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| 1 | Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells显示文摘AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably.METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind Ⅲ/Not Ⅰ followed by ligation with pRc/CMV, or BamHI/EcoR Ⅰ followed by ligation with pSG5UTPL/Flag. After the plasmid vectors were cleaved with the correspond enzymes, the amplified segments were inserted into pRc/CMV or pSGSUTPL/Flag plasmid vectors with T4DNA ligase. KOZAK sequence was added before the initial ATG code of each fragment using specific primer. The inserted segments in the recombinant plasmids were sequenced after subcloning. BALB/c mice myeloma cells (SP2/0 cell line) were transfected with the recombinant plasmids. The expressions of the different recombinants were compared by Western-blot, using a monoclonal anti-HBs antibody as the primary antibody and peroxidase-labeled multi-linker as the secondary. Stable SP2/0-pRc/CMV-S or SP2/0- pRc/CMV-MS clones were established through clone screening with G418.RESULTS: Fragments with anticipated size were harvested after PCR. After recombination and screening, the sequences of the inserted segments in the recombinants were confirmed to be S, preS2S, preSl-preS2S and preSlS encoding genes,determined by sequencing. The results of Western-blot hybridization were positive for the anticipated proteins.Among them, pRc/CMV-S or pRc/CMV-MS demonstrated the highest expressing their respective antigen.CONCLUSION: Eight recombinant plasmids expressing S,M, L or preSlS proteins are obtained. For hepatitis surface antigen expression in eukaryotic cells, the vector pRc/CMV is superior to pSG5UTPL/Flag, and pRc/CMV-S and pRc/CMVMS are the most efficient in the pRc/CMV clones. SP2/0 cells stably expressing HBsAg are established, and may be used as target cells for evaluating the CTL activity of a DNA vaccine in vitro. | ShanQin HongTang Lian-SanZhao FangHe YongLin LiLiu Xiao-MeiHe | 2003 | World Journal of Gastroenterology2003，9，5： | 12 |
| 2 | The host type I interferon response to viral and bacterial infections显示文摘Type I interferons (IFN) are well studied cytokines with anti-viral and immune-modulating functions. Type I IFNsare produced following viral infections, but until recently, the mechanisms of viral recognition leading to IFN productionwere largely unknown. Toll like receptors (TLRs) have emerged as key transducers of type I IFN during viral infectionsby recognizing various viral components. Furthermore, much progress has been made in defining the signaling path-ways downstream of TLRs for type I IFN production. TLR7 and TLR9 have become apparent as universally importantin inducing type I IFN during infection with most viruses, particularly by plasmacytoid dendritic cells. New intracellularviral pattern recognition receptors leading to type I IFN production have been identified. Many bacteria can also inducethe up-regulation of these cytokines. Interestingly, recent studies have found a detrimental effect on host cells if type IIFN is produced during infection with the intracellular gram-positive bacterial pathogen, Listeria monocytogenes. Thisreview will discuss the recent advances made in defining the signaling pathways leading to type I IFN production. | AndreaK.PERRY GangCHEN DahaiZHENG HongTANG GenhongCHENG | 2005 | Cell Research2005，15，6： | 12 |
