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| 1 | T cell–associated immunoregulation and antiviral effect of oxymatrine in hydrodynamic injection HBV mouse model显示文摘Although oxymatrine(OMT) has been shown to directly inhibit the replication of hepatitis B virus(HBV) in vitro, limited research has been done with this drug in vivo. In the present study, the antiviral effect of OMT was investigated in an immunocompetent mouse model of chronic HBV infection.The infection was achieved by tail vein injection of a large volume of DNA solution. OMT(2.2, 6.7 and20 mg/kg) was administered by daily intraperitoneal injection for 6 weeks. The efficacy of OMT was evaluated by the levels of HBV DNA, hepatitis B surface antigen(HBs Ag), hepatitis B e antigen(HBe Ag)and hepatitis B core antigen(HBc Ag). The immunoregulatory activity of OMT was evaluated by serum ELISA and flow cytometry. Results shows that OMT at 20 mg/kg inhibited HBV replication, and it was more efficient than entecavir(ETV) in the elimination of serum HBs Ag and intrahepatic HBc Ag. Inaddition, OMT accelerated the production of interferon-γ(IFN-γ) in a dose-dependent manner in CD4^+T cells. Our findings demonstrate the beneficial effects of OMT on the enhancement of immunological function and in the control of HBV antigens. The findings suggest this drug to be a good antiviral therapeutic candidate for the treatment of HBV infection. | Xiuxiu Sang Ruilin Wang Yanzhong Han Cong’en Zhang Honghui Shen Zhirui Yang Yin Xiong Huimin Liu Shijing Liu Ruisheng Li Ruichuang Yang Jiabo Wang Xuejun Wang Zhaofang Bai Xiaohe Xiao | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 20 |
| 2 | Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants显示文摘 | Lei Yang Xiaohui Zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li | 2018 | Protein & Cell2018，9，9： | 13 |
| 3 | Synchrotron radiation X-ray powder diffraction techniques applied in hydrogen storage materials--A review显示文摘Synchrotron radiation is an advanced collimated light source with high intensity. It has particular advantages in structural characterization of materials on the atomic or molecular scale. Synchrotron radiation X-ray powder diffraction(SR-XRPD) has been successfully exploited to various areas of hydrogen storage materials. In the paper, we will give a brief introduction on hydrogen storage materials, X-ray powder diffraction(XRPD), and synchrotron radiation light source. The applications of ex situ and in situ time-resolved SR-XRPD in hydrogen storage materials, are reviewed in detail. Future trends and proposals in the applications of the advanced XRPD techniques in hydrogen storage materials are also discussed. | Honghui Cheng Chen Lu Jingjing Liu Yongke Yan Xingbo Han Huiming Jin Yu Wang Yi Liu Changle Wu | 2017 | Progress in Natural Science:Materials International2017，27，1： | 5 |
| 4 | Dominant mutations in CHK1 cause pronuclear fusion failure and zygote arrest that can be rescued by CHK1 inhibitor显示文摘Dear Editor,Infertility poses a major challenge to human reproductive health,affecting approximately 48 million women worldwide.1 Assisted reproductive techniques(ART),including in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI),enable infertile women to have their biological embryos in vitro and further give birth to babies after embryo transfer.However,about 10%of all human embryos produced by ART are blocked in the early embryonic stage2 and approximately 2%fertilized oocytes derived from ART could not accomplish the first cell division.3 About one half of human infertility cases involve an underlying genetic factor,although the majority of genetic causes have remained elusive.4 In this study,we report that dominant mutations in CHK1 are responsible for pronuclear fusion failure and zygote arrest(PFF-ZA)in 7 out of 29 cases,likely through increasing the CHK1 activity.Importantly,PFF-ZA caused by these mutations could be effectively rescued by using the CHK1 inhibitor,PF477736. | Honghui Zhang Tailai Chen Keliang Wu Zhenzhen Hou Shigang Zhao Chuanxin Zhang Yuan Gao Ming Gao Zi-Jiang Chen Han Zhao | 2021 | Cell Research2021，31，7： | 4 |
| 5 | Superior electrochemical performance of La-Mg-Ni-based alloys with novel A_(2)B_(7)-A_(7)B_(23) biphase superlattice structure显示文摘Nickel metal hydride(Ni-MH) rechargeable batteries hold an important position in the new-energy vehicle market owing to their key technology advantages. Their negative electrode materials—hydrogen storage alloys(HSAs) are always on the spotlight and are the key to compete with the burgeoning Li-ion batteries. Here, for the first time we report a series of biphase supperlattice HSAs with a(La,Mg)_(2)Ni_7 matrix phase and a novel(La,Mg)_(7)Ni_(23) secondary phase. The biphase alloys show discharge capacities of402–413 m Ahg^(-1) compared with 376–397 mAhg^(-1) of the other multi-or single-phase alloys. These values are among the highest for superlattice HSAs. In addition, the alloy with 15.4 wt.%(La,Mg)_(7)Ni_(23) phase exhibits good high rate dischargeability due to the proper compromise between the amount of crystal boundaries and equilibrium plateau voltage. The cycling stability of the biphase alloys is lower than that of the single-phase alloy but is till higher than the multiphase alloy. The novel superlattice biphase alloys with superior overall electrochemical properties are expected to inspire further design and development of HSAs as advanced electrode materials for power batteries. | Jingjing Liu Shuai Zhu Xiangyu Chen Jie Xu Lu Zhang Kai Yan Wei Chen Honghui Cheng Shumin Han | 2021 | Journal of Materials Science & Technology2021，，21： | 4 |
