICN and propose single generic names for future usage.The taxonomic placements of 1261 genera are listed as an outline.Protected names and suppressed names for 34 pleomorphic genera are listed separately.Notes and justifications are provided for possible proposed names after the list of genera.Notes are also provided on recent advances in our understanding of asexual and sexual morph linkages in Dothideomycetes.A phylogenetic tree based on four gene analyses supported 23 orders and 75 families,while 35 families still lack molecular data. | Nalin N.Wijayawardene Pedro W.Crous Paul M.Kirk David L.Hawksworth Saranyaphat Boonmee Uwe Braun Dong-Qin Dai Melvina J.D’souza Paul Diederich Asha Dissanayake Mingkhuan Doilom Singang Hongsanan E.B.Gareth Jones Johannes Z.Groenewald Ruvishika Jayawardena James D.Lawrey Jian-Kui Liu Robert Lücking Hugo Madrid Dimuthu S.Manamgoda Lucia Muggia Matthew P.Nelsen Rungtiwa Phookamsak Satinee Suetrong Kazuaki Tanaka Kasun M.Thambugala Dhanushka N.Wanasinghe Saowanee Wikee Ying Zhang Andre Aptroot H.A.Ariyawansa Ali H.Bahkali D.Jayarama Bhat Cécile Gueidan Putarak Chomnunti G.Sybren De Hoog Kerry Knudsen Wen-Jing Li Eric H.C.McKenzie Andrew N.Miller Alan J.L.Phillips Marcin Piatek Huzefa A.Raja Roger S.Shivas Bernad Slippers Joanne E.Taylor Qing Tian Yong Wang Joyce H.C.Woudenberg Lei Cai Walter M.Jaklitsch Kevin D.Hyde | 2014 | Fungal Diversity2014，，6： | 2 |
| 10 | Applications of the Morris water maze in the study of learning and memory 显示文摘 | D' Hooge R De Deyn PP | 2001 | Brain Res Brain Res Rev2001，36，： | 1 |
| 11 | Gelatinase A and membrane-type 1 matrix metalloproteinase mRNA expressed in adenocortical cancer but not in adenomas 显示文摘 | Kjmlman M Enberg U Hoog A | 1999 | World J Surg1999，23，3： | 1 |
| 12 | Experimental valida- tion of a new ultrasound method for the simultaneous assessment of radial and longitudinal myocardial deformation independent of inso- nation angle显示文摘 | Langeland S D'hooge J Wouters PF | 2005 | Circulation2005，112，14： | 1 |
| 13 | Cerebral phaeohyphomycosis-a cure at what lengths? 显示文摘 | Li DM de Hoog GS | 2009 | The Lancet Infectious Diseases2009，9，6： | 1 |
| 14 | Redefinition of Aureoba- sidium puIlulans and its varieties显示文摘 | ZALAR P GOSTIN AR C IDE HooG Gs | 2008 | Studies in Mycology2008，61，1： | 1 |
| 15 | Acute changes in systolic and diastolic events during clinical coronary angioplasty:a comparison of regional velocity,strain rate,and strain measurement显示文摘 | Jamal F D'Hooge J | 2002 | J Am Soc Echocardiogr2002，15，1： | 1 |
| 16 | Portal venous flow and followup in patients with liver disease and healthy subjects显示文摘 | Vries PJ Hoekstra JBL Hooge P | 1994 | Seand J Gastroenterol1994，29，： | 1 |
| 17 | Experimental validation of a new ultrasound method for the simultaneous assessment of radial and longitudinal myocardial deformation independent of insonation angle显示文摘 | Langeland S Dhooge J Wounters P F | 2005 | Circulation2005，112，14： | 1 |
| 18 | Influence of Dietary Bacillus subtilis C-3102 Spores on Live Performance of Broiler Chickens in Four Controlled Pen Trials显示文摘 | D M Hooge H Ishimaru M D Sims | 2004 | Journal of Applied Poultry Research2004，13，： | 1 |
| 19 | Rapid screening for genotypes as possible markers of virulence in the neurotropie black yeast Exophiala dermatitidis using PCR-RFLP显示文摘 | Sudhadham M de Hoog GS Menken SB | 2010 | Jour- nal of Microbiological Methods2010，80，2： | 1 |
| 20 | Applications of the Morris water maze in the study of learning and memory显示文摘 | D'Hooge R De Deyn PP | 2001 | Brain Res Brain Res Rev2001，36，1： | 1 |
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