| 3 | Tissue microarray for high-throughput analysis of gene expression profiles in hepatocellular carcinoma显示文摘AIM: To study the expression profiles of HBsAg, HBcAg,p21WAF1/CIP1 (p21), Rb genes in hepatocellular carcinoma (HCC) and to investigate their roles in the hepatocarcinogenesis.METHODS: HCC tissue microarray containing 120-min tissues of 40 HCC cases was constructed. HBsAg, HBc Ag,p21 and Rb proteins were immunohistochemically stained by streptavidin-peroxidase conjugated method (S-P). The expression loss of these genes in cancerous, paracancerous tissues and adjacent normal liver tissues of 40HCCs were comparatively examined.RESULTS: The positive rate of HBsAg expression in cancerous tissues of 40 HCCs was 7.5%, which was lower than that in para-cancerous and adjacent normal liver tissues (x2 =12.774, P<0.01; x2= 18.442, P<0.01). The positive rate of HBcAg expression in cancerous tissues of 40 HCCs was 20.0%, which was also lower than that in para-cancerous and adjacent normal liver tissues(x2= 9.482, P<0.01; x2= 14.645, P<0.01). p21 protein deletion rate in cancerous tissues of 40 HCCs was 27.5%,which was higher than that in para-cancerous and adjacent normal liver tissues (x2 = 7.439, P<0.01; x2 = 11.174,P<0.01). p21 protein deletion correlated remarkably with the pathological grade of HCC (x2 = 0.072, P<0.05). Rb protein deletion rate in cancerous tissues of 40 HCCs was42.5%, which was also higher than that in para-cancerous and adjacent normal liver tissues (x2 = 10.551, P<0.01;x2= 18.353, P<0.01). Rb protein deletion rate did not correlate remarkably with tumor size or pathological grade of HCC (x2 = 0.014, P>0.05; x2 = 0.017, P>0.05).CONCLUSION: Expression deletion of HBsAg, HBcAg, p21and Rb proteins in HCCs may play important roles in the carcinogenesis of HCC. Tissue microarray is an effective high-throughput technique platform for cancer research. | KaiLiu Xue-ZhongLei Lian-SanZhao HongTang LiLIu PingFeng Bing-JunLei | 2005 | World Journal of Gastroenterology2005，11，9： | 7 |
| 4 | Inhibition of hepatitis B Virus replication by hepatocyte nuclear factor 4‐alpha specific short hairpin RNA显示文摘 | En‐QiangChen Tao‐YouZhou XingCheng HongTang | 2012 | Liver Int2012，，5： | 1 |
| 5 | ImagingDuration Embedded Dynamic Scheduling of Earth Observation Satellites for Emergent Events显示文摘 | XiaonanNiu HongTang LixinWu | 2015 | Mathematical Problems in Engineering2015，，： | 1 |
| 6 | Research of the dynamic characteristics on a new hydraulic system of electro-hydraulic hammer显示文摘 | Li Hongtang Lei Bufang | 2003 | Chinese Journal of Mechanical Engineering2003，，1： | 1 |
| 7 | Research of the dynamic characteristics on a new hydraulic system of electro-hydraulic hammer显示文摘 | Li Hongtang Lei Bufang | 2003 | Chinese Journal of Mechanical Engineering 20032003，6268，： | 1 |
| 8 | Numerical simulation of coal-fired particulate agglomeration under the action of gas-liquid two-phase jet显示文摘Because the conditions of agglomeration experiment have more influence and the operation is complex, the numerical simulation research has more advantages. Using the combination of PBM and DPM in fluent, numerical simulation of coal-fired particulate reunification was carried out. The results show that the jet effect will introduce the vortex in the agglomeration room to form the turbulence region, and the double jet will make the turbulence area larger and provide stronger turbulence pulsation. The introduction of water vapor jet can increase the adhesion between particles while strengthening turbulence, and better promote the agglomeration of fine particles. | Zhu Hongtang | 2019 | 科技经济导刊2019，，24： | 0 |
| 9 | 局部联合外用1%阿达帕林(达芙文)和单纯外用1%克林霉素治疗痤疮的疗效比较以及局部外用1%阿达帕林(达芙文)进行维持治疗的疗效观察显示文摘比较局部联合外用1%阿达帕林(达芙文)和1%克林霉素治疗痤疮的效果,并观察单纯外用1%阿达帕林(达芙文)进行维持治疗的效果。方法:共有300名Ⅱ—Ⅲ痤疮患者随机分成联合用药组和单纯克林霉素组,共治疗12周。联合用药组联合使用1%阿达帕林(达芙文)和1%克林霉素,对照组单纯使用1%克林霉素。联合用药组早晨外用一次1%克林毒素,晚上先用1%阿达帕林,2分钟后再使用1%克林霉素,而对照组早、晚各外用1%克林霉素一次。在维持治疗的疗效观察中,治疗组每晚外用1%阿达帕林一次共12周,对照组不用药。治疗开始和每次随方时,计算皮损总数、非炎症性皮损的数量和炎症性皮损的数量。结果:联合用药组的治疗效果较好。在维持治疗中,与第12周相比较,治疗组在第24周的皮损总数、非炎症性皮损的数量和炎症性皮损的数量分别下降了41.6%,41.7% and 40.8%,而对照组分别上升了92.1%,97.1% and 87.7%。结论:与单纯外用1%克林霉素相比,联合使用1%阿达帕林(达芙文)和1%克林霉素治疗痤疮在减少皮损数量等方向效果更好。在常规治疗后,需要用1%阿达帕林(达芙文)进行维持治疗。 | 张建中 李林峰 涂亚庭 郑捷 蔡林 袁珊 冯爱萍 HongTang | 2003 | 临床皮肤科杂志2003，32，B12： | 0 |
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