| 6 | Correction to: Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants显示文摘This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://gffzzeb6f7f563f66445dh6w0v0vb9fkww6bbb.ffgz.tsg.suse.edu.cn/ licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | Lei Yang Xiaohui Zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li | 2019 | Protein & Cell2019，10，9： | 0 |
| 7 | Rare inherited missense variants of POGZ associate with autism risk and disrupt neuronal development显示文摘Excess de novo likely gene-disruptive and missense variants within dozens of genes have been identified in autism spectrum disorder(ASD)and other neurodevelopmental disorders.However,many rare inherited missense variants of these high-risk genes have not been thoroughly evaluated.In this study,we analyzed the rare missense variant burden of POGZ in a large cohort of ASD patients from the Autism Clinical and Genetic Resources in China(ACGC)and further dissected the functional effect of diseaseassociated missense variants on neuronal development.Our results showed a significant burden of rare missense variants in ASD patients compared to the control population(P=4.6×10-5,OR=3.96),and missense variants in ASD patients showed more severe predicted functional outcomes than those in controls.Furthermore,by leveraging published large-scale sequencing data of neurodevelopmental disorders(NDDs)and sporadic case reports,we identified 8 de novo missense variants of POGZ in NDD patients.Functional analysis revealed that two inherited,but not de novo,missense variants influenced the cellular localization of POGZ and failed to rescue the defects in neurite and dendritic spine development caused by Pogz knockdown in cultured mouse primary cortical neurons.Significantly,L1CAM,an autism candidate risk gene,is differentially expressed in POGZ deficient cell lines.Reduced expression of L1cam was able to partially rescue the neurite length defects caused by Pogz knockdown.Our study showed the important roles of rare inherited missense variants of POGZ in ASD risk and neuronal development and identified the potential downstream targets of POGZ,which are important for further molecular mechanism studies. | Wenjing Zhao Jieqiong Tan Tengfei Zhu Jianjun Ou Ying Li Lu Shen Huidan Wu Lin Han Yanling Liu Xiangbin Jia Ting Bai Honghui Li Xiaoyan Ke Jingping Zhao Xiaobing Zou Zhengmao Hu Hui Guo Kun Xia | 2019 | Journal of Genetics and Genomics2019，46，5： | 0 |
| 8 | Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus显示文摘Following the outbreak of coronavirus disease 2019(COVID-19),several severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-related coronaviruses have been discovered.Previous research has identified a novel lineage of SARS-CoV-2-related CoVs in bats,including RsYN04,which recognizes human angiotensin-converting enzyme 2(ACE2)and thus poses a potential threat to humans.Here,we screened the binding of the RsYN04receptor-binding domain(RBD)to ACE2 orthologs from 52animal species and found that the virus showed a narrower ACE2-binding spectrum than SARS-CoV-2.However,the presence of the T484W mutation in the RsYN04 RBD broadened its range.We also evaluated 44 SARS-CoV-2antibodies targeting seven epitope communities in the SARS-CoV-2 RBD,together with serum obtained from COVID-19 convalescents and vaccinees,to determine their cross-reaction against RsYN04.Results showed that no antibodies,except for the RBD-6 and RBD-7 classes,bound to the RsYN04 RBD,indicating substantial immune differences from SARS-CoV-2.Furthermore,the structure of the RsYN04 RBD in complex with cross-reactive antibody S43 in RBD-7 revealed a potently broad epitope for the development of therapeutics and vaccines.Our findings suggest RsYN04 and other viruses belonging to the same clade have the potential to infect several species,including humans,highlighting the necessity for viral surveillance and development of broad anticoronavirus countermeasures. | Runchu Zhao Sheng Niu Pu Han Yue Gao Dezhi Liu Chunliang Luo Honghui Liu Bo Liu Yanli Xu Jianxun Qi Zhihai Chen Weifeng Shi Lili Wu George F.Gao Qihui Wang | 2023 | Zoological Research2023，44，6： | 0 |
| 9 | Degradation prediction of proton exchange membrane fuel cell stack using semi-empirical and data-driven methods显示文摘Degradation prediction of proton exchange membrane fuel cell(PEMFC)stack is of great significance for improving the rest useful life.In this study,a PEMFC system including a stack of 300 cells and subsystems has been tested under semi-steady operations for about 931 h.Then,two different models are respectively established based on semi-empirical method and data-driven method to investigate the degradation of stack performance.It is found that the root mean square error(RMSE)of the semi-empirical model in predicting the stack voltage is around 1.0 V,while the predicted voltage has no local dynamic characteristics,which can only reflect the overall degradation trend of stack performance.The RMSE of short-term voltage degradation predicted by the DDM can be less than 1.0 V,and the predicted voltage has accurate local variation characteristics.However,for the long-term prediction,the error will accumulate with the iterations and the deviation of the predicted voltage begins to fluctuate gradually,and the RMSE for the long-term predictions can increase to 1.63 V.Based on the above characteristics of the two models,a hybrid prediction model is further developed.The prediction results of the semi-empirical model are used to modify the input of the data-driven model,which can effectively improve the oscillation of prediction results of the data-driven model during the long-term degradation.It is found that the hybrid model has good error distribution(RSEM=0.8144 V,R2=0.8258)and local performance dynamic characteristics which can be used to predict the process of long-term stack performance degradation. | Yupeng Wang Kangcheng Wu Honghui Zhao Jincheng Li Xia Sheng Yan Yin Qing Du Bingfeng Zu Linghai Han Kui Jiao | 2023 | Energy and AI2023，11，1： | 0 |
| 10 | Enhanced genome editing to ameliorate a genetic metabolic liver disease through co-delivery of adeno-associated virus receptor显示文摘Genome editing through adeno-associated viral(AAV) vectors is a promising gene therapy strategy for various diseases,especially genetic disorders. However, homologous recombination(HR) efficiency is extremely low in adult animal models. We assumed that increasing AAV transduction efficiency could increase genome editing activity, especially HR efficiency, for in vivo gene therapy. Firstly, a mouse phenylketonuria(PKU) model carrying a pathogenic R408W mutation in phenylalanine hydroxylase(Pah) was generated. Through co-delivery of the general AAV receptor(AAVR), we found that AAVR could dramatically increase AAV transduction efficiency in vitro and in vivo. Furthermore, co-delivery of SaCas9/sgRNA/donor templates with AAVR via AAV8 vectors increased indel rate over 2-fold and HR rate over 15-fold for the correction of the single mutation in Pah;mice. Moreover, AAVR co-injection successfully increased the site-specific insertion rate of a 1.4 kb Pah cDNA by 11-fold, bringing the HR rate up to 7.3% without detectable global off-target effects. Insertion of Pah cDNA significantly decreased the Phe level and ameliorated PKU symptoms. This study demonstrates a novel strategy to dramatically increase AAV transduction which substantially enhanced in vivo genome editing efficiency in adult animal models, showing clinical potential for both conventional and genome editing-based gene therapy. | Shuming Yin Lie Ma Tingting Shao Mei Zhang Yuting Guan Liren Wang Yaqiang Hu Xi Chen Honghui Han Nan Shen Wenjuan Qiu Hongquan Geng Yongguo Yu Shichang Li Weishi Yu Mingyao Liu Dali Li | 2022 | Science China(Life Sciences)2022，65，4： | 0 |
| 11 | Improvement in hydrogen storage performance of Mg by mechanical grinding with molten salt etching Ti_(3)C_(2)Cl_(x)显示文摘Mg-based materials are currently a hot research topic as hydrogen storage materials due to their considerable theoretical hydrogen storage capacity.However,the kinetic performance of hydrogen absorption and desorption of Mg is too slow and requires high temperature,which seriously hinders the application of this material.MXene is a new type of two-dimensional material with significant role in improving thermodynamics and kinetics.In this experiment,a two-dimensional layered MXene containing Cl functional group was prepared by molten salt etching using the Ti-containing MAX phase as the raw material.Then different ratios of Ti_(3)C_(2)Cl_(x) were uniformly dispersed onto the surface of Mg by high energy ball milling.The samples were characterized by hydrogen absorption and desorption kinetics,SEM,XRD,XPS,and DSC to investigate the effect of Ti_(3)C_(2)Cl_(x) on the hydrogen absorption and desorption performance of Mg.The onset hydrogen absorption temperature can be reduced to room temperature and the hydrogen release temperature is reduced by 200℃ by doping Ti_(3)C_(2)Cl_(x).And there is also 5.4wt%hydrogen storage in the isothermal hydrogen absorption test at 400℃.The results of DSC demonstrate that the Ea of Mg+15wt%Ti_(3)C_(2)Cl_(x) was reduced by 12.6%compared to pristine Mg.TheΔH is almost invariable.The results of XPS show that the presence of multivalent Ti promotes electron transfer and thus improves the conversion between Mg^(2+)/Mg and H^(−)/H.This study provides a guideline for further improving the hydrogen absorption and desorption performance of Mg-based hydrogen storage materials. | Shuaicheng Hu Lijun Lv Guo Yang Wei Liu Hong Miao Honghui Cheng Xingbo Han Zifeng Lin | 2023 | Progress in Natural Science:Materials International2023，33，2： | 0 |